EP1958695A2 - Revêtement pour un agencement de récipients de réaction pour un mode de fonctionnement à une étape - Google Patents

Revêtement pour un agencement de récipients de réaction pour un mode de fonctionnement à une étape Download PDF

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Publication number
EP1958695A2
EP1958695A2 EP08002670A EP08002670A EP1958695A2 EP 1958695 A2 EP1958695 A2 EP 1958695A2 EP 08002670 A EP08002670 A EP 08002670A EP 08002670 A EP08002670 A EP 08002670A EP 1958695 A2 EP1958695 A2 EP 1958695A2
Authority
EP
European Patent Office
Prior art keywords
sample
covering
reaction vessel
movement
lid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP08002670A
Other languages
German (de)
English (en)
Other versions
EP1958695A3 (fr
Inventor
Arne Schafrinski
Henner Tasch
Lutz Timmann
Stefan Roth
Rüdiger HUHN
Thomas Uschkureit
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Publication of EP1958695A2 publication Critical patent/EP1958695A2/fr
Publication of EP1958695A3 publication Critical patent/EP1958695A3/fr
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/142—Preventing evaporation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T29/00—Metal working
    • Y10T29/49—Method of mechanical manufacture
    • Y10T29/49826—Assembling or joining

Definitions

  • the present invention relates to an apparatus and a method for carrying out processes and / or reactions which are carried out in a temperature-controlled environment.
  • the apparatus and method of the invention are not limited to this specific application, but rather relate to all applications known to those skilled in the art, in which a sample (s) / mixture (mixtures) in a particular Temperature to be edited.
  • the present invention relates to means for covering a sample or samples suitable for preventing or minimizing the evaporation and / or condensation of any vaporizable substance present in the sample (s) or reaction mixture (s). in particular the evaporation of substance at the edges of a vessel or an arrangement of vessels, the condensation of said substance on the lid of a reaction vessel or a plate / block containing the sample (s) and / or covering means ,
  • a thermal cycler for implementing chemical and / or biological reactions which comprises a housing for accommodating one or more reaction vessels and a cover, is used, for example, in US Pat EP 1 013 342 disclosed.
  • This device of a thermal cycler comprises a base housing for receiving one or more reaction vessels, which is open on the top, as well as a cover for completing this receiving area.
  • the apparatus also includes a locking mechanism for locking the cover in a predetermined position with respect to the reaction vessels and the base housing. If the cover Once closed, an electrically activated positioner is actuated so that a rigid portion of said cover is moved down against the reaction vessel (s).
  • thermocycler for the automated conduction of a polymerase chain reaction which has a closed temperature control system
  • US Pat U.S. 5,575,610 described in US Pat U.S. 5,575,610 described.
  • a structure having a slidable cover enclosing the sample tubes A heated plate is connected to a screw so that it can be moved up and down along a vertical axis to press this heated plate onto the reaction vessels with a defined pressure.
  • the application of a pressure in the z-direction is performed only after the slidable cover has been aligned with the reaction vessel (s).
  • the present invention also relates to a method for aligning and / or closing the cover of a device for controlling the temperature of at least one sample, said method comprising at least the following steps: (i) aligning and / or closing the covering means (3) in FIG a position in which the device is considered closed; (ii) transferring at least one lid (11, 12) of at least one covering means (3) from a position in which there is no physical and / or thermal contact with the at least one sample and / or at least one reaction vessel (1), in a position of physical and / or thermal contact with at least one reaction vessel (1) and / or a sample.
  • these two method steps are carried out simultaneously in a movement which moves both the covering means (3) and the cover (11, 12).
  • the means for positioning (24, 25) and the means for guiding (7) are adapted to one another such that a movement of the lid (11, 12) relative to the sample or the sample surface is effected, which within the same Time span has both a horizontal and a vertical component.
  • the lid (11, 12) preferably comprises a contact surface (12) and an inclined portion (11).
  • the lid comprises means for unrolling (14), preferably a circular belt.
  • both the closing of the means for covering (3) and the physical bringing into contact of the lid (11, 12) with at least one sample be effected by said rolling means, preferably by the movement of a circular belt.
  • the "horizontal" plane is the plane which is perpendicular to the direction of the gravitational direction and usually corresponds to the tabletop on which the device is mounted. In normal operation, this is also the plane defined by the arrangement of samples / reaction vessels to be covered. Accordingly, the vertical plane is along the direction of gravity and is perpendicular to the tabletop or plane defined by an array of reaction vessels.
  • the movement which simultaneously comprises a horizontal and a vertical component, is effected during the step of closing the covering means (3), in particular during the step aligning the covering means (3) with respect to the reaction vessel (1) and / or the receiving means (2) such that at the end of said movement the covering means (3), the at least one reaction vessel (1) and the Cover (11, 12) are in their respective end position.
  • the "end position” in the understanding of the present invention is the position in which the device is considered closed and the sample (s) can be processed, ie preferably the reaction (s) in the reaction vessel (in the reaction vessels ) can (can) be started.
