EP1815246A4 - Entwicklung von empfindlichen fret-sensoren und verwendungsverfahren dafür - Google Patents

Entwicklung von empfindlichen fret-sensoren und verwendungsverfahren dafür

Info

Publication number
EP1815246A4
EP1815246A4 EP05812048A EP05812048A EP1815246A4 EP 1815246 A4 EP1815246 A4 EP 1815246A4 EP 05812048 A EP05812048 A EP 05812048A EP 05812048 A EP05812048 A EP 05812048A EP 1815246 A4 EP1815246 A4 EP 1815246A4
Authority
EP
European Patent Office
Prior art keywords
development
methods
same
fret sensors
sensitive fret
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP05812048A
Other languages
English (en)
French (fr)
Other versions
EP1815246A2 (de
Inventor
Wolf B Frommer
Sakiko Okumoto
Loren Looger
Marcus Fehr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carnegie Institution of Washington
Original Assignee
Carnegie Institution of Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carnegie Institution of Washington filed Critical Carnegie Institution of Washington
Publication of EP1815246A2 publication Critical patent/EP1815246A2/de
Publication of EP1815246A4 publication Critical patent/EP1815246A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2814—Dementia; Cognitive disorders
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2835—Movement disorders, e.g. Parkinson, Huntington, Tourette
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2857—Seizure disorders; Epilepsy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2871—Cerebrovascular disorders, e.g. stroke, cerebral infarct, cerebral haemorrhage, transient ischemic event

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP05812048A 2004-10-14 2005-10-14 Entwicklung von empfindlichen fret-sensoren und verwendungsverfahren dafür Withdrawn EP1815246A4 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US61817904P 2004-10-14 2004-10-14
US64357605P 2005-01-14 2005-01-14
US65444705P 2005-02-22 2005-02-22
US65814105P 2005-03-04 2005-03-04
PCT/US2005/036957 WO2006044612A2 (en) 2004-10-14 2005-10-14 Development of sensitive fret sensors and methods of using the same

Publications (2)

Publication Number Publication Date
EP1815246A2 EP1815246A2 (de) 2007-08-08
EP1815246A4 true EP1815246A4 (de) 2009-12-16

Family

ID=36203532

Family Applications (1)

Application Number Title Priority Date Filing Date
EP05812048A Withdrawn EP1815246A4 (de) 2004-10-14 2005-10-14 Entwicklung von empfindlichen fret-sensoren und verwendungsverfahren dafür

Country Status (7)

