EP1901757A2

EP1901757A2 - Preparations made from natural materials

Info

Publication number: EP1901757A2
Application number: EP06792472A
Authority: EP
Inventors: Wolf-Dieter Juelich; Ulrike Lindequist; Sabine Witt; Joern Kasbohm; Gerold Lukowski; Kristian Wende; Hans-Peter Welzel; Winfrid Ruehle; Frank Szrama; Martina Wurster; Sebastian Lippemeier; Joachim Schomburg; Christian Schultz; Peter Hartig; Ralf Hintze
Original assignee: Ernst Moritz Arndt Universitaet Greifswald
Current assignee: PATENTHANDEL PORTFOLIOFONDS I GMBH & CO. KG; Universitaet Greifswald
Priority date: 2005-06-30
Filing date: 2006-06-30
Publication date: 2008-03-26
Also published as: WO2007003613A2; WO2007003613A3

Abstract

The invention relates to compositions made from biomasses and/or extracts of at least one terrestrial fungus, preferably a type of the species Ganoderma and at least one sheet silicate, a iron-rich ore, biomasses obtained from microalgae, fungal biopolymer complexes or mineral mixtures. The compositions are particularly suitable as UV-filter agents made from natural materials, preventing cell aging and having an anti-aging effect. Biomasses are preferably applied in micro- and nano-particulate form. Further applications are as food supplements, plant promoters, free radical protection or medicaments.

Description

Preparations based on natural substances

description

The invention relates to compositions of biomass and / or extracts of at least one terrestrial fungus, preferably a species of the genus Ganoderma on the one hand and at least one layered silicate, an iron-rich raw material, biomass, obtainable from microalgae, fungal biopolymer complexes on the other. The compositions prevent premature cell aging and have an anti-aging effect. Possible applications include food and feed supplements, plant tonics or drugs that are designed to protect against free radicals. They are also suitable as UV protection agents based on natural substances.

Prior art I. Food and feed supplements

Dietary supplements with extracts of G. lucidum can be successfully used for the supportive treatment of immunological disorders, cancer, AIDS, hepatitis, diabetes and cardiovascular diseases In the cited reference, the interaction of many effects and factors is the basis for prophylaxis of these diseases and thus for an anti-aging effect (Pei gene Xiao et al., J. Ethnopharmacology 1993).

A water extract or a low alcohol extract of G. lucidum can be used as a cosmetic (WO 01/32192 Al). An alcoholic extract of G. lucidum can be used in dermatoses to prevent inflammation (JP 1180889 A).

The anti-oxidative properties of Ganoderma lucidum are known (Mau JL; Lin HC; Chen CC: Antioxidant properties of several medicinal mushrooms J Agric Food Chem 2002 Oct 9; 50 (21): 6072-7; You YH; Lin ZB: Protective effects of Ganoderma lucidum Polysaccharides peptide on injury of macrophages induced by reactive oxygen species Acta Pharmacol Sin 2002 Sep; 23 (9): 787-91). Bioactive substances with radical scavenger capacity can also be extracted from the spores of G. lucidum. The use of radical scavenging properties of G. lucidum as a single substance is difficult (British J. Nutriation (2004). In order to enhance the antioxidant effect, it is possible to mix G. lucidum with grapefruit seed extracts (WO 02/11744 Al). It is also possible to enrich G. lucidum during cultivation with selenium (WO 01/05938 Al). Also known are mixtures with the botanical antioxidants green tea extract, anthocyanins from bilberry fruits, ginkgo, guarana root extract (PCT WO 2004/100896 A2).

An advantageous use of the antioxidant properties results in the andropause in men (US2003 / 0054014 Al). It is the state of the art to prepare extracts of G. lucidum by treatment with solvents. It is also in the art to recover extracts of G. lucidum by treatment with supercritical CO ₂ (WO 98/13512 A1).

The description of the anti-oxidative properties has hitherto focused exclusively on Ganoderma lucidum.

From WO 00/53207 Al or US 6,726,911 Bl new antimicrobial compounds of fungi of the species Ganoderma pfeifferi are known. From the fruiting body and the mycelium of the species Ganoderma pfeifferi extracts are available that are effective against bacteria and viruses and are suitable as preservatives for pharmaceutical and cosmetic preparations and for combating infections. Also in methanolic extracts of commercial fungi antioxidant properties were detected with the DPPH test (Food chemistry (2002)).

Another way to supplement food or feed is taken when free radicals are removed by adsorption from the organism. It uses the microporous structure of certain minerals. For example, zeolite crystals have tiny cavities and channels, which is due to their ability to absorb free radicals. In this case, other harmful substances are removed. For example, the mineral clinoptiolite is a component of the product Phytosorb, which is used as a toxin binder for pig and cattle feed. The animal feed additive Lucoton-T uses the absorption capacity of chitin and melanin. For a high absorption ability also phyllosilicates are known. Phyllosilicates are salts of silicic acid in which the [SiO ₄ ] tetrahedra are interlinked in one plane and thus form lattice lattices. Some of the silicon atoms are replaced by magnesium or aluminum. One differentiates one-, two-, three- and four-layered silicates. The layered silicates include, for example, kaolonite, talc, clay or mica. Compositions comprising phyllosilicates and biomass terrestrial fungi, in particular the genus Ganoderma, have not been described.

Known and already used in certain products, such as Lucoton P, is the immunostimulating effect of 1.3-β-glucans (stimulation of the non-specific defense system, increase in the production of immunoglobulin A (IgA) 1.3 β-glucans are part of a polymer complex, which is obtained by alkaline digestion from the cell walls of aphyllophoraler Basidiomyceten.

The antioxidant effect of spirulina is important for use as a dietary supplement (JANA 2002). An indication that the antioxidant effect can be synergistically enhanced by combination with extracts of Ganoderma species is not found in the prior art. Known is only the combination of hot water extracts of G. lucidum with hot-water extracts of spirulina (JP 2002 24 9437 A).

II. Sunscreens based on titanium dioxide (TiO ₂ )

In conventional sunscreens, the reflection and adsorption is exploited by mineral constituents to avoid erythema (sunburn). Preparations on this basis have a high SPF and therefore allow a long stay in the sun. Inorganic UV filters are microfine particles of titanium dioxide or zinc oxide, which reflect the incident UV light. They cover the longer-wave UV-A as well as the shorter-wave UV-B range and are mainly used in sunscreens with a very high sun protection factor. Because these pigments are white, they can cause an undesirable whitening effect on the skin at higher concentrations. To prevent this, the pigment particles are reduced to about 200 nanometers - thus they are transparent.

When exposed to ultraviolet (UV) light, photocatalytic substances such as titanium dioxide (TiO ₂ ;) absorb UV radiation to a high degree. In the reaction of titanium dioxide with UV radiation, however, free radicals are formed in the presence of water and oxygen. This photocatalytic effect is used for various commercial applications such as water and air purification and gives surfaces self-cleaning, antimicrobial and anti-algae properties (DE199623055). In the photocatalytic reaction, very reactive free radicals are formed, which form a strong Chem. Res., Vol. 28, No. 12 (1995) 503 / D. Bahnemann: Photocatalytic Detoxification of Polluted Waters, The Handbook of Environmental Chemistry, Springer Verlag 1999, Volume 2, Part L, 285 - 351. An antimicrobial activity of a titanium dioxide layer on a ceramic substrate is demonstrated in DE 102 31 731 A1, which is a very strong reduction under photocatalytic activation by UV light of the starting germ content of E. iaecium to 0.01%, of S. aureus to = 0.001% and of E. coli to 0.00002%. [0018] It is therefore to be expected that by the photocatalytic effect of titanium dioxide upon application of titanium dioxide in UV protection agents in addition to premature cell aging also damage the DNA by free radicals occur whose formation is not prevented by the protective agent, but even as a result of the photocatalytic reaction is rkt.

