EP2225037A1 - Microfluid storage device - Google Patents

Microfluid storage device

Info

Publication number
EP2225037A1
EP2225037A1 EP08857884A EP08857884A EP2225037A1 EP 2225037 A1 EP2225037 A1 EP 2225037A1 EP 08857884 A EP08857884 A EP 08857884A EP 08857884 A EP08857884 A EP 08857884A EP 2225037 A1 EP2225037 A1 EP 2225037A1
Authority
EP
European Patent Office
Prior art keywords
storage device
transport path
fluid
transport
storage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP08857884A
Other languages
German (de)
French (fr)
Inventor
Lutz Weber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thinxxs Microtechnology GmbH
Original Assignee
Thinxxs Microtechnology GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thinxxs Microtechnology GmbH filed Critical Thinxxs Microtechnology GmbH
Publication of EP2225037A1 publication Critical patent/EP2225037A1/en
Pending legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber

Definitions

  • the invention relates to a microfluidic storage device comprising at least one reservoir chamber formed by bulging a film or membrane for a fluid, a predetermined breaking point for forming an opening of the storage device and a transport path leading from the storage chamber to an opening of the storage device e.g. at an interface between the storage device and a microfluidic processing device.
  • Such a storage device is used in addition to the storage of the transport and / or the targeted release of fluids.
  • the processing device may e.g. be used for the analysis of fluids (gases and liquids) in medical diagnostics and analytics as well as environmental analysis.
  • a memory device of the kind mentioned in the opening paragraph is known from WO / 002007002480A2.
  • the predetermined breaking point breaks and the fluid can flow through a channel forming the transport path to the said opening.
  • a controlled dosage is impossible.
  • air bubbles form in the transport path when the fluid emerges in an abrupt manner because the air present in the transport channel can not be completely displaced.
  • the uncontrolled entrainment of the air bubbles significantly impairs the function of the fluid during further processing in the fluidic processing device.
  • the invention has for its object to provide a new microfluidic storage device of the type mentioned above, which allows a more accurate dosage of fluid quantities removable from it and in particular avoids the formation of air bubbles. Furthermore, further uses of the transport route are to be developed.
  • the storage device according to the invention which achieves this object is characterized in that the transport path can be opened to a transport channel in accordance with the fluid flow emerging from the storage chamber.
  • the transport path itself has virtually no volume when the storage chamber is closed. The widening to a channel takes place, preferably by the pressurized fluid itself only with the removal of the fluid from the reservoir.
  • the fluid e.g. a reagent to be processed in a flow cell, metered and free of bubbles remove from the storage device and the transport distance beyond, for example, to use as a valve.
  • the predetermined breaking point is arranged directly on the storage chamber and the transport path leads from the predetermined breaking point to the opening at the said interface.
  • the predetermined breaking point could be formed by the transport path itself, as explained below.
  • the transport path to abutting or applyable channel walls of which at least one wall is deformable by the fluid to form the transport channel.
  • the wall may be stretchable by the fluid for forming the transport channel.
  • the channel walls are each formed by a flexible film or membrane or by a flexible film and a rigid plate.
  • the said films or the film and the plate are not or weakly connected in the region of the transport path than in the adjacent areas.
  • the latter compound may be so weak that it breaks under the pressure of the fluid. In this way, the transport route itself can serve as a predetermined breaking point.
  • the storage device according to the invention may be integrated in said microfluidic processing device.
  • the transport route may include several sections between which e.g. a container is arranged to comprise.
  • This may be a measuring container or a container containing a reagent, in particular a dry reagent.
  • the transport paths of several storage containers have a common, e.g. from a mixing chamber to said opening at the interface leading portion.
  • the transport path may have a plurality of sections connected in parallel or in series, which may be e.g. from a distribution chamber to several openings at the interface.
  • FIG. 1 shows a first exemplary embodiment of a storage device according to the invention in a sectional side view
  • FIG. 2 shows the storage device of FIG. 1 in a plan view
  • FIG. 3 shows a detailed view of the storage device of FIGS. 1 and 2
  • FIG. 4 shows the storage device of FIG. 1 during the removal of a stored fluid
  • FIG. 5 shows a detailed view of the storage device shown in FIG. 4,
  • FIG. 6 shows an embodiment for a transport path of a storage device according to the invention in a cross section
  • Fig. 7 shows an embodiment of a storage chamber of a
  • FIG. 1 1 - 14 further embodiments of memory devices according to the invention in a plan view
  • 15 shows a storage device according to the invention with a transport path, which has a plurality of intermediate containers, in a side view
  • FIG. 16 shows a further exemplary embodiment of a memory device according to the invention
  • FIG. 18 shows an exemplary embodiment of a storage chamber of a storage device according to the invention.
  • a storage device for storing a fluid 1 shown in Fig. 1 is provided with a fluid 1, e.g. as a reagent-processing flow cell 2, which has a base plate 3 and a lower cover 4, connected.
  • the storage device comprises a storage chamber 5 for the fluid 1, which is formed by a deep-drawn bulge 6 in a film 7 and a bulge 6 covering, associated with the film 7 film 8.
  • the films 7 and 8 are connected to each other, except for the region of the storage chamber 5 and the region of a transport path 9 over its entire surface, e.g. welded or glued. In the region of the transport path 9 lie, as can be seen in particular from FIG. 3, the films 7 and 8 only to each other.
  • a narrow welding or adhesive area, which forms a predetermined breaking point 10 separates the interior of the storage chamber 5 from the transport path 9. Deviating from the embodiment described here, the films 7 and 8 outside the storage chamber and the transport path need not be connected to each other over their entire surface , It suffices a the supply chamber and the transport path limiting connection area, which withstands the pressurization more than the predetermined breaking point 10.
  • the transport path 9 leads to a passage opening 1 1 in the film 8, which is preferably congruent to a passage opening 26 in the base plate 3. From the predetermined breaking point 10, the width of the transport path decreases continuously up to the passage opening 1 1.
  • the storage device is bonded to the base plate 3 via the film 8.
  • the passage opening 26 in the base plate 3 leads to a channel 13 in the flow cell 2, which ends, for example, at a fluid chamber 1 receiving reaction chamber (not shown).
  • a fluid chamber 1 receiving reaction chamber (not shown).
  • the previously hermetically sealed storage chamber 5 is compressed according to arrow 14 (FIG. 4), the break-off point 10 breaking under the pressure of the fluid 1.
  • the pressurized fluid 1 opens up a transport channel 15 by deforming the film 7 under stretching in the region of the transport path 9, as shown in FIG. 5.
  • the fluid 1 finally passes through the passage openings 11 and 26 into the channel 13 covered by the film 4 in the flow cell 2.
  • the storage device described above also allows a very accurate dosage of individual, expressed from the storage chamber 5 subsets of fluid stored therein 1. If the pressure is withdrawn as indicated by arrow 14, then closes due to elastic restoring force of the film 7, the transport path and transferred into the flow cell Fluid flow comes to a halt.
  • the fluid flow could be interrupted by acting on the transport path 9 according to arrow 16 blocking element, in the simplest case in the form of a punch, and the transport path together with the films 7 and 8 against each other oppressive blocking element can be used as a valve, the removal of desired subsets allows the stored fluid supply.
