EP2255880B1 - Dispositif et procédé pour la prévention de bulles d'air dans une chambre d'hybridation - Google Patents

Dispositif et procédé pour la prévention de bulles d'air dans une chambre d'hybridation Download PDF

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Publication number
EP2255880B1
EP2255880B1 EP10172196.7A EP10172196A EP2255880B1 EP 2255880 B1 EP2255880 B1 EP 2255880B1 EP 10172196 A EP10172196 A EP 10172196A EP 2255880 B1 EP2255880 B1 EP 2255880B1
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EP
European Patent Office
Prior art keywords
pressure
hybridization
chamber
agitation
line
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Not-in-force
Application number
EP10172196.7A
Other languages
German (de)
English (en)
Other versions
EP2255880A2 (fr
EP2255880A3 (fr
Inventor
Wolfgang Streit
Gyoergy Wenczel
Waltraud Lamprecht
Heribert Eglauer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tecan Trading AG
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Tecan Trading AG
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Filing date
Publication date
Application filed by Tecan Trading AG filed Critical Tecan Trading AG
Priority claimed from EP05105498A external-priority patent/EP1614466A3/fr
Publication of EP2255880A2 publication Critical patent/EP2255880A2/fr
Publication of EP2255880A3 publication Critical patent/EP2255880A3/fr
Application granted granted Critical
Publication of EP2255880B1 publication Critical patent/EP2255880B1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/65—Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Definitions

  • DNA deoxyribose nucleic acid
  • microarrays of such samples provides research with an important technique for the simultaneous analysis of thousands of genes.
  • This technique involves the immobilization of DNA samples from many genes on a solid substrate surface, such as on a glass slide for a light microscope.
  • the DNA samples are preferably arranged in an array of sample spots or "spots", ie in a two-dimensional grid on the substrate, and it is later possible to deduce the origin of the corresponding DNA sample from a specific position within such an array.
  • DNA microarrays have established themselves as successful tools and devices for performing DNA hybridization have been continually improved (see, eg US 6,238,910 or EP 1 260 265 A1 the applicant of the current patent application).
  • These documents disclose a device for providing a hybridization space for the hybridization of nucleic acid samples on a slide.
  • These devices are designed to be movable relative to the slide and comprise an annular seal or sealing surface for closing the gap-shaped hybridization space with respect to the ambient air, wherein a surface of this slide is acted upon by the seal or sealing surface.
  • these devices include lines for feeding or discharging media into the hybridization space or out of the hybridization space as well as a sample feed.
  • An improved temperature control and a movement of the liquid with eg RNA patterns towards DNA samples immobilized on the slide is also disclosed.
  • the hybridization procedure (buffer preparation, pattern injection, program definition on the hybridization systems used) was carried out according to the technical instructions of Alopex (ALOPEX GmbH, Fritz-Hornschuch-Str. 9, D-95326 Kulmbach, Federal Republic of Germany). In each case two slides 27 were inserted into the hybridization systems SN22 and PT3 and processed at 43 ° C. or at 61 ° C. From the following processed samples the results are discussed: 43 ° C 43 ° C HS 400 SN22 HS 400 PT3 Slide # 29, # 31 Slide # 24, # 27 61 ° C 61 ° C HS 400 SN22 HS 400 PT3 Slide # 33, # 34 Slide # 35, # 37
  • a second membrane 33 ' is provided, which closes a second agitation space 30'.
  • the second diaphragm 33 'in this case serves to support the spring element arranged as a gas cushion in the outlet line 13.

Claims (5)

