EP2123359A1 - Dispositif de pipetage pour l'aspiration et la distribution d'un fluide de dosage - Google Patents

Dispositif de pipetage pour l'aspiration et la distribution d'un fluide de dosage Download PDF

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Publication number
EP2123359A1
EP2123359A1 EP08008525A EP08008525A EP2123359A1 EP 2123359 A1 EP2123359 A1 EP 2123359A1 EP 08008525 A EP08008525 A EP 08008525A EP 08008525 A EP08008525 A EP 08008525A EP 2123359 A1 EP2123359 A1 EP 2123359A1
Authority
EP
European Patent Office
Prior art keywords
piston
cylinder
pipetting device
working
pipetting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP08008525A
Other languages
German (de)
English (en)
Other versions
EP2123359B1 (fr
Inventor
Armin Panzer
Patrick Schelling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hamilton Bonaduz AG
Original Assignee
Hamilton Bonaduz AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hamilton Bonaduz AG filed Critical Hamilton Bonaduz AG
Priority to AT08008525T priority Critical patent/ATE511921T1/de
Priority to ES08008525T priority patent/ES2364990T3/es
Priority to EP08008525A priority patent/EP2123359B1/fr
Priority to JP2009109567A priority patent/JP4855496B2/ja
Priority to US12/435,772 priority patent/US8245586B2/en
Publication of EP2123359A1 publication Critical patent/EP2123359A1/fr
Application granted granted Critical
Publication of EP2123359B1 publication Critical patent/EP2123359B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/146—Employing pressure sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/147—Employing temperature sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons

