EP1013342A2 - Dispositif de cyclage thermique - Google Patents

Dispositif de cyclage thermique Download PDF

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Publication number
EP1013342A2
EP1013342A2 EP99125152A EP99125152A EP1013342A2 EP 1013342 A2 EP1013342 A2 EP 1013342A2 EP 99125152 A EP99125152 A EP 99125152A EP 99125152 A EP99125152 A EP 99125152A EP 1013342 A2 EP1013342 A2 EP 1013342A2
Authority
EP
European Patent Office
Prior art keywords
linear motor
cover
thermal cycler
base body
cycler device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP99125152A
Other languages
German (de)
English (en)
Other versions
EP1013342A3 (fr
EP1013342B1 (fr
Inventor
Wolfgang Dr. Heimberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
MWG Biotech AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Application filed by MWG Biotech AG filed Critical MWG Biotech AG
Publication of EP1013342A2 publication Critical patent/EP1013342A2/fr
Publication of EP1013342A3 publication Critical patent/EP1013342A3/fr
Application granted granted Critical
Publication of EP1013342B1 publication Critical patent/EP1013342B1/fr
Anticipated expiration legal-status Critical
Revoked legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Definitions

  • the invention relates to a thermal cycler device for carrying out chemical and biological reactions with a base body on which to receive a or a plurality of reaction vessels open at the top, a receiving area is formed is, a lid for closing the receiving area of the base body, and a spring element which is arranged such that the lid and the or Reaction vessels are pressed together, and the reaction vessels immediately be closed off by the lid or by means of an intermediate layer.
  • thermal cycler devices are known. They are called so-called stand-alone devices are used or integrated into automatic systems, to complete one or more, in particular molecular biological reactions semi-automatic.
  • Such an automatic system with an integrated thermal cycler device e.g. from US 5,443,791 forth.
  • a typical application for thermal cyclers is the PCR method.
  • a device for carrying out the PCR method is e.g. in US 5,656,493.
  • the spring element of the thermal cycler device mentioned at the outset serves to during the execution of a chemical and / or biological reaction in the Reaction tubes to close these with pressure, so that in the reaction tubes contained reagents and vapors cannot escape. In particular contamination of the reagents of a reaction vessel by the reagents of an adjacent reaction vessel can be safely avoided.
  • stronger spring elements which at closed lid a pressure of about 200 N on the reaction vessels or exercise on a rubber layer in between. This high force must be exercised when closing the lid. If the thermal cycler device as stand-alone device is used, so it is usually closed by hand, which is why the operator must exert this high force.
  • a thermal cycler device in which the cover consists of two parts, a cover body and one in the cover body movably arranged cover segment is formed. Between the The cover segment and the cover body have a mechanical clamping device arranged with a slip clutch, by means of the closed lid an additional force is exerted on the cover segment which acts against the in the reaction vessels arranged in a pipette plate are usually pressed.
  • This tensioning mechanism is the problem with opening and closing essentially eliminated.
  • the tensioning mechanism only a single certain pressure adjustable. When using different pipetting plates
  • the pressure should be individually adjustable, because pipetting plates with fewer reaction vessels require less pressure than pipetting plates with many reaction vessels.
  • the invention has for its object a thermal cycler device according to Develop the preamble of claim 1 such that they are easy to use is that an individually adjustable pressure on one in the thermal cycler device Reaction vessel or several reaction vessels can be exercised, and that it has a simple structure.
  • a spring element is arranged in the thermal cycler device such that the Lid can be pressed onto the reaction tube (s) and the reaction tubes directly from the lid or by means of an intermediate layer.
  • the thermal cycler device according to the invention is characterized by an electrical controllable actuator, which is arranged such that the cover and the reaction vessel or vessels with one compared to that generated by the spring Pressure increased pressure can be pressed together.
  • an electrically controllable actuator in particular an electrochemical Linear motor allows the necessary pressure regardless of Closing the thermal cycler.
  • an electrochemical linear motor is provided as electrically controllable
  • the actuator can adjust the pressure force by applying an appropriate current or Discharge of the linear electrochemical motor via an electrical resistor can be varied as required.
  • the provision of the electrochemical linear motor allows also a very simple embodiment of the thermal cycler device according to the invention, despite the large adjustment range for printing, no complicated ones mechanical elements such as Clutches, gears and the like necessary are.
  • electrochemical linear motors are e.g. in F&M, precision engineering, microtechnology, Microelectronics, magazine for electronics, optics and microsystem technology 105th year (1998), pages 527 - 530, in DE 43 31 764 C1, in DE 43 31 763 C1, described in DE 41 16 739 C1 and in DE 33 16 258 C2.
  • the electrochemical linear motor can be used both in the cover and in the base body the thermal cycler device according to the invention; is essential that by actuating the linear motor a compressive force on the interface between the one or more reaction vessels and the lid or one in between Liner is exercised.
