EP1973661A1 - Cage de champ électrique et procédé de fonctionnement associé - Google Patents

Cage de champ électrique et procédé de fonctionnement associé

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Publication number
EP1973661A1
EP1973661A1 EP07702841A EP07702841A EP1973661A1 EP 1973661 A1 EP1973661 A1 EP 1973661A1 EP 07702841 A EP07702841 A EP 07702841A EP 07702841 A EP07702841 A EP 07702841A EP 1973661 A1 EP1973661 A1 EP 1973661A1
Authority
EP
European Patent Office
Prior art keywords
cage
field
particles
feldkafig
electrode
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP07702841A
Other languages
German (de)
English (en)
Inventor
Thomas Schnelle
Torsten Müller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PerkinElmer Cellular Technologies Germany GmbH
Original Assignee
Evotec Technologies GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evotec Technologies GmbH filed Critical Evotec Technologies GmbH
Publication of EP1973661A1 publication Critical patent/EP1973661A1/fr
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces

Definitions

  • the invention relates to an electric field cage and an associated operating method according to the preamble of the dependent claims.
  • the particles are captured centrally between the electrode planes, where, on the one hand, the capture forces are lowest and, on the other hand, the flow velocity in the channel and thus the deflecting forces are greatest.
  • a voltage increase in the control of the conventional Feldkafige leads to a desired Increase the fixation force.
  • this is associated with an undesirable increase in the heating of the fixed particles, in particular in physiological or more highly conductive media.
  • planar electrode arrangements A disadvantage of these planar electrode arrangements is the fact that the particles to be fixed are repelled at right angles to the electrode plane in the case of negative dielectrophoresis, so that these electrode arrangements alone are not suitable for fixing and holding particles.
  • the known planar electrode arrangements can be used as field cages, if an additional force is utilized, such as the gravitational force or the force generated by a laser tweezer.
  • the invention is therefore based on the object to provide a correspondingly improved field cage.
  • the invention comprises the general technical teaching that at least one of the cage electrodes surrounds the other cage electrode in an annular manner.
  • annular cage electrode is geometrically not limited to circular cage electrodes, but closes different shapes.
  • the annular cage electrodes may be polygonal, rectangular, elliptical or generally round.
  • the outer ring electrode surrounds an inner ring electrode.
  • these two ring electrodes surround a third, for example, circular electrode. Both arrangements are particularly well suited for the manipulation of particles by means of negative dielectrophoresis.
  • a ring electrode comprises on the one hand ring electrodes in the strict sense, which are not filled inside. On the other hand, this term also includes electrodes in which only the circumference is annular, while the electrodes are filled inside.
  • the invention comprises the general technical teaching to use a substantially planar electrode structure as a field cage instead of the known three-dimensional field cages described above.
  • planar field cage used in the context of the invention is preferably to be understood as meaning that the individual cage electrodes are arranged only on one side with respect to the particle to be fixed, whereas the particles to be fixed are fixed within the field cage in the case of the conventional three-dimensional field cages described above that the individual cage electrodes surround the fused particles on different sides.
  • the cage electrodes are thus preferably located on a substrate (ie a surface), which may be, for example, glass, plastic or silicon.
  • the sub strat with the cage electrodes may for example be arranged on an upper channel wall of the carrier flow channel or on a lower channel wall of the carrier flow channel.
  • the individual cage electrodes have a vertical electrode spacing which is smaller than the lateral electrode spacing, whereas the electrode spacing in the case of the conventional three-dimensional field cages described above is substantially greater.
  • the field cage according to the invention has exactly two cage electrodes, but the invention is not limited in terms of the number of cage electrodes to exactly two cage electrodes for the spatial fixation of the suspended particles. Rather, it is also possible, for example, for the field cage according to the invention to have three, four, six or eight cage electrodes or a different number of cage electrodes.
  • the individual cage electrodes of the field cage are preferably each planar and preferably aligned parallel to one another.
  • all the cage electrodes are arranged in a common electrode plane, so that the entire electrode arrangement is exactly planar.