DESCRIPTION OF THE INVENTION The object of the present invention is to find formulations with improved properties compared to the prior art which stimulate glucose metabolism, protect cells from damage and / or slow cell aging, stabilize the natural intestinal flora and bind toxins and which can therefore be used as food or feed and / or as a food or feed supplement and / or as cosmetics and / or as medicaments for therapeutic and prophylactic applications in humans and / or animals. In the case of cosmetics, in particular, a possibility should be found of combining UV protection and protection against premature skin aging by the residual UV radiation. The object has been solved according to the features of the claims. According to the invention, a composition comprising biomass and / or extracts of terrestrial fungi on the one hand and at least a) a layered silicate or an iron-rich raw material or b) fungal biopolymer complexes obtained by alkaline digestion from the cell walls of aphyllophoral basidiomycetes or c) biomasses obtainable from microalgae on the other hand , provided.

Terrestrial mushrooms have valuable ingredients that are suitable

Stimulate metabolism and protect the mammal organism from disease.

Examples of terrestrial mushrooms with as yet insufficiently used ingredients are Auricularia auricula-judae-Judasohr

Grifola frondosa - Maitake Hericium erinaceus - Hedgehog Tortoise Lentinula edodes - Shii-take Pleurotus ostreatus - Oyster Pear Pleurotus eryngii - Herbal Oyster.

Particular advantages are obtained when a species of the genus Ganoderma is used from the group of terrestrial fungi. We have surprisingly found that extracts of G. pfeifferi stimulate the glucose metabolism of cells, enhance respiration and promote the production of proteins. The administration of Ganoderma extracts also reduces oxidative stress as known in the art and enhances endogenous radical defense.

In the composition of the invention, the biomasses or the extracts of terrestrial fungi can be used.

In the embodiment of the invention in which the biomass of the terrestrial fungi are used, all ingredients are supplied in a physiologically balanced ratio to the recipient organism. It has been found that it is particularly advantageous if the biomass is present in the form of microparticles or nanoparticles with an average diameter of 10 nm-10 μm. The conversion of the biomass into microparticles or nanoparticles can be carried out according to a method known from WO 2004/075907 A2.

When mixing biomass from terrestrial mushrooms with clay minerals, a network with clay particle biomass particle contact is obtained according to the invention. By mixing biomasses and / or extracts from terrestrial fungi with biopolymer complexes obtainable from the cell walls of aphyllophoric basidiomycetes by alkaline digestion, high surface area, fibrillar porous natural product products are formed, the water-insoluble acid-stable polymer complex serving as a digestive and water binding fiber. When biomass and / or extracts of terrestrial mushrooms are mixed to form biomasses of marine organisms, obtainable from microalgae, the organism is supplemented, in addition to the ingredients of the selected terrestrial fungi, with proteins, special fatty acids, in particular cell protective omega-3 and omega-6 fatty acids, Minerals and vitamins as well as secondary ingredients supplied in a physiologically engineered ratio. Both phyllosilicates as fungale Biopolymerkomplexe, biomass from marine organisms as well as commercial mineral mixtures have insufficient radical scavenging properties. The preparation of the invention may contain biomass and / or extracts of terrestrial fungi and phyllosilicates, fungal biopolymer complexes and / or biomasses of microalgae and mineral mixtures as two, three or four combinations, and allows good protection against free radicals. In the combinations practically all mixing ratios are possible. This allows various applications that can not be achieved with the individual components. The combination according to the invention provides animal and human with high-quality nutrients, ferments, active substances, growth and protective substances in a balanced ratio to fiber and thus strengthens the organism. In an application of the composition according to the invention as a food or feed supplement, the glucans contained in the combination stimulate the immune system. The dietary and probiotic properties of the combination stabilize the natural intestinal flora. This is aided by the ability of phyllosilicates and fungal biopolymer complexes to resorb bacterial toxins. The combination simultaneously stimulates cell metabolism. It minerals and trace elements are supplied. When used as a food additive in advance or for the prevention of colds or as a feed additive in special stress situations, it is advantageous to select the species Ganoderma pfeifferi from the genus Ganoderma. In this case, the composition can be chosen so that a fight against bacterial and viral pathogens by the known contained in G. pfeifferi antimicrobial agents is possible. Free radical damage, responsible for many aging processes of cells and organs, is diminished.

Surprisingly, it has been found that even an addition of 1% of the significantly more expensive in the production of Ganoderma extracts sufficient to achieve a Radikalfänger- effect. In this way, inexpensive preparations are available which combine the above-mentioned advantages and radical scavenging property. Many foods have no experimentally detectable radical scavenger capacity. This deficiency is eliminated by the addition according to the invention of extracts of Ganoderma species as food or feed supplements.

Advantageously, 1 part of the ethanolic extract of a Ganoderma species is used to 99 parts of the food. With the combination according to the invention special effects in terms of the supply of minerals and trace substances can be achieved. In particular, it is in accordance with the invention to mix extracts of terrestrial mushrooms with biomasses obtainable from microalgae, in which a biotope is obtained during biotechnological production Enrichment with selenium and / or zinc is done. Due to special cultivation conditions, the selenium is firmly incorporated in the cell structure. The radical scavenging properties could only be detected after biodegradation or after digestion. The combination according to the invention with extracts of terrestrial fungi gives a preparation in which, with regard to the antioxidative properties, an immediate and a depot effect can be detected experimentally.

For various purposes, it is advantageous to combine the preparation according to the invention with minerals. Minerals themselves have only an insufficient radical scavenger effect. But they are often used to care for older people who need special protection against free radicals. The addition of mineral mixtures of the elements Na, K, Ca, Mg, Fe and the trace elements P, Si, B, F, Mn, Zn, Cr, Cu, V, Mo to terrestrial fungi, an optimal supply of minerals is associated with radical scavenging properties , Optimized calcium-containing minerals in combinations with antioxidant fungal ingredients of the preparation according to the invention offer, for example, a great potential in the prophylaxis of osteoporosis. Inventive combinations of Ganoderma extracts with zeolites and other ion exchangers cause a synergistic antioxidant effect. The microporous structure of the zeolites, by their ability to absorb free radicals, promotes anti-oxidant activity. Incorporated substances such as selenium are reinforced in their effect. If the addition according to the invention of alcoholic extracts from a Ganoderma species of a combination of zeolites with important minerals and trace elements is added, both the radical scavenging activity can be improved and the assurance of a balanced balance of macro- and microelements in the organism can be achieved.

Also, when used as a dietary supplement with the aim of slowing down the aging rate and delaying the onset and progression of age-associated diseases and limitations such as immune senescence, cancer, cataract, cognitive deficits, and muscle strength loss, it is particularly advantageous to use the Species of the genus Ganoderma select the species Ganoderma pfeifferi. Quite surprisingly, we found that Ganoderma pfeifferi slows the aging of cells. Under the same experimental conditions, extracts of G. lucidum do not show any slowing of the aging process. This effect of G. pfeifferi can not be deduced from the known prior art. Advantageously, an ethanolic extract of G. pfeifferi is used, wherein the ethanol content can vary within the limits of 10 vol.% To 96 vol.%. It is also possible to use mixtures of alcohols and ethyl acetate as the extraction agent. It is also possible to use extracts obtained by treating a Ganoderma species with supercritical CO ₂ .