  • the means for positioning (24, 25) and the means for guiding (7) are adapted to one another such that they have the means for covering (3) relative to the reaction vessel (s) (1) and / or or to or to the receiving means (2) so that the contact surface (12) of the lid is in physical and / or thermal contact with the at least one reaction vessel (1) and so that the means for covering (3) and the lid (11, 12) are in their predetermined end position, ie in the position in which the device is considered to be closed and the sample (s) can be processed, in which position preferably the reaction (s) in the reaction vessel (s) can be started ).
  • the movement described above is preferably a single continuous movement, more preferably a movement of the means for covering (3) relative to the means for receiving (2) and / or relative to the reaction vessel (s) (1) in the horizontal direction, that is, in a direction in which the covering means (3) moves in parallel relative to the picking means (2) and the reaction vessel (s) (1).
  • the movement of the means comprises Guide (7) and the contact area (12), which occurs simultaneously with the movement of the means for covering (3), preferably a horizontal component and a vertical component, ie the means for guiding (7) and the contact area (12) move not only parallel to the means for receiving (2) and / or the reaction vessel (1), but also, to a certain extent, in the vertical direction, ie perpendicular to the horizontal plane, as through the means for receiving and / or the reaction vessel (the reaction vessels) is defined.
  • the closure and alignment of the cover in its final position in a single continuous movement in accordance with the present invention has, among other things, the following advantages over the prior art embodiments: overall, comparatively simple movement is made to close the cover and provide close physical and / or or thermal contact between the lid (which typically has means for heating and / or cooling) with the at least one reaction vessel to effect. This lowers or minimizes the number of mechanical components needed to accomplish the task of aligning and closing the cover and contacting at least a portion of the cover in close physical and / or thermal contact with the sample (s) and / or sample Reaction vessel (the reaction vessels) to meet.
  • the pressure / force exerted by the contact area when closing the cover different types of reaction vessels can be accommodated within the same device, in particular reaction vessels having different heights and / or different height tolerances ,
  • the movement described above which simultaneously has a horizontal and a vertical component, can be performed manually and / or automatically (for example, by an electronically controlled actuator) or by any combination of manual and automated action.
  • the positioning means (24, 25) are realized as a rail (24) which is preferably part of a base housing (6) and / or realized as part of the receiving means (2) in connection with a corresponding movable rail element (25), which is preferably a part of the means for covering (3) or connected to these. It is further preferred that the end position of the means for covering (3) relative to the means for receiving (2) and / or relative to the sample and / or relative to the reaction vessel (to the reaction vessels) (1) determined by the rail element (25) is hit on the end of the path of the rail and / or the contact surface - or a part thereof - or on a stop device (26), which preferably coincides with the end of the travel of the rail (24).
  • the stop device (26) is a part of the base housing (6) and / or the means for receiving (2).
  • a “rail” in accordance with the present invention is any device that allows and / or allows the movement of the covering means (3) to be in a defined direction, preferably in the horizontal direction, such that the means for achieving Covering (3) preferably moving parallel to the plane defined by the reaction vessel (s) (1) and / or the receiving means (2).
  • the rail (24) and the movable rail element (25) are matched to one another such that movement of the covering means (3) relative to the picking means (2) and / or the sample (s) of the Reaction vessel (the reaction vessel) (1) is allowed, which is directed in one direction, preferably in the horizontal direction, ie that the means for covering (3) parallel to the means for receiving (2) and / or the basic housing ( 6) and / or the reaction vessel (reaction vessels) (1).
  • the movement of the means for guiding (7) is the movement of the means for guiding (7), which are preferably connected to the means for covering (3) and more preferably also to the lid (11, 12), indirectly with the movement of said means for covering ( 3) coupled.
  • indirectly coupled means that the means for guiding need not necessarily move in the vertical direction when the covering means move in the horizontal direction.
  • said indirect coupling is made possible by a sliding movement of the lid (11, 12), preferably mediated by an inclined portion of the lid (11).
  • This sliding movement is preferably effected by a parallel movement of the covering means (3) on the rail (24), while the lid (11, 12), in particular said inclined portion (11) of said lid, is in direct physical contact with the sample and / or the reaction vessel (s) (1) and / or the means for receiving (2).
  • the movement of the guiding means (7) is directly coupled to the movement of the covering means (3), preferably by a spring (9 ').
  • "direct coupling” refers to any effect of a vertical force on the guiding means (7) by moving the covering means (3) in the parallel direction.
  • the means for guiding (7) in particular with a component in the vertical direction to move without the need exists that the contact surface (12) in physical contact with the reaction vessel (s) (1) and / or the means for receiving (2) and / or the base housing (6).
  • the guiding means (7) there are no restrictions on the specific implementation of the guiding means (7) as long as said means for guiding permit movement of the lid (11, 12) which is not or not completely parallel (or perpendicular) to the lid horizontal plane, in particular not or not completely parallel (or perpendicular) to the plane defined by the sample and / or the reaction vessel (the reaction vessels) and / or the means for receiving (2) and / or the base housing (6) ,