  • the blocking element acts according to arrow 16 as a proportional valve.
  • the pressurized valve can form the cross-section of the transport path differently far, whereby the flow rate of the fluid can be controlled.
  • the valve function regardless of the strength and rigidity of the base plate, which otherwise to the blocking element Counter-contact forms, with the help of a second, from the opposite direction vorschiebbaren blocking element can be performed even more efficient.
  • the film 8 could be omitted and the film 7 are connected directly to the base plate 3, so that the bulge 6 and the transport path 9 are limited on one side directly by the base plate 3.
  • Fig. 6 shows an embodiment of a transport path 9a, which is formed by a film 7a and a film 8a, the films outside the transport distances 9a, as in the embodiment of FIG. 1 to 5, glued together or welded , Notwithstanding this embodiment, both films have room for deformation, in particular under elongation, so that they can form a transport channel 15a with both sides curved walls. Depending on the stiffness of the films 7a and 8a, a symmetrical or asymmetrical curvature may result.
  • a storage chamber 5b could be formed by two films 7b and 8b, each with a bulge 6b or 6b '.
  • the bulges may vary in shape and dimensions depending on the thermoforming tools used in cold or hot deep drawing.
  • the shape of the storage chamber may differ from the chamber shown in Figs. 1-5 and not round, but e.g. be elongated.
  • the flow cell has a stepped base plate 3c and a cover plate 17.
  • the storage device is integrated between the cover plate 17 and a resting on the base plate 3c layer 18 made of an elastomeric material.
  • an elastic membrane 19 forms a storage device.
  • the elastic membrane consists for example of a thermoplastic elastic elastomer and / or silicone material.
  • a transport path 9d, is bounded by the membrane 19 and a base plate 3d of the flow cell.
  • the exemplary embodiment of FIG. 10 differs from the preceding exemplary embodiments in that no through opening 26d that passes through the base plate is formed, but that a channel 13e directly adjoins a transport path 9e.
  • 1 1 shows a storage device with a storage chamber 5f and a transport line 9f in a plan view. Notwithstanding the preceding embodiments, the transport path is not rectilinear but curved, so that an outlet opening is arranged at a desired location.
  • Fig. 12 shows a storage device with a storage chamber 5g and a transport path 9g.
  • the transport path branches into sections 20 and 21, wherein the section 20 leads to an outlet opening 1 1 g and the section 21 to an outlet opening 1 Ig 1 .
  • the transport path in this case fulfills the function of a fluid distributor.
  • a storage device shown in FIG. 13 has two storage chambers 5h and 5h '.
  • the transport section 23 has a meandering shape, which assists the mixing of the two fluids.
  • the transport path therefore fulfills the function of a fluid mixer.
  • the transport path can be used for accurate metering and further transport of a defined amount of fluid (metering).
  • a reagent or sample amount is transferred into the transport channel until, for example, it has reached the through-opening 11 h, which can be monitored by visual observation in the case of a transparent flow cell, for example made of a transparent plastic.
  • the pressurization of the reagent is stopped and the transport fluid in the chamber 5h 'subjected to pressurization. This leads to the further transport of the fluid located in the transport section 23 and thus to the further carrying out a defined quantity of reaction. With the help of blocking elements, this process can be repeated until the pantries are completely emptied.
  • a storage device shown in FIG. 14 with a storage chamber 5i and a transport path 9i has an intermediate container arranged in the transport path, which is coated on the inside with a dry reagent. If the fluid flows through the intermediate container 24, the interior of which as well as that of the transport path opens up entirely through the fluid, the dry reagent is at least partially dissolved and transported in the fluid.
  • the achievable interior space of the intermediate container 24 can be set very flat in accordance with the liquid pressure which can be adjusted and adjusted by the pressurization 14 or setting by the blocking element 16, and influence the dissolving behavior of the dry reagent in the desired manner.
  • a storage device with a storage chamber 5 shown in Fig. 15 contains in a transport path 9j various containers 25, e.g. could be filled with different dry reagent materials.
  • the embodiments of transport routes shown in FIGS. 11 to 15 can be combined with each other.
  • the storage device thus assumes the functions of a flow cell.
  • a downstream processing device no longer has any flow cell functions, such as e.g. an electrical or electrochemical sensor connected downstream of the storage device.
  • a storage device having a storage chamber 5k shown in Fig. 16 is connected to a flow cell 2k.
  • a base plate 3k of the flow cell 2k is arranged on a film 7k, by the bulge of which the storage chamber 5k is formed.
  • the film 7k covers a channel 13k formed in the base plate 3k, which is connected via a passage opening 11k in connection with a transport path 9k of the storage device.
  • a covering film corresponding to the film 4 could be mounted on the side of the base plate facing away from the channel 13, and further channels could be formed there, which, viewed in the projection, can intersect with the channel 13.
  • additional functions can be achieved with the same production cost of the flow cell.
  • the thickness of the base plate 3k is greater than the height of the storage chamber 5k, the chamber is protected against improper handling, especially in stack storage of the storage device. The handling of the storage device is thus safer overall.
  • FIG. 17 shows different embodiments for predetermined breaking points, which extend immediately adjacent to a storage chamber over the entire width of a transport path and are designed as a welded or / and glued connection between two films.
  • the dimension of the welded connection indicated by arrows in FIG. 17a which is preferably between 0.01 and 5 mm, in particular 0.1 and 2 mm, is decisive for the required opening pressure.
  • the shape of the predetermined breaking point may deviate from a rectangle, e.g. have the arrow shape shown there. In this way, manufacturing technology easier to produce welds greater width can be formed without the required opening pressure increases proportionally with the width.
  • Fig. 18 shows a storage chamber 51 formed by sheets 71 and 81.
  • the sheets 71 and 81 are abutted and the volume enclosed by the sheets is zero.
  • the films 71 and 81 are stretched in accordance with the degree of filling as in the case of a filled bag.
  • the inclusion of the filling quantity is done by closing a last weld.
  • the storage chamber can be completely emptied and the force for emptying does not increase, as in the embodiments described above, with the degree of emptying.
  • the components of the devices described above are produced by mass production processes, wherein the films described are formed by deep drawing, base plates produced by injection molding and gluing or welding are used as connection technologies.
  • Suitable materials are, in particular, plastics, in particular plastic films, but also metals and metal foils and / or composite materials, such as e.g. PCB material.

Abstract

The invention relates to a microfluid storage device having at least one supply chamber (5) formed by bulging a film (7) or membrane (19), a target break point (10) for forming an opening in the supply chamber (5), and a transport path (9) leading from the supply chamber (5) to an opening (11) in the supply chamber, for example, an interface between the storage device and a microfluid processing unit (2). According to the invention, the initially closed transport path (9) may be opened to form a transport channel (15) corresponding to the fluid stream escaping from the supply chamber (5), preferably by the escaping fluid itself, which allows the fluid to be removed from the storage device in a bubble-free manner.