In an advantageous embodiment of the invention, extracts of 5 different Ganoderma species and / or extracts of other terrestrial fungi are mixed. In particular, it is advantageous to mix the aqueous extract of G. lucidum with the ethanolic extract of G. pfeifferi. As we have surprisingly found, lipophilic extracts, in particular the alcoholic extract of G. pfeifferi, contain active substances which stimulate the metabolism of healthy cells and reduce cell aging. The aqueous extract of G. lucidum contains agents which activate phagocytosis by macrophages and stimulate the immune response. However, the glucan content of the aqueous extract, which stimulates the immune system, is lower in G.pfeifferi than in G. lucidum. The combination of the ethanolic extract of G. pfeifferi and the aqueous extract of G. lucidum produces the desired effect.

I ₅

It is also completely surprising that with the preparation according to the invention, natural substances for the UV protection can be made available that the beneficial reduction of UV radiation through the network with clay particle biomass particles contact with the newly discovered anti-aging - Connect effect of G. pfeifferi. This can be counteracted in particular 20 of the premature aging of the skin by the action of the UV residual radiation.

It was surprisingly found that, for example, the clay raw material "Friedländer clay" (FIM Friedland Industrial Minerals GmbH, Berlin), without further additions only

2 _{5 has} a relatively low UV adsorption, by additions of biomasses of Ganoderma pfeifferi in the amount of 1 to 10% over the entire wavelength range of 250 to 400 nm comparable to the commercial preparations based on titanium dioxide, z. T. even has superior UV absorption (or reflection). It has proven to be particularly favorable when the biomass from Ganoderma species in the form of micro- and nanoparticles with

30 have a mean diameter of 10 nm - 10 microns. The use of nanoparticles of a Ganoderma species in combination with extracts of the same or a different type has proved to be advantageous. As species of the genus Ganoderma, Ganoderma lucidum, Ganoderma pfeifferi, Ganoderma applanatum, G. resinaceum or G. adspersum can be advantageously used. Surprisingly, we have these species

3 ₅ found that effective mechanisms allow proliferation even under strong UV radiation. It is according to the invention to exploit this outstanding property of these fungi in UV protection preparations. When using the combination according to the invention, the skin cells are also protected from the effect of residual UV radiation. The direct damage to cells by UV radiation, which manifests itself, inter alia, in an LDH release, is largely avoided both by extracts of G. pfeifferi and the preparations according to the invention. Thus, the UV-related aging of the skin can be delayed. The surprisingly found anti-aging effect complements the protective effect and finds in the prevention of premature aging of the skin a very advantageous application. In contrast to UV-based titanium dioxide, which promotes the formation of free radicals under UV radiation by photocatalysis, G. pfeifferi extracts and / or biomass from terrestrial fungi reduce oxidative stress and enhance endogenous radical defense , This effect also helps to prevent premature aging of the skin.

From the group of phyllosilicates can be used for the UV protection in a particularly advantageous manner kaolins, clays, bentonites and other clay mineral-containing rocks. Non-clay mineral, but Fe-rich raw materials are used. Gaps associated with individual clay mineral groups, such as in kaolins e.g. in the range 280 nm, can be safely avoided by the inventive combination with biomasses from Ganoderma species.

The UV adsorption can be increased by selecting raw materials with Fe-rich clay minerals and other Fe-rich mineral admixtures. In addition, it is according to the invention to pretreat such raw materials and clay minerals in particular with regard to their emulsion properties, their other behavior for crosslinking with the natural organic active ingredients and the availability of Fe.

As described, for applications of the composition of the invention, a clay particle-biomass particle contact network is essential. The formation of this network can be influenced, for example, by: pH change

Change in particle size distribution and separations (by milling, sieving, sludge, sedimentation, flocculation, hydrocycloning, air classification, charge-differentiated separation, solution and precipitation reactions, delamination)

- modification of the surface morphology (activation by means of acid or soda, calcination)

- change in the chemical composition of individual minerals or the total sample (solution and precipitation reactions, ion exchange, calcination) - Change in the ion concentration in the sample (by adding ions and / or molecules, dilution, soda activation, ion exchange, change in humidity)

Any influence on the material known from the prior art, which entails a change in the emulsion properties, also leads to an influence on the network of clay minerals and biomass micro- and nanoparticles and is therefore included in the invention. This makes it possible to differentiate the network

Adjust tasks.

The process for the preparation of the preparations may be carried out in such a way that the clays dried and / or ground and / or separated and / or otherwise surface-treated, then with a water / oil emulsion, in which either the

Biomass of a Ganoderma species or extracts from a Ganoderma were incorporated in

Ratio 1: 10 to 1: 1 are mixed, and both fruiting body and the mycelium can be used for biomass and extract extraction.

I ₅

A particularly advantageous method for the preparation of the compositions according to the invention is achieved by cultivating the fungus of the genus Ganoderma on non-fermentable leaves of Camellia sinensis, which optionally wood extracts of oak and / or beech are added. It is also possible that

20 culturing the Ganodema species on mixtures of clay-containing minerals and leaves of Camellia sinensis perform the mixture after formation of the mycelium to dry, grind, separate and surface-treated then added to cosmetic preparations. In the preparation of the preparations according to one of the methods of the invention

2 ₅ nourishing or healing agents can be added.

The conversion of the biomass contained in the composition into micro- and nanoparticles, which can be done by known methods by emulsion formation and homogenization, allows the targeted doping of biomass with other active ingredients. Since both clay minerals and biomass are more terrestrial

30 mushrooms have a high absorptive power, in this way a controlled release of the active ingredient allows.

The invention therefore also health care products for humans, animals and plants for different applications based on phyllosilicates and / or

3 ₅ biomass of microalgae and / or fungal Biopolymerkomplexen and / or

Mineral mixtures, characterized by a content of 0.1 to 50%, in particular from 1 to 3% of a cold or hot alcoholic extract of a species of the genus Ganoderma, relative to the total health care product.

Finally, the invention encompasses the use of the synergistic combinations according to the invention in cosmetic preparations in the form of oils, sprays and / or ointments, alone or in combination with other cosmetics or additives, including insect repellents for use in the outdoor sector.

The features of the invention will become apparent from the claims also from the lo description, wherein the individual features each individually or to several in the form of combinations represent advantageous protective embodiments for which protection is sought with this document.

The invention will now be illustrated by some examples, without limiting the invention to these examples.

I ₅

embodiments

20 General:

The preparations according to the invention can be prepared in the following way.

. 2 ₅ a) preparation of micro- and nanoparticles from biomasses terrestrial fungi such as in the patent WO 2004/075907 A2 Az (Examples 1-4) or of extracts by generally customary methods (exemplified in Examples 32 - 33) where Extractants with different lipophilicity can be used (shown schematically in FIG. 5)

B) recovery and preparation of the phyllosilicates, the following examples

Alternatives are described (Examples 7-9) c) Mixture of clay minerals and biomasses (Example 10)

3 ₅ Example 1: Production of microparticles and nanoparticles from biomass of Ganoderma pfeifferi according to WO 2004/075907 A2 with addition of vitamin C as an example of a water-soluble active substance and of prednisolone as an example of a lipophilic active substance

Table 1: Formulation for the preparation of nanoparticles of a water-soluble active substance and a lipophilic active substance

Biomass of fruit bodies from Ganoderma pfeifferi was used. For the preparation of doped preparations, 5 g of vitamin C or 1 g of prednisolone were incorporated into the biomass.