Country Link
US (3) US8530633B2 (de)
EP (1) EP1815246A4 (de)
JP (1) JP5323356B2 (de)
AU (1) AU2005295656B2 (de)
CA (1) CA2584108A1 (de)
IL (1) IL183146A0 (de)
WO (1) WO2006044612A2 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE489397T1 (de) * 2005-10-14 2010-12-15 Carnegie Inst Of Washington Saccharose-biosensoren und verwendungsverfahren dafür
CA2625169A1 (en) * 2005-10-14 2007-04-26 Carnegie Institution Of Washington Methods of reducing repeat-induced silencing of transgene expression and improved fluorescent biosensors
US20100202966A1 (en) * 2006-03-28 2010-08-12 Yossi Gross Implantable Sensor
US20120122115A1 (en) * 2006-04-25 2012-05-17 Sayre Richard T Bacterial quorum sensing biosensor
JP2008154520A (ja) * 2006-12-25 2008-07-10 Meiji Univ 蛍光タンパク質を発現するトランスジェニック非ヒト動物
WO2008155304A1 (de) * 2007-06-20 2008-12-24 Basf Se Synthetische repetitive proteine, deren herstellung und verwendung
WO2009023153A1 (en) * 2007-08-10 2009-02-19 Carnegie Institution Of Washington Methods of using ret nanosensors
US20100160749A1 (en) * 2008-12-24 2010-06-24 Glusense Ltd. Implantable optical glucose sensing
US8741591B2 (en) 2009-10-09 2014-06-03 The Research Foundation For The State University Of New York pH-insensitive glucose indicator protein
CA2804205C (en) * 2010-06-30 2018-01-09 Carnegie, Institution Of Washington Method to measure the metabolic rate or rate of glucose consumption of cells or tissues with high spatiotemporal resolution using a glucose nanosensor
US9037205B2 (en) 2011-06-30 2015-05-19 Glusense, Ltd Implantable optical glucose sensing
US8652531B2 (en) * 2011-07-29 2014-02-18 Kimberly-Clark Worldwide, Inc. Indicator for oxygen generation
US10684282B2 (en) 2011-10-07 2020-06-16 Howard Hughes Medical Institute Genetically encoded biosensors
US9719992B2 (en) 2011-10-07 2017-08-01 Howard Hughes Medical Institute Genetically encoded biosensors
EP2615111A1 (de) * 2012-01-10 2013-07-17 Laboratorios del Dr. Esteve S.A. Verfahren zur Identifizierung von Liganden für Sigma-1-Rezeptoren
US10466233B2 (en) * 2012-04-13 2019-11-05 Centro De Estudios Cientificos De Valdivia Genetically encoded probe for quantification of lactate and methods for quantifying metabolic rates and lactate transport
WO2014026162A1 (en) 2012-08-10 2014-02-13 Opx Biotechnologies, Inc. Microorganisms and methods for the production of fatty acids and fatty acid derived products
US10047383B2 (en) 2013-03-15 2018-08-14 Cargill, Incorporated Bioproduction of chemicals
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP3022310B1 (de) 2013-07-19 2019-10-16 Cargill, Incorporated Mikroorganismen und verfahren zur herstellung von fettsäuren und aus fettsäuren gewonnenen produkten
WO2015025192A1 (en) * 2013-08-20 2015-02-26 Centro De Estudios Cientificos De Valdivia Genetically encoded probe for quantification of pyruvate concentration and methods of using the same
EP2993228B1 (de) 2014-09-02 2019-10-09 Cargill, Incorporated Herstellung von fettsäureestern
US10575765B2 (en) 2014-10-13 2020-03-03 Glusense Ltd. Analyte-sensing device
DE102015115640B4 (de) * 2015-09-16 2017-06-29 Stiftung Caesar Center Of Advanced European Studies And Research Verfahren zum Nachweis von Liganden vermittels Biosensoren
WO2017087051A1 (en) * 2015-11-20 2017-05-26 Duke University Glucose biosensors and uses thereof
US10871487B2 (en) 2016-04-20 2020-12-22 Glusense Ltd. FRET-based glucose-detection molecules
CN110494566A (zh) 2017-02-02 2019-11-22 嘉吉公司 产生c6-c10脂肪酸衍生物的经遗传修饰的细胞
KR102660777B1 (ko) * 2017-08-08 2024-04-24 커먼웰쓰 사이언티픽 앤드 인더스트리얼 리서치 오가니제이션 탄수화물 센서
WO2021033015A1 (en) 2019-08-22 2021-02-25 Centro De Estudios Científicos De Valdivia Genetically encoded probe for detection and quantification of lactate/pyruvate ratio with high spatial and temporal resolution

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006044611A2 (en) * 2004-10-14 2006-04-27 Carnegie Institution Of Washington Neurotransmitter sensors and methods of using the same
WO2006096214A1 (en) * 2005-03-04 2006-09-14 Carnegie Institution Of Washington Environmentally stable sensors and methods of using the same
WO2006096213A1 (en) * 2005-03-03 2006-09-14 Carnegie Institution Of Washington Polyamine sensors and methods of using the same
WO2007046786A2 (en) * 2005-10-14 2007-04-26 Carnegie Institution Of Washington Sucrose biosensors and methods of using the same