Description

Beschreibung: Description:
„Mikrof luidische Speichervorrichtung""Microfluidic Storage Device"
Die Erfindung betrifft eine mikrofluidische Speichervorrichtung mit wenigstens einer, durch Ausbuchtung einer Folie oder Membran gebildeten Vorratskammer für ein Fluid, einer Sollbruchstelle zur Bildung einer Öffnung der Speichervorrichtung und einer Transportstrecke, die von der Vorratskammer zu einer Öffnung der Speichervorrichtung z.B. an einer Schnittstelle zwischen der Speichervorrichtung und einer mikrofluidischen Verarbeitungseinrichtung führt.The invention relates to a microfluidic storage device comprising at least one reservoir chamber formed by bulging a film or membrane for a fluid, a predetermined breaking point for forming an opening of the storage device and a transport path leading from the storage chamber to an opening of the storage device e.g. at an interface between the storage device and a microfluidic processing device.
Eine solche Speichervorrichtung dient neben der Speicherung dem Transport und/oder der zielgerichteten Freigabe von Fluiden. In Verbindung mit der Verarbeitungseinrichtung kann sie z.B. für die Analyse von Fluiden (Gasen und Flüssigkeiten) in der medizinischen Diagnostik und Analytik sowie der Umweltanalytik verwendet werden.Such a storage device is used in addition to the storage of the transport and / or the targeted release of fluids. In connection with the processing device, it may e.g. be used for the analysis of fluids (gases and liquids) in medical diagnostics and analytics as well as environmental analysis.
Eine Speichervorrichtung der eingangs erwähnten Art ist aus der WO/002007002480A2 bekannt. Durch Ausübung von Druck auf eine flexible Wand der Vorratskammer bricht unter dem Druck des Fluids die Sollbruchstelle auf und das Fluid kann durch einen die Transportstrecke bildenden Kanal zu der ge- nannten Öffnung fließen. Beim plötzlichen Aufbrechen der Sollbruchstelle kommt es zu einer starken Druckschwankung und dadurch zum stoßweisen Austreten der Fluids. Eine gesteuerte Dosierung ist unmöglich. Ferner besteht die Gefahr, dass sich beim stoßweisen Austreten des Fluids in die Transportstrecke Luftblasen bilden, weil die im Transportkanal vorhandene Luft nicht vollständig verdrängt werden kann. Die unkontrollierte Mitführung der Luftblasen beeinträchtigt erheblich die Funktion des Fluids bei der Weiterverarbeitung in der fluidischen Verarbeitungseinrichtung. Der Erfindung liegt die Aufgabe zugrunde, eine neue mikrofluidische Speichereinrichtung der eingangs erwähnten Art zu schaffen, die eine genauere Dosierung daraus entnehmbarer Fluidmengen ermöglicht und insbesondere die Bildung von Luftblasen vermeidet. Ferner sollen weitere Nutzungsmöglichkeiten der Transport- strecke erschlossen werden.A memory device of the kind mentioned in the opening paragraph is known from WO / 002007002480A2. By exerting pressure on a flexible wall of the storage chamber, under the pressure of the fluid, the predetermined breaking point breaks and the fluid can flow through a channel forming the transport path to the said opening. When sudden breaking of the predetermined breaking point, there is a strong pressure fluctuation and thus the intermittent escape of the fluids. A controlled dosage is impossible. Furthermore, there is a risk that air bubbles form in the transport path when the fluid emerges in an abrupt manner because the air present in the transport channel can not be completely displaced. The uncontrolled entrainment of the air bubbles significantly impairs the function of the fluid during further processing in the fluidic processing device. The invention has for its object to provide a new microfluidic storage device of the type mentioned above, which allows a more accurate dosage of fluid quantities removable from it and in particular avoids the formation of air bubbles. Furthermore, further uses of the transport route are to be developed.
Die diese Aufgabe lösende Speichervorrichtung nach der Erfindung ist dadurch gekennzeichnet, dass die Transportstrecke entsprechend dem aus der Vorratskammer austretenden Fluidstrom zu einem Transportkanal aufschließbar ist.The storage device according to the invention which achieves this object is characterized in that the transport path can be opened to a transport channel in accordance with the fluid flow emerging from the storage chamber.
Erfindungsgemäß weist bei geschlossener Vorratskammer die Transportstrecke selbst praktisch kein Volumen auf. Die Aufweitung zu einem Kanal erfolgt, vorzugsweise durch das unter Druck stehende Fluid selbst erst mit der Entnahme des Fluids aus dem Vorratsbehälter. So lässt sich das Fluid, z.B. eine in einer Flusszelle zu verarbeitende Reagenzflüssigkeit, dosiert und blasenfrei aus der Speichereinrichtung entnehmen und die Transportstrecke darüber hinaus z.B. als Ventil nutzen.According to the invention, the transport path itself has virtually no volume when the storage chamber is closed. The widening to a channel takes place, preferably by the pressurized fluid itself only with the removal of the fluid from the reservoir. Thus, the fluid, e.g. a reagent to be processed in a flow cell, metered and free of bubbles remove from the storage device and the transport distance beyond, for example, to use as a valve.
Vorzugsweise ist die Sollbruchstelle unmittelbar an der Vorratskammer angeordnet und die Transportstrecke führt von der Sollbruchstelle zu der Öffnung an der ge- nannten Schnittstelle. Alternativ könnte die Sollbruchstelle durch die Transportstrecke selbst gebildet sein, wie weiter unter erläutert wird.Preferably, the predetermined breaking point is arranged directly on the storage chamber and the transport path leads from the predetermined breaking point to the opening at the said interface. Alternatively, the predetermined breaking point could be formed by the transport path itself, as explained below.
In einer bevorzugten Ausführungsform der Erfindung weist die Transportstrecke aneinander anliegende oder anlegbare Kanalwände auf, von denen wenigstens eine Wand durch das Fluid unter Bildung des Transportkanals verformbar ist.In a preferred embodiment of the invention, the transport path to abutting or applyable channel walls, of which at least one wall is deformable by the fluid to form the transport channel.
Insbesondere kann die Wand durch das Fluid zur Bildung des Transportkanals dehnbar sein.In particular, the wall may be stretchable by the fluid for forming the transport channel.
Vorzugsweise sind die Kanalwände jeweils durch eine flexible Folie bzw. Membran oder durch eine flexible Folie und eine steife Platte gebildet.Preferably, the channel walls are each formed by a flexible film or membrane or by a flexible film and a rigid plate.
Die genannten Folien oder die Folie und die Platte sind im Bereich der Transportstrecke nicht oder schwächer als in den angrenzenden Bereichen miteinander verbunden. Letztere Verbindung kann so schwach sein, dass sie unter dem Druck des Fluids aufbricht. Auf diese Weise kann die Transportstrecke selbst als Sollbruchstelle dienen. Die Speichervorrichtung nach der Erfindung kann in die genannte mikrofluidische Verarbeitungseinrichtung integriert sein.The said films or the film and the plate are not or weakly connected in the region of the transport path than in the adjacent areas. The latter compound may be so weak that it breaks under the pressure of the fluid. In this way, the transport route itself can serve as a predetermined breaking point. The storage device according to the invention may be integrated in said microfluidic processing device.
Die Transportstrecke kann mehrere Abschnitte, zwischen denen z.B. ein Behälter angeordnet ist, umfassen.The transport route may include several sections between which e.g. a container is arranged to comprise.
Hierbei kann es sich um einen Messbehälter oder einen eine Reagenz, insbesondere eine Trockenreagenz, enthaltenden Behälter handeln.This may be a measuring container or a container containing a reagent, in particular a dry reagent.
