EP1556501A1 - Dosage transdermique a l'aide d'un dispositif de blocage magnetique - Google Patents

Dosage transdermique a l'aide d'un dispositif de blocage magnetique

Info

Publication number
EP1556501A1
EP1556501A1 EP03777948A EP03777948A EP1556501A1 EP 1556501 A1 EP1556501 A1 EP 1556501A1 EP 03777948 A EP03777948 A EP 03777948A EP 03777948 A EP03777948 A EP 03777948A EP 1556501 A1 EP1556501 A1 EP 1556501A1
Authority
EP
European Patent Office
Prior art keywords
sample
transdermal
tissue
transdermal assay
magnetic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03777948A
Other languages
German (de)
English (en)
Other versions
EP1556501A4 (fr
Inventor
Michael J. Cima
Hongming Chen
J. Richard Gyory
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transform Pharmaceuticals Inc
Original Assignee
Transform Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/282,505 external-priority patent/US6852526B2/en
Application filed by Transform Pharmaceuticals Inc filed Critical Transform Pharmaceuticals Inc
Publication of EP1556501A1 publication Critical patent/EP1556501A1/fr
Publication of EP1556501A4 publication Critical patent/EP1556501A4/fr
Withdrawn legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/08—Investigating permeability, pore-volume, or surface area of porous materials
    • G01N15/0806—Details, e.g. sample holders, mounting samples for testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B01L2300/0618—Holders integrated in container to position an object for removable separation walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates

Definitions

  • the field of the present invention relates to tissue barrier assays for screening formulations and chemical compositions.
  • tissue barrier assay In vitro analysis of the movement of compounds (e.g., drugs) across an epithelial barrier, such as intestinal epithelium or airway epithelium, is typically performed using an Ussing-type chamber.
  • a tissue barrier assay using an Ussing-type chamber, a piece tissue is removed as an intact sheet from the body and mounted in a device which contains an enclosed, internal hollow chamber such that it divides the internal chamber into two separate chambers. Thereafter, biologically compatible solutions are filled into both chambers, and the drug of interest is added to one chamber's solution. Samples are then removed from the contralateral chamber solution at various times to determine the rate at which the drug moves across the tissue barrier.
  • This type of tissue barrier assay is cumbersome, inefficient, and only permits a very limited number of independent samples to be derived from a unit area of tissue sheet.
  • Transdermal delivery of drugs is a type of tissue transfer that involves transfer of the drug from a transdermal drug delivery device through the skin and into the patient's blood stream.
  • Transdermal drug delivery offers many advantages compared to other methods of drug delivery.
  • One obvious advantage is that needles and the associated pain are avoided. This is especially desirable for drugs that are repeatedly administered. Avoiding the unpleasantness of needles would also lead to improved patient compliance of drug regimens.
  • Another advantage of transdermal drug delivery is its ability to offer prolonged or sustained delivery, potentially over several days to weeks. Other delivery methods, such as oral or pulmonary delivery, typically require that the drug be given repeatedly to sustain the proper concentration of drug within the body. With sustained transdermal delivery, dose maintenance is performed automatically over a long period of time.
  • a final advantage is that drug molecules only have to cross the skin to reach the bloodstream when given transdermally.
  • Transdermally administered drugs bypass first- pass metabolism in the liver, and also avoid other degradation pathways such as the low pH's and enzymes present in the gastrointestinal tract.
  • the skin is the largest organ of the body. It is highly impermeable to prevent loss of water and electrolytes. It is subdivided into two main layers: the outer epidermis and the inner dermis.
  • the epidermis is the outer layer of the skin, 50 to 100 micron thick (Monteiro-Riviere, 1991; Champion, et al., 1992).
  • the dermis is the inner layer of the skin and varies from 1 to 3 mm in thickness.
  • the goal of transdermal drug delivery is to get the drug to this layer of the skin, where the blood capillaries are located, to allow the drug to be systemically delivered.
  • the epidermis does not contain nerve endings or blood vessels.