  • said means for guiding (7) as at least one, preferably at least two units are realized, which are able to exert a lifting movement.
  • said unit for performing the lifting movement or said units for carrying out lifting movements are connected to both the covering means (3) and the lid (11, 12).
  • said lifting movement is exerted by a spring or by a pneumatic movement or by any other conceivable piston-like movement.
  • the pressure / force exerted by the lifting movement of the means for guiding (7) can be pre-adjusted and / or controlled, for example by adjusting the spring force and / or adjusting the travel distance of the rail element (25). on the rail (24).
  • the means for guiding (7) comprise at least one spring (9) which exerts pressure. More preferably, the pressure / force is predetermined to be sufficient to close the lid so that the contents of the vessel (s) are prevented from evaporating.
  • the lid (11, 12) is connected to the means for covering (3), preferably by means of guiding means (7) described above, which in turn are preferably also connected to the covering means (3).
  • the lid (11) comprises a contact surface (12) or consists of a contact surface (12), which is preferably deformable.
  • the movement of the cover (11, 12) is realized so that it has both a horizontal and a vertical component with respect to the plane passing through the base housing (6) and / or the sample surface and / or the reaction vessel (the reaction vessels) (1) and / or the means for recording (2) is defined.
  • This movement is in opposition to the movement of the covering means (3) as such, which preferably has only one parallel / horizontal component relative to the means for receiving (2) and / or the base housing (6) and / or the reaction vessel (1) ,
  • the lid (11, 12) comprises a material which is in direct contact with the reaction vessel (1) after alignment and / or closure of the covering means (3), i. in the final operating position, said material which is in direct contact with the reaction vessel (s) (1) being capable of chemically and / or physically reacting with the substances formed during the reaction (s) in the reaction vessel ( in the reaction vessels) (1), and / or which is sufficiently flexible to effect solid physical and / or thermal contact without destroying the reaction vessel (s) (1).
  • a (disponible) glass plate and / or a membrane and / or a liner is preferred, which can be attached to the lid (11, 12) and removed, if necessary.
  • the lid (11, 12) preferably has an inclined portion (11) and a contact surface (12) which is arranged parallel to the plane as defined by the reaction vessel (s) (1) and / or by the means for receiving (2). It is preferred that the angle of the inclined portion relative to the horizontal plane is chosen so that the forces exerted on the reaction vessel (s) are not strong enough to slide the reaction vessel (s) upon sliding of the contact surface , It is preferable that said contact surface (12) and said inclined portion of the lid (11) are integral with each other.
  • the circular movement of the circular belt is effected by guide rollers (13). This embodiment lowers or minimizes the friction between the contact surface and the reaction vessel (s).
  • the sample may be a single substance, a reaction mixture or any other conceivable material. Blank samples are included.
  • the at least one sample is located in at least one reaction vessel and / or in at least one well (s) of a plate, in particular a plate with wells for samples (multititer plate, PCR plate).
  • the sample may also be in a consumable / removable material placed on a flat block.
  • reaction vessel, plate or block may be removable or may be a permanent and / or integral part of the device, in particular the receiving means.
  • reaction vessels which optionally contain the at least one sample.
  • various types of reaction vessels can be used, and that even different reaction vessels can be used within the same set of experiments and / or can be contained in an array of the same recording means.
  • the present invention allows reaction vessels of different heights and / or different height tolerances to be used in combination.
  • the present invention not only ensures that locations in the plate or block or Reaction vessel holder may be empty (ie that they need not contain a reaction vessel), but also allows covering means, which are particularly advantageous for such an arrangement.
  • reaction vessels may be closed (i.e., they may have a lid or cover or may be covered by a glass plate or film or foil) or may be open. According to the invention, open reaction vessels can be used in addition to closed reaction vessels.
  • Preferred reaction vessels are reaction tubes known to those skilled in the art and suitable for carrying out PCR, including vessels having a flat bottom.
  • the reaction vessels, plates or blocks are sealed, for example by caps (which add an additional height of about 1 to 2 mm to the total height of the reaction vessels), in particular flat caps or domed caps or films / films (which may be one Thickness of about 0.02 mm).
  • these agents are capable of heating or cooling at least one sample and / or heating or cooling at least one reaction vessel or plate or block. It is preferred that the means for heating and / or cooling are selected from the group comprising resistance heating, fluid-mediated heating / cooling, cooling with air or gas, heating or cooling with Peltier elements, heating or cooling by friction (Joule) and / or heating by radiation.
  • At least one means for heating and / or cooling at least one sample and / or reaction vessel is part of the covering means.
  • said means for heating and / or cooling minimize or prevent the evaporation of a sample and / or minimize or avoid the condensation of the vaporized sample on or in the vicinity of the means for covering.
  • an (additional) means for heating and / or cooling in the means for receiving a plurality of samples and / or reaction vessels is provided.
  • means for heating and / or cooling are provided as part of a force distribution unit, in particular in connection with a medium or a material which deforms continuously under the action of a shearing force.
  • said medium or material is a fluid, in particular a gel or a liquid.