In weiterer Ausgestaltung der Erfindung weisen die Transportstrecken mehrerer Vorratsbehälter einen gemeinsamen, z.B. von einer Mischkammer zu der genannten Öffnung an der Schnittstelle führenden Abschnitt auf.In a further embodiment of the invention, the transport paths of several storage containers have a common, e.g. from a mixing chamber to said opening at the interface leading portion.
Ferner kann die Transportstrecke mehrere, zueinander parallel oder in Reihe ge- schaltete Abschnitte aufweisen, die z.B. von einer Verteilerkammer zu mehreren Öffnungen an der Schnittstelle führen.Furthermore, the transport path may have a plurality of sections connected in parallel or in series, which may be e.g. from a distribution chamber to several openings at the interface.
Die Erfindung wird nachfolgend anhand von Ausführungsbeispielen und der beiliegenden, sich auf diese Ausführungsbeispiele beziehenden Zeichnungen näher erläutert. Es zeigen:The invention will be explained in more detail with reference to embodiments and the accompanying drawings relating to these embodiments. Show it:
Fig. 1 ein erstes Ausführungsbeispiel für eine erfindungsgemäße Speichervorrichtung in einer geschnittenen Seitenansicht, Fig. 2 die Speichervorrichtung von Fig. 1 in einer Draufsicht, Fig.3 eine Detailansicht der Speichervorrichtung von Fig. 1 und 2,1 shows a first exemplary embodiment of a storage device according to the invention in a sectional side view, FIG. 2 shows the storage device of FIG. 1 in a plan view, FIG. 3 shows a detailed view of the storage device of FIGS. 1 and 2,
Fig. 4 die Speichervorrichtung von Fig. 1 während der Entnahme eines gespeicherten Fluids,FIG. 4 shows the storage device of FIG. 1 during the removal of a stored fluid, FIG.
Fig. 5 eine Detailansicht der in Fig. 4 gezeigten Speichervorrichtung,5 shows a detailed view of the storage device shown in FIG. 4,
Fig. 6 ein Ausführungsbeispiel für eine Transportstrecke einer Speichervorrichtung nach der Erfindung in einem Querschnitt,6 shows an embodiment for a transport path of a storage device according to the invention in a cross section,
Fig. 7 ein Ausführungsbeispiel für eine Vorratskammer einerFig. 7 shows an embodiment of a storage chamber of a
Speichervorrichtung nach der Erfindung in einer Schnittdarstellung,Storage device according to the invention in a sectional view,
Fig. 8 - 10 verschiedene Speichervorrichtungen nach der Erfindung, die in eine Flusszelle integriert sind, in einer geschnittenen Seitenansicht, Fig. 1 1 - 14 weitere Ausführungsbeispiele für Speichervorrichtungen nach der Erfindung in einer Draufsicht, Fig. 15 eine Speichervorrichtung nach der Erfindung mit einer Transportstrecke, die mehrere Zwischenbehälter aufweist, in einer Seitenansicht,8 to 10 different memory devices according to the invention, which are integrated into a flow cell, in a sectional side view, Fig. 1 1 - 14 further embodiments of memory devices according to the invention in a plan view, 15 shows a storage device according to the invention with a transport path, which has a plurality of intermediate containers, in a side view,
Fig. 16 ein weiteres Ausführungsbeispiel für eine Speichervorrichtung nach der Erfindung,16 shows a further exemplary embodiment of a memory device according to the invention,
Fig. 17 Ausführungsbeispiele für Sollbruchstellen, undFig. 17 embodiments for predetermined breaking points, and
Fig. 18 ein Ausführungsbeispiel für eine Vorratskammer einer erfindungsgemäßen Speichervorrichtung.18 shows an exemplary embodiment of a storage chamber of a storage device according to the invention.
Eine in Fig. 1 gezeigte Speichervorrichtung für die Speicherung eines Fluids 1 ist mit einer das Fluid 1 z.B. als Reagenz verarbeitenden Flusszelle 2, die eine Grundplatte 3 und eine untere Abdeckfolie 4 aufweist, verbunden.A storage device for storing a fluid 1 shown in Fig. 1 is provided with a fluid 1, e.g. as a reagent-processing flow cell 2, which has a base plate 3 and a lower cover 4, connected.
Die Speichervorrichtung umfasst eine Vorratskammer 5 für das Fluid 1 , welche durch eine tiefgezogene Ausbuchtung 6 in einer Folie 7 und eine die Ausbuchtung 6 abdeckende, mit der Folie 7 verbundene Folie 8 gebildet ist.The storage device comprises a storage chamber 5 for the fluid 1, which is formed by a deep-drawn bulge 6 in a film 7 and a bulge 6 covering, associated with the film 7 film 8.
Die Folien 7 und 8 sind bis auf den Bereich der Vorratskammer 5 sowie den Bereich einer Transportstrecke 9 über ihre gesamte Fläche miteinander verbunden, z.B. verschweißt oder verklebt. Im Bereich der Transportstrecke 9 liegen, wie insbesondere aus Fig. 3 hervorgeht, die Folien 7 und 8 nur aneinander an. Ein schmaler Schweiß- oder Klebebereich, der eine Sollbruchstelle 10 bildet, trennt den Innenraum der Vorratskammer 5 von der Transportstrecke 9. Abweichend von dem hier beschriebenen Ausführungsbeispiel brauchen die Folien 7 und 8 außerhalb der Vorratskammer und der Transportstrecke nicht über ihre gesamte Fläche miteinander verbunden sein. Es genügt ein die Vorratskammer und die Transportstrecke begrenzender Verbindungsbereich, welcher der Druckbeaufschlagung mehr als die Sollbruchstelle 10 standhält.The films 7 and 8 are connected to each other, except for the region of the storage chamber 5 and the region of a transport path 9 over its entire surface, e.g. welded or glued. In the region of the transport path 9 lie, as can be seen in particular from FIG. 3, the films 7 and 8 only to each other. A narrow welding or adhesive area, which forms a predetermined breaking point 10, separates the interior of the storage chamber 5 from the transport path 9. Deviating from the embodiment described here, the films 7 and 8 outside the storage chamber and the transport path need not be connected to each other over their entire surface , It suffices a the supply chamber and the transport path limiting connection area, which withstands the pressurization more than the predetermined breaking point 10.
Die Transportstrecke 9 führt zu einer Durchgangsöffnung 1 1 in der Folie 8, die zu einer Durchgangsöffnung 26 in der Grundplatte 3 vorzugsweise deckungsgleich ist. Von der Sollbruchstelle 10 an nimmt die Breite der Transportstrecke bis zu der Durchgangsöffnung 1 1 stetig ab. Die Speichervorrichtung ist mit der Grundplatte 3 über die Folie 8 verklebt.The transport path 9 leads to a passage opening 1 1 in the film 8, which is preferably congruent to a passage opening 26 in the base plate 3. From the predetermined breaking point 10, the width of the transport path decreases continuously up to the passage opening 1 1. The storage device is bonded to the base plate 3 via the film 8.
