EP2238434A4 - Verfahren und vorrichtung zur partikeleinführung und -rückgewinnung - Google Patents

Verfahren und vorrichtung zur partikeleinführung und -rückgewinnung

Info

Publication number
EP2238434A4
EP2238434A4 EP09706657A EP09706657A EP2238434A4 EP 2238434 A4 EP2238434 A4 EP 2238434A4 EP 09706657 A EP09706657 A EP 09706657A EP 09706657 A EP09706657 A EP 09706657A EP 2238434 A4 EP2238434 A4 EP 2238434A4
Authority
EP
European Patent Office
Prior art keywords
recovery
methods
particle introduction
introduction
particle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09706657A
Other languages
English (en)
French (fr)
Other versions
EP2238434A1 (de
Inventor
Andrea Marziali
David John Broemeling
Dylan Corey Gunn
Peter Jason Eugster
Joel Pel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of British Columbia
Original Assignee
University of British Columbia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of British Columbia filed Critical University of British Columbia
Publication of EP2238434A1 publication Critical patent/EP2238434A1/de
Publication of EP2238434A4 publication Critical patent/EP2238434A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44713—Particularly adapted electric power supply
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44769—Continuous electrophoresis, i.e. the sample being continuously introduced, e.g. free flow electrophoresis [FFE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/125—Electrophoretic separation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N2001/4038—Concentrating samples electric methods, e.g. electromigration, electrophoresis, ionisation
EP09706657A 2008-02-01 2009-01-30 Verfahren und vorrichtung zur partikeleinführung und -rückgewinnung Withdrawn EP2238434A4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US683808P 2008-02-01 2008-02-01
US683708P 2008-02-01 2008-02-01
PCT/CA2009/000111 WO2009094772A1 (en) 2008-02-01 2009-01-30 Methods and apparatus for particle introduction and recovery

Publications (2)

Publication Number Publication Date
EP2238434A1 EP2238434A1 (de) 2010-10-13
EP2238434A4 true EP2238434A4 (de) 2011-06-22

Family

ID=40912209

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09706657A Withdrawn EP2238434A4 (de) 2008-02-01 2009-01-30 Verfahren und vorrichtung zur partikeleinführung und -rückgewinnung

Country Status (4)

Country Link
US (2) US8475641B2 (de)
EP (1) EP2238434A4 (de)
CA (1) CA2713313A1 (de)
WO (1) WO2009094772A1 (de)

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US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
JP2007526823A (ja) * 2004-02-02 2007-09-20 ザ ユニバーシティ オブ ブリティッシュ コロンビア 粒子を運動および濃縮させるためのスコダ泳動ならびに方法および装置
CA2496294A1 (en) * 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
WO2009094772A1 (en) 2008-02-01 2009-08-06 The University Of British Columbia Methods and apparatus for particle introduction and recovery
WO2010121381A1 (en) 2009-04-21 2010-10-28 The University Of British Columbia System and methods for detection of particles
EP2458004B1 (de) 2010-03-30 2017-03-15 The University Of British Columbia Systeme und Verfahren für verbesserte Scodaphorese
JP2012029676A (ja) * 2010-07-07 2012-02-16 Sony Corp 核酸濃縮回収用カートリッジ、核酸濃縮回収方法、及び該カートリッジの製造方法
CA2742327A1 (en) 2011-05-20 2012-11-20 The University Of British Columiba Systems and methods for enhanced scoda
WO2013026161A1 (en) * 2011-08-25 2013-02-28 The University Of British Columbia Systems and methods for enrichment and detection of particles
US20130048487A1 (en) * 2011-08-25 2013-02-28 The University Of British Columbia Systems and methods for enrichment and detection of particles
WO2013104994A2 (en) 2012-01-13 2013-07-18 The University Of British Columbia Multiple arm apparatus and methods for separation of particles
WO2013166444A2 (en) 2012-05-04 2013-11-07 Boreal Genomics Corp. Biomarker analysis using scodaphoresis
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins

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Also Published As

Publication number Publication date
WO2009094772A1 (en) 2009-08-06
WO2009094772A8 (en) 2009-10-08
EP2238434A1 (de) 2010-10-13
US20130277223A1 (en) 2013-10-24
CA2713313A1 (en) 2009-08-06
US20110048950A1 (en) 2011-03-03
US8852416B2 (en) 2014-10-07
US8475641B2 (en) 2013-07-02

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