Definitions

  • the present invention relates to a pipetting device for aspirating and dispensing a dosing fluid by means of a different working fluid, wherein the working fluid is housed in a variable volume working space extending along a channel axis and at least at least along an axial portion thereof with respect to the channel axis is formed by a piston-cylinder system, with a cylinder bounding the working chamber along a cylinder section in the radial direction and with a piston bounding the working chamber in a first axial direction, wherein the cylinder and piston are arranged to be movable relative to one another, so that the piston Cylinder system has an open for aspiration and dispensing axial dosing L jossend Scheme and an axial working longitudinal end region closed by the piston.
  • Such pipetting devices are for example from the EP-A-1 745 851 or the EP-A-1 412 759 known. From these documents is also known to measure the pressure of the working fluid in order to draw conclusions about the proper course of a performed aspiration and / or dispensation can.
  • Pipetting devices of the type mentioned above are used for high-precision dosing of fluids, especially liquids, in laboratories and in industry.
  • the amount of absorbable, ie aspiratable dosing fluid is limited by the maximum possible change in the volume of the working space of the pipetting device.
  • pipetting devices Since usually pipetting devices are used in which a plurality of so-called “pipetting", each having a working space, are arranged in rows and columns matrix-like, the pipetting a channel forming components are miniaturized, so that not only the workrooms seen an absolute Having small volume, but also the relative movement of the piston and cylinder of the piston-cylinder system allows only a small change in working volume, which limits the aspirating and dispensing volume of dosing to the top.
  • the volume of the involvement of the piston with limited working space is changed to a greater extent than if the piston, as known from the prior art, an inner piston, which in the interior of the cylinder at the Cylinder inner wall is guided.
  • the difference in the working volume change at the same stroke of a pipetting device of the present invention in comparison with the prior art corresponds to the volume from the cross-sectional area of the cylinder wall multiplied by the stroke.
  • the working space is formed at least along an axial portion of the working space by a piston-cylinder system
  • a piston-cylinder system contributes to the formation of the working space.
  • pipetting tips and the like which likewise contribute to the volume of the working space in the case of their coupling, can be coupled to the piston-cylinder system.
  • the piston-cylinder system is limited by the piston in the first axial direction from the interior of the piston-cylinder system and is open in a second axial direction opposite to the first direction, so that in this second direction one by enlargement or reduction the working space caused pressure change in the working fluid can act on the dosing and this can thus be directed into the working space or out of this.
  • channel axis is usually a straight-line channel axis. However, it should not be excluded that the channel axis can also take a curvilinear course, if this should be necessary for special applications.
  • cylinder section is meant the axial section of the pipetting channel along which the cylinder extends.
  • the tightness required to operate the working space may be established between the piston and cylinder by confronting a radially outer surface of the cylinder in a working cylinder end region near the working longitudinal end region of the piston-cylinder system with a radially inner surface of the piston one of the two surfaces is provided a seal which on the other surface is applied to seal the piston and the cylinder against each other and thus the working space from the environment.
  • the piston may have a depression, which, based on the channel axis and when viewed in the mounted state, is limited in the radial direction by a piston skirt circulating around the channel axis, is limited in the first axial direction by a piston crown, and is open in a second axial direction opposite to the first axial direction.
  • piston skirt for reasons of particularly simple production is advantageously cylindrical.
  • the piston if injection-molded, can be provided with draft angles so that the piston skirt tapers or widens from its open longitudinal end to the piston crown.
  • the piston-cylinder system can then be easily configured with outer piston when the working cylinder end portion is received in the recess of the piston such that the piston and cylinder are movable relative to each other.
  • such a pressure sensor is usually coupled via a lateral opening in the cylinder wall to the working space.
  • the pressure is merely a preferred state variable of the working fluid, which is particularly easily detectable and meaningful with regard to the quality of a dosing process.
  • the pipetting device it is advantageous for the pipetting device if the sensor is provided at the greatest possible distance from the actual location of the recording of the working fluid in the working space in order to avoid an interaction between the sensor and the dosing fluid as much as possible, in particular mutual contamination or malfunction of the sensor. Therefore, for monitoring the quality of a dosing process with a pipetting device according to a development of the present invention, it is contemplated that a sensor is received on the piston which is designed to detect at least one state variable of the working fluid, preferably its pressure.
  • the temperature and / or the density of the working fluid could be detected.
  • the outer bulb of the pipetting device according to the invention is formed with a depression, so that it can be provided for attachment of the sensor to the piston that a recess bounding the piston wall has an opening at which the sensor is provided for detecting the state variable.
  • a particularly functionally reliable and space-saving possibility for mounting the sensor on the piston is to close the opening in the piston wall with the sensor. Particularly preferred, because it saves a considerable amount of space, it is when the sensor forms part of a piston wall bounding the recess of the piston. In this case, not only the case is to be detected that the sensor forms an integral part of the piston wall, but also that case in which the sensor, optionally via a substrate or the like, is attached directly to the closing of the opening on the piston.
  • the senor can be arranged in the region of the piston crown, in particular form a part of the same.
  • the piston is formed at least in two parts, with a shell part which has at least one passage opening, and with a cover part which is connected to the shell part such that it closes the at least one passage opening on one side.
  • the shell part then forms with respect to the channel axis a radial wall of the piston-cylinder system formed using the piston, while the cover part forms an axially facing interface of the piston.
  • the senor can be arranged so as to save space so that it is traversed by the virtual channel axis.
  • At least one sensor for detecting a state variable of the working fluid can be provided on the cover part.