Die Durchgangsöffnung 26 in der Grundplatte 3 führt zu einem Kanal 13 in der Flusszelle 2, welcher z.B. an einer das Fluid 1 aufnehmenden Reaktionskammer (nicht gezeigt) endet. Zum Einbringen des gespeicherten Fluids 1 in die das Fluid verarbeitende Flusszelle 2 wird die bis dahin hermetisch verschlossene Vorratskammer 5 gemäß Pfeil 14 (Fig. 4) zusammengedrückt, wobei unter dem Druck des Fluids 1 die Sollbruchstelle 10 aufbricht. Das unter Druck stehende Fluid 1 erschließt sich einen Transportkanal 15, indem es im Bereich der Transportstrecke 9 die Folie 7 unter Dehnung verformt, wie dies in Fig. 5 dargestellt ist. Das Fluid 1 gelangt schließlich durch die Durchgangsöffnungen 1 1 und 26 hindurch in den durch die Folie 4 abgedeckten Kanal 13 in der Flusszelle 2.The passage opening 26 in the base plate 3 leads to a channel 13 in the flow cell 2, which ends, for example, at a fluid chamber 1 receiving reaction chamber (not shown). For introducing the stored fluid 1 into the fluid-processing flow cell 2, the previously hermetically sealed storage chamber 5 is compressed according to arrow 14 (FIG. 4), the break-off point 10 breaking under the pressure of the fluid 1. The pressurized fluid 1 opens up a transport channel 15 by deforming the film 7 under stretching in the region of the transport path 9, as shown in FIG. 5. The fluid 1 finally passes through the passage openings 11 and 26 into the channel 13 covered by the film 4 in the flow cell 2.
Indem das Ausgangsvolumen der Transportstrecke 9 bei hermetisch abgeschlossener Vorratskammer 5 bei null liegt und das aus der Vorratskammer unter Druck austretende Fluid 1 selbst erst das Innenvolumen der Transportstrecke 9 bildet und sich einen Transportkanal 15 erschließt, können sich in dem in die Flusszelle 2 übertretenden Fluidstrom keine Luftblasen bilden, welche die Verarbeitung oder/und Funktion des Fluids 1 in der Flusszelle 2 beeinträchtigen.By the initial volume of the transport path 9 at hermetically sealed storage chamber 5 is at zero and emerging from the storage chamber under pressure fluid 1 itself forms the inner volume of the transport path 9 and opens up a transport channel 15, in the flow cell 2 passing fluid flow no Form bubbles that affect the processing and / or function of the fluid 1 in the flow cell 2.
Vorteilhaft erlaubt die vorangehend beschriebene Speichervorrichtung ferner eine sehr genaue Dosierung einzelner, aus der Vorratskammer 5 ausgedrückter Teilmengen des darin gespeicherten Fluids 1. Wird der Druck gemäß Pfeil 14 zurückgenommen, so schließt sich infolge elastischer Rückstellkraft der Folie 7 die Transportstrecke und der in die Flusszelle überführte Fluidstrom kommt zum Erliegen. Alternativ könnte der Fluidstrom durch ein auf die Transportstrecke 9 gemäß Pfeil 16 einwirkendes Sperrelement, im einfachsten Fall in der Form eines Stempels, unterbrochen und die Transportstrecke zusammen mit dem die Folien 7 und 8 gegeneinander drückenden Sperrelement als Ventil genutzt werden, das die Entnahme gewünschter Teilmengen des gespeicherten Fluidvorrats ermöglicht.Advantageously, the storage device described above also allows a very accurate dosage of individual, expressed from the storage chamber 5 subsets of fluid stored therein 1. If the pressure is withdrawn as indicated by arrow 14, then closes due to elastic restoring force of the film 7, the transport path and transferred into the flow cell Fluid flow comes to a halt. Alternatively, the fluid flow could be interrupted by acting on the transport path 9 according to arrow 16 blocking element, in the simplest case in the form of a punch, and the transport path together with the films 7 and 8 against each other oppressive blocking element can be used as a valve, the removal of desired subsets allows the stored fluid supply.
Bleibt der Druck auf die Vorratskammer gemäß Pfeil 14 bestehen, so wirkt das Sperrelement gemäß Pfeil 16 als Proportionalventil. Je nach der Position des Sperrelements kann das unter Druck stehende Ventil den Querschnitt der Transportstrecke unterschiedlich weit ausbilden, wodurch sich die Fließgeschwindigkeit des Fluids steuern lässt.If the pressure remains on the storage chamber according to arrow 14, then the blocking element acts according to arrow 16 as a proportional valve. Depending on the position of the blocking element, the pressurized valve can form the cross-section of the transport path differently far, whereby the flow rate of the fluid can be controlled.
Wenn die Grundplatte 3 mit der Abdeckfolie 4 im Bereich des Sperrelements 16 einen Durchbruch aufweist, so kann die Ventilfunktion unabhängig von der Festigkeit und Steifigkeit der Grundplatte, die ansonsten zu dem Sperrelement ein Gegenlαger bildet, mit Hilfe eines zweiten, aus entgegengesetzter Richtung vorschiebbaren Sperrelements noch effizienter ausgeführt werden.If the base plate 3 with the cover 4 in the region of the locking element 16 has an opening, the valve function, regardless of the strength and rigidity of the base plate, which otherwise to the blocking element Counter-contact forms, with the help of a second, from the opposite direction vorschiebbaren blocking element can be performed even more efficient.
Abweichend von dem anhand von Rg. 1 bis 5 beschriebenen Ausführungsbeispiel könnte die Folie 8 entfallen und die Folie 7 direkt mit der Grundplatte 3 verbunden werden, so dass die Ausbuchtung 6 und die Transportstrecke 9 auf einer Seite direkt durch die Grundplatte 3 begrenzt sind.Notwithstanding the embodiment described with reference to Rg. 1 to 5, the film 8 could be omitted and the film 7 are connected directly to the base plate 3, so that the bulge 6 and the transport path 9 are limited on one side directly by the base plate 3.
In den weiteren Figuren sind gleiche oder gleichwirkende Teile mit derselben Be- zugszahl bezeichnet, wobei der betreffenden Bezugszahl der Buchstabe a, b usw. beigefügt ist.In the other figures, the same or equivalent parts are denoted by the same reference numeral, the relevant reference number is accompanied by the letter a, b and so on.
Fig. 6 zeigt ein Ausführungsbeispiel für eine Transportstrecke 9a, die durch eine Folie 7a und eine Folie 8a gebildet ist, wobei die Folien außerhalb der Transport- strecken 9a, wie bei dem Ausführungsbeispiel gemäß Fig. 1 bis 5, miteinander verklebt bzw. verschweißt sind. Abweichend von diesem Ausführungsbeispiel haben beide Folien Platz zur Verformung, insbesondere unter Dehnung, so dass sie einen Transportkanal 15a mit beidseitig gewölbten Wänden bilden können. Entsprechend der Steifigkeit der Folien 7a und 8a kann sich eine symmetrische oder asymmetrische Wölbung ergeben.Fig. 6 shows an embodiment of a transport path 9a, which is formed by a film 7a and a film 8a, the films outside the transport distances 9a, as in the embodiment of FIG. 1 to 5, glued together or welded , Notwithstanding this embodiment, both films have room for deformation, in particular under elongation, so that they can form a transport channel 15a with both sides curved walls. Depending on the stiffness of the films 7a and 8a, a symmetrical or asymmetrical curvature may result.