  • the gel or the liquid has a high thermal conductivity (at least 0.1 W ⁇ m -1 ⁇ K -1 ).
  • the liquid or gel is in contact with a heat exchange unit to change the temperature of the liquid / gel when in contact with the sample (s) and / or reaction vessel (s) Reaction vessels) is (are) brought.
  • a heat exchanger is a heating candle or a heating sheet.
  • means of taking at least one sample may be a holder for the reaction vessels or a block or plate, preferably a (flat) block made of metal, plastics or composite materials comprising wells or depressions or any other type of indentation / receptacle ,
  • the means for receiving may be, for example, a (microtiter) plate, a water bath with a reaction vessel holding insert, a carousel, any other type of multi-well plate, or a flat block.
  • the means for receiving are designed in the form of a block or a box. It is preferred that said agents are thermally isolated. It is further preferred that the means for receiving comprise means for heating and / or cooling the reaction vessel (s) and / or for heating the sample (s) from below and / or from the side.
  • the means for receiving may be disposable or reusable. They may be temporarily or permanently part of a basic housing or any other part of the device according to the invention.
  • the covering means are preferably temporarily or permanently attached to and / or aligned with the means for receiving the sample (s) or reaction vessel (s).
  • the means for covering and the means for receiving share a common base housing.
  • the unit comprising the means for receiving and the means for covering (optionally comprising a base housing ) as a whole completely enclose and / or enclose the at least one sample or a reaction vessel. Full encasement and / or encapsulation improves temperature stability.
  • the means for covering at least one sample or at least one reaction vessel are in physical and thermal contact with at least one force distribution unit, said force distribution unit being capable of bringing the at least one sample or the at least one reaction vessel into thermal contact with the at least one reaction vessel To provide means for covering and thereby causing (direct) thermal contact between the force distribution unit and the sample (the samples), so that effective heating and / or cooling of the at least one sample is ensured.
  • Strong mechanical and thermal contact between the sample and / or the reaction vessel, in particular in the upper region thereof and / or on the cover of the reaction vessel on the one hand, and the force distribution unit (which is part of the means for covering) on the other hand, are for a stable and effective thermal Treatment of the chemical or biological processes preferred.
  • a base housing (6) comprises means for receiving (2), which in this case receive a reaction vessel (1).
  • the base housing (6) also includes a rail (24) on which the Means for covering (3) can move freely in the horizontal direction [at least until a rail element (25) of the covering means reaches the end of the travel of the rail and / or hits a stop device (not shown)].
  • the means for covering (3) in FIG. 1 comprise two parallel guiding means (7) realized as holding supports directed downwards from the top cover of the covering means (3).
  • a unit comprising a lid (11, 12) hangs on said upper part of the covering means (3), the hanging being mediated by said guiding means (7).
  • the vertical component of the movement of the lid (11, 12) relative to the reaction vessel (1) is caused by pressure-exerting springs (9) and by a movable element of guiding means (7) capable of reciprocating movement in the direction to run down.
  • the unit comprising a cover (11, 12) also comprises heating and / or cooling means (4) for heating and / or cooling the upper part of the reaction vessel (1).
  • the contact surface (12) causes a uniform contact between the reaction vessel (1) and the means for heating and / or cooling in the final (operating) position.
  • FIG. 2 The embodiment which is in FIG. 2 is shown substantially corresponds to the embodiment, the FIG. 1 , with the additional feature of the means for unrolling (14), here realized as a circular belt, allowing the inclined portion (12) of the cover to slide on the reaction vessel (1).
  • the movement of the belt is accomplished by guide rollers (13).
  • FIG. 3 shows another preferred embodiment according to the present invention.
  • This includes funds for Covering (3) a movable rail member (25) moving in a rail (24) which is part of the base housing (6), the covering means (3) being connected to said rail member.
  • Said basic housing (6) holds the means for receiving (2), which in turn holds the reaction vessel (1).
  • the means for covering (3) slide from the right to the left side until the rail element (25) reaches the end of the travel of the rail (24) (see FIG FIG. 3B ).
  • the means for covering (3) comprise two means for guiding (7), which allow a lifting movement of the lid (12).
  • movement of the lid (12) in both the vertical and horizontal directions is achieved by a set of springs (9, 9 ') which guide the downward movement of the guide means which are connected to the contact surface are coupled with the sliding movement of the masking means (3) as illustrated by the sequence of movements that occur in the FIGS. 3A and 3B will be shown.
  • FIG. 3B the end position in which both the rail element (25) of the means for covering (3) on the end of the path of the rail (24) is taken, as well as the unit comprising the lid (12) in its end position with respect to the reaction vessel (1) (locked by hitting a stopper (26)).
  • the lid (12) comprising the means for heating and / or cooling (4) is in close physical and thermal contact with the reaction vessel (1) and a predetermined pressure is applied to said reaction vessel (1) by pressure Springs (9) of the means for guiding (7) exercised.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
EP08002670.1A 2007-02-13 2008-02-13 Revêtement pour un agencement de récipients de réaction pour un mode de fonctionnement à une étape Withdrawn EP1958695A3 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US88961607P 2007-02-13 2007-02-13

