WO2004003151A3 - Protein production methods and modified cells for use therein - Google Patents

Protein production methods and modified cells for use therein Download PDF

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Publication number
WO2004003151A3
WO2004003151A3 PCT/US2003/020207 US0320207W WO2004003151A3 WO 2004003151 A3 WO2004003151 A3 WO 2004003151A3 US 0320207 W US0320207 W US 0320207W WO 2004003151 A3 WO2004003151 A3 WO 2004003151A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein production
production methods
modified cells
protein
cells
Prior art date
Application number
PCT/US2003/020207
Other languages
French (fr)
Other versions
WO2004003151A2 (en
Inventor
Gisela Chiang
William Sisk
Original Assignee
Biogen Idec Inc
Gisela Chiang
William Sisk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogen Idec Inc, Gisela Chiang, William Sisk filed Critical Biogen Idec Inc
Priority to AU2003245702A priority Critical patent/AU2003245702A1/en
Priority to EP03739320A priority patent/EP1563073A4/en
Priority to JP2004517898A priority patent/JP2006503555A/en
Priority to CA002491212A priority patent/CA2491212A1/en
Publication of WO2004003151A2 publication Critical patent/WO2004003151A2/en
Publication of WO2004003151A3 publication Critical patent/WO2004003151A3/en
Priority to AU2010201256A priority patent/AU2010201256A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4747—Apoptosis related proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/48—Regulators of apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Disclosed are cells having an increased amount of Bcl-xL protein and their use in protein production.
PCT/US2003/020207 2002-06-26 2003-06-26 Protein production methods and modified cells for use therein WO2004003151A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2003245702A AU2003245702A1 (en) 2002-06-26 2003-06-26 Protein production methods and modified cells for use therein
EP03739320A EP1563073A4 (en) 2002-06-26 2003-06-26 Protein production methods and modified cells for use therein
JP2004517898A JP2006503555A (en) 2002-06-26 2003-06-26 Protein production methods and modified cells for use
CA002491212A CA2491212A1 (en) 2002-06-26 2003-06-26 Protein production methods and modified cells for use therein
AU2010201256A AU2010201256A1 (en) 2002-06-26 2010-03-29 Protein Production Methods and Modified Cells for Use Therein

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US39173802P 2002-06-26 2002-06-26
US60/391,738 2002-06-26
US44049803P 2003-01-16 2003-01-16
US60/440,498 2003-01-16

Publications (2)

Publication Number Publication Date
WO2004003151A2 WO2004003151A2 (en) 2004-01-08
WO2004003151A3 true WO2004003151A3 (en) 2005-06-23

Family

ID=30003205

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/020207 WO2004003151A2 (en) 2002-06-26 2003-06-26 Protein production methods and modified cells for use therein

Country Status (5)

Country Link
EP (1) EP1563073A4 (en)
JP (2) JP2006503555A (en)
AU (2) AU2003245702A1 (en)
CA (1) CA2491212A1 (en)
WO (1) WO2004003151A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100454016B1 (en) * 2002-01-05 2004-10-26 한국과학기술원 Novel DHFR-deficient CHO cell line transfected with anti-apoptotic gene, method for preparation thereof and method for production of target proteins using the same
US7531327B2 (en) * 2004-07-23 2009-05-12 Immunomedics, Inc. Methods and compositions for increasing longevity and protein yield from a cell culture
US20110281301A1 (en) * 2007-11-13 2011-11-17 Boehringer Ingelheim Pharma Gmbh & Co. Kg The secretory capacity in host cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153190A (en) * 1995-06-07 2000-11-28 Young; Peter Ronald Erythropoietin receptor antibodies
US6218181B1 (en) * 1998-03-18 2001-04-17 The Salk Institute For Biological Studies Retroviral packaging cell line

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100454016B1 (en) * 2002-01-05 2004-10-26 한국과학기술원 Novel DHFR-deficient CHO cell line transfected with anti-apoptotic gene, method for preparation thereof and method for production of target proteins using the same
EP1348758A1 (en) * 2002-03-28 2003-10-01 Boehringer Ingelheim Pharma GmbH & Co.KG Host cells having improved cell survival properties and methods to generate such cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153190A (en) * 1995-06-07 2000-11-28 Young; Peter Ronald Erythropoietin receptor antibodies
US6218181B1 (en) * 1998-03-18 2001-04-17 The Salk Institute For Biological Studies Retroviral packaging cell line

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GONZALES-GARCIA ET AL.: "Bcl-XL is the major bcl-x mRNA form expressed during murine development and its product localizes to mitochondria", DEVELOPMENT, vol. 120, 1994, pages 3033 - 3042, XP002933985 *
INOHARA ET AL.: "Mtd, a novel Bcl-2 family member activates apoptosis in the absense of heterodimerization with Bcl-2 and Bcl-XL", J. BIOL. CHEM., vol. 273, no. 15, 1998, pages 8705 - 8710, XP002233023 *
See also references of EP1563073A4 *

Also Published As

Publication number Publication date
CA2491212A1 (en) 2004-01-08
JP2011004771A (en) 2011-01-13
EP1563073A4 (en) 2007-09-19
EP1563073A2 (en) 2005-08-17
WO2004003151A2 (en) 2004-01-08
AU2010201256A1 (en) 2010-04-22
AU2003245702A1 (en) 2004-01-19
JP2006503555A (en) 2006-02-02

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