Separately, an aqueous emulsifier solution is heated to the appropriate temperature (50 ° C). Thereafter, both phases are combined at the desired homogenization temperature. The mixture is processed with the aid of an Ultra Turrax T25 from Janke and Kunkel GmbH & Co KG (Staufen, Germany) in an emulsification process at 8000 revolutions per minute and a duration of 30 seconds. The suspension is then homogenized four times with a Micron Lab 40 piston-gap high-pressure homogenizer (APV-Gaulin, Lübeck) at a pressure of 500 bar and a temperature of 50.degree. In the production of loaded biomass, the proportion of active ingredient / clay mineral is replaced by demineralized water.

Example 2: Preparation of Micro and Nanoparticles from Mycelium of Lentinula edodes Table 2: Formulation of Lentinula edodes - (Shii-take) - Micro- and Nanoparticles

The further processing is carried out according to Example 1

Example 3: Production of micro- and nanoparticles from the fruiting body of the fungus Judasohr (Auricularia auricula-judae)

Fruit bodies of the Judasohr mushroom were used.

Recipe of the Judas ear (Auricularia auricula-judae) fungi micro- and nanoparticles as example 2

The biomass is dispersed at a temperature of 25 ° C in an aqueous emulsifier and further processed as in Examples 1 and 2.

Example 4 Production of Micro- and Nanoparticles from the Fruiting Body of the Mushroom Ganoderma lucidum - (Glossy Lackporling)

There were fruiting bodies of Ganoderma lucidum fungi used (recipe and methodology as Example 1-3).

Example 5: Examples of sheet silicates which are suitable for the compositions according to the invention.

The following sheet silicates were used:

Sample Overview

Smectite: Wyoming - Na-montmorillonite (commercial product MX-80)

County of Crook, State of Wyoming / United States Garfield - Nontronite from Garfield Area, Washington State / USA

Sound: Thierfeld - reference tone

Thierfeld - Na-dithionite treatment according to MEHRA & JACKSON (1960) Friedland-Ton (FIM GmbH, Berlin, location: Friedland, MV) Friedland HCl - treated with 2.0 M HCl at 80 ° C for 2 h

Kaolin: Wolfka - reference kaolin Table 3:

Mineralogical composition of the sheet silicate samples investigated as examples

Legend: * Wyoming: cristobalite, calcite, pyrite; ** Thierfeld: 6.4% chlorite, 4.5 hematite, 1.5% rutile / anatase; *** Friedland: calcite, dolomite, pyrite; 'mostly illite; ^m primarily montmorillonite lonit; ⁺ Friedland: kaolinite + chlorite;

Mineral form of the sheet silicates investigated as examples: GARFIELI ^GW :

Nontronite (by TEM-EDX):

"3+

Mg o.o3 Ca _0i02 Fe 0.07 Na _o .o7 K 0.02 Al _Oi2 o Fe 1.79 Mg _o .oo Ti _o .oi [(OH) ₂ Si ₃ . ₆ o Al 0.39 Fe < _o .oi Oio]

FRIEDLAND " ⁰ ^. Untreated IS interchangeable storage with an illite layer fraction of 30% (per TEM-EDX):

Mg 0.07 Ca O.O5 Na _o .o3 K ₀ .2s Al 1.19 Fe o.56 Mg _o .i9 Ti. = 0.01 [(OH) ₂ Si 3.B0 Al 0.20 Oio]

Example 6 Examination of the influence of the locus flora on the mouse ear by preparations according to the invention according to Examples 1, 5 and 6

methodology

As a model germ E. coli (strain 25922) was selected. Mouse ears were raised

Stamp clamped, disinfected with alcohol and then with a suspension of this

Model germ (germ density 7.5 x 10 ⁶ / ml) contaminated. After 1.5 h incubation at 30 ° C, the ears were rubbed with the preparation to be tested. Thereafter, they were irradiated for 30 seconds with UV light (spectrum see Figure 12). The distance skin to the lamp was

30 cm. Fig. 12 spectrum of the irradiation source

After UV irradiation, the plates were streaked onto Müller-Hinton agar. After incubation for 24 h at 37 ° C, the grown colonies were counted.

5

Results:

In the control without UV irradiation, which simulates the normal site flora, a colony count of 192 KbE was observed, which is reduced by the UV irradiation to 160 KbE. With the commercial preparation L'oreal - Solar expertise LSF 15, the local flora is severely damaged by the photocatalytic effect.

In contrast, no negative influence on the locus flora was observed in any of the tested preparations according to the invention.

The exemplary products A and B were prepared according to Example 10. In product A according to the invention, obtainable by mixing micro-i ₅ and nanoparticles from G. pfeifferi without doping according to example 1 and Wyoming montmorillonites, prepared according to example 5, 235 KbE were counted. In product B according to the invention, obtainable by mixing microparticles and nanoparticles of G. resinaceum according to example 1-3 and Wyoming-montmorillonite, prepared according to example 5, an increase to 365 KbE was even observed. The remaining audited

20 preparations according to the invention were between 235 and 365 CFU.

In the case of the phyllosilicates used as starting material, a negative influence on the locus flora was observed, for example in Garfield Nontronite at 21 KbE, Thierfeld - Reference Clay at 16 KbE and in Friedland clay at 120 KbE.

2 ₅ Example 7: Grain size refinement and mineral enrichment of phyllosilicates with permanent charge and swelling capacity

Methodology:

Bentonite Garfield <63 μm: Air-dry sample, hand-ground and sieved <63 μm, 30 - Bentonite Garfield <6.3 μm: 30 g of air-dry original sample is slurried in 2 L 0.01 N NH ₄ solution; Dispersed for 24 h in the "overhead shaker", decanted in the Attaberg cylinder at 30 cm drop height at 20 ° C ambient temperature and after 125 min, this suspension dried at 40 ° C and mortared

3 ₅

Methodology of Microbiological Investigations s. Example 6 Results

We have found that in sheet silicates having a permanent charge and swellability of the particles (e.g., smectite group minerals), grain refinement and, associated therewith, enrichment, e.g. the smectite phase, the network of phyllosilicate particles and biomass particles stabilizes better.

The influence on the UV transmission is relatively low, but the location flora is largely spared (colonies E. coli after 30 sec UV irradiation: Garfield <63 μm + Ganoderma = 151, Garfield <6.3 μm + Ganoderma = 305) , see Figure 1: Grain sizes differentiated UV measurement of bentonites with Ganoderma and commercial products

Example 8: Influence of grain size in phyllosilicates without permanent charge

methodology

Kaolin Wolfka coarse - air-dry sample, no grain refinement, kaolin Wolfka <63 microns - air-dry sample, ground by hand and sieved <63 microns, kaolin Wolfka <2 microns - 30 g air-dry original sample slurried in 2 L 0.01 N NH4 -Solution; 24 h in the "overhead shaker" dispersed, decanted in Attaberg cylinder at 30 cm drop height at 20 ° C ambient temperature after 21 h, this suspension dried at 40 ° C and mortared. Methodology of Microbiological Investigations s. Example 6

For results, see Figure 2: UV measurements (transmission) on mixtures of phyllosilicates and Ganoderma

It can be seen that the synergistic effect in the mixture of phyllosilicates with biomasses of Ganoderma species can be detected in a very large particle size range.

