EP1563073A4 - Proteinproduktionsverfahren und modifizierte zellen zur verwendung darin - Google Patents
Proteinproduktionsverfahren und modifizierte zellen zur verwendung darinInfo
- Publication number
- EP1563073A4 EP1563073A4 EP03739320A EP03739320A EP1563073A4 EP 1563073 A4 EP1563073 A4 EP 1563073A4 EP 03739320 A EP03739320 A EP 03739320A EP 03739320 A EP03739320 A EP 03739320A EP 1563073 A4 EP1563073 A4 EP 1563073A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- production methods
- protein production
- modified cells
- cells
- modified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4747—Apoptosis related proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/36—Lipids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/48—Regulators of apoptosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Cell Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US39173802P | 2002-06-26 | 2002-06-26 | |
US391738P | 2002-06-26 | ||
US44049803P | 2003-01-16 | 2003-01-16 | |
US440498P | 2003-01-16 | ||
PCT/US2003/020207 WO2004003151A2 (en) | 2002-06-26 | 2003-06-26 | Protein production methods and modified cells for use therein |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1563073A2 EP1563073A2 (de) | 2005-08-17 |
EP1563073A4 true EP1563073A4 (de) | 2007-09-19 |
Family
ID=30003205
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP03739320A Withdrawn EP1563073A4 (de) | 2002-06-26 | 2003-06-26 | Proteinproduktionsverfahren und modifizierte zellen zur verwendung darin |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1563073A4 (de) |
JP (2) | JP2006503555A (de) |
AU (2) | AU2003245702A1 (de) |
CA (1) | CA2491212A1 (de) |
WO (1) | WO2004003151A2 (de) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100454016B1 (ko) * | 2002-01-05 | 2004-10-26 | 한국과학기술원 | 항예정사 유전자로 형질전환되고 dhfr 유전자가결핍된 신규한 cho 세포주, 그의 제조 방법 및 상기형질전환된 cho 숙주 세포를 이용한 목적단백질의 생산방법 |
US7531327B2 (en) * | 2004-07-23 | 2009-05-12 | Immunomedics, Inc. | Methods and compositions for increasing longevity and protein yield from a cell culture |
EP2209891A1 (de) * | 2007-11-13 | 2010-07-28 | Boehringer Ingelheim Pharma GmbH & Co. KG | Verbesserung des sekretionsvermögens bei wirtszellen |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1348758A1 (de) * | 2002-03-28 | 2003-10-01 | Boehringer Ingelheim Pharma GmbH & Co.KG | Wirtszellen mit verbessertem Überleben und Verfahren zur Herstellung von diesen Zellen |
EP1468079A1 (de) * | 2002-01-05 | 2004-10-20 | Korea Advanced Institute of Science and Technology | Mit einem antiapoptotischem gen transfizierte dhfr-defiziente cho-zellinie, verfahren zur herstellung davon und verfahren zur produktion von zielprotein unter verwendung davon |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU6163196A (en) * | 1995-06-07 | 1996-12-30 | Smithkline Beecham Corporation | Method for obtaining receptor agonist antibodies |
US6218181B1 (en) * | 1998-03-18 | 2001-04-17 | The Salk Institute For Biological Studies | Retroviral packaging cell line |
-
2003
- 2003-06-26 AU AU2003245702A patent/AU2003245702A1/en not_active Abandoned
- 2003-06-26 WO PCT/US2003/020207 patent/WO2004003151A2/en active Search and Examination
- 2003-06-26 CA CA002491212A patent/CA2491212A1/en not_active Abandoned
- 2003-06-26 JP JP2004517898A patent/JP2006503555A/ja not_active Withdrawn
- 2003-06-26 EP EP03739320A patent/EP1563073A4/de not_active Withdrawn
-
2010
- 2010-03-29 AU AU2010201256A patent/AU2010201256A1/en not_active Abandoned
- 2010-10-15 JP JP2010233087A patent/JP2011004771A/ja not_active Ceased
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1468079A1 (de) * | 2002-01-05 | 2004-10-20 | Korea Advanced Institute of Science and Technology | Mit einem antiapoptotischem gen transfizierte dhfr-defiziente cho-zellinie, verfahren zur herstellung davon und verfahren zur produktion von zielprotein unter verwendung davon |
EP1348758A1 (de) * | 2002-03-28 | 2003-10-01 | Boehringer Ingelheim Pharma GmbH & Co.KG | Wirtszellen mit verbessertem Überleben und Verfahren zur Herstellung von diesen Zellen |
Non-Patent Citations (12)
Title |
---|
CHIANG GISELA G ET AL: "Bcl-x(L) mediates increased production of humanized monoclonal antibodies in chinese hamster ovary cells", BIOTECHNOLOGY AND BIOENGINEERING, vol. 91, no. 7, September 2005 (2005-09-01), pages 779 - 792, XP002446137, ISSN: 0006-3592 * |