  • the Käfigelekt ⁇ are clear, however, in two parallel and arranged in mutually offset planes.
  • this variant also can be in the scope of the invention as a planar electrode arrangement referred ⁇ , since the individual electrodes of the cage in power to fixie ⁇ particle are arranged only on one side with respect to the.
  • the vertical electrode spacing is preferably substantially smaller than the lateral electrode extent.
  • the inner annular Kafig- electrodes may optionally be disposed above or below the outer annular Kafigelektrode.
  • the annular cage electrodes can be arranged concentrically or eccentrically to one another within the scope of the invention, but a concentric arrangement of the cage electrodes is preferred.
  • the inner annular cage electrode encloses an opening in a channel wall of a carrier flow channel, whereby the suspended particles can enter or exit through this opening in the channel wall.
  • the suspended particles can be transferred, for example, into fluidic quiet zones (for example storage reservoirs) or into other channels.
  • At least one of the annular cage electrodes can be open on one side and / or to have a passivation layer on one side in order to weaken the electrode arrangement in a specific direction.
  • the use of passivation layers for weakening the field cage has the advantage that the relative weakening of the field barrier generated by the field cage can be controlled via the frequency of the field.
  • cell biologically used molecules such as lamin, serve as an insulating layer. This exploits the fact that the coupling of the field into the carrier solution over the given passivation layer is frequency- and medium-dependent. So takes the field docking into the carrier solution with the frequency and decreases with the ratio of the conductivities of the medium and passivation layer and the thickness of the passivation layer.
  • the field cage opens in directions of the passivation layers.
  • the Feild cage can do this at the same time if all passivations are the same.
  • different passivation layers are applied and the field cage is then deposited at these locations e.g. one after the other / selectively opens.
  • the inflow of another medium can be used as a switch.
  • This procedure can both fill the nDEP ring arrays
  • nDEP ring structures on an eg rectangular grid is initially filled with individual neurons.
  • the growth of the axons can be allowed / switched according to the predefined passivations.
  • the openings can also be realized via a laser by ablation of electrode material after the growth of the cells.
  • nDEP-Rmg arrays can be used for collecting and, if appropriate, subsequent cryopreservation of, in particular, particulate material from suspensions.
  • the individual Kafigelektroden are either the same or different shapes.
  • the Feldkafig invention has a certain snap point (minimum of the electric field in negative dielectrophoresis), in which the particles are spatially fixed, the snap point is either directly to a channel wall of Tragerstromkanals or spaced to the channel walls of Tragerstromkanals.
  • the close-to-wall fixation of the suspended particles offers the advantage that the flow velocity is significantly lower there than in the middle of the carrier flow channel, so that smaller holding forces are sufficient for the spatial fixation of the suspended particles.
  • the substrate is provided with a passivation layer, a biochemical coating and / or a nanolayer.
  • the biochemical coating of the substrate can, for example, modify the adhesion properties of the substrate for the particles to be fixed and / or set differentiation signals for the particles to be fixed.
  • the substrate with the Kafxgelektroden the Feldkafigs is not arranged on a channel wall of Tragerstromkanals, but the substrate passes through the Tragerstromkanal in the direction of flow in the middle m shape of a membrane, so that the substrate divides the Tragerstromkanal into two sub-channels. This is particularly advantageous if there is an opening in the substrate through which particles can pass from one partial channel into the other partial channel of the carrier flow channel.
  • the field cage according to the invention is preferably a dielectrophoretic field cage, wherein optionally positive dielectrophoresis or negative dielectrophoresis can be used in order to spatially fix the suspended particles.
  • the invention includes a variant with a plurality of Feldkafigen each having preferably two or three Kafigelektroden, wherein the individual Feldkafige each allow a spatial fixation of one or more suspended particles.
  • the individual field cells are arranged in a matrix-like manner in a plurality of columns and a plurality of rows, with the electrical control of the field cells being effected by a plurality of column control lines and a plurality of row control lines.
  • a common column control line for all Feldkafige the respective column is provided, wherein the column control line is connected at each electrode arrangement of the respective column in each case with the first Kafigelektrode.