Advantages are obtained in the selectively coarse held Wolfka sample compared to the two finer Wolfka samples in the maintenance of the skin flora (colonies E. coli after 30 sec UV irradiation: Wolfka coarse = 313, Wolfka <63 microns = 114, Wolfka <2 μm = 130). EXAMPLE 9 Influence of the Iron Content on the Formation of the Clay Mineral Particle Biomass Particle Network Using Mixtures of Ganoderma Biomass with Bentonite or Clay

methodology

Used phyllosilicates:

Garfield - Nontronite: (air-dried, hand-mined <63 μm), 31.79 Fe ₂ O ₃ Friedland clay: (rich IS alternating deposits, air-dried, hand-mined <63 μm), 7.31 Fe ₂ O ₃

Methodology of Microbiological Investigations s. Example 6

Results

see FIG. 3: UV measurements on mixtures of Garfiel Nontroni or Friedland clay and Ganoderma

While in mixing phyllosilicate with an ointment base, the Fe content of the clay minerals has a serious effect on the UV absorption properties (left picture) was surprisingly much more significant influence of the stabilization of the network by the mineral used. Although Friedland clay has a much lower Fe content than Garfield bentonite, the better crosslinking structure of the Friedland clay mineral particles results in significantly better UV absorption results when the site flora are preserved (FIG. 3 right picture).

Example 10 Preparation of the Preparations According to the Invention from Biomass Terrestrial Mushrooms and Phyllosilicates

Recipes a) 0.1 g phyllosilicate (<63 μm triturated) + 1.0 g wool wax alcohol ¹ as control b) 0.1 g phyllosilicate (<63 μm triturated) + 1.0 g wool wax alcohol ¹ 1 ml of a 5% suspension of microparticles and nanoparticles according to Examples 1 to 4

¹ wool wax alcohol previously mixed with glycerol (1.0 g WW + 0.2 g glyc)

The preparation is obtainable by simple mixing and by trituration in a mortar. Example 11 Release of Prednisolone from the Products According to the Invention According to Example 1

methodology

The release was determined by means of paddle apparatus. The particle dispersion to be tested is placed directly in the round-bottomed vessel and samples are taken at certain times. The particles contained in the measuring solution are filtered off and discarded. You will be deprived of the further release process. To compensate, the sample volume is not replaced, so it can be assumed that the output ratio of particles to dispersion medium remains constant during the course of the experiment. The filtrate is analyzed by UV spectrometry for its active ingredient content. Membrane filters made of polyamide from Schleicher & Schuell (Dassel, Germany) with a pore size of 0.2 μm were used for the filtration.

The measurement conditions for determining the release of active ingredient were defined as follows:

Acceptor fluid: 1000.0 ml of demineralized water

• Test temperature: 25 ° C • Stirring frequency: 100 min ^"1

• Sample volume: 3 ml

Results

The drug prednisolone was released continuously and in a controlled manner within 4 days.

see Figure 4: Time course of drug release from Ganoderma particles that were doped with 1% prednisolone (based on the actual content)

Example 12: Detection of the Protective Effect of Ganoderma Extracts on Skin Cells

methodology

Under standard conditions (37 ° C, 95% humidity, 5% CO ₂ , RPMI medium with 8% FCS) adherent human cells (HaCaT, DKFZ) were cultured in 60mm ² cell culture dishes to a confluency of 50%. The medium was replaced by 2 ml PBS and the Cells irradiated with a dose of 20mJ / cm ² UV light (medical UVB, broadband, maximum 311nm). The preparation was carried out under standard conditions as shown in the scheme (Figure 5 extraction scheme for the production of mushroom extracts).

5 results

Ganoderma extracts can significantly reduce UV-induced damage to the keratinocytes.

see FIG. 6: LDH release from HaCaT cells of Ganoderma extracts after irradiation with medical UVB

Example 13: Demonstration of the protective effect of the preparations according to the invention on skin cells

I ₅

Methodology s. Example 12

In comparison to unprotected cells, (•) reduction of the phototoxic effects became visible after 10 hours after irradiation (A, ■). The strong damage, which becomes visible at the control from the 16 h, are surely prevented.

20 see FIG. 7: LDH release from HaCaT cells after irradiation with medical UVB (broadband)

2 ₅ Example 14: Evidence of the anti-aging effect

Methodology:

An experimental setup was used, which in. DP 19709649.2 (Jülich, W. -D., Woedtke, Th. Of, Abel, P .: Measuring arrangement for the detection of toxic, subtoxic, chronic toxic or stimulant effects of active substances and pollutants with the aid of

30 perfusion cell cultures). Confluent FL cells were treated with mitomycin C to inhibit growth (described in detail in Example 15). The cell cultures were fed from the 20th to the 30th h with a commercial medium (Dulbecco's Minimal Essential Medium with 10% calf serum), to which was added 0.025 mg of an ethanolic extract of G. pfeifferi per ml culture medium.

3 ₅ Thereafter, the Perfusionszellkultur was continued with medium without additives. It became the Time measured, in which the glucose metabolism reduced to 50% of the initial value (before the administration of the enriched with G. pfeifferi medium).

Results see Figure 8: Influence of Ganoderma pfeifferi in the 21.-31.Std on the extension of the 5 cell life in a perfusion cell culture measured on glucose consumption (mmol / 1).

G. lucidum (not shown) has an opposite effect. The cells die here already after 96h.

As a result of cell aging, the glucose metabolism decreases after about 150 hours. lo A single treatment with G. pfeifferi extract delays this development (Table 4).

Tab.4 Anti-aging effect of G. pfeifferi extract

i ₅ The result is significant (t-test in pairwise comparison) with an error probability <1%.

Example 15: Detection of the stimulating effect by the preparations according to the invention on stationary cell cultures by determining the protein content of the cells

methodology

Confluent FL cells are treated with mitomycin C to inhibit growth as follows: pour medium from tissue culture flask, wash cell lawn 2 times ₅ times with PBS, 5 ml commercial medium (DulbeccoMEM + antibiotics + 10% fetal calf serum + 1% L glutamine) Add 50μl mitomycin C (50μg) to the cells, incubate for 2h at 37 ° C and 5% CÜ ₂ in the incubator, drain the mixture and wash 3 times with PBS. The cells were then trypsinized, in a cell density of 2x10 ⁵ / ml medium in

Saw microtiter plates and cultured at 37 ° C and 5% CO ₂ .

After 24 hours, the medium was removed, Ganoderma extract in medium 5 (25 .mu.g / ml) added to the cells and then further cultured for 24 h at 37 ° C and 5% CO ₂ . The control cultures received fresh culture medium without extract.

After washing 5 times with PBS, the cells were mixed with 100 μl of a 0.04%

Triton solution per well and shake for 30 min on a shaker (500rpm) lysed.

The protein determination was carried out with Roti-Nanoquant and a B SA standard as lo calibration solution.

The extinction was measured at 450 and 620 nm and for the evaluation of the quotient

OD620 / 450nm formed.

The protein concentration was determined on the basis of the quotients from the B SA standard curve.

I ₅

Results

The administration of ethanolic G. pfeifferi extract can stimulate cell metabolism. The protein content is increased on average by about 15% (Table 5). This effect is highly significant.

20

Table 5 Protein contents (μg / ml) of cells after cultivation with and without G. pfeifferi extract

2 ₅ Example 16: Detection of the Stimulating Effect - Comparison with Ginseng

This effect is compared with a known product (ginseng), which is widely used as an anti-aging agent. Again, the effects in stationary cell culture are examined.