  • the linear motor is only with one end on part of the thermal cycler device attached and the other end is freely movable, being a switch is provided such that a contact between the freely movable end of the linear motor and the part opposite it can be determined.
  • a switch is provided such that a contact between the freely movable end of the linear motor and the part opposite it can be determined.
  • the thermal cycler device 1 shown in the drawing for performing chemical and biological reactions has a base body serving housing 2, which is cuboid.
  • the upper bounding surface of the housing 2 is a receiving area 3 for receiving one Pipetting plate 4 formed.
  • the pipetting plate 4 is a thin-walled plastic part, in which a plurality of reaction vessels 5 are formed, arranged in rows and columns the pipetting plate are arranged.
  • a pipetting plate 24, 48 typically has or 96 reaction vessels.
  • the recording area 3 is provided with upwardly open, blind holes in which the respective reaction vessels 5 of the pipetting plate 4 are used.
  • the reaction vessels are preferably form-fitting to achieve good heat transfer on the blind hole-shaped recesses.
  • a heating and / or cooling element such as e.g. a Peltier element provided for heating and cooling the reaction vessels 5 of the pipetting plate 4 his.
  • the remaining area arranged below the receiving area 3 the housing 2 is in a known manner for receiving a power supply, an electrical control device and the like are provided.
  • a cover 6 Above the recording area 3, a cover 6 is arranged, which has a hinge joint 7 is pivotally attached to the housing 2.
  • a motor is preferably for pivoting the lid is provided around the hinge joint. Such a motor allows a fully automatic Opening and closing the lid.
  • the lid is formed from a lid base body 8 and a lid segment 9, the cover body 8 is a hollow cuboid body with four Side walls 10 and a top wall 11 is. At the bottom of the side walls 10, an inwardly projecting, circumferential web 12 is formed, which with its upper boundary surface 13 has a stop 12 for the cover segment 9 forms.
  • the lid segment 9 preferably forms a heating plate, which on a Temperature can be heated, which is slightly above the maximum reaction temperature lies that of the arranged in the receiving area 3 heating and / or Cooling element is generated.
  • the heating and / or cooling element can, for example, a temperature profile Drive in the range from 0 ° C to 95 ° C, then cover segment 9 is heated to 100 ° C, for example. This will cause condensation on the lid segment avoided.
  • the cover segment 9 is a substantially flat plate with an upper one Surface 14 and a lower surface 15 and a narrow circumferential Shell surface 16. At the upper edge of shell surface 16 there is one on the cover segment 9 circumferential bar 17 in front, which has a counter stop with its lower boundary surface 17 forms to stop 12 of the cover body 8.
  • the lid segment 9 is thus arranged displaceably in the cover base body 8 along its normal.
  • spring elements 18 are arranged, which counter the cover segment 9 press the stop 13 of the cover body 8.
  • the spring elements 18 are Coil springs, preferably four such spring elements 18 in the corner areas of the cover segment 9 are arranged. These spring elements 18 are, for example biased with a total spring force of about 20 N.
  • electrochemical linear motor 20 In the central area of the upper surface 14 of the cover segment 19, a flat recess 19 is made, in which an electrochemical linear motor 20 is arranged.
  • electrochemical linear motors are also referred to as electrochemical actuators, are described, for example, in F & M, Feinwerktechnik microtechnology, microelectronics, magazine for electronics, optics and microsystems, 1998, pages 527-530 and by the company FRIWO Silberkrafttechnik mbH, Meidericherstr . 6-8, D-47058 Duisburg available under the article name SK 5/300-AF.
  • This electrochemical linear motor has a bellows housing, which is sealed gas-tight and provided with two electrical leads, so that the following reversible electrochemical reaction can take place in a controlled manner in the bellows housing: Ni (OH) 2 ⁇ NiO (OH) + 1 ⁇ 2H 2
  • the electrochemical linear motor By supplying electricity, that is, by charging the electrochemical motor hydrogen is released in the bellows, causing the bellows to stretch and makes a linear movement. If the power supply is interrupted, so the bellows remains in its position. The bellows is powered by an electric Discharge resistance, the hydrogen gas is chemically bound again, which reduces the gas volume and contracts the bellows becomes.
  • the mechanical behavior of the electrochemical linear motor corresponds a pneumatic element, but no external compressed air supply needed, but can be controlled electrically, it being the three control states Loading, holding and unloading there.
  • the electrochemical linear motor 20 is at one end in the recess 19 of the Cover segment 9 attached, while its opposite end 21 freely movable is.
  • the electrochemical linear motor is actuated, i.e. when stretched, the electrochemical linear motor 20 is supported on the ceiling wall 11 of the cover body 8 and thereby pushes the cover segment 9 away from the top wall 11, that is to say when the thermal cycler device is closed below towards the receiving area 3 of the housing 2.
  • the thermal cycler device 1 preferably has the Ceiling wall 11 of the cover body 8 at the freely movable end 21 of the linear motor 20 opposite area on a switch 22 with which a Contact of the freely movable end 21 with the top wall 11 of the base body 8 determined and converted into an electrical signal.
  • the Switch 22 is, for example, a microswitch.
  • the switch 22 is connected to an electrical control device, which is the linear motor 20 controls.
  • Closing element 23 On the side of the cover 6 opposite the hinge joint 7 is a Closing element 23 is provided, which in a corresponding closing recess 24th can engage on the housing 2 and fix the cover 6 on the housing 2.