  • the main purpose of the epidermis is to generate a tough layer of dead cells on the surface of the skin, thereby protecting the body from the environment.
  • This outermost layer of epidermis is called the stratum corneum, and the dead cells that comprise it are called comeocytes or keratinocytes.
  • stratum corneum is commonly modeled or described as a brick wall
  • the "bricks” are the flattened, dead comeocytes. Typically, there are about 10 to 15 comeocytes stacked vertically across the stratum corneum (Monteiro- Riviere, 1991; Champion et al., 1992). The comeocytes are encased in sheets of lipid bilayers (the "mortar”). The lipid bilayer sheets are separated by ⁇ 50nm. Typically, there are about 4 to 8 lipid bilayers between each pair of comeocytes.
  • the lipid matrix is primarily composed of ceramides, sphingolipids, cholesterol, fatty acids, and sterols, with very little water present (Lampe et al, 1983 [a]; Lampe et al., 1983 [b]; Elias, 1988).
  • the stratum corneum is the primary barrier to the entry of molecules or microorganisms across the skin. Most molecules pass through the stratum comeum only with great difficulty, which is why the transdermal drag delivery route has not been more widely used to date. Once the molecules have crossed the stratum comeum, diffusion across the epidermis and dermis to the blood vessels occurs rapidly. Thus, most of the attention in transdermal drug delivery research has been focused on transporting molecules and drags across the stratum comeum.
  • transdermal drag delivery device The most common form of transdermal drag delivery device is the transdermal drag "patch," where a drug, or pharmaceutical, is contained within a reservoir placed next to the skin (Schaefer and Redelmeier, 1996).
  • the drug molecules typically cross the skin by simple diffusion. Transport is governed by the rate of molecular diffusion into and out of the skin, and partitioning of the drag into the skin.
  • transdermal drag delivery is limited to small, lipophilic molecules such as scopolamine, nitroglycerine, and nicotine, which readily permeate the skin.
  • the delivery is slow, typically taking hours for the drag to cross the skin, and treatment is only effective when a very small amount of drag is required to have a biological effect (Guy and Hadgraft, 1989).
  • Transdermal drug delivery devices such as a transdermal patch, also generally contain an adhesive, which serves to keep the device in intimate contact with the skin, and may also form the matrix in which the drug is dissolved or dispersed. There are many different forms of adhesives that can be used, and it is often a very difficult problem to select which adhesive to use with any drug or drug and enhancer.
  • the present invention relates to high-throughput systems and methods to prepare a large number of component combinations, at varying concentrations and identities, at the same time, and high-throughput methods to test tissue barrier transfer of components in each combination.
  • the methods of the present invention allow determination of the effects of additional or inactive components, such as excipients, carriers, enhancers, adhesives, and additives, on transfer of active components, such as pharmaceuticals, across tissue, such as skin, lung tissue, tracheal tissue, nasal tissue, bladder tissue, placenta, vaginal tissue, rectal tissue, stomach tissue, gastrointestinal tissue, and eye or corneal tissue.
  • the invention thus encompasses the high-throughput testing of pharmaceutical compositions or formulations in order to determine the overall optimal composition or formulation for improved tissue transport, including without limitation, transdermal transport. Specific embodiments of this invention are described in detail below.
  • the invention concerns an apparatus for measuring transfer of components across a tissue, comprising a support plate, an array of samples supported by the support plate, a membrane or tissue specimen overlaying the array of samples, and a reservoir plate secured to a side of the membrane or tissue specimen opposite the array of samples.
  • each sample in the array contains a unique composition or formulation of components, wherein different active components or different physical states of an active component are present in one or more of the samples in the sample array.
  • each sample of the array includes a component-in-common and at least one additional component, wherein each sample differs from at least one other sample with respect to at least one of:
  • a "component-in-common” is a component that is present in every sample in a sample array.
  • the component-in-common is an active component, and preferably, the active component is a pharmaceutical, dietary supplement, alternative medicine or a nutraceutical.
  • the samples may be in the form of liquids, solutions, suspensions, emulsions, solids, semi-solids, gels, foams, pastes, ointments, or triturates.