  • a common row control line for all field cells of the respective row is provided for each row of the field cells, the row control line being connected to the second cage electrode for each electrode arrangement of the respective row.
  • one of the cage electrodes can optionally be controlled electrically separately or lie on an electrically floating potential.
  • the invention comprises not only the Feldkafig described above, but also a microfluidic system with such Feldkafig and a cell biology device with such a microfluidic system, such as a Zeilsortierer, a ZeIl-Screenmg device or the like.
  • the invention also encompasses the use of a microfluidic system according to the invention in such a cell biological device.
  • the invention also encompasses a microamplulator for manipulating suspended particles, wherein the micromorphulator according to the invention has a field cage according to the invention in order to fix the suspended particles.
  • the micromultiplier can be designed as dielectrophoretic forceps.
  • Suitable electrode materials besides metals and doped semiconductors are also conductive polymers, such as, for example, polyanilm, polypyrrole or polythiophene. Also advantageous is the use of laser-modiflzierbaren polymers, such as polybisalkylthioacetylene. In the laser direct writing process, electrodes can be written into a polymer chip in this way, which is advantageous in particular for prototype construction.
  • the invention also relates to a corresponding operating method for the above-described erfmdungs- according microfluidic system.
  • the field cage it is possible for the field cage to be controlled for the spatial fixation of the particles and for the subsequent release of the fixed particles with different frequencies.
  • the control for the spatial fixation of the suspended particles is preferably carried out with a frequency which is sufficiently large to form a trapping field.
  • the subsequent electrical control for releasing the fixed particles takes place with a smaller frequency, which is sufficiently small to open the catch field at least in the region of the opening or the passivation layer.
  • the above-described opening of the annular cage electrodes on one side can be achieved, for example, by irradiating the cage electrodes by a laser, so that electrode material is removed from the irradiated cage electrodes, whereby the desired opening is formed.
  • a preferably optical test can also be carried out as to whether or not particles are fixed in the individual field cages.
  • Such an occupancy test is particularly advantageous if the microfluidic system has numerous electrode arrangements for fixing particles.
  • the microfluidic system is first charged with particles until all field cages are coated with suspended particles. Subsequently, the loading phase can be terminated and it can be followed by further operating phases.
  • the occupancy test thus allows a time minimization of the loading phase with simultaneous full occupancy of all electrode cages.
  • a chemical gradient can be generated between the individual field cages by influencing the flow accordingly. For example, chemical additives with the carrier flow can be fed into the microfluidic system for this purpose, it being possible to vary the inflow of the additives in terms of time and / or space within the carrier flow.
  • the electrode assembly used for particle fixing can additionally be used for a further purpose.
  • the electrode arrangement can be electrically controlled in order to trigger a stimulus on the particle fixed therein and / or to carry out an electrical measurement (for example impedance).
  • the particles to be suspended are preferably biological cells.
  • the invention is not restricted to biological cells, but instead also makes it possible, for example, to fix cell aggregates or other particles.
  • FIG. 1A shows a preferred exemplary embodiment of a microfluidic system according to the invention with a field cage with two concentric annular cage electrodes attached to the lower channel wall of the carrier flow channel are and allow a spatial fixation of the suspended particles
  • FIG. 1B shows the field distribution in the field cage from FIG. 1A
  • FIG. 1D shows the field distribution in a double-ring-force field cage, in which the ring electrodes are opened in a cross-shaped manner
  • FIG. 2 shows an alternative exemplary embodiment, in which the field cage is arranged on the upper channel wall of the carrier flow channel
  • FIG. 3 shows a substrate which carries a field cage, wherein the substrate can be arranged in the carrier flow channel, for example in the middle of the channel, and allows a passage of the suspended particles,
  • FIG. 4 shows an alternative exemplary embodiment of such a substrate with another configuration of the field cage
  • FIG. 5A shows an alternative exemplary embodiment of a microfluidic system according to the invention with a field cage, the field cage consisting of two annular concentric cage electrodes on the lower channel wall, which have passivation layers on one side,
  • FIG. 5B shows a modification of the exemplary embodiment according to FIG. 5A, wherein the passivation layer cause weakening in four directions
  • FIG. 5C shows a modification of the exemplary embodiment according to FIG. 5A, wherein the passivation layers cause a weakening m in three directions,
  • FIG. 6 shows an alternative exemplary embodiment with a mat ⁇ xformigen arrangement of a plurality of Feldkafigen for particle fixation
  • FIG. 7 shows the operating method according to the invention in the form of a flowchart
  • FIG. 8 shows a dielectrophoretic forceps according to the invention
  • FIG. 9 shows a further exemplary embodiment of a dielectrophoretic forceps according to the invention.