  • This Rubber mat 25 can be replaced together with the respective pipetting plates 4 be cleaned or between two incubation processes.
  • the thermal cycler device according to the invention without such Operate rubber mat or such a rubber layer permanently on the lower Attach surface of the lid segment 9.
  • the linear motor 20 is actuated, that is to say that current is supplied to the linear motor so that it extends.
  • the control device detects by means of the microswitch 22 the point in time t contact at which the linear motor 20 with its freely movable end 21 contacts the top wall 11 of the base body 8. From this point in time of contact, the linear motor 20 is supplied with a certain amount of electrical charge, which is determined by the product (I ⁇ t) of current I and time t. This amount of charge is proportional to the force exerted by the linear motor 20, so that the force with which the linear motor 20 presses the cover segment 9 in the direction of the receiving region 3 in addition to the spring elements 18 can be precisely determined.
  • the force exerted by the electrochemical linear motor 20 is typically in the range of approximately 200 N and can preferably be up to 300 N. Linear motors with a maximum force of 150 N to 800 N are expedient for the invention.
  • the force exerted by the linear motor 20 can be varied by varying the amount of charge supplied can be changed at will. This force is therefore arbitrary to the respective one Customizable requirements, with pipette plates with fewer tubes a lower force and for pipetting plates with more or larger reaction vessels a greater force is appropriate.
  • the force exerted by the linear motor 20 is regardless of the thickness of the rubber mat 25 or the thickness of the pipetting plates 4, since the force is only built up when the freely movable end 21 with the ceiling wall 11 of the lid is in contact and the amount of charge supplied from this point in time can be determined exactly.
  • the switch 22 can also be omitted, since then the distance a between the ceiling wall 11 and the freely movable end 21 of the fully retracted linear motor 20 is always the same and therefore the Linear motor 20 electric charge to be supplied until the freely movable end 21st touches the top wall 11, is always the same.
  • the force exerted by the linear motor 20 is proportional to the total electrical charge supplied to the linear motor 20 minus the to necessary for contact between the linear motor 20 and the ceiling wall, but constant electrical charge.
  • the switch 22 is on the cover segment 9 arranged and the electrochemical linear motor 20 on the top wall 11 attached.
  • the switch 22 can be used as an electrically conductive, for example annular contact field be formed, the housing of the electrochemical as a counter-contact Linear motor 20 functions.
  • the corresponding control circuit is thus over Housing of the linear motor passed, with a contact between the housing the linear motor and the contact field the start of the force build-up is determined.
  • a rubber bellows arranged so that a narrow cavity between the housing of the linear motor and the rubber bellows is formed. Furthermore, a Fan provided, the cooling air for cooling the linear motor in this space blows. This can extend the life of the linear motor.
  • spacer plate can be used here can be exchanged with different strengths, making the stroke different thick microtiter plates can be adapted.
  • the spacer plates are preferably attachable to the cover segment 9 with a quick-release fastener.
  • the thermal cycler device has a main heating device in the base body 2 and a cooling device and in the cover 6 a cover heating device on.
  • the lid heater is used to prevent condensation to a value preset by the user, for example in the range from 70 ° C to 120 ° C. This is preferred in comparison to the main heating device much slower lid heater simultaneously with the actuation of Linear motor 10 started.
  • the main heater starts the heating process, when the lid heater either reaches the set end temperature or a threshold of e.g. Has exceeded 85 ° C.
  • the housing in two parts with a housing base and a housing segment on which the receiving area is designed for the reaction vessels. Between the housing segment and the housing body can then the spring elements and the electrochemical Linear motor can be arranged to move the receiving area upwards to press to the lid. It is essential for the invention that the majority of Pressure between the reaction vessels and the lid not during the closing process of the lid must be applied, but after closing the lid is generated by the linear motor and dismantled before opening the cover becomes. This makes the lid as easy as with conventional thermal cycler devices with significantly less pressure between the reaction vessels and the lid are handled. Snap open the lid due to a considerable bias due to the inventive design safely avoided. At the same time, the pressure is over a wide range freely variable and precisely dosed. These benefits come with a very inexpensive and simple design achieved.
  • Such one Actuator can e.g. one driven by an electric motor and a spindle Toggle lever, an eccentric driven by an electric motor or one by one Electric motor driven wedge mechanism.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Confectionery (AREA)
  • Heating, Cooling, Or Curing Plastics Or The Like In General (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Thermally Actuated Switches (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Glass Compositions (AREA)
EP99125152A 1998-12-22 1999-12-16 Dispositif de cyclage thermique Revoked EP1013342B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19859586 1998-12-22
DE19859586A DE19859586C1 (de) 1998-12-22 1998-12-22 Thermocyclervorrichtung