Ebenso könnte gemäß Fig. 7 eine Vorratskammer 5b durch zwei Folien 7b und 8b mit je einer Ausbuchtung 6b bzw. 6b' gebildet sein. Die Ausbuchtungen können in Form und Abmessungen unterschiedlich sein, je nach den beim kalten oder warmen Tiefziehen verwendeten Tiefziehwerkzeugen.Likewise, according to FIG. 7, a storage chamber 5b could be formed by two films 7b and 8b, each with a bulge 6b or 6b '. The bulges may vary in shape and dimensions depending on the thermoforming tools used in cold or hot deep drawing.
Die Form der Vorratskammer kann von der in den Fig. 1 bis 5 gezeigten Kammer abweichen und nicht rund sondern z.B. länglich sein.The shape of the storage chamber may differ from the chamber shown in Figs. 1-5 and not round, but e.g. be elongated.
Eine in Fig.8 gezeigte Speichervorrichtung mit einer Vorratskammer 5c und einer Transportstrecke 9c, die etwa der in Fig. 1 bis 5 beschriebenen Speichervorrichtung entspricht, ist in eine Flusszelle 2c integriert. Die Flusszelle weist eine gestufte Grundplatte 3c sowie eine Deckplatte 17 auf. Die Speichervorrichtung ist zwischen der Deckplatte 17 und einer auf der Grundplatte 3c aufliegenden Schicht 18 aus einem Elastomermaterial eingebunden.A memory device shown in Fig.8 with a storage chamber 5c and a transport path 9c, which corresponds approximately to the memory device described in FIGS. 1 to 5, is integrated into a flow cell 2c. The flow cell has a stepped base plate 3c and a cover plate 17. The storage device is integrated between the cover plate 17 and a resting on the base plate 3c layer 18 made of an elastomeric material.
Bei dem Ausführungsbeispiel von Fig. 9 bildet eine elastische Membran 19 eine Speichervorrichtung. Die elastische Membran besteht z.B. aus einem thermoplas- tischen Elastomer oder/und aus Silikonmaterial. Eine Transportstrecke 9d, ist durch die Membran 19 und eine Grundplatte 3d der Flusszelle begrenzt.In the embodiment of Fig. 9, an elastic membrane 19 forms a storage device. The elastic membrane consists for example of a thermoplastic elastic elastomer and / or silicone material. A transport path 9d, is bounded by the membrane 19 and a base plate 3d of the flow cell.
Das Ausführungsbeispiel von Fig. 10 unterscheidet sich von den vorangehenden Ausführungsbeispielen dadurch, dass keine durch die Grundplatte durchgehende Durchgangsöffnung 26d gebildet ist, sondern sich ein Kanal 13e unmittelbar an eine Transportstrecke 9e anschließt.The exemplary embodiment of FIG. 10 differs from the preceding exemplary embodiments in that no through opening 26d that passes through the base plate is formed, but that a channel 13e directly adjoins a transport path 9e.
Fig. 1 1 zeigt eine Speichervorrichtung mit einer Vorratskammer 5f und einer Trans- portstreck 9f in einer Draufsicht. Abweichend von den vorangehenden Ausführungsbeispielen ist die Transportstrecke nicht geradlinig sondern gekrümmt ausgebildet, so dass eine Austrittsöffnung an einer gewünschten Stelle angeordnet ist.1 1 shows a storage device with a storage chamber 5f and a transport line 9f in a plan view. Notwithstanding the preceding embodiments, the transport path is not rectilinear but curved, so that an outlet opening is arranged at a desired location.
Fig. 12 zeigt eine Speichervorrichtung mit einer Vorratskammer 5g und einer Transportstrecke 9g. Die Transportstrecke verzweigt sich in Abschnitte 20 und 21 , wobei der Abschnitt 20 zu einer Austrittsöffnung 1 1 g und der Abschnitt 21 zu einer Austrittsöffnung 1 Ig1 führt. Die Transportstrecke erfüllt in diesem Fall die Funktion eines Fluidverteilers.Fig. 12 shows a storage device with a storage chamber 5g and a transport path 9g. The transport path branches into sections 20 and 21, wherein the section 20 leads to an outlet opening 1 1 g and the section 21 to an outlet opening 1 Ig 1 . The transport path in this case fulfills the function of a fluid distributor.
Eine in Fig. 13 gezeigte Speichereinrichtung weist zwei Vorratskammern 5h und 5h' auf. Transportstrecken 9h und 9h' führen zu einer Mischkammer 22, von der ein gemeinsamer Transportstreckenabschnitt 23 zu einer Austrittsöffnung 1 1 h führt. Der Transportstreckenabschnitt 23 weist eine Mäanderform auf, welche die Durch- mischung der beiden Fluide unterstützt. Die Transportstrecke erfüllt daher die Funktion eines Fluidmischers.A storage device shown in FIG. 13 has two storage chambers 5h and 5h '. Transport routes 9h and 9h 'lead to a mixing chamber 22, from which a common transport path section 23 leads to an outlet opening 11h. The transport section 23 has a meandering shape, which assists the mixing of the two fluids. The transport path therefore fulfills the function of a fluid mixer.
Wird z.B. die Vorratskammer 5h mit einem Fluid in Form einer Reagenz oder Probe in die Vorratskammer 5h' mit einem dem Transport dienenden Fluid, z.B. Luft oder Gas, gefüllt, so kann die Transportstrecke zum genauen Abmessen und Weitertransportieren einer definierten Fluidmenge dienen (Metering). In diesem Fall wird im ersten Schritt eine Reagenz oder Probemenge in den Transportkanal überführt, bis sie z.B. die Durchgangsöffnung 1 1 h erreicht hat, was im Falle einer transparenten, z.B. aus einem transparenten Kunststoff bestehenden Flusszelle durch visuelle Beobachtung kontrolliert werden kann. Dann wird die Druckbeaufschlagung der Reagenz unterbrochen und das Transportfluid in der Kammer 5h' einer Druckbeaufschlagung unterworfen. Dies führt zum Weitertransport des sich im Transportstreckenabschnitt 23 befindenden Fluids und damit zum Weiter- trαnsport einer definierten Reαgenzmenge. Mit Hilfe von Sperrelementen lässt sich dieser Vorgang so oft wiederholen, bis die Vorratskammern vollständig entleert sind.If, for example, the storage chamber 5h is filled with a fluid in the form of a reagent or sample into the storage chamber 5h 'with a transporting fluid, eg air or gas, then the transport path can be used for accurate metering and further transport of a defined amount of fluid (metering). In this case, in the first step, a reagent or sample amount is transferred into the transport channel until, for example, it has reached the through-opening 11 h, which can be monitored by visual observation in the case of a transparent flow cell, for example made of a transparent plastic. Then, the pressurization of the reagent is stopped and the transport fluid in the chamber 5h 'subjected to pressurization. This leads to the further transport of the fluid located in the transport section 23 and thus to the further carrying out a defined quantity of reaction. With the help of blocking elements, this process can be repeated until the pantries are completely emptied.