Publications (2)

Publication Number Publication Date
EP1958695A2 true EP1958695A2 (fr) 2008-08-20
EP1958695A3 EP1958695A3 (fr) 2013-07-03

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US (1) US20080318280A1 (fr)
EP (1) EP1958695A3 (fr)
CN (1) CN101288855B (fr)

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US5575610A (en) 1993-11-30 1996-11-19 Apic Yamada Corporation Transport-by-suction type die
EP1013342A2 (fr) 1998-12-22 2000-06-28 MWG -Biotech AG Dispositif de cyclage thermique
US6677151B2 (en) 2002-01-30 2004-01-13 Applera Corporation Device and method for thermal cycling
US6730883B2 (en) 2002-10-02 2004-05-04 Stratagene Flexible heating cover assembly for thermal cycling of samples of biological material

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202008009556U1 (de) * 2008-07-16 2009-12-03 Eppendorf Ag Vorrichtung zum Temperieren wenigstens einer Probe
DE102011011912A1 (de) * 2011-02-21 2012-08-23 Eppendorf Ag Pressanordnung für einen Deckel, insbesondere bei einem Laborgerät, und Verfahren zum Anpressen
DE102011011912B4 (de) * 2011-02-21 2020-09-03 Eppendorf Ag Laborgerät mit Deckel und Pressanordnung und Verfahren zum Anpressen

Also Published As

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US20080318280A1 (en) 2008-12-25
CN101288855A (zh) 2008-10-22
EP1958695A3 (fr) 2013-07-03
CN101288855B (zh) 2012-09-12

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