EP2068141A3 - Kit zur Kalibrierung eines Photolumineszenzmesssystems - Google Patents

Kit zur Kalibrierung eines Photolumineszenzmesssystems Download PDF

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Publication number
EP2068141A3
EP2068141A3 EP09153880.1A EP09153880A EP2068141A3 EP 2068141 A3 EP2068141 A3 EP 2068141A3 EP 09153880 A EP09153880 A EP 09153880A EP 2068141 A3 EP2068141 A3 EP 2068141A3
Authority
EP
European Patent Office
Prior art keywords
fluorescence
measuring system
standards
kit
fluorescence spectra
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP09153880.1A
Other languages
English (en)
French (fr)
Other versions
EP2068141B1 (de
EP2068141A2 (de
Inventor
Ute Resch-Genger
Bernhard Schönenberger
Dietmar Pfeifer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bam Bundesanstalt fur Materialforschung Und- Prue
Sigma Aldrich GmbH
Original Assignee
Sigma Aldrich GmbH
Bundesanstalt fuer Materialforschung und Pruefung BAM
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Aldrich GmbH, Bundesanstalt fuer Materialforschung und Pruefung BAM filed Critical Sigma Aldrich GmbH
Publication of EP2068141A2 publication Critical patent/EP2068141A2/de
Publication of EP2068141A3 publication Critical patent/EP2068141A3/de
Application granted granted Critical
Publication of EP2068141B1 publication Critical patent/EP2068141B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G01N21/278—Constitution of standards
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/108331—Preservative, buffer, anticoagulant or diluent

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Die Erfindung betrifft ein Verfahren und ein Kit zur Kalibrierung eines Photolumineszenzmesssystems, insbesondere eines Fluoreszenzmesssystems. Das Kit umfasst eine Mehrzahl von Fluoreszenzstandards i und deren korrigierte und zertifizierte Fluoreszenzspektren Ii(λ), wobei die Fluoreszenzstandards i so gewählt sind, dass ihre spektral korrigierten Fluoreszenzspektren Ii(λ) einen breiten Spektralbereich mit hoher Intensität abdecken. Die Standards zeichnen sich zudem durch hohe Halbwertsbreiten FWHMi ihrer Banden von zumindest 1400 cm-1 aus. Gemäß dem erfindungsgemäßen Verfahren werden Teilkorrekturfunktionen Fi(λ) durch Quotientenbildung aus gemessenen Fluoreszenzspektren Ji(λ) und den entsprechenden korrigierten Fluoreszenzspektren Ii(λ) gebildet und zu einer Gesamtkorrekturfunktion F(λ) für einen breiten Spektralbereich miteinander verknüpft. Hierfür erfolgt eine Berechnung von Verknüpfungsfaktoren αi durch statistische Mittelung aufeinander folgender Teilkorrekturfunktionen Fi(λ) lediglich über einen vorgegebenen, begrenzten Überlappungsbereich λi/i+1 ±ΔλOL um die gegenseitige Überschneidungswellenlänge λi/i+1.
EP09153880.1A 2004-09-10 2005-09-07 Kit, dessen Verwendung und Verfahren zur Kalibrierung eines Photolumineszenzmesssystems Expired - Lifetime EP2068141B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004044717A DE102004044717B8 (de) 2004-09-10 2004-09-10 Verfahren und Kit zur Kalibrierung eines Photolumineszenzmesssystems
EP05090255.0A EP1635164B1 (de) 2004-09-10 2005-09-07 Verfahren zur kalibrierung eines photolumineszenzmesssystems

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP05090255.0A Division-Into EP1635164B1 (de) 2004-09-10 2005-09-07 Verfahren zur kalibrierung eines photolumineszenzmesssystems
EP05090255.0A Division EP1635164B1 (de) 2004-09-10 2005-09-07 Verfahren zur kalibrierung eines photolumineszenzmesssystems

Publications (3)

Publication Number Publication Date
EP2068141A2 EP2068141A2 (de) 2009-06-10
EP2068141A3 true EP2068141A3 (de) 2017-12-27
EP2068141B1 EP2068141B1 (de) 2020-06-24

Family

ID=35456030

Family Applications (2)

Application Number Title Priority Date Filing Date
EP09153880.1A Expired - Lifetime EP2068141B1 (de) 2004-09-10 2005-09-07 Kit, dessen Verwendung und Verfahren zur Kalibrierung eines Photolumineszenzmesssystems
EP05090255.0A Expired - Lifetime EP1635164B1 (de) 2004-09-10 2005-09-07 Verfahren zur kalibrierung eines photolumineszenzmesssystems

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP05090255.0A Expired - Lifetime EP1635164B1 (de) 2004-09-10 2005-09-07 Verfahren zur kalibrierung eines photolumineszenzmesssystems