  • FIG. 10A shows a further exemplary embodiment of a microfluidic system according to the invention with a field cage with three concentric annular cage electrodes, FIG.
  • FIG. 10B shows the field distribution in the field cage according to FIG. 10A
  • FIG. 11A shows a further exemplary embodiment of a microfluidic system with a flat counterelectrode
  • FIG. IIB the field distribution in the microfluidic
  • Figures 12A-12I different exemplary embodiments of inventive dungsgedorfkafigen.
  • FIG. 1A shows, in a simplified form, an exemplary embodiment of a microfluidic system according to the invention with a carrier flow channel 1, through which a carrier liquid with particles 2, 3 suspended therein flows in the X direction.
  • the carrier flow channel 1 in this case has a lower channel wall 4 and an upper channel wall 5, wherein on the lower channel wall 4 a Feldkafig 6 is arranged, which consists of two circular, concentric annular electrodes 7, 8, which can be controlled independently and a allow spatial fixing of the particle 3 in the current Tragerflus- stechnik by the Feldkafig 6 generates an electric capture field, which is shown in perspective in Figures IB and IC.
  • a Feldkafig 6 which consists of two circular, concentric annular electrodes 7, 8, which can be controlled independently and a allow spatial fixing of the particle 3 in the current Tragerflus- stechnik by the Feldkafig 6 generates an electric capture field, which is shown in perspective in Figures IB and IC.
  • the two ring electrodes 7, 8 are in this case arranged coplanar in a common electrode plane, so that the snap point is likewise located directly on the lower channel wall 4 in the common electrode plane.
  • This near-wall fixation of the particle 3 is advantageous because the flow velocity is smaller there than in the middle of the Tragerstromkanals 1, so that relatively low holding forces sufficient to fix the particles 3 to spatially. This in turn allows a relatively weak electrical actuation of the field cage 6, so that the fixed particle 3 is only slightly impaired by field effects.
  • the particles can 3 by Liche zusharmonic ⁇ forces (eg. Inertial forces and the gravitational force ⁇ g) are fixed at the bottom under lopping
  • FIGS. IB and IC show the field profile in the field box 6 according to FIG. 1A in a central vertical section through (FIG. 1B) and in a horizontal plane above the electrode structure (FIG. 1C).
  • FIG. 1D shows the field profile in a horizontal plane above the electrode structure for a modified field cage in which the annular cage electrodes 7, 8 are not open but open in a cruciform manner.
  • FIG. 2 corresponds largely to the exemplary embodiment described above and shown in FIG. 1, so that reference is made to FIG. 1 to avoid repetition, the same reference numerals being used for corresponding components.
  • a special feature of this exemplary embodiment is that the Feldkafig 6 is not arranged on the lower channel wall 4, but on the upper channel wall 5 of the Tragerstromkanals 1.
  • additional forces for example, inertia forces or the gravitational force g, the snap point can also be shifted from the channel wall into the solution.
  • FIG. 3 shows a simplified perspective view of a substrate 9 made of glass, plastic or silicon with the field cage 6, as already described above with reference to FIGS. 1 and 2.
  • the substrate 9 there is a cylindrical opening 10 through which the particles 2, 3 can pass from the one side of the substrate 9 to the other side of the substrate 9, as is illustrated schematically by the dashed arrow lines.