  • the invention concerns a method of measuring tissue barrier transport of a sample, comprising: [0024] (a) preparing an array of samples having an active component and at least one additional component, wherein each sample differs from at least one other sample with respect to at least one of: [0025] (i) the identity of the active component;
  • the active component is a pharmaceutical, a dietary supplement, an alternative medicine, or a nutraceutical.
  • the tissue specimen is skin.
  • the invention concerns a method of analyzing or measuring flux of a sample across a tissue, comprising: [0035] (a) preparing an array of samples having a component-in-common and at least one additional component, wherein each sample differs from at least one other sample with respect to at least one of: [0036] (i) the identity of an active component;
  • the method comprises an additional step of cutting the tissue specimen to avoid lateral diffusion between wells.
  • the method preferably comprises analyzing the tissue specimen for defects, or inhomogeneities, and correcting for or repairing the defects.
  • the transdermal assay apparatus includes at least first and second members.
  • the first member has one or more sample surfaces, each of which is configured to receive a sample , thereon.
  • the second member defines one or more reservoirs, each of which has an opening on a surface of the second member. Each sample surface is substantially the same size as each opening.
  • the transdermal assay apparatus also includes a magnetic clamp configured to clamp a tissue specimen between the sample surface and the opening. The magnetic clamp also seals the various members of the apparatus together.
  • the magnetic clamp preferably includes a magnet having a strength that is selected based on the clamping force required between the first member and the second member.
  • the invention also provides a method for using a transdermal assay apparatus.
  • a sample is placed on a sample surface of a first member.
  • a reservoir defined by a second member is filled with a fluid medium, where the reservoir has an opening on a surface of the second member.
  • a tissue specimen is then placed between the sample and the opening. The tissue specimen is subsequently clamped between the sample and the fluid medium at the opening, with a magnetic clamping force.
  • a specimen of the fluid medium is withdrawn from the reservoir to measure the concenration of the sample in the fluid medium. This concentration is indicative of a transfer of the sample across the tissue specimen.
  • the above described apparatus and method are preferably used for high- throughput screening of active component or drag flux through the stratum comeum recognizing that such flux is determined, at least in part, by the permeability of the drug within the tissue in the presence of an enhancer.
  • the permeability is generally governed by at least two factors: the solubility of the active component or drag within the stratum corneum and the diffusivity of the active component or drag within the stratum comeum. These two factors, solubility and diffusivity, can be measured independently as a method of indirectly assessing the flux through the stratum comeum.
  • an array of wells containing samples of different compositions of active component and inactive compounds including without limitation, compositions comprising active component/carrier or excipient/, active component/carrier or excipient/enhancer/, active component/adhesive/enhancer/additive, are constructed.
  • compositions comprising active component/carrier or excipient/, active component/carrier or excipient/enhancer/, active component/adhesive/enhancer/additive, are constructed.
  • Known amounts of stratum comeum are added to each well and the rate at which the active component or drag is taken up into the tissue sample is measured by extracting the tissue from similarly prepared wells at different times. Measuring the concentration after times sufficiently long so that the amount dissolved is not changing with time can assess the equilibrium concentration of active component or drug within the tissue.
  • the product of the rate and solubility is proportional to the permeability of the active component or drag.
  • the high-throughput combinatorial screening systems and methods of the present invention identify optimal compositions or formulations to achieve a desired result for such compositions or formulations, including without limitation, construction of a transdermal delivery device.
  • the systems and methods of the present invention may be used to identify 1) optimal compositions or formulations comprising one or more active components and one or more inactive components for achieving desired characteristics for such compositions or formulations, 2) optimal adhesive/enhancer/additive compositions for compatibility with a drug, 3) optimal drag/adhesive/enhancer/additive compositions for maximum drug flux through stratum comeum, and 4) optimal drag/adhesive/enhancer/additive composition to minimize cytotoxicity
  • FIG. 1 is a schematic diagram of a high-throughput apparatus for measuring tissue barrier transport, such as transdermal transport, according to the present invention
  • FIGS. 2 A - 2D are schematic diagrams of an alternative embodiment of a high-throughput apparatus for measuring tissue barrier transport using solid source samples according to the present invention
  • FIG. 3 is a schematic diagram of an alternative embodiment of a high- throughput apparatus for measuring tissue barrier transport according to the present invention
  • FIGS. 4 A - 4C are schematic diagrams of an alternative embodiment of a diffusion cell that alone, or as part of a high throughput apparatus, is used for measuring tissue barrier transport according to the present invention.