Country Status (5)

Country Link
US (2) US7713741B2 (de)
EP (2) EP2068141B1 (de)
DE (1) DE102004044717B8 (de)
DK (1) DK2068141T3 (de)
ES (1) ES2819022T3 (de)

Families Citing this family (18)

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Publication number Priority date Publication date Assignee Title
DE102004044717B8 (de) * 2004-09-10 2010-12-16 BAM Bundesanstalt für Materialforschung und -prüfung Verfahren und Kit zur Kalibrierung eines Photolumineszenzmesssystems
DE102005049365A1 (de) * 2005-03-18 2006-09-21 BAM Bundesanstalt für Materialforschung und -prüfung Kalibriereinrichtung und Farbstoffkit sowie ihre Verwendung zur Charakterisierung von Lumineszenzmesssystemen
DE102005049364B4 (de) 2005-03-18 2023-05-25 BAM Bundesanstalt für Materialforschung und -prüfung Multifunktionelle Kalibriereinrichtung und Kit sowie ihre Verwendungen zur Charakterisierung von Lumineszenzmesssystemen
DE102007038753A1 (de) 2007-08-16 2009-02-19 Giesecke & Devrient Gmbh Vorrichtung und Verfahren für die Kalibrierung eines Sensorsystems
US20090319195A1 (en) * 2008-06-20 2009-12-24 Hoots John E Method of monitoring and optimizing additive concentration in fuel ethanol
DE102008040513B4 (de) 2008-07-17 2010-08-26 BAM Bundesanstalt für Materialforschung und -prüfung Verwendung einer langwellig emittierenden Cyaninverbindung als NIR-Fluoreszenzstandard und Kit zur Kalibrierung von Photolumineszenzmesssystemen
EP2233913B1 (de) 2009-03-24 2017-07-19 BAM Bundesanstalt für Materialforschung und -prüfung Optischer Standard zur Kalibrierung und Charakterisierung von optischen Messeinrichtungen
DE102011016509A1 (de) 2011-04-08 2012-10-11 Giesecke & Devrient Gmbh Verfahren zur Prüfung von Wertdokumenten
DE102011002080B4 (de) * 2011-04-15 2016-05-04 Lre Medical Gmbh Vorrichtung und Verfahren zur Bestimmung der Konzentration von Fluorophoren in einer Probe
US8760645B2 (en) 2011-05-24 2014-06-24 Idexx Laboratories Inc. Method of normalizing a fluorescence analyzer
CN102637827B (zh) * 2012-02-10 2014-11-05 北京工商大学 具有光电化学活性的半菁衍生物和多金属氧酸盐自组装薄膜
CN103115878B (zh) * 2012-12-29 2016-08-03 聚光科技(杭州)股份有限公司 纠正光谱漂移的方法及应用
DE102013109789B3 (de) * 2013-09-06 2015-01-22 Bundesrepublik Deutschland, vertreten durch das Bundesministerium für Wirtschaft und Technologie, dieses vertreten durch den Präsidenten der BAM, Bundesanstalt für Materialforschung und -prüfung NIR-Kalibrierstandard zur spektralen und lumineszenzquantenoptischen Kalibrierung einer optischen Messvorrichtung im NIR-Bereich umfassend 800 bis 1300 nm
TN2017000314A1 (en) 2015-02-10 2019-01-16 Ecolab Usa Inc Corrosion inhibitors and kinetic hydrate inhibitors
WO2022174056A1 (en) * 2021-02-12 2022-08-18 Scintillon Institute For Biomedical And Bioenergy Research Excitation-emission matrix flow cytometry systems and uses thereof
WO2022256582A1 (en) 2021-06-04 2022-12-08 Idexx Laboratories Inc. Method for callibrating sensitivity of a photometer
CN113720833A (zh) * 2021-07-26 2021-11-30 杭州春来科技有限公司 铅检测方法和装置
CN118425118B (zh) * 2024-07-05 2024-09-20 深圳大学 一种干式快速诊断与数据集成系统

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Also Published As

Publication number Publication date
US20100219333A1 (en) 2010-09-02
DE102004044717B8 (de) 2010-12-16
US7947502B2 (en) 2011-05-24
DE102004044717B4 (de) 2010-08-26
EP1635164B1 (de) 2021-09-01
DK2068141T3 (da) 2020-09-21
US20060214112A1 (en) 2006-09-28
EP1635164A2 (de) 2006-03-15
EP2068141B1 (de) 2020-06-24
DE102004044717A1 (de) 2006-03-16
EP2068141A2 (de) 2009-06-10
EP1635164A3 (de) 2008-07-16
US7713741B2 (en) 2010-05-11
ES2819022T3 (es) 2021-04-14

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