Publications (3)

Publication Number Publication Date
EP1013342A2 true EP1013342A2 (fr) 2000-06-28
EP1013342A3 EP1013342A3 (fr) 2001-01-10
EP1013342B1 EP1013342B1 (fr) 2004-04-07

Family

ID=7892332

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99125152A Revoked EP1013342B1 (fr) 1998-12-22 1999-12-16 Dispositif de cyclage thermique

Country Status (7)

Country Link
US (1) US6153426A (fr)
EP (1) EP1013342B1 (fr)
JP (1) JP4578604B2 (fr)
AT (1) ATE263628T1 (fr)
AU (1) AU730946B2 (fr)
DE (2) DE19859586C1 (fr)
NO (1) NO996187L (fr)

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WO2002026384A2 (fr) * 2000-09-29 2002-04-04 Promega Corporation Plaque d'essai a plusieurs cupules et porte-plaque, et procede de montage de la plaque
DE20117661U1 (de) * 2001-10-29 2003-03-13 Mwg Biotech Ag Thermocyclervorrichtung
EP1958695A2 (fr) 2007-02-13 2008-08-20 Eppendorf AG Revêtement pour un agencement de récipients de réaction pour un mode de fonctionnement à une étape
EP1961484A1 (fr) * 2007-02-13 2008-08-27 Eppendorf Ag Couvercle pour échantillons avec une application de pression homogène
EP1964609A1 (fr) * 2007-02-13 2008-09-03 Eppendorf Ag Couvercle pour échantillons avec un réglage de hauteur indépendant de la taille des échantillons
WO2009102924A1 (fr) 2008-02-15 2009-08-20 Bio-Rad Laboratories, Inc. Cycleur thermique comportant un couvercle à réglage automatique
DE202008009556U1 (de) 2008-07-16 2009-12-03 Eppendorf Ag Vorrichtung zum Temperieren wenigstens einer Probe
DE102010019232A1 (de) * 2010-05-03 2011-11-03 Eppendorf Ag Kondensatvermeidungshaube
DE102011011912A1 (de) * 2011-02-21 2012-08-23 Eppendorf Ag Pressanordnung für einen Deckel, insbesondere bei einem Laborgerät, und Verfahren zum Anpressen
EP3632568A1 (fr) 2018-10-02 2020-04-08 Biometra GmbH Dispositif de traitement thermique des échantillons
DE102018131127A1 (de) * 2018-12-06 2020-06-10 Analytik Jena Ag Automatisierbare Temperiervorrichtung