  • the substrate 9 thus acts as a partition and, for example in the microfluidic system according to FIG. 1, can be arranged as a membrane in the means of the carrier flow channel 1 and extend in the longitudinal direction of the carrier flow channel 1, so that the substrate 9 in the carrier flow channel 1 has two adjacent subchannels separates each other.
  • FIG. 4 shows an alternative exemplary embodiment of a substrate 9, which largely corresponds to the exemplary embodiment described above and in FIG. 3, so that reference is made to FIG. 3 in order to avoid repetition, the same reference numbers being used for corresponding parts in the following ,
  • the opening 10 in the substrate 9 tapers conically upwards, wherein the two ring electrodes 7, 8 are arranged in different electrode planes.
  • the two electrode planes are aligned parallel to one another and spaced apart from one another, as a result of which the snap point is lifted out of the electrode plane.
  • the field cage 6 can also be referred to herein as a planar electrode arrangement, since the individual cage electrodes, with reference to the particle to be fixed, are arranged only on one side.
  • the distance of the electrode planes may preferably be smaller than the lateral electrode extension, ie the electrode extension in the Y direction.
  • FIG. 5A shows a further exemplary embodiment of a microfluidic system according to the invention that largely corresponds to the embodiment described above and illustrated in FIG. 1, so that reference is made to FIG. 1 to avoid repetition, with the same reference numerals being used for corresponding parts become.
  • a special feature of this embodiment is that the two ring electrodes 7, 8 each have a passivation layer 11, 12 on the downstream side.
  • the passivation layers 11, 12 weaken the capture field generated by the field cage 6 in the region of the passivation layer 11 or 12.
  • a weakening in the respective direction can also be achieved by applying a passivation only on the inner ring or only on the outer ring.
  • Applications for the embodiments according to FIGS. 5B and 5C are, for example, neuron networks or triangular gratings.
  • FIG. 5B shows a corresponding exemplary embodiment with four weakening points
  • FIG. 5C shows a further exemplary embodiment with three weakening points.
  • Figure 6 shows an alternative embodiment of a microfluidic system with numerous arrayed field cages, each consisting of two concentrically ordered at ⁇ ring electrodes 13, 14th
  • the individual field cages are arranged in matrix form in four rows and four columns and are electrically driven by four column control lines 15 and four row control lines 16.
  • the individual column control lines 15 are in each case connected to the outer ring electrode 13 of all field cages of the respective column.
  • the individual row control lines 16 are each connected to the inner ring electrode 14. If, for example, all line control lines with signals of one phase and all column control lines are driven in opposite phase, particles can be fixed in all field cages. A single particle may then be released by grounding the corresponding row and column control lines.
  • FIG. 7 shows the operating method of a microfluidic system with the matrix-shaped electrode arrangement shown in FIG.
  • the operating method essentially consists of a charging phase 17, a consolidation phase 18, a growth / differentiation phase 19 and an investigation phase 20, which are described in detail below.
  • the FeId- cages are connected to fix the flushed cells and dielectrophoretically driven, starting with the downstream field cages.
  • biological cells are spatially fixed in the individual field cages.
  • An optical occupancy test of the Individual field cages and unfixed cells are ejected as soon as whole field cages are covered with biological cells.
  • the fixed cells then attach themselves.
  • the electric field can be reduced or completely switched off.
  • the field cages used for the spatial fixation of the cells are then electrically driven in a special way in order to structure the forming cell structure.
  • a chemical gradient can be generated between the individual feeder cells by correspondingly influencing the flow conditions.
  • This operating method can also be used to set up a defined neural network. ⁇
  • FIG. 8 shows a simplified illustration of a dielectrophoretic forceps 21 which can be used to remove suspended particles from a suspension fluid.
  • the forceps 21 At its distal end, the forceps 21 has a hemispherical tip, the two annular cage electrodes 22,
  • FIG. 9 shows an alternative exemplary embodiment of a forceps 21 according to the invention, which largely corresponds to the embodiment described above, so that reference is made to the above description to avoid repetition, the same reference numerals being used for corresponding components.