Eine in Fig. 14 gezeigte Speichervoπichtung mit einer Vorratskammer 5i und einer Transportstrecke 9i weist einen in der Transportstrecke angeordneten Zwischenbehälter auf, der innenseitig mit einer Trockenreagenz beschichtet ist. Durchströmt das Fluid den Zwischenbehälter 24, dessen Innenraum sich ebenso wie derjenige der Transportstrecke insgesamt erst durch das Fluid erschließt, so wird die Trockenreagenz wenigstens teilweise aufgelöst und in dem Fluid mittransportiert. Vorteilhaft lässt sich der erschließbare Innenraum des Zwischenbehälters 24 entsprechend des wirkenden und durch die Druckbeaufschlagung 14 bzw. Einstellung durch das Sperrelement 16 einstellbaren Flüssigkeitsdrucks sehr flach einstellen, und das Auflöseverhalten der Trockenreagenz in gewünschter Weise beeinflussen.A storage device shown in FIG. 14 with a storage chamber 5i and a transport path 9i has an intermediate container arranged in the transport path, which is coated on the inside with a dry reagent. If the fluid flows through the intermediate container 24, the interior of which as well as that of the transport path opens up entirely through the fluid, the dry reagent is at least partially dissolved and transported in the fluid. Advantageously, the achievable interior space of the intermediate container 24 can be set very flat in accordance with the liquid pressure which can be adjusted and adjusted by the pressurization 14 or setting by the blocking element 16, and influence the dissolving behavior of the dry reagent in the desired manner.
Eine in Fig. 15 gezeigte Speichervorrichtung mit einer Vorratskammer 5] enthält in einer Transportstrecke 9j verschiedene Behälter 25, die z.B. mit verschiedenen Trockenreagenzmaterialien gefüllt sein könnten.A storage device with a storage chamber 5 shown in Fig. 15 contains in a transport path 9j various containers 25, e.g. could be filled with different dry reagent materials.
Die in den Fig. 1 1 bis 15 gezeigten Ausführungsformen von Transportstrecken lassen sich miteinander kombinieren. Die Speichervorrichtung übernimmt damit die Funktionen einer Flusszelle. Im Extremfall weist eine nachgeschaltete Verarbeitungseinrichtung gar keine Flusszellenfunktionen mehr auf, wie z.B. ein der Speichervorrichtung nachgeschalteter elektrischer oder elektrochemischer Sensor.The embodiments of transport routes shown in FIGS. 11 to 15 can be combined with each other. The storage device thus assumes the functions of a flow cell. In the extreme case, a downstream processing device no longer has any flow cell functions, such as e.g. an electrical or electrochemical sensor connected downstream of the storage device.
Eine in Fig. 16 gezeigte Speichervorrichtung mit einer Vorratskammer 5k ist mit einer Flusszelle 2k verbunden. Eine Grundplatte 3k der Flusszelle 2k ist auf einer Folie 7k angeordnet, durch deren Ausbuchtung die Vorratskammer 5k gebildet ist. Die Folie 7k deckt einen in der Grundplatte 3k gebildeten Kanal 13k ab, der über eine Durchgangsöffnung 1 1 k in Verbindung mit einer Transportstrecke 9k der Speichervorrichtung steht.A storage device having a storage chamber 5k shown in Fig. 16 is connected to a flow cell 2k. A base plate 3k of the flow cell 2k is arranged on a film 7k, by the bulge of which the storage chamber 5k is formed. The film 7k covers a channel 13k formed in the base plate 3k, which is connected via a passage opening 11k in connection with a transport path 9k of the storage device.
Eine der Folie 4 entsprechende Abdeckfolie könnte auf der dem Kanal 13 abgewandten Seite der Grundplatte angebracht und dort könnten weitere Kanäle ge- bildet sein, die sich in der Projektion gesehen, mit dem Kanal 13 kreuzen können. Somit lassen sich bei gleichem Fertigungsaufwand der Flusszelle zusätzliche Funktionen erreichen. Indem, wie im vorliegenden Fall, die Dicke der Grundplatte 3k größer als die Höhe der Vorratskammer 5k ist, ist die Kammer gegen unsachgemäße Handhabung, insbesondere bei Stapellagerung der Speichervorrichtung geschützt. Die Handhabung der Speichervorrichtung ist damit insgesamt sicherer.A covering film corresponding to the film 4 could be mounted on the side of the base plate facing away from the channel 13, and further channels could be formed there, which, viewed in the projection, can intersect with the channel 13. Thus, additional functions can be achieved with the same production cost of the flow cell. By, as in the present case, the thickness of the base plate 3k is greater than the height of the storage chamber 5k, the chamber is protected against improper handling, especially in stack storage of the storage device. The handling of the storage device is thus safer overall.
Rg. 17 zeigt unterschiedliche Ausführungen für Sollbruchstellen, die sich unmittelbar angrenzend an eine Vorratskammer über die gesamte Breite einer Transportstrecke ausdehnen und als Schweiß- oder/und Klebeverbindung zwischen zwei Folien ausgebildet sind. Die in Fig. 17a durch Pfeile angedeutete Abmessung der Schweißverbindung, die vorzugsweise zwischen 0,01 und 5mm, insbesondere 0,1 und 2mm liegt, ist maßgebend für den erforderlichen Öffnungsdruck. Wie aus Fig. 17b hervorgeht, kann die Form der Sollbruchstelle von einem Rechteck abweichen und z.B. die dort gezeigte Pfeilform aufweisen. Auf diese Weise lassen sich fertigungstechnisch leichter herstellbare Schweißnähte größerer Breite bilden, ohne dass der erforderliche Öffnungsdruck mit der Breite proportional ansteigt.FIG. 17 shows different embodiments for predetermined breaking points, which extend immediately adjacent to a storage chamber over the entire width of a transport path and are designed as a welded or / and glued connection between two films. The dimension of the welded connection indicated by arrows in FIG. 17a, which is preferably between 0.01 and 5 mm, in particular 0.1 and 2 mm, is decisive for the required opening pressure. As shown in Fig. 17b, the shape of the predetermined breaking point may deviate from a rectangle, e.g. have the arrow shape shown there. In this way, manufacturing technology easier to produce welds greater width can be formed without the required opening pressure increases proportionally with the width.
Fig. 18 zeigt eine durch Folien 71 und 81 gebildete Vorratskammer 51. Im entleerten, in Fig. 18a gezeigten Zustand liegen die Folien 71 und 81 aneinander an und das durch die Folien eingeschlossene Volumen liegt bei null. Im gefüllten Zustand gemäß Fig. 18b sind die Folien 71 und 81 entsprechend dem Füllungsgrad wie bei einem gefüllten Beutel gedehnt. Der Einschluss der Füllmenge erfolgt durch Verschließen einer letzten Schweißnaht. Vorteilhaft lässt sich die Vorratskammer vollständig entleeren und die Kraft zum Entleeren steigt nicht, wie bei den voran- gehend beschriebenen Ausführungsbeispielen, mit der Grad der Entleerung an.Fig. 18 shows a storage chamber 51 formed by sheets 71 and 81. In the deflated condition shown in Fig. 18a, the sheets 71 and 81 are abutted and the volume enclosed by the sheets is zero. In the filled state according to FIG. 18b, the films 71 and 81 are stretched in accordance with the degree of filling as in the case of a filled bag. The inclusion of the filling quantity is done by closing a last weld. Advantageously, the storage chamber can be completely emptied and the force for emptying does not increase, as in the embodiments described above, with the degree of emptying.