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US20040191831A1 (en) * 2003-03-25 2004-09-30 Council Of Scientific And Industrial Research Rapid heat - mediated method for enzyme - linked immunosorbent assay procedure
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EP1758981A4 (fr) 2004-05-28 2013-01-16 Wafergen Inc Appareil et procedes pour effectuer des analyses multiplex
US20050282270A1 (en) * 2004-06-21 2005-12-22 Applera Corporation System for thermally cycling biological samples with heated lid and pneumatic actuator
US20060024204A1 (en) * 2004-08-02 2006-02-02 Oldenburg Kevin R Well plate sealing apparatus and method
WO2006012893A1 (fr) * 2004-08-05 2006-02-09 Jpk Instruments Ag Dispositif pour recevoir un echantillon d'essai
EP2109627A4 (fr) 2007-01-22 2014-07-23 Wafergen Inc Appareil permettant la mise en oeuvre de réactions chimiques à haut rendement
US8865457B2 (en) * 2007-03-15 2014-10-21 Siemens Healthcare Diagnostics Inc. Active, micro-well thermal control subsystem
CN101715429B (zh) * 2007-05-15 2012-05-23 和光纯药工业株式会社 均衡集成式pcr-ce微流装置内的压力的压力歧管
US20110156090A1 (en) * 2008-03-25 2011-06-30 Lin Charles W C Semiconductor chip assembly with post/base/post heat spreader and asymmetric posts
WO2010115160A2 (fr) * 2009-04-03 2010-10-07 Helixis, Inc. Dispositifs et procédés pour chauffer des échantillons biologiques
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GB201018624D0 (en) 2010-11-04 2010-12-22 Epistem Ltd Reaction vessel
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WO2014149268A1 (fr) 2013-03-19 2014-09-25 Life Technologies Corporation Couvercle de thermocycleur
EP2992377B1 (fr) 2013-05-01 2021-01-06 Bio-Rad Laboratories, Inc. Affichage de microscope numérique réglable
US20140327349A1 (en) * 2013-05-02 2014-11-06 Bio-Rad Laboratories, Inc. Drawer Handle Mechanism
GB2526520B (en) * 2014-04-04 2021-08-18 It Is Int Ltd Biochemical reaction system
EP3253492B1 (fr) * 2015-02-06 2024-04-03 Life Technologies Corporation Systèmes d'analyse biologique
US10641772B2 (en) 2015-02-20 2020-05-05 Takara Bio Usa, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
US20180264476A1 (en) * 2015-09-16 2018-09-20 Fluoresentric, Inc. Apparatus, systems and methods for dynamic flux amplification of samples
WO2017098321A1 (fr) * 2015-12-11 2017-06-15 Spartan Bioscience Inc. Système et procédés de fermeture étanche de tubes pour l'amplification d'acide nucléique
US11460405B2 (en) 2016-07-21 2022-10-04 Takara Bio Usa, Inc. Multi-Z imaging and dispensing with multi-well devices
CN110157590A (zh) * 2018-02-13 2019-08-23 光鼎生物科技（江苏）有限公司 热循环仪
DE102018124412A1 (de) * 2018-10-02 2020-04-02 Biometra GmbH Temperierblockmodul und Vorrichtung zur thermischen Behandlung von Proben
JP7327360B2 (ja) * 2020-11-19 2023-08-16 横河電機株式会社 熱処理システム、核酸抽出システム、核酸分析システム