Methodology See example 15

Results

The G. pfeifferi hot extract used caused a very marked stimulation of the cell metabolism. The protein content of the cell increased by 20%. In the study of ginseng extracts 25 μg / ml have practically no influence, only doubling the concentration to 50 μg / ml causes an increase in protein production by 5%. In the examination of the finished preparation even a lower protein content was observed.

FIG. 9: Protein content (μg / ml) of cells after cultivation with and without G. pfeifferi in comparison with G. lucidum and ginseng

Example 17: Protection of the cells from harmful influences

methodology

Under standard conditions (37 ° C, 95% humidity, 5% CO ₂ , RPMI medium with

8% FCS) adherent human cells (HaCaT, DKFZ) were grown in 50 mm ² cell culture dishes to a confluence of 50%.

In order to simulate a stress situation, the medium was replaced by 2 ml PBS and the cells irradiated with a dose of 20mJ / cm ² UV light (medical UVB, broadband, maximum 311nm). In PBS, extracts of Ganoderma species were dissolved at a concentration of 10 μg / ml (Extr. 1) or 0.01 μg / ml (Extr. 2). Subsequently, the PBS and the extract dissolved therein was removed and replaced with RPMI medium with 0.01% BSA (bovine serum albumin). In the following 10 hours, the morphological development of the cells and the release of the cytoplasmic enzyme lactate dehydrogenase were followed. Control cells were also treated, but without being actually irradiated.

FIG. 10: LDH release from HaCaT cells after irradiation with medical UVB (broadband) The extracts used were able to reduce the damage caused to the cells by UV radiation (FIG. 9). In particular, the Ganoderma-Extr. 1 (A) gives up to a 5-fold protective effect. For the less concentrated Ganoderma 5 Extr. 2 (■) an approximately 1.5 fold protective effect for the cells could be detected.

Titanium dioxide, which is very commonly used in sunscreens, enhanced by the photocatalytic effect, the damaging effect of UV rays. Titanium dioxide was used in direct comparison as a suspension of titanium dioxide nanoparticles in PBS with a dose of 3 μg / cm ² . (Fig. 10)

Example 18: Detection of the protective effect of the preparations according to the invention on skin cells in comparison to TiO ₂

I ₅

Methodology: s. Example 17

The experimental setup was modified in that in the beam path either only a quartz plate (control) or a quartz plate with a corresponding preparation of the invention (clay mineral plus biomass, see Example.10 production) or 20 TiO ₂ (in the form a nanoparticle suspension). The quartz plate alone does not absorb any UV light in this area. Subsequently, the PBS was removed and replaced with RPMI medium containing 8% FCS. In the following 12 hours, the morphological development of the cells and the release of the cytoplasmic enzyme lactate dehydrogenase were followed.

2 ₅

Results

FIG. 11: LDH release from HaCaT cells after irradiation with medical UVB (broadband), preparation from G. pfeifferi and Friedländer clay in comparison to TiO ₂

30

Lactate release is increased under the influence of TiO ₂ over the control

Figure. 7: LDH release from HaCaT cells after irradiation with medical UVB (broadband). Preparation of G.pfeiferi and bentonite Garfield <6.3 μm)

3 ₅

TiO ₂ has an unfavorable effect on skin cells even in this experimental setup in which the photocatalytic effect is not detected. In contrast, the Lactate release as a measure of UV-induced damage under the influence of the preparation according to the invention (Example 1 in Fig. 11: Preparation obtainable from G.pfeifferi and Friedlander Ton; Example 2 in Fig. 7 (H): Preparation from G.pfeiferi and bentonite Garfield <6.3 μm) lower than the controls.

This experiment was repeated with further preparations according to the invention, wherein the observation time was increased to 36.

Example 19: UV protection

methodology

The UV transmission was measured with the UV photometer Analytik Jena AG SPECORD 50.

Lamp: 483 V, 0.3 A, 200 Hz; Distance sample lamp: 10 cm; Preparation each 0.003 mm thick; Quartz cuvette; Measured value = average value of 20 repeat measurements (each new preparation feed)

Results

With the preparations according to the invention a strong, comparable with commercial preparations with the sun protection factor 15 reduction of UV transmission is achieved. In contrast, the reduction in UV transmission through the clays used as starting material is significantly lower.

see FIG. 13 (right) UV measurements on mixtures of phyllosilicates and Ganoderma

Example 20: Investigation of the compositions according to the invention based on biomass of microalgae of the genus Spirulina enriched with selenium in biologically bound form (selenium spirulina) with and without digestion by heat and pressure

The determination of the antioxidative properties was carried out by thin-layer chromatography (SIEVERS A et al., (2002) Simple thin-layer chromatography test for antioxidative compounds using DPPH assay, CBS Camag Bibliography Service 88: 14-15) and photometrically (MOLYNEUX P (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity: Songklanakarin J Sei Technol 26 (2): 211-219). Selenium spirulana with a selenium content of 7.5 mg selenium / 100 g has no detectable radical scavenging properties with these test systems. The thin-layer chromatographic detection is very sensitive. Concentrations> 0.005% of an antioxidant substance are detectable. By adding 1% G. lucidum extract, an immediately detectable radical scavenging activity of 94% is achieved. An addition of 10% causes a radical scavenging activity of 95.1%.

By contrast, after a digestion by treatment in an autoclave, which is to simulate the biological utilization of biomass in the organism, a radical scavenging activity of 71% can be detected. The selenium-spirulina bound selenium can therefore sustainably stimulate the synthesis of important peroxidase enzymes (e.g., glutathione peroxidase) which the body desperately needs to fight off the free radicals.

I ₅

Example 21: Examination of Combinations Based on the Biomass of

Microalgae of the genus Spirulina enriched with zinc in biologically bound form (zinc spirulina)

20 Methodology: s. Example 20

Zinc spirulina has a radical scavenging capacity of 20.3%. By addition of 1% ethanolic extracts of G. lucidum or G. pfeifferi immediately detectable radical scavenging activities of 74, 1, by adding 10% of the extracts 92.3% are achieved.

2 ₅ Example 22: Investigation of the biomass of microalgae of the genus Chlamydomonas

Methodology s. Example 20

[Olli] Biomass, obtainable by cultivating microalgae of the genus Chlamydomonas, has a radical trapping property of 14.8%. By adding 1 30% ethanolic extract of G. lucidum or G. pfeifferi, immediately detectable radical scavenging activities of 77, 3 are achieved by adding 10% of the extracts> 90%.

Example 23: Investigation of the biomass of microalgae of the genus Porphyridium

3 ₅

Methodology s. Example 20 Biomass, obtainable by culturing microalgae of the genus Porphyridium, have only a low radical scavenging property of 4%. By adding 1% ethanolic extracts of G. lucidum or G. pfeifferi, immediately detectable radical scavenging activities of 40, 7 are achieved by adding 10% of 94%.

EXAMPLE 24 Examination of the Agents of the Invention Based on the Fungal Biopolymer Complex Lucoton T

The complex was recovered by alkaline digestion from the cell wall according to Example 29.

The preparation has the following advantageous application properties: Bioadsorption of heavy metals and toxins, but no adsorption more vital

Macro- and microelements, - strengthening the immune system by stimulating the nonspecific defense system and stimulating the anti-inflammatory cytokines

The complex has no doping with Ganoderma extract on the detectable with the methodology of Example 20 radical scavenging properties (radical scavenging activity 5.3%). This disadvantage is remedied by adding 1% or 10% of an ethanolic extract from G. lucidum. The radical scavenging activity thereby increases to 50 or 93%.

