EP2255880A3 - System and method to prevent air bubbles in a hybridization chamber - Google Patents

System and method to prevent air bubbles in a hybridization chamber Download PDF

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Publication number
EP2255880A3
EP2255880A3 EP10172196A EP10172196A EP2255880A3 EP 2255880 A3 EP2255880 A3 EP 2255880A3 EP 10172196 A EP10172196 A EP 10172196A EP 10172196 A EP10172196 A EP 10172196A EP 2255880 A3 EP2255880 A3 EP 2255880A3
Authority
EP
European Patent Office
Prior art keywords
chamber
hybridization chamber
air bubbles
hybridization
hybridisierkammer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP10172196A
Other languages
German (de)
French (fr)
Other versions
EP2255880B1 (en
EP2255880A2 (en
Inventor
Wolfgang Streit
Gyoergy Wenczel
Waltraud Lambrecht
Heribert Eglauer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tecan Trading AG
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Tecan Trading AG
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Filing date
Publication date
Application filed by Tecan Trading AG filed Critical Tecan Trading AG
Priority claimed from EP05105498A external-priority patent/EP1614466A3/en
Publication of EP2255880A2 publication Critical patent/EP2255880A2/en
Publication of EP2255880A3 publication Critical patent/EP2255880A3/en
Application granted granted Critical
Publication of EP2255880B1 publication Critical patent/EP2255880B1/en
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/65—Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Abstract

Die Erfindung betrifft ein Verfahren zum Verhindern von Luftblasen in einer im wesentlichen spaltförmigen Hybridisierkammer (5) eines Systems (1) zum Hybridisieren von auf Objektträgern (27) immobilisierten Nukleinsäureproben, Proteinen oder Gewebeschnitten. Dabei umfasst das Verfahren das Bereitstellen einer im Wesentlichen spaltförmigen Hybridisierkammer (5) zwischen einem Objektträger (27) und einem Deckel (26) (wobei der Deckel (26) derart gegenüber dem Objektträger (27) angeordnet wird, dass die Hybridisierkammer (5) gegenüber der Umgebungsluft abgedichtet ist) und das im wesentlichen Füllen der bereitgestellten Hybridisierkammer (5) mit einer Flüssigkeit. Das erfindungsgemässe Verfahren ist dadurch gekennzeichnet, dass es zudem das Ausbilden eines Kammer-Drucks in der Hybridisierkammer (5) mit einer Druckeinrichtung dieses Systems (1) und das Aufrechterhalten dieses Kammer-Drucks während eines Hybridisiervorgangs auf einem Wert, der mindestens 100 mbar über dem in der Umgebungsluft herrschenden, normalen atmosphärischen Druck liegt, umfasst.

Figure imgaf001
The invention relates to a method for preventing air bubbles in a substantially slit-shaped hybridization chamber (5) of a system (1) for hybridizing nucleic acid samples, proteins or tissue sections immobilized on slides (27). The method comprises providing a substantially gap-shaped hybridization chamber (5) between a slide (27) and a lid (26) (wherein the lid (26) is positioned opposite the slide (27) such that the hybridization chamber (5) faces the ambient air is sealed) and substantially filling the provided Hybridisierkammer (5) with a liquid. The inventive method is characterized in that it further comprises forming a chamber pressure in the Hybridisierkammer (5) with a pressure device of this system (1) and maintaining this chamber pressure during a hybridization process at a value which is at least 100 mbar above the ambient atmospheric pressure prevails.
Figure imgaf001
EP10172196.7A 2005-06-21 2005-06-21 System and method to prevent air bubbles in a hybridization chamber Not-in-force EP2255880B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP05105498A EP1614466A3 (en) 2004-07-08 2005-06-21 System and method to prevent air bubbles in a hybridization chamber

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP05105498A Division EP1614466A3 (en) 2004-07-08 2005-06-21 System and method to prevent air bubbles in a hybridization chamber
EP05105498.9 Division 2005-06-21

Publications (3)

Publication Number Publication Date
EP2255880A2 EP2255880A2 (en) 2010-12-01
EP2255880A3 true EP2255880A3 (en) 2011-08-17
EP2255880B1 EP2255880B1 (en) 2014-05-28

Family

ID=42989350

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10172196.7A Not-in-force EP2255880B1 (en) 2005-06-21 2005-06-21 System and method to prevent air bubbles in a hybridization chamber

Country Status (1)

Country Link
EP (1) EP2255880B1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9535082B2 (en) 2013-03-13 2017-01-03 Abbott Laboratories Methods and apparatus to agitate a liquid
US10058866B2 (en) 2013-03-13 2018-08-28 Abbott Laboratories Methods and apparatus to mitigate bubble formation in a liquid
USD962471S1 (en) 2013-03-13 2022-08-30 Abbott Laboratories Reagent container
USD978375S1 (en) 2013-03-13 2023-02-14 Abbott Laboratories Reagent container
US10512911B1 (en) * 2018-12-07 2019-12-24 Ultima Genomics, Inc. Implementing barriers for controlled environments during sample processing and detection
CN111111481B (en) * 2020-01-19 2024-01-23 防灾科技学院 System and method for manufacturing trace dissolved ternary mixed gas standard solution

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6258534B1 (en) * 1998-03-02 2001-07-10 Bbi Bioseq, Inc. Pressure-controlled nucleic acid hybridization
US20020009015A1 (en) * 1998-10-28 2002-01-24 Laugharn James A. Method and apparatus for acoustically controlling liquid solutions in microfluidic devices
US20020075363A1 (en) * 2000-05-15 2002-06-20 Mcneely Michael R. Air flow regulation in microfluidic circuits for pressure control and gaseous exchange
EP1260265A1 (en) * 2001-05-25 2002-11-27 Tecan Trading AG Apparatus for the preparation of a hybridisation chamber, processing set and system for the hybridisation of samples of nucleic acid, proteines and tissues
WO2004052527A1 (en) * 2002-12-11 2004-06-24 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Method and reactor for the amplification of dna

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
WO2000009650A1 (en) 1998-08-10 2000-02-24 Genomic Solutions, Inc. A thermal/fluidic cycling device for the purpose of nucleic acid hybridization
US6186659B1 (en) 1998-08-21 2001-02-13 Agilent Technologies Inc. Apparatus and method for mixing a film of fluid
US6458526B1 (en) 2000-01-28 2002-10-01 Agilent Technologies, Inc. Method and apparatus to inhibit bubble formation in a fluid

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6258534B1 (en) * 1998-03-02 2001-07-10 Bbi Bioseq, Inc. Pressure-controlled nucleic acid hybridization
US20020009015A1 (en) * 1998-10-28 2002-01-24 Laugharn James A. Method and apparatus for acoustically controlling liquid solutions in microfluidic devices
US20020075363A1 (en) * 2000-05-15 2002-06-20 Mcneely Michael R. Air flow regulation in microfluidic circuits for pressure control and gaseous exchange
EP1260265A1 (en) * 2001-05-25 2002-11-27 Tecan Trading AG Apparatus for the preparation of a hybridisation chamber, processing set and system for the hybridisation of samples of nucleic acid, proteines and tissues
WO2004052527A1 (en) * 2002-12-11 2004-06-24 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Method and reactor for the amplification of dna

Also Published As

Publication number Publication date
EP2255880B1 (en) 2014-05-28
EP2255880A2 (en) 2010-12-01

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