  • a special feature of this embodiment is that the forceps 21 at its distal end a recess
  • Figure 10A alternative embodiment of a microfluidic system is largely consistent with the embodiment described above and shown in Figure 1, so that reference is made to avoid repetition of the above description of Figure 1, wherein the same reference numerals are used for corresponding components.
  • the field cage 6 has three cage electrodes 7, 8, 26, wherein the outer cage electrodes 7, 8 can be electrically controlled independently of each other, as already described above.
  • the inner cage electrode 26 can optionally be at an electrically floating potential or can also be driven electrically, as indicated by the dashed line.
  • FIG. 10B shows the field distribution of the field cage 6 according to FIG. 10A in a central vertical section through the electrode structure, wherein the electrodes 7 and 8 are driven in phase opposition and the electrode 26 is grounded.
  • Figure IIA shows a simplified perspective view of a further embodiment of a microfluidic system according to the invention, which largely matches the microfluidic systems described above, so reference is made to the above description to avoid repetition, the same reference numerals being used for corresponding details hereinafter.
  • the upper channel wall 5 of the carrier flow channel 1 is formed here as a planar counter electrode.
  • the counterelectrode in this case consists of a transparent material in order to allow undisturbed optical observation through the upper channel wall 5.
  • the planar counterelectrode may be made of indium tin oxide (ITO) on the upper channel wall 5, but other materials are possible.
  • the field cage 6 is arranged on the lower channel wall 4 in this exemplary embodiment and thus lies opposite the planar counterelectrode on the upper channel wall 5.
  • FIG. IIB shows the field distribution E 2 in the microfluidic system according to FIG. IIA in the zy plane, which cuts the field cage 6 in the middle.
  • the central ring electrode 7 in this case has the same electrical potential as the counter electrode on the upper channel wall 5, while the outer ring electrode 8 is at the opposite electrical potential, which is achieved by a phase shift of 180 °.
  • the inner ring electrode 7 and the counter electrode 5 are grounded or are at a free potential, while the ring electrode 8 is driven with an alternating field.
  • FIGS 12A-12I show alternative embodiments of field cages according to the invention.
  • the two ring electrodes 7, 8 are each of square shape and are arranged with their edges parallel to one another.
  • the two ring electrodes I 1 8 are also each square shaped, but the ring electrode 7 is rotated relative to the ring electrode 8 by an angle of 45 °.
  • the ring electrode 8 is square, while the ring electrode 7 is hexagonally shaped.
  • the outer ring electrode 8 has the shape of an equilateral triangle.
  • the inner ring electrode 7 is circular or elliptical in these embodiments, wherein the figures 12D and 12E by a centric ( Figure 12D) and eccentric ( Figure 12E) arrangement of the inner
  • Ring electrode 7 differ within the outer ring electrode 8.
  • the two ring electrodes 7, 8 are each circular and concentric, wherein a triangular further electrode is arranged centrally within the inner ring electrode 7.
  • the outer ring electrode 8 the shape of a pentagon on, while the nere in ⁇ ring electrode 7 is arranged in a circle and centered within the outer annular electrode. 8
  • a further ring electrode is arranged within the inner ring ⁇ electrode 7, a further ring electrode is arranged.
  • the outer ring electrode 8 is star-shaped, while the inner ring electrode 7 is arranged in a circular centric manner within the outer ring electrode 8.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Electrochemistry (AREA)
  • Molecular Biology (AREA)
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  • Microelectronics & Electronic Packaging (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne une cage de champ (6) électrique pour la fixation tridimensionnelle de particules (2, 3) qui sont suspendues dans un fluide de support, en particulier dans un système microfluidique, avec plusieurs électrodes de cage (7, 8) commandés électriquement servant à produire un champ de capture. Il est prévu selon l'invention qu'au moins l'une des électrodes de cage (8) soit annulaire et qu'elle entoure l'autre électrode de cage (7). L'invention concerne en outre un procédé de fonctionnement associé.
EP07702841A 2006-01-18 2007-01-17 Cage de champ électrique et procédé de fonctionnement associé Withdrawn EP1973661A1 (fr)

Applications Claiming Priority (2)

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DE102006002462A1 (de) 2007-07-19
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