EP1614466A3 - System and method to prevent air bubbles in a hybridization chamber - Google Patents

System and method to prevent air bubbles in a hybridization chamber Download PDF

Info

Publication number
EP1614466A3
EP1614466A3 EP05105498A EP05105498A EP1614466A3 EP 1614466 A3 EP1614466 A3 EP 1614466A3 EP 05105498 A EP05105498 A EP 05105498A EP 05105498 A EP05105498 A EP 05105498A EP 1614466 A3 EP1614466 A3 EP 1614466A3
Authority
EP
European Patent Office
Prior art keywords
system
hybridization
pressure
chamber
slides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP05105498A
Other languages
German (de)
French (fr)
Other versions
EP1614466A2 (en
Inventor
Wolfgang Streit
Gyoergy Wenczel
Waltraud Lamprecht
Heribert Egelauer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tecan Trading AG
Original Assignee
Tecan Trading AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CH11442004 priority Critical
Priority to DE200420012163 priority patent/DE202004012163U1/en
Priority to US10/931,432 priority patent/US7615370B2/en
Application filed by Tecan Trading AG filed Critical Tecan Trading AG
Priority claimed from EP10172196.7A external-priority patent/EP2255880B1/en
Publication of EP1614466A2 publication Critical patent/EP1614466A2/en
Publication of EP1614466A3 publication Critical patent/EP1614466A3/en
Application status is Ceased legal-status Critical

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F11/00Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F11/0071Mixers with shaking, oscillating, or vibrating mechanisms the material being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F13/00Other mixers; Mixing plant, including combinations of mixers, e.g. of dissimilar mixers
    • B01F13/0059Micromixers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14Process control and prevention of errors
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0627Sensor or part of a sensor is integrated
    • B01L2300/0636Integrated biosensor, microarrays
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0822Slides
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877Flow chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/14Means for pressure control
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Abstract

The invention relates to a system (1) having hybridization chambers (5) for hybridizing nucleic acid samples, proteins or tissue sections immobilized on slides (27), each hybridization chamber (5) being defined as a substantially gap-shaped, substantially liquid-filled space between one of these Slides (27) and a lid (26) is defined, wherein the lid (26) is disposed opposite to the slide (27), that the hybridization chamber (5) is sealed from the ambient air, and wherein the system (1) an agitation device (32), with which liquids in the hybridization chambers (5) can be moved relative to the samples immobilized on the slides (27). The inventive system is characterized in that it comprises means for preventing air bubbles in the Hybridisierkammern (5), which is designed as a pressure device for establishing a chamber pressure in the Hybridisierkammern (5), said chamber pressure thereby independent of Agitationsdruckunterschieden above the normal atmospheric pressure prevailing in the ambient air, the system (1) comprises a spring member which communicates with the volume of liquid used for the hybridization and resiliently differentiates the agitation pressure applied by the agitation means (32) to the pressure space (34) counteracts.
EP05105498A 2004-07-08 2005-06-21 System and method to prevent air bubbles in a hybridization chamber Ceased EP1614466A3 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CH11442004 2004-07-08
DE200420012163 DE202004012163U1 (en) 2004-07-08 2004-08-04 System for hybridizing immobilized nucleic acid samples, in sealed hybridizing chambers, has a pressure assembly to build up the chamber pressures over ambient atmospheric levels to prevent the formation of air bubbles
US10/931,432 US7615370B2 (en) 2004-07-08 2004-09-01 System having device for preventing air bubbles in a hybridization chamber and corresponding method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP10172196.7A EP2255880B1 (en) 2005-06-21 2005-06-21 System and method to prevent air bubbles in a hybridization chamber

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP10172196.7A Division EP2255880B1 (en) 2004-07-08 2005-06-21 System and method to prevent air bubbles in a hybridization chamber

Publications (2)

Publication Number Publication Date
EP1614466A2 EP1614466A2 (en) 2006-01-11
EP1614466A3 true EP1614466A3 (en) 2006-05-10

Family

ID=35106752

Family Applications (1)

Application Number Title Priority Date Filing Date
EP05105498A Ceased EP1614466A3 (en) 2004-07-08 2005-06-21 System and method to prevent air bubbles in a hybridization chamber

Country Status (2)

Country Link
EP (1) EP1614466A3 (en)
JP (1) JP4819421B2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6190822B2 (en) * 2012-01-09 2017-08-30 マイクロニクス, インコーポレイテッド Microfluidic reactor system
US20080241844A1 (en) * 2007-02-06 2008-10-02 Network Biosystems, Inc. Devices and Methods for the Performance of Miniaturized In Vitro Assays
WO2008124116A1 (en) 2007-04-04 2008-10-16 Network Biosystems, Inc. Methods for rapid multiplexed amplification of target nucleic acids
CH699853A1 (en) 2008-11-13 2010-05-14 Tecan Trading Ag Meter and method for determining provided by a laboratory fluid system parameters.
WO2010147654A2 (en) 2009-06-15 2010-12-23 Netbio Inc. Improved methods for forensic dna quantitation
AT508703B1 (en) * 2009-07-15 2011-06-15 Ait Austrian Inst Technology Sample carrier
CN102740976B (en) 2010-01-29 2016-04-20 精密公司 Sampling-response microfluidic cartridge
CN104919035B (en) 2012-12-21 2017-08-11 精密公司 Portable fluorescence detecting system and micro- determination box
US10518262B2 (en) 2012-12-21 2019-12-31 Perkinelmer Health Sciences, Inc. Low elasticity films for microfluidic use
KR20150099816A (en) 2012-12-21 2015-09-01 마이크로닉스 인코포레이티드. Fluidic circuits and related manufacturing methods
EP2994543B1 (en) 2013-05-07 2018-08-15 Micronics, Inc. Device for preparation and analysis of nucleic acids
WO2014182831A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
US10386377B2 (en) 2013-05-07 2019-08-20 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
CN108431199A (en) * 2016-01-05 2018-08-21 日本板硝子株式会社 Reaction treating device, reaction treatment container, reaction treatment method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020009015A1 (en) * 1998-10-28 2002-01-24 Laugharn James A. Method and apparatus for acoustically controlling liquid solutions in microfluidic devices
US20020075363A1 (en) * 2000-05-15 2002-06-20 Mcneely Michael R. Air flow regulation in microfluidic circuits for pressure control and gaseous exchange
EP1260265A1 (en) * 2001-05-25 2002-11-27 Tecan Trading AG Apparatus for the preparation of a hybridisation chamber, processing set and system for the hybridisation of samples of nucleic acid, proteines and tissues
WO2004052527A1 (en) * 2002-12-11 2004-06-24 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Method and reactor for the amplification of dna

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6684966B2 (en) * 2001-10-18 2004-02-03 Baker Hughes Incorporated PCD face seal for earth-boring bit

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020009015A1 (en) * 1998-10-28 2002-01-24 Laugharn James A. Method and apparatus for acoustically controlling liquid solutions in microfluidic devices
US20020075363A1 (en) * 2000-05-15 2002-06-20 Mcneely Michael R. Air flow regulation in microfluidic circuits for pressure control and gaseous exchange
EP1260265A1 (en) * 2001-05-25 2002-11-27 Tecan Trading AG Apparatus for the preparation of a hybridisation chamber, processing set and system for the hybridisation of samples of nucleic acid, proteines and tissues
WO2004052527A1 (en) * 2002-12-11 2004-06-24 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Method and reactor for the amplification of dna

Also Published As

Publication number Publication date
JP2006084459A (en) 2006-03-30
EP1614466A2 (en) 2006-01-11
JP4819421B2 (en) 2011-11-24

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