FIGUEROA B ET AL: "A COMPARISON OF THE PROPERTIES OF A BCL-XL VARIANT TO THE WILD-TYPE ANTI-APOPTOSIS INHIBITOR IN MAMMALIAN CELL CULTURES", METABOLIC ENGINEERING, ACADEMIC PRESS,, US, vol. 5, no. 4, October 2003 (2003-10-01), pages 230 - 245, XP008064154, ISSN: 1096-7176 * |
FUSSENEGGER M ET AL: "CONTROLLED PROLIFERATION BY MULTIGENE METABOLIC ENGINEERING ENHANCES THE PRODUCTIVITY OF CHINESE HAMSTER OVARY CELLS", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 16, no. 5, May 1998 (1998-05-01), pages 468 - 472, XP001093788, ISSN: 1087-0156 * |
FUSSENEGGER M. ET AL: "Regulated overexpression of the survival factor bcl-2 in CHO cells increases viable cell density in batch culture and decreases DNA release in extended fixed-bed cultivation", CYTOTECHNOLOGY, vol. 32, no. 1, 1 January 2000 (2000-01-01), KLUWER ACADEMIC PUBLISHERS, DORDRECHT, NL, pages 45 - 61, XP002326419 * |
GOSWAMI J ET AL: "Apoptosis in batch cultures of Chinese hamster ovary cells", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, vol. 62, no. 6, 20 March 1999 (1999-03-20), pages 632 - 640, XP002326418, ISSN: 0006-3592 * |
JOEL CHARBONNEAU ET AL: "Protection of hybridoma cells against apoptosis by a loop domain-deficient Bcl-xL protein", CYTOTECHNOLOGY, KLUWER ACADEMIC PUBLISHERS, DO, vol. 37, no. 1, 1 September 2001 (2001-09-01), pages 41 - 47, XP019236711, ISSN: 1573-0778 * |
JUNG D. ET AL: "Inducible expression of Bcl-XL restricts apoptosis resistance to the antibody secretion phase in hybridoma cultures", BIOTECHNOLOGY AND BIOENGINEERING, vol. 79, no. 2, 21 May 2002 (2002-05-21) - 20 July 2002 (2002-07-20), pages 180 - 187 * |
KIM N S ET AL: "Overexpression of bcl-2 inhibits sodium butyrate-induced apoptosis in Chinese hamster ovary cells resulting in enhanced humanized antibody production", BIOTECHNOLOGY AND BIOENGINEERING - COMBINATORIAL CHEMISTRY, WILEY, NEW YORK, NY, US, vol. 71, no. 3, 2000, pages 184 - 193, XP002208594 * |
MASTRANGELO A J ET AL: "Part II. Overexpression of bcl-2 family members enhances survival of mammalian cells in response to various culture insults", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, vol. 67, no. 5, 5 March 2000 (2000-03-05), pages 555 - 564, XP002326420, ISSN: 0006-3592 * |
MASTRANGELO ALISON J ET AL: "Part I. Bcl-2 and bcl-xL limit apoptosis upon infection with alphavirus vectors", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, vol. 67, no. 5, 5 March 2000 (2000-03-05), pages 544 - 554, XP002217633, ISSN: 0006-3592 * |
MEENTS H ET AL: "Impact of coexpression and coamplification of sICAM and antiapoptosis determinants bcl-2/bcl-x(L) on productivity, cell survival, and mitochondria number in CHO-DG44 grown in suspension and serum-free media", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, vol. 80, no. 6, 20 December 2002 (2002-12-20), pages 706 - 716, XP002251640, ISSN: 0006-3592 * |
ZANGHI JAMES A ET AL: "Serum protects protein-free competent Chinese hamster ovary cells against apoptosis induced by nutrient deprivation in batch culture", BIOTECHNOLOGY AND BIOENGINEERING, vol. 64, no. 1, 5 July 1999 (1999-07-05), pages 108 - 119, XP002446136, ISSN: 0006-3592 * |
Also Published As
Publication number | Publication date |
---|---|
WO2004003151A2 (en) | 2004-01-08 |
JP2006503555A (ja) | 2006-02-02 |
AU2010201256A1 (en) | 2010-04-22 |
CA2491212A1 (en) | 2004-01-08 |
EP1563073A2 (de) | 2005-08-17 |
JP2011004771A (ja) | 2011-01-13 |
WO2004003151A3 (en) | 2005-06-23 |
AU2003245702A1 (en) | 2004-01-19 |
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Legal Events
Date | Code | Title | Description |
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PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
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AX | Request for extension of the european patent |
Extension state: AL LT LV MK |
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DAX | Request for extension of the european patent (deleted) | ||
REG | Reference to a national code |
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A4 | Supplementary search report drawn up and despatched |
Effective date: 20070822 |
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17Q | First examination report despatched |
Effective date: 20071213 |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN |
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