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WO2001037997A1 (fr) * 1999-11-23 2001-05-31 Glaxo Group Limited Appareil destine au chauffage et au refroidissement de microplaques pharmaceutiques a puits profonds
WO2002026384A2 (fr) * 2000-09-29 2002-04-04 Promega Corporation Plaque d'essai a plusieurs cupules et porte-plaque, et procede de montage de la plaque
WO2002026384A3 (fr) * 2000-09-29 2002-08-15 Promega Corp Plaque d'essai a plusieurs cupules et porte-plaque, et procede de montage de la plaque
DE20117661U1 (de) * 2001-10-29 2003-03-13 Mwg Biotech Ag Thermocyclervorrichtung
US8492137B2 (en) 2007-02-13 2013-07-23 Eppendorf Ag Cover for sample with homogenous pressure application
US9289769B2 (en) 2007-02-13 2016-03-22 Eppendorf Ag Cover for sample with sample-size independent height adjustment
EP1964609A1 (fr) * 2007-02-13 2008-09-03 Eppendorf Ag Couvercle pour échantillons avec un réglage de hauteur indépendant de la taille des échantillons
EP1961484A1 (fr) * 2007-02-13 2008-08-27 Eppendorf Ag Couvercle pour échantillons avec une application de pression homogène
EP1958695A2 (fr) 2007-02-13 2008-08-20 Eppendorf AG Revêtement pour un agencement de récipients de réaction pour un mode de fonctionnement à une étape
AU2008200653B2 (en) * 2007-02-13 2012-12-06 Eppendorf Ag Cover for sample with sample-size independent height adjustment
EP2359933A1 (fr) 2007-02-13 2011-08-24 Eppendorf AG Couvercle pour échantillons avec un réglage de hauteur indépendant de la taille des échantillons
EP2364777A1 (fr) 2007-02-13 2011-09-14 Eppendorf AG Couvercle pour échantillons avec une application de pression homogène
CN101439306B (zh) * 2007-02-13 2012-07-04 埃佩多夫股份公司 用于样品尺寸独立于高度调节的样品的盖
EP2247714A4 (fr) * 2008-02-15 2011-12-28 Bio Rad Laboratories Cycleur thermique comportant un couvercle à réglage automatique
EP2247714A1 (fr) * 2008-02-15 2010-11-10 Bio-Rad Laboratories, Inc. Cycleur thermique comportant un couvercle à réglage automatique
US8784753B2 (en) 2008-02-15 2014-07-22 Bio-Rad Laboratories, Inc. Thermal cycler with self-adjusting lid
WO2009102924A1 (fr) 2008-02-15 2009-08-20 Bio-Rad Laboratories, Inc. Cycleur thermique comportant un couvercle à réglage automatique
DE202008009556U1 (de) 2008-07-16 2009-12-03 Eppendorf Ag Vorrichtung zum Temperieren wenigstens einer Probe
DE102010019232A1 (de) * 2010-05-03 2011-11-03 Eppendorf Ag Kondensatvermeidungshaube
DE102010019232B4 (de) * 2010-05-03 2013-06-27 Eppendorf Ag Kondensatvermeidungshaube
DE102011011912A1 (de) * 2011-02-21 2012-08-23 Eppendorf Ag Pressanordnung für einen Deckel, insbesondere bei einem Laborgerät, und Verfahren zum Anpressen
DE102011011912B4 (de) * 2011-02-21 2020-09-03 Eppendorf Ag Laborgerät mit Deckel und Pressanordnung und Verfahren zum Anpressen
EP3632568A1 (fr) 2018-10-02 2020-04-08 Biometra GmbH Dispositif de traitement thermique des échantillons
US11247211B2 (en) 2018-10-02 2022-02-15 Biometra GmbH Apparatus for the thermal treatment of samples
DE102018131127A1 (de) * 2018-12-06 2020-06-10 Analytik Jena Ag Automatisierbare Temperiervorrichtung

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Publication number Publication date
DE19859586C1 (de) 2000-07-13
EP1013342A3 (fr) 2001-01-10
ATE263628T1 (de) 2004-04-15
DE59909106D1 (de) 2004-05-13
AU6542799A (en) 2000-06-29
AU730946B2 (en) 2001-03-22
NO996187L (no) 2000-06-23
US6153426A (en) 2000-11-28
JP2000189152A (ja) 2000-07-11
NO996187D0 (no) 1999-12-14
JP4578604B2 (ja) 2010-11-10
EP1013342B1 (fr) 2004-04-07

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