  • FIGS. 5A and 5B are schematic diagrams of an apparatus for filling a sample well in a sample array, such as the sample array in the high-throughput apparatus shown in FIG. 1;
  • FIG. 6 is a schematic diagram of an alternative embodiment of a high- throughput apparatus for measuring or analyzing tissue barrier transport using solid source samples according to the present invention
  • FIG. 7 is a schematic diagram of an alternative embodiment of a high- throughput apparatus for measuring or analyzing tissue barrier transport using solid source samples according to the present invention.
  • FIG. 8 is a schematic diagram of an alternative embodiment of a high- throughput apparatus for measuring or analyzing tissue barrier transport using solid source samples according to the present invention.
  • FIG. 9 is an exploded view of a another transdermal assay apparatus according to another embodiment of the invention.
  • FIG. 10 is an exploded view of a yet another transdermal assay apparatus according to yet another embodiment of the invention.
  • the present invention relates to high throughput combinatorial systems and methods that improve tissue barrier transfer of active compounds, such as pharmaceuticals or drugs, other compounds, or compound combinations.
  • the system and methods of the present invention maybe used to identify the optimal components (e.g., solvents, carriers, transport enhancers, adhesives, additives, and other excipients) for pharmaceutical compositions or formulations that are delivered to a patient via tissue transport, including without limitation, pharmaceutical compositions or formulations administered or delivered transdermally (e.g., in the form of a transdermal delivery device), topically (e.g., in the form of ointments, lotions, gels, and solutions), and ocularly (e.g., in the form of a solution).
  • "high throughput” refers to the number of samples generated or screened as described herein, typically at least 10, more typically at least 50 to
  • the high throughput methods of the present invention can be performed using various forms of samples. Typically, the methods are performed either with liquid samples or with solid or semi-solid samples.
  • liquid source means that the sample containing the component or components being measured or analyzed is in the form of a liquid, which includes, without limitation, liquids, solutions, emulsions, suspensions, and any of the foregoing having solid particulates dispersed therein.
  • solid source means that the sample containing the component or components being measured or analyzed is in the form of a solid or semi-solid, which includes, without limitation, triturates, gels, films, foams, pastes, ointments, adhesives, high viscoelastic liquids, high viscoelastic liquids having solid particulates dispersed therein, and transdermal patches.
  • reservoir medium refers to a liquid, solution, gel, or sponge that is chemically compatible with the components in a sample and the tissue being used in an apparatus or method of the present invention.
  • the reservoir medium comprises part of the specimen taken to measure or analyze the transfer, flux, or diffusion of a component across a tissue barrier.
  • the reservoir medium is a liquid or solution.
  • FIG. 1 shows a schematic diagram of a preferred embodiment of a high- throughput apparatus 100 for measuring tissue barrier transport in a sample array 112 according to the present invention.
  • Apparatus 100 includes a substrate plate 114 supporting sample array 112, a tissue specimen 120 and a reservoir plate 130.
  • each sample in sample array 112 is placed in a sample well 116.
  • Attached to the bottom of substrate plate 114 is a base 118 that forms the bottom of each sample well 116.
  • Base 118 is optionally a membrane made of any suitable material (e.g. a rubber membrane) in any fashion that permits air to bleed out of sample well 116 when filling with a sample.
  • base 118 is a rigid, removable substrate plate such as plate 214 (described infra with respect to FIGS. 2A-2D) capable of supporting an array of solid source samples.
  • Substrate plate 114 may be any rigid grid or plate capable of supporting a number of samples.