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6803188B1 (en) 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5795729A (en) 1996-02-05 1998-08-18 Biometric Imaging, Inc. Reductive, energy-transfer fluorogenic probes
US5998204A (en) 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US6376257B1 (en) 1997-04-24 2002-04-23 University Of Rochester Detection by fret changes of ligand binding by GFP fusion proteins
WO1999034212A1 (en) 1997-12-31 1999-07-08 Duke University Biosensor
EP1098658B1 (de) 1998-07-17 2009-07-08 University of Maryland, Baltimore Manipulierte proteine zum analyten nachweis
WO2000049183A1 (en) 1999-02-17 2000-08-24 Clontech Laboratories, Inc. FLUORESCENCE RESONANCE ENERGY TRANSFER DETECTION OF cAMP IN LIVING CELLS USING GFP VARIANTS
US6465199B1 (en) 1999-02-26 2002-10-15 Cyclacel, Ltd. Compositions and methods for monitoring the modification of natural binding partners
US6469154B1 (en) 1999-05-21 2002-10-22 The Regents Of The University Of California Fluorescent protein indicators
AU7367300A (en) 1999-09-10 2001-04-10 University Of Maryland At Baltimore Inactive enzymes as non-consuming sensors
CA2314398A1 (en) 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
US7687614B2 (en) 2001-02-26 2010-03-30 The Regents Of The University Of California Monomeric and dimeric fluorescent protein variants and methods for making same
WO2003025220A2 (en) 2001-09-18 2003-03-27 Carnegie Institution Of Washington Fusion proteins useful for detecting analytes
US20040029129A1 (en) * 2001-10-25 2004-02-12 Liangsu Wang Identification of essential genes in microorganisms
US6855556B2 (en) 2002-01-04 2005-02-15 Becton, Dickinson And Company Binding protein as biosensors
DE60326256D1 (de) 2002-10-16 2009-04-02 Univ Duke Biosensor zum nachweis von glukose
US20050112685A1 (en) 2003-11-26 2005-05-26 Amiss Terry J. Compositions and methods for measuring analyte concentrations

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006044611A2 (en) * 2004-10-14 2006-04-27 Carnegie Institution Of Washington Neurotransmitter sensors and methods of using the same
WO2006096213A1 (en) * 2005-03-03 2006-09-14 Carnegie Institution Of Washington Polyamine sensors and methods of using the same
WO2006096214A1 (en) * 2005-03-04 2006-09-14 Carnegie Institution Of Washington Environmentally stable sensors and methods of using the same
WO2007046786A2 (en) * 2005-10-14 2007-04-26 Carnegie Institution Of Washington Sucrose biosensors and methods of using the same

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ANDERLUH GREGOR ET AL: "Concerted folding and binding of a flexible colicin domain to its periplasmic receptor TolA.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 24, 13 June 2003 (2003-06-13), pages 21860 - 21868, XP002553843, ISSN: 0021-9258 *
DEUSCHLE K ET AL: "Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering", PROTEIN SCIENCE, CAMBRIDGE UNIVERSITY PRESS, CAMBRIDGE, GB, vol. 14, no. 9, 1 September 2005 (2005-09-01), pages 2304 - 2314, XP003015644, ISSN: 0961-8368 *
FEHR MARCUS ET AL: "In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7 cells by fluorescent nanosensors.", THE JOURNAL OF BIOLOGICAL CHEMISTRY 23 MAY 2003, vol. 278, no. 21, 23 May 2003 (2003-05-23), pages 19127 - 19133, XP002553883, ISSN: 0021-9258 *
GE X ET AL: "Dual-Labeled Glucose Binding Protein for Ratiometric Measurements of Glucose", ANALYTICAL CHEMISTRY 20040301 US, vol. 76, no. 5, 1 March 2004 (2004-03-01), pages 1403 - 1410, XP002553841, ISSN: 0003-2700 *
HIRES SAMUEL ANDREW ET AL: "Optical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 18 MAR 2008, vol. 105, no. 11, 18 March 2008 (2008-03-18), pages 4411 - 4416, XP002553844, ISSN: 1091-6490 *
HOSSAIN MOHAMMED AKHTER ET AL: "Fluorescence resonance energy transfer in a novel cyclodextrin-peptide conjugate for detecting steroid molecules.", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 15 DEC 2003, vol. 13, no. 24, 15 December 2003 (2003-12-15), pages 4305 - 4308, XP002553842, ISSN: 0960-894X *
OKUMOTO SAKIKO ET AL: "Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 102, no. 24, 14 June 2005 (2005-06-14), pages 8740 - 8745, XP002481303, ISSN: 0027-8424 *
See also references of WO2006044612A2 *

Also Published As

Publication number Publication date
US20140323692A1 (en) 2014-10-30
CA2584108A1 (en) 2006-04-27
JP2008516607A (ja) 2008-05-22
JP5323356B2 (ja) 2013-10-23
US20170059565A1 (en) 2017-03-02
US20100037329A1 (en) 2010-02-11
IL183146A0 (en) 2009-02-11
US8530633B2 (en) 2013-09-10
AU2005295656B2 (en) 2011-08-04
EP1815246A2 (de) 2007-08-08
WO2006044612A2 (en) 2006-04-27
AU2005295656A1 (en) 2006-04-27
WO2006044612A3 (en) 2008-11-27

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