  • the sensor is arranged at a location which in the mounted state of the pipetting device is aligned axially with the passage opening in the casing part, ie is located radially within a boundary wall of the casing part for limiting the passage opening in an end region near the lid part.
  • a pipetting head with a plurality of working spaces, which are preferably arranged in the manner of a matrix.
  • a matrix is preferably a matrix applied in mutually orthogonal rows and columns.
  • the individual work spaces are usually formed separately from each other.
  • a quality check of dosing is particularly simple and thorough possible if each workspace is assigned a sensor for detecting a state variable of the working fluid.
  • the shell part can be designed as a perforated plate to form a plurality of outer pistons.
  • the thickness of the perforated plate can be oriented at the desired stroke of the outer bulb, so that the thickness of the shell part at least corresponds to the desired stroke of the outer bulb, optionally with the addition of safety distances for seals and collision avoidance.
  • a plurality of state-size sensors can be arranged on the cover part, preferably these are also arranged in a matrix, in accordance with the intended arrangement of outer bulb. It is particularly simple to arrange the state-size sensors on an end face of the cover part on the surface thereof, although it should not be ruled out that the cover part has depressions or even passage openings in which the state-size sensors are accommodated.
  • the at least one state variable sensor is connected via lines to a control and / or computer unit, which processes signals supplied by the state variable sensor.
  • FIG. 1 is an essential portion of a pipetting device according to the invention generally designated 10. It is a multi-pipetting head with 384 pipetting channels 12, which are arranged in an orthogonal matrix of 16 ⁇ 24 pipetting channels 12.
  • Each pipetting channel 12 runs along a channel axis K from a metering-side longitudinal end 14 of the pipetting device 10 to a working-side longitudinal end 16 thereof.
  • the starting point of the description of the pipetting device 10 according to the invention is a frame-fixed central support plate 18, on which metallic cylinders 20 are received via insulation elements 22 made of electrically insulating elastomer.
  • the cylinders 20, which are formed as a hollow cylinder, are thus stationary, ie machine frame fixed to the support plate 18 added.
  • the pipetting channels 12 are connected to a signal line connection 23, of which only one is shown for the sake of simplicity.
  • FIG. 1 located below the frame-fixed support plate 18 is a relative to this along the channel axes K movable Quetschplatte 24, which in a conventional manner, the coupling of in the FIGS. 1 to 4 not shown pipetting tips on coupling ends 26 of the pipetting device 10 allows.
  • a scraper plate 30 movable relative to the support plate 18 along the channel axes K ensures safe ejection of pipetting tips from the coupling ends 26 of the pipetting channels 12 and thus for decoupling of pipetting tips from the pipetting tips FIG. 1 illustrated multiple pipetting head.
  • the cylinders 20 are also surrounded by an elastomeric isolation element 32 in the crimp plate 24 for electrical isolation therefrom.
  • the isolation elements 32 are arranged in the Quetschplatte 24 with a radial distance from the cylinders 20 so as not to hinder a relative movement of the Quetschplatte 24 relative to the cylinders 20.
  • the pipetting channels 12 each have a crimp ring 34, which by an axial movement of the Quetschplatte 24 in the FIGS. 1 and 2 is compressed axially below mediation of the ferrules 28 and thereby radially expanded due to its transverse contraction properties, so that a pipette tip, depending on the configuration of their in the coupling state the squeezing ring 34 radially outwardly negative feedback geometry frictionally and / or positively at the coupling end 26 of the pipetting 12th can be held.
  • FIG. 1 provided on the support plate 18 relative to this longitudinal of the channel axes K movable a metering plate 36.
  • This metering plate 36 which is drivable relative to the carrier plate 18 in the direction of the channel axes K for movement via a partially shown movement drive mechanism 38, forms a plurality of outer pistons 40 which radially and axially externally surround a working cylinder longitudinal end 20a of the cylinder 20.
  • the outer pistons 40 formed by the metering plate 36 have a depression 42 into which the area of the working cylinder longitudinal end 20a of the cylinders 20 is received.
  • Each pipetting channel 12 thus comprises a working space 44, which is defined by the filled with working fluid volume of the recess 42 of the piston 40 and in the FIGS. 1 to 4 Not shown inner volume in the cavity of the cylinder 20 and thus at least until the metering cylinder end 20 b of the cylinder 20 is sufficient.
  • the working space still extends as far as the axial longitudinal ends of the pipetting channels 12, ie up to the openings of the coupling ends 26 and, in the case of coupled pipetting tips, even into the pipetting tips.
  • the portion of the working spaces 44 surrounded by the pistons 40 and the cylinders 20 is of particular interest.
  • the cylindrical inner wall of the piston 40 is formed by a cylindrical insulating member 46 from each other for the purpose of electrical insulation of pistons and cylinders.
  • the metering plate 36 is formed in several parts in the present example, namely by a shell part 54 which contains a plurality of through holes 56 and is thus configured as a perforated plate of predetermined thickness.
  • the shell part 54 is closed by a cover part 58 in a first direction E, wherein a sealing mat 60 with through-openings 62 is provided for sealing the working space 44 at the contact point between the cover part 58 and the shell part 56.
  • a pressure sensor 64 is arranged for each pipetting channel 12. These pressure sensors 64, of which in FIG. 4 only one is shown, close the through holes 62 of the sealing mat 60 and thus form part of the piston crown 66th
  • the pressure sensors 64 are connected via signal lines, not shown, to a computer unit for evaluating the signals supplied by the pressure sensors 64.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
EP08008525A 2008-05-06 2008-05-06 Dispositif de pipetage pour l'aspiration et la distribution d'un fluide de dosage Active EP2123359B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AT08008525T ATE511921T1 (de) 2008-05-06 2008-05-06 Pipettiervorrichung zur aspiration und dispensation eines dosierfluids
ES08008525T ES2364990T3 (es) 2008-05-06 2008-05-06 Dispositivo de pipetar para la aspiración y distribución de un fluido dosificado.
EP08008525A EP2123359B1 (fr) 2008-05-06 2008-05-06 Dispositif de pipetage pour l'aspiration et la distribution d'un fluide de dosage
JP2009109567A JP4855496B2 (ja) 2008-05-06 2009-04-28 測定流体の吸引および分注のためのピペット装置
US12/435,772 US8245586B2 (en) 2008-05-06 2009-05-05 Pipetting apparatus for aspiration and dispensation of a metering fluid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP08008525A EP2123359B1 (fr) 2008-05-06 2008-05-06 Dispositif de pipetage pour l'aspiration et la distribution d'un fluide de dosage