  • substrate plate 114 may be a 24, 36, 48, 72, 96 or 384 well plate.
  • apparatus 100 comprises one or more sample arrays 112, wherein the number of sample wells 116 in apparatus 100 is at least 100, preferably at least 1000, and more preferably at least 10,000.
  • the size of sample well 116 is about 1 mm to about 50 mm, more preferably about 2 mm to about 10 mm, and most preferably about 3 mm to about 7 mm.
  • a 3 mm well format provides an array of approximately 30,000 samples for 0.25 m 2 of skin.
  • sample array e.g. array 112
  • sample array e.g. array 112
  • each of the samples comprises at least two components, and at least one of the components being an active component.
  • one of the sample components is a "component-in-common”, which as used herein, means a component that is present in every sample of the array, with the exception of negative controls.
  • Sample array 112 is designed to provide a number of different samples of different compositions, the analysis of which allows determination of optimal compositions or formulations for improving transfer of a component across tissue 120.
  • Each sample in sample array 112 preferably, though not necessarily, differs from any other sample in the array with respect to at least one of:
  • a Ann a; rray can comprise 24, 36, 48, 96, or more samples, preferably at least 1000 samples, more preferably, at least 10,000 samples.
  • An array is typically comprised of one or more sub-arrays.
  • a sub-array can be a plate having 96 sample wells.
  • Overlaying substrate plate 114 and sample array 112 is tissue specimen 120.
  • Tissue 120 is preferably a sheet of tissue, such as skin, lung, tracheal, nasal, placental, vaginal, rectal, colon, gut, stomach, bladder, or comeal tissue. More preferably, tissue 120 is skin tissue or stratum comeum. If human cadaver skin is to be used for tissue 120, one known method of preparing the tissue specimen entails heat stripping by keeping it in water at 60°C for two minutes followed by the removal of the epidermis, and storage at 4°C in a humidified chamber. A piece of epidermis is taken out from the chamber prior to the experiments and placed over substrate plate 114.
  • Tissue 120 is optionally be supported by Nylon mesh (Terko Inc.) to avoid any damage and to mimic the fact that the skin in vivo is supported by mechanically strong dermis.
  • Nylon mesh Teko Inc.
  • other types of tissues maybe used, including living tissue explants, animal tissue (e.g. rodent, bovine or swine) or engineered tissue-equivalents.
  • animal tissue e.g. rodent, bovine or swine
  • engineered tissue-equivalents e.g., DERMAGRAFT (Advanced Tissue Sciences, Inc.) and those taught in U.S. Patent No.5,266,480, which is incorporated herein by reference.
  • tissue specimen 120 is divided into a number of segments by cuts 122 between sample wells 116 to prevent lateral diffusion through tissue specimen 120 between adjacent samples.
  • Cuts 122 may be made in any number of ways, including mechanical scribing or cutting, laser cutting, or crimping (e.g., between plates 114 and 130 or by using a "waffle iron" type embossing tool).
  • laser scribing is used as it avoids mechanical pressure from a cutting tool which can cause distortion and damage to tissue specimen 120.
  • Laser cuts 122 are performed with very small kerfs which permit a relatively high density of samples and a more efficient tissue specimen utilization. Laser tools are available that produce a minimal heat affected zone, thereby reducing damage to tissue specimen 120.
  • Reservoir plate 130 (e.g., an open-bottomed titer plate) is placed on top of tissue 120, on a side of tissue opposite substrate plate 114. Reservoir plate includes a number of hollow reservoirs 132. When plate 130 is secured in place, each reservoir 132 aligns over a sample well 116 such that tissue separates each well 116 from reservoir 132. Reservoir plate 130 secures to substrate plate 114 using clamps, screws, fasteners, or any other suitable attachment means. Plates 130 and 114 preferably secure together with sufficient pressure so as to create a liquid tight seal around reservoirs 132. Each reservoir is filled with a reservoir, such as a saline solution, to receive sample components or compounds that diffuse across tissue 120 to reservoir 132. In one embodiment, the reservoir medium is approximately 2% BSA solution in PBS.