Example 25: Fungal biopolymer complex obtainable from oyster mushroom substrates (Pleurotus ostreatus)

The biopolymer complex was recovered by alkaline digestion from the cell walls in analogy to Example 29. The investigation of antioxidative properties was carried out according to the method of Example 20.

The biopolymer complex has a radical scavenging capacity of 5.7%. By adding 1% ethanolic extract of G. lucidum, immediately detectable radical scavenging activities of 42.2 are achieved, with the addition of 10% of the extracts 89.6%.

Also by addition of 1% of an ethanolic extract of G. pfeifferi the product receives high radical scavenging properties. Example 26: Investigation of the compositions of the invention based on mineral mixtures

Mineral mixtures using carbonates, phyllosilicates, skeletal silicates, zeolites and oxides from Durtec GmbH Neubrandenburg are used for the following indications:

• gastrointestinal diseases

• Mineral deficiency, especially Mg, Ca, and trace elements

• Skin damage, acne

10 • Treatment of the rheumatic type

For these indications, a radical scavenging activity is advantageous, which is achieved by addition of 1 to 10% of an ethanolic extract of G. lucidum (Table 6).

Results obtained according to the methodology of Example 20

I ₅

Tab. 6 Radical scavenging capacity of various mineral mixtures

Example 27: Improvement of radical scavenging properties of fish feed

[0125] The improvement of free-radical scavenging properties is also important in the case of dried fodder for fish feed.

For dried foods based on Artemia, which has a radical scavenger capacity of 15.7% without additives, increases by the inventive additions of 1 and 10% of a G. lucidum extract, the Radikalfängerkapazität to 64.6 and 90.9 %. Example 28: Improvement of the radical capacity of a commercial product with high radical scavenging capacity

Also in preparations that have been developed specifically for anti-aging purposes

5 ("beauty from within") and therefore a complex composition with already high

Have radical capacity, the antioxidant properties can be improved. In the commercial preparation Inneov (Laboratoires Inneov, France), a

Dietary supplement to support the tissue density of mature skin with the

Ingredients sugar, cellulose, calcium phosphate, manit, talc, lacto-lycopene, lo soy extract and vitamin C, the radical scavenger capacity increased by the addition of 1 and 10 respectively

% of a Ganoderma extract from 91.4 to 96.4 and 98.9%, respectively.

Example 29: Preparation of Fungal Biopolymer Complexes from Tinder Sponge (Fomes fomentarius)

I ₅ a) Coarse ground sponge (Fomes fomentarius) coarsely crushed with KOH is added to a three-necked flask fitted with stirrer, thermometer and reflux condenser with 900 ml of dist. Added water and heated to 60 ° C with stirring. Thereafter, 8 g of potassium hydroxide

20 (Applichem, purest) in 100 ml dist. Dissolved water and heated the mixture for three hours at 90 to 95 ° C.

After the reaction is allowed to cool and sucks the reaction mixture through a suction filter. The filter residue is distilled five times with 1 l each. Washed water and then dried in a drying oven at 80 ° C. Yield: 54 g, corresponding to 67.5% of the used

2 ₅ mushroom amount.

b) with NaOH

80g crushed cinder sponge (Fomes fomentarius) are in a three-necked flask with stirrer, thermometer and reflux condenser with 900 ml dest. Water 30 is added and heated to 60 ° C with stirring. Thereafter, 6 g of sodium hydroxide (Merck, for analysis) in 100 ml of dist. Dissolved water and heated the mixture for three hours at 90 to 95 ° C.

After the reaction is allowed to cool and sucks the reaction mixture through a suction filter. The filter residue is distilled five times with 1 l each. Washed in water and then in Drying oven dried at 80 ° C. Yield: 52.8 g, corresponding to 66% of the amount of fungus used.

Example 30: Preparation of fungal biopolymer complex from oyster mushroom

a) with KOH

50g dried and ground oyster mushroom (Pilzhof Krummensee) are in one

Three-necked flask with stirrer, thermometer and reflux condenser with 500 ml of dist. Added water and heated to 60 ° C with stirring. Thereafter, 5 g of potassium hydroxide

(Applichem, purest) in 50 ml dist. Dissolved water and heated the mixture for three hours

90 to 95 ° C.

After the reaction is allowed to cool and sucks the reaction mixture over a

Suck it off. The filter residue is distilled five times with 1 l each. Washed water and then dried in a drying oven at 80 ° C. Yield: 16 g, corresponding to 32% of the used

Mushroom amount.

b) with NaOH

50g dried and ground oyster mushroom (Pilzhof Krummensee) are in a three-necked flask with stirrer, thermometer and reflux condenser with 500 ml dest. Added water and heated to 60 ° C with stirring. Then add 3.5 g of sodium hydroxide (Merck, for analysis) in 50 ml of dist. Dissolved water and heated the mixture for three hours to 90 to

95 ° C.

After the reaction is allowed to cool and sucks the reaction mixture through a suction filter. The filter residue is distilled five times with 1 l each. Washed in water and then in

Drying oven dried at 80 ° C. Yield: 18.5 g, corresponding to 37% of the used

Mushroom amount.

Example 31: Preparation of mushroom extracts

Starting material:

• Oyster mushroom dried mushrooms • Herb oyster dried mushrooms

• Shi-take - dried, (Lentinula edodes) • Ling Zhi (Ganoderma lucidum)

Manufacturer: Pilzhof R. Schulz, 16356 Krummensee (Barnim)

a) oyster mushroom

2 g of dried oyster mushroom are ground and distilled at room temperature with 20 ml. Water is added. After 24 hours of cold extraction at room temperature, the fungi are squeezed through a filter and the extract is filtered through a round filter. 14 ml of pale yellow-colored extract are obtained.

b) Ganoderma watery

2 g of dried Ling Zhi mushrooms (Ganoderma lucidum) are ground and distilled at room temperature with 20 ml. Water is added. After 24 hours of cold extraction at room temperature, the fungi are squeezed through a filter and the extract is filtered through a round filter.

This gives 12 ml of pale yellow-colored extract.

c) Ganoderma methanolic

2 g of dried Ling Zhi mushrooms (Ganoderma lucidum) are ground and treated at room temperature with 20 ml of methanol (Merck, purest). After 24 hours of cold extraction at room temperature, the fungi are squeezed through a filter and the extract is filtered through a round filter. 14 ml of pale yellow-colored extract are obtained.

d) Ganoderma ethanolic

25 g of dried Ling Zhi mushrooms (Ganoderma lucidum) are ground and treated with 250 ml of ethanol (palatability, denatured, for analysis) at room temperature. After 24 hours of cold extraction at room temperature, the fungi are squeezed through a filter. The resulting cloudy extract is first centrifuged and then filtered through a round filter. 160 ml of yellow-colored extract are obtained. e) Extracts of Ganoderma pfeifferi

Ganoderma pfeifferi fruiting bodies were obtained by wild collection in Schlosspark Ludwigsburg in the years 1997-2005. In addition, cultured fruiting bodies, which were obtained by the company GAMU GmbH, Krefeld in April 2004, were used. 2 g of dried mushrooms (Ganoderma pfeifferi) are ground and treated at room temperature with 20 ml of ethanol (Applichem, denatured, for analysis). After 24 hours of cold extraction at room temperature, the fungi are squeezed through a filter and the extract is filtered through a round filter. This gives 12 ml of yellow-colored extract.

f) hot extracts from Ganoderma

The pulverized fruit body was mixed in a paper tube with ethanol 80% (v / v) and then extracted by the Soxhlet method for about 24 h. The weight was 12.5 g for both Ganoderma lucidum and G. pfeifferi. The recovered liquid extracts were concentrated and freeze-dried.