Publications (2)

Publication Number Publication Date
EP2123359A1 true EP2123359A1 (fr) 2009-11-25
EP2123359B1 EP2123359B1 (fr) 2011-06-08

Family

ID=39760862

Family Applications (1)

Application Number Title Priority Date Filing Date
EP08008525A Active EP2123359B1 (fr) 2008-05-06 2008-05-06 Dispositif de pipetage pour l'aspiration et la distribution d'un fluide de dosage

Country Status (5)

Country Link
US (1) US8245586B2 (fr)
EP (1) EP2123359B1 (fr)
JP (1) JP4855496B2 (fr)
AT (1) ATE511921T1 (fr)
ES (1) ES2364990T3 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120181446A1 (en) * 2009-07-23 2012-07-19 Trojan Technologies Cleaning apparatus, radiation source module and fluid treatment system

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2732367T3 (es) 2013-03-15 2019-11-22 Douglas Scient Llc Pipeteador de lavado
WO2014169012A1 (fr) * 2013-04-11 2014-10-16 Rarecyte, Inc. Dispositif, système et procédé pour sélectionner un analyte cible
KR102425126B1 (ko) * 2018-02-12 2022-07-29 한국전자통신연구원 바이오 반응 유체 제어장치, 바이오 반응 시스템 및 바이오 반응 유체 제어방법

Citations (7)

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Publication number Priority date Publication date Assignee Title
EP0111796A1 (fr) * 1982-12-20 1984-06-27 Medicorp Holding S.A. Ampoule-seringue
US6471670B1 (en) * 1998-10-05 2002-10-29 Karl Enrenfels Fibrin sealant applicator system
EP1412759A2 (fr) 2001-03-09 2004-04-28 Hamilton Bonaduz AG Procede et dispositif d'evaluation d'un processus de dosage de liquide
GB2426293A (en) * 2005-05-19 2006-11-22 Bosch Gmbh Robert Dispensing device
EP1745851A1 (fr) 2005-07-22 2007-01-24 Tecan Trading AG Procede, dispositif et programme pour la classification des liquides
GB2429199A (en) * 2004-06-22 2007-02-21 Bosch Gmbh Robert A dosing apparatus for use in generating combinatorial material libraries
EP1782856A1 (fr) * 2005-11-03 2007-05-09 Dentaco Dentalindustrie- und Marketing GmbH Applicateur pour un produit liquide

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JPS52110484A (en) * 1976-03-12 1977-09-16 Stanley Electric Co Ltd Wiring unit for automobile and manufacturing process thereof
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IT1174815B (it) * 1983-09-08 1987-07-01 Mg 2 Spa Macchina per il dosaggio di una quantita' prefissata di polvere all'interno di un contenitore
US4563907A (en) * 1983-10-31 1986-01-14 Micromedic Systems Inc. Direct reading automatic pipette
JP3442261B2 (ja) * 1997-07-03 2003-09-02 株式会社日立製作所 試薬分注装置
US6393898B1 (en) * 2000-05-25 2002-05-28 Symyx Technologies, Inc. High throughput viscometer and method of using same
US20050006410A1 (en) * 2001-06-29 2005-01-13 David Bach Precision fluid dispensing system
JP4036053B2 (ja) * 2002-07-31 2008-01-23 日本パルスモーター株式会社 分注装置等におけるシリンダユニット、およびその吸入・吐出量調整システム
JP2004108884A (ja) * 2002-09-17 2004-04-08 Jasco Corp 微流量ポンプ、及びそれを用いたフローインジェクション分析装置と高速液体クロマトグラフ
DE202006010293U1 (de) * 2005-07-22 2006-08-31 Tecan Trading Ag Pipettiergerät mit Computerprogrammprodukt zum Akzeptieren oder Verwerfen von pipettierten Flüssigkeitsproben
DE102007006076B4 (de) * 2007-02-02 2008-10-30 Brand Gmbh + Co Kg Mehrkanal-Pipettiervorrichtung

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0111796A1 (fr) * 1982-12-20 1984-06-27 Medicorp Holding S.A. Ampoule-seringue
US6471670B1 (en) * 1998-10-05 2002-10-29 Karl Enrenfels Fibrin sealant applicator system
EP1412759A2 (fr) 2001-03-09 2004-04-28 Hamilton Bonaduz AG Procede et dispositif d'evaluation d'un processus de dosage de liquide
GB2429199A (en) * 2004-06-22 2007-02-21 Bosch Gmbh Robert A dosing apparatus for use in generating combinatorial material libraries
GB2426293A (en) * 2005-05-19 2006-11-22 Bosch Gmbh Robert Dispensing device
EP1745851A1 (fr) 2005-07-22 2007-01-24 Tecan Trading AG Procede, dispositif et programme pour la classification des liquides
EP1782856A1 (fr) * 2005-11-03 2007-05-09 Dentaco Dentalindustrie- und Marketing GmbH Applicateur pour un produit liquide

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120181446A1 (en) * 2009-07-23 2012-07-19 Trojan Technologies Cleaning apparatus, radiation source module and fluid treatment system

Also Published As

Publication number Publication date
JP2009271068A (ja) 2009-11-19
ES2364990T3 (es) 2011-09-20
US20090277285A1 (en) 2009-11-12
ATE511921T1 (de) 2011-06-15
JP4855496B2 (ja) 2012-01-18
EP2123359B1 (fr) 2011-06-08
US8245586B2 (en) 2012-08-21

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