Vorteilhaft werden die Komponenten der vorangehend beschriebenen Vorrichtungen nach Massenfertigungsverfahren hergestellt, wobei die beschriebenen Folien mittels Tiefziehen geformt, Grundplatten durch Spritzgießen erzeugt und Kleben oder Schweißen als Verbindungstechnologien eingesetzt werden. Als Materialien eignen sich vor allem Kunststoffe, insbesondere Kunststofffolien aber auch Metalle und Metallfolien und/oder Verbundwerkstoffe, wie z.B. Leiterplattenmaterial. Advantageously, the components of the devices described above are produced by mass production processes, wherein the films described are formed by deep drawing, base plates produced by injection molding and gluing or welding are used as connection technologies. Suitable materials are, in particular, plastics, in particular plastic films, but also metals and metal foils and / or composite materials, such as e.g. PCB material.

Claims

Patentansprüche: claims:
1. Mikrofluidische Speichervorrichtung mit wenigstens einer, durch Ausbuchtung einer Folie (7) oder Membran (19) gebildeten Vorratskammer (5) für eine Fluid ( 1 ) , einer Sollbruchstelle ( 10) zur Bildung einer Öffnung der Vorratskammer (5) und einer Transportstrecke (9), die von der Vorratskammer (5) zu einer Öffnung (1 1 ) der Speichervorrichtung z.B. an einer Schnittstelle zwischen der Speichervorrichtung und einer mikrofluidischen Verarbeitungseinrichtung (2) führt, dadurch gekennzeichnet, dass die Transportstrecke (9) entsprechend dem aus der Vorratskammer (5) austretenden Fluidstrom zu einem Transportkanal (15) aufschließbar ist.1. A microfluidic storage device having at least one storage chamber (5) formed by bulging a film (7) or membrane (19) for a fluid (1), a predetermined breaking point (10) for forming an opening of the storage chamber (5) and a transport path (FIG. 9), from the storage chamber (5) to an opening (1 1) of the storage device, for example at an interface between the storage device and a microfluidic processing device (2), characterized in that the transport path (9) can be opened according to the fluid flow emerging from the storage chamber (5) to form a transport channel (15).
2. Speichervorrichtung nach Anspruch 1 , dadurch gekennzeichnet, dass die Transportstrecke (9 ) durch das aus der Vorratskammer (5) austretende Fluid (1 ) selbst zu dem Transportkanal (15) aufschließbar ist.2. Storage device according to claim 1, characterized in that the transport path (9) through the from the storage chamber (5) exiting fluid (1) itself to the transport channel (15) is unlockable.
3. Speichervorrichtung nach Anspruch 1 oder 2, dadurch gekennzeichnet, dass die Sollbruchstelle (10) unmittelbar an der Vorratskammer (5) angeordnet ist und die Transportstrecke (9) von der Sollbruchstelle (10) zu der Öffnung (1 1 ) an der Schnittstelle führt.3. Storage device according to claim 1 or 2, characterized in that the predetermined breaking point (10) is arranged directly on the storage chamber (5) and the transport path (9) from the predetermined breaking point (10) to the opening (1 1) leads to the interface ,
4. Speichervorrichtung nach einem der Ansprüche 1 bis 3, dadurch gekennzeichnet, dass die Transportstrecke (9) aneinander anliegende oder anlegbare Kanalwände umfasst, von denen wenigstens eine Wand durch das Fluid (1 ) unter Bildung des Transportkanals (15) verformbar ist.4. Storage device according to one of claims 1 to 3, characterized in that the transport path (9) abutting or engageable channel walls, of which at least one wall by the fluid (1) to form the transport channel (15) is deformable.
5. Speichervorrichtung nach Anspruch 4, dadurch gekennzeichnet, dass die Wand durch das Fluid (1 ) zur Bildung des Transportkanals (15) dehnbar ist.5. Storage device according to claim 4, characterized in that the wall through the fluid (1) to form the transport channel (15) is stretchable.
6. Speichervorrichtung nach Anspruch 4 oder 5, dadurch gekennzeichnet, dass die Kanalwände jeweils durch eine Folie (7,8) oder durch eine Folie (7) und eine steife Platte (3) gebildet sind.6. Storage device according to claim 4 or 5, characterized in that the channel walls are each formed by a foil (7, 8) or by a foil (7) and a rigid plate (3).
7. Speichervorrichtung nach Anspruch 6, dadurch gekennzeichnet, dass die Folien (7,8) oder die Folie (7) und die Platte (3) im Bereich der Transportstrecke (9) nicht oder schwächer als in den angrenzenden Bereichen miteinander verbunden sind.7. Storage device according to claim 6, characterized in that the films (7,8) or the film (7) and the plate (3) in the region of the transport path (9) are not or weakly connected to each other than in the adjacent areas.
8. Speichervorrichtung nach Anspruch 7, dadurch gekennzeichnet, dass die Speichervorrichtung in die Verarbeitungseinrichtung (2) integriert ist.8. Storage device according to claim 7, characterized in that the storage device is integrated in the processing device (2).
9. Speichervorrichtung nach einem der Ansprüche 1 bis 8, dadurch gekennzeichnet, dass die Transportstrecke (9) mehrere Abschnitte, zwischen denen z.B. ein Zwischenbehälter (22,24,25) angeordnet ist, umfasst.A memory device according to any one of claims 1 to 8, characterized in that the transport path (9) comprises a plurality of sections between which e.g. an intermediate container (22,24,25) is arranged comprises.
10. Speichervorrichtung nach einem der Ansprüche 1 bis 9, dadurch gekennzeichnet, dass die Transportstrecken mehrerer Vorratsbehälter (5h,5h') einen gemeinsamen, z.B. von einer Mischkammer (22) zu der Öffnung (l l h) an der Schnittstelle führenden Abschnitt (23) aufweisen.10. Storage device according to one of claims 1 to 9, characterized in that the transport paths of a plurality of storage containers (5h, 5h ') have a common, e.g. from a mixing chamber (22) to the opening (l l h) at the interface leading portion (23).
1 1. Speichervorrichtung nach einem der Ansprüche 1 bis 10, dadurch gekennzeichnet, dass die Transportstrecke (9g) mehrere zueinander parallel geschaltete, z.B. von einer Verteilerkammer, zu einer Öffnung (1 1 g,l 1 g') an der Schnittstelle führende Abschnitte (20,21 ) umfasst.A memory device according to any one of claims 1 to 10, characterized in that the transport path (9g) comprises a plurality of mutually parallel, e.g. from a distribution chamber, to an opening (1 1 g, l 1 g ') at the interface leading portions (20,21).
12. Speichervorrichtung nach einem der Ansprüche 1 bis 1 1 , dadurch gekennzeichnet, dass das Innenvolumen der Vorratskammer (51) im entleerten Zustand bei null liegt. 12. Storage device according to one of claims 1 to 1 1, characterized in that the inner volume of the storage chamber (51) is in the deflated state at zero.
EP08857884A 2007-12-06 2008-12-05 Microfluid storage device Pending EP2225037A1 (en)

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US9211538B2 (en) 2015-12-15

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