Example 31: Preparation of the extracts by means of an extractor

Extraction was carried out using extractor ASE 200 (Dionex, Sunnyvale, CA, USA). In each case, 2 g of powdered mushroom fruit bodies with 15 g of sea sand were weighed into a paper tube. Extraction was carried out at a pressure of 2000 psi (pounds per square inch) with a service life (residence time of the solvent in the extraction cell at each extraction step) of 2 minutes. The extractant used was EtOH 80% (v / v) at an extraction temperature of 60 ° C.

Claims

claims

1. Composition comprising biomass of terrestrial fungi and / or extracts of terrestrial fungi on the one hand and at least

5 a) a phyllosilicate or an iron-rich raw material and / or b) fungal biopolymer complexes obtained by alkaline digestion from the cell walls of aphyllophoral basidiomycetes and / or c) biomasses obtainable from microalgae on the other hand.

10

2. Composition according to claim 1, characterized in that ethanolic, aqueous or a combination of aqueous and ethanolic extracts are used.

3. Composition according to one of claims 1 or 2, characterized in that from i _{5 of} the group of terrestrial fungi biomasses or extracts of at least one kind of

Genus Ganoderma be selected.

Composition according to Claim 3, characterized in that they are species of the genus Ganoderma, preferably G. lucidum, G. pfeifferi, G. resinaceum, G. adspersum

20 or G. aplanatum.

5. Composition according to one of claims 1 to 4, characterized in that the biomass in the form of micro or nanoparticles with an average diameter of 10 nm - 10 microns are present.

2 ₅

6. The composition according to claim 5, characterized in that the conversion of the biomass into micro- or nanoparticles with an average diameter of 10 nm - 10 microns according to a known from Az. WO 2004/075907 A2 method.

30 7. Composition according to one of claims 1 to 6, characterized in that the biomass of the terrestrial fungi are doped with water-soluble or lipophilic active ingredients.

8. The composition according to any one of claims 1 to 7, characterized in that the proportion of the extract and / or biomass from terrestrial fungi from 0.1 to 20%, based on the total weight.

9. Composition according to claim 1, characterized in that from the components a) to c) phyllosilicates and fungal biopolymer complexes are selected and combined as a premix, which may additionally contain minerals, with biomasses and / or extracts of terrestrial fungi.

lo

10. The composition according to claim 1, characterized in that from the components a) to c) phyllosilicates and biomass, obtainable from microalgae, are selected and combined as a premix, which may additionally contain minerals, with biomasses and / or extracts of terrestrial fungi.

i ₅

11. The composition according to claim 1, characterized in that from the components a) to c) phyllosilicates and fungal biopolymer complexes and biomass, obtainable from microalgae, are selected and as a premix, which may additionally contain minerals, with biomasses and / or extracts of terrestrial fungi be combined.

20

12. The composition according to any one of claims 1 to 11, characterized in that it contains further minerals.

13. A composition according to any one of claims 1 to 12, characterized in that it contains 2 ₅ other substances such as vitamins or active ingredients (anti-inflammatory, wound healing, immune stimulating).

14. Controlled release compositions of care or curative agents containing at least one composition according to any one of claims 1 to 13.

30

Anti-aging and anti-aging agent containing at least one composition according to any one of claims 1 to 13.

16. Dietary supplements containing at least one composition of claims 1 to 3 ₅ 13.

17. Health-enhancing agents which improve the protection of the mammalian organism and of plants against free radicals, characterized in that they contain at least one composition of claims 1 to 13.

5 18. An agent for the prophylaxis of osteoporosis containing at least one composition of claims 1 to 13.

19. UV protection preparations containing at least one composition according to any one of claims 1 to 13.

10

20. Plant strengthening agents containing at least one composition of claims 1 to 13

21. A process for the preparation of the compositions having at least one layered silicate i ₅ or an iron-rich raw material according to claim 1, characterized by the following

Steps: a) Drying, grinding, and separating the phyllosilicates or the iron-rich raw materials. B) Producing the biomasses and / or extracts of at least one species of the genus Ganoderma

20 c) mixing the components a) and b) in a ratio of 1:10 to 1: 1.

22. A process for the preparation of the compositions comprising at least one layered silicate or an iron-rich raw material according to claim 1, characterized by the following steps:

2 ₅ a) drying, milling, and separating the layered or iron rich raw materials, b) enrichment or depletion of a mineral phase of the raw materials described under a) c) Change in the inner or outer surface of the minerals d) producing the biomass and / or Extracts of at least one species of the genus 30 Ganoderma e) Mixing of components c) and d) in a ratio of between 1:10 to 1: 1.

23. A process for the preparation of the compositions comprising at least one layered silicate or an iron-rich raw material according to claim 1, characterized by the following

3 ₅ steps: a) Drying, grinding, and separating the phyllosilicates or the iron-rich raw materials b) conversion of the biomass of at least one species of the genus Ganoderma into micro- and nanoparticles with an average diameter of 10 nm-10 μm. c) mixing the components a) and b) in a ratio of between 1:10 to 1: 1. d) incorporating the mixture into an ointment base

5

24. A process for the preparation of the compositions according to any one of claims 1 to 13, characterized in that a cultivation of a fungus of the genus Ganoderma takes place on non-fermentable leaves of Camellia sinensis, which optionally wood extracts of oak and / or beech are added.

10

25. A process for the preparation of the compositions according to any one of claims 1 to 13, characterized in that the cultivation of Ganodema species is carried out on mixtures of clay-containing minerals and leaves of Camellia sinensis, the mixture after formation of the mycelium dried, ground, separated, and / or surface-treated i ₅ and then added to cosmetic preparations.

26. Use of extracts and / or biomass from fungi of the species Ganoderma pfeifferi for the production of anti-aging agents.

20 27. Use extracts and / or biomass from fungi of the species Ganoderma pfeifferi for the preparation of dietary supplement with anti-aging effect.

28. Use of extracts and / or biomass of fungi of the species Ganoderma pfeifferi for the preparation of feed additives with antimicrobial action and anti-aging

2 ₅ effect.

29. Use of the compositions according to any one of claims 1 to 13 for the preparation of cosmetic preparations with anti-aging effect.

30 30. Use of the compositions according to any one of claims 1 to 13 for the production of roughage with anti-aging effect.

31. Use of the compositions according to any one of claims 1 to 13 for the preparation of agents for supporting tumor therapies with anti-aging effect.

3 ₅

32. Use of the compositions according to any one of claims 1 to 13 for the preparation of agents for the prophylaxis and treatment of colds with anti-aging effect.

5 33. Use of the compositions according to any one of claims 1 to 13 for the preparation of medicaments with anti-aging effect.

34. Use of the compositions according to any one of claims 1 to 13 as a sunscreen.

10

35. Use of compositions according to any one of claims 1 to 13 in cosmetic or medicinal preparations in the form of oils, sprays and / or ointments.

i ₅ 36. Use of compositions according to any one of claims 1 to 13 in combination with other cosmetics or additives.

37. Use of the compositions according to any one of claims 1 to 13 in a cosmetic preparation.

20

38. Use according to any one of claims 34 to 37 in agents in an insect repellent.