  1. Procédé pour la prévention de bulles d'air dans une chambre d'hybridation (5) essentiellement en forme de fente d'un système (1) destiné à hybrider des échantillons d'acides nucléiques, des protéines ou des sections de tissu immobilisés sur des lames porte-objets (27), le procédé consistant à :
    (a) préparer une chambre d'hybridation (5) essentiellement en forme de fente entre une lame porte-objets (27) et un couvercle (26), le couvercle (26) étant agencé de telle façon par rapport à la lame porte-objets (27) que la chambre d'hybridation (5) est fermée de façon hermétique par rapport à l'air ambiant ; et
    (b) remplir pour l'essentiel la chambre d'hybridation (5) préparée conformément à l'étape (a) d'un liquide ;
    caractérisé en ce que le procédé comprend en outre :
    (c) former une pression de chambre dans la chambre d'hybridation (5) avec un dispositif de pression du système (1) ;
    (d) maintenir cette pression pendant un processus d'hybridation à une valeur qui est au moins 100 mbar jusqu'à maximum 1.4 bar au-dessus de la pression atmosphérique normale qui règne dans l'air ambiant,
    (e) créer par à-coups une pression d'agitation dans une chambre de pression (34), la pression d'agitation étant 0.5 à 1 bar supérieure à la pression de la chambre, cette chambre de pression (34) étant agencée dans le couvercle (26) et étant séparée d'une chambre d'agitation (30) par une membrane (33), et la chambre d'agitation (30) étant raccordée à la chambre d'hybridation (5) par une conduite d'agitation (36) ; et
    (f) contrecarrer de façon élastique les différences de pression d'agitation exercées par le dispositif d'agitation (32) sur la chambre de pression (34) avec un élément de ressort qui est en liaison avec le volume de liquide utilisé pour l'hybridation et qui est réalisé en tant qu' une deuxième membrane (33') agencée dans le couvercle (26) ou comme volume d'un tuyau collecteur (15) ou d'une conduite de sortie (13) rempli d'un gaz.
  2. Procédé selon la revendication 1, caractérisé en ce que la pression dans la chambre est produite au moyen d'un liquide ou d'un gaz.
  3. Procédé selon la revendication 2, caractérisé en ce que le liquide pour produire la pression de la chambre est un fluide d'hybridation poussé par une pompe d'alimentation (8) dans une conduite de distribution (10) et par le biais de soupapes d'admission (12) contre le liquide présent dans la chambre d'hybridation (5).
  4. Procédé selon la revendication 2, caractérisé en ce que le gaz pour produire la pression dans la chambre est de l'air aspiré par une pompe d'alimentation (8) par le biais d'une soupape de ventilation (9) ou un gaz inerte stocké dans un réservoir (3), ce gaz étant pressé par le biais d'une conduite de distribution (10) et de soupapes d'admission (11) contre le liquide dans la chambre d'hybridation (5).
  5. Procédé selon la revendication 1, caractérisé en ce que la pression de la chambre, indépendamment des différences de pression d'agitation, est maintenue 0.1 jusqu'à maximum 1.4 bar au-dessus de la pression atmosphérique normale qui règne dans l'air ambiant.
EP10172196.7A 2005-06-21 2005-06-21 Dispositif et procédé pour la prévention de bulles d'air dans une chambre d'hybridation Not-in-force EP2255880B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP05105498A EP1614466A3 (fr) 2004-07-08 2005-06-21 Dispositif et procédé pour la prévention de bulles d'air dans une chambre d'hybridation

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP05105498.9 Division 2005-06-21
EP05105498A Division EP1614466A3 (fr) 2004-07-08 2005-06-21 Dispositif et procédé pour la prévention de bulles d'air dans une chambre d'hybridation

Publications (3)

Publication Number Publication Date
EP2255880A2 EP2255880A2 (fr) 2010-12-01
EP2255880A3 EP2255880A3 (fr) 2011-08-17
EP2255880B1 true EP2255880B1 (fr) 2014-05-28

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EP10172196.7A Not-in-force EP2255880B1 (fr) 2005-06-21 2005-06-21 Dispositif et procédé pour la prévention de bulles d'air dans une chambre d'hybridation

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9535082B2 (en) 2013-03-13 2017-01-03 Abbott Laboratories Methods and apparatus to agitate a liquid
USD822224S1 (en) 2013-03-13 2018-07-03 Abbott Laboratories Reagent kit with multiple bottles

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USD962471S1 (en) 2013-03-13 2022-08-30 Abbott Laboratories Reagent container
USD978375S1 (en) 2013-03-13 2023-02-14 Abbott Laboratories Reagent container
US10512911B1 (en) * 2018-12-07 2019-12-24 Ultima Genomics, Inc. Implementing barriers for controlled environments during sample processing and detection
CN111111481B (zh) * 2020-01-19 2024-01-23 防灾科技学院 一种痕量溶解三元混合气体标准溶液的制作系统及方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US6258534B1 (en) * 1998-03-02 2001-07-10 Bbi Bioseq, Inc. Pressure-controlled nucleic acid hybridization
DE69935230T2 (de) 1998-08-10 2007-12-20 Genomic Solutions, Inc., Ann Arbor Vorrichtung für thermische und flüssige zirkulation zur hybridierung von nukleinsäuren
US6186659B1 (en) 1998-08-21 2001-02-13 Agilent Technologies Inc. Apparatus and method for mixing a film of fluid
US6948843B2 (en) * 1998-10-28 2005-09-27 Covaris, Inc. Method and apparatus for acoustically controlling liquid solutions in microfluidic devices
US6458526B1 (en) 2000-01-28 2002-10-01 Agilent Technologies, Inc. Method and apparatus to inhibit bubble formation in a fluid
US6607907B2 (en) * 2000-05-15 2003-08-19 Biomicro Systems, Inc. Air flow regulation in microfluidic circuits for pressure control and gaseous exchange
ATE288316T1 (de) 2001-05-25 2005-02-15 Tecan Trading Ag System mit verfahrenseinheit zum hybridisieren von nukleinsäureproben, proteinen und gewebeschnitten
DE10258398A1 (de) * 2002-12-11 2004-08-12 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Verfahren zur Amplifikation von DNA

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9535082B2 (en) 2013-03-13 2017-01-03 Abbott Laboratories Methods and apparatus to agitate a liquid
USD815299S1 (en) 2013-03-13 2018-04-10 Abbott Laboratories Reagent kit with multiple bottles
USD822224S1 (en) 2013-03-13 2018-07-03 Abbott Laboratories Reagent kit with multiple bottles

Also Published As

Publication number Publication date
EP2255880A2 (fr) 2010-12-01
EP2255880A3 (fr) 2011-08-17

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