  • Transfer or flux of components from sample wells 116 across tissue 120 may be analyzed by measuring component concentration in specimens taken from reservoirs 132. Comparison of measurements taken from different samples/reservoirs aids in determining optimal sample compositions for improving tissue transfer or diffusion of a desired component (e.g,. a pharmaceutical).
  • a desired component e.g,. a pharmaceutical
  • Preferably the samples are prepared, added to sample wells and mixed automatically. Similarly, specimen from reservoirs 132 containing transferred or diffused components, and the concentrations thereof, can be measured and processed automatically.
  • “Automated” or “automatically” refers to the use of computer software and robotics to add, mix and analyze the samples, components, and specimens or diffusion products.
  • Samples are added to the sample wells in sample arrays of the present invention, such as sample array 112 in FIG. 1, using various deposition or material transfer techniques known to the skilled artisan, including, without limitation, hand placement, pipetting, and other manual or automated solid or liquid distribution systems.
  • the samples may be processed by well known techniques, such as heating, filtration, and lyophilization.
  • heating, filtration, and lyophilization One of skill in the art will know how to process the sample according to the properties being tested.
  • the samples can be processed individually or as a group, preferably, as a group. Additional details regarding suitable automated dispensing and sampling equipment and methods of formulating solutions or compositions are disclosed in copending U.S. Patent Application Serial No.
  • Luminex Corp. Austin, TX, Beckman Instruments, Fullerton, CA, MicroFab Technologies, Piano, TX, Nanogen, San Diego, CA, and Hyseq, Sunnyvale, CA.
  • These devices test samples based on a variety of different systems. All include thousands of microscopic channels that direct components into test wells, where reactions can occur. These systems are connected to computers for analysis of the data using appropriate software and data sets.
  • the Beckman Instruments system can deliver nanoliter samples of 96 or 384-arrays, and is particularly well suited for hybridization analysis of nucleotide molecule sequences.
  • the MicroFab Technologies system delivers sample using inkjet printers to aliquot discrete samples into wells.
  • the combinations of active component and various additional or inactive components at various concentrations and combinations can be generated using standard formulating software (e.g., Matlab software, commercially available from Mathworks, Natick, Massachusetts).
  • the combinations thus generated can be downloaded into a spread sheet, such as Microsoft EXCEL.
  • a work list can be generated for instracting the automated distribution mechanism to prepare an array of samples according to the various combinations generated by the formulating software.
  • the work list can be generated using standard programming methods according to the automated distribution mechanism that is being used. The use of so-called work lists simply allows a file to be used as the process command rather than discrete programmed steps.
  • the work list combines the formulation output of the formulating program with the appropriate commands in a file format directly readable by the automatic distribution mechanism.
  • the automated distribution mechanism delivers at least one active component, such as a pharmaceutical, as well as various inactive or additional components, such as solvents, carriers, excipients, and additives, to each sample well.
  • the automated distribution mechanism can deliver multiple amounts of each component.
  • the automated distribution mechanism utilizes one or more micro-solenoid valves.
  • Automated liquid and solid distribution systems are well known and commercially available, such as the Tecan Genesis, from Tecan-US, RTP, North Carolina.
  • the robotic arm can collect and dispense active components and inactive components, such as solutions, solvents, carriers, excipients, additives, and the like, from a stock plate to a sample well or site. The process is repeated until an array is completed. The samples are then mixed. For example, the robotic arm moves up and down in each well plate for a set number of times to ensure proper mixing.
  • apparatus 100 of FIG. 1 is described above as having reservoir medium above tissue 120 in reservoirs 132 and samples below tissue 120 in sample wells 116 of array 112.
  • the positions are reversed, such that reservoirs 132 of sample array 112 are below tissue specimen 120 and sample wells 116 are above tissue specimen 120, and a top plate or top membrane is situated over reservoirs 132 and reservoir plate 130.
  • the term “component” means any substance or compound.
  • a component can be active or inactive.
  • the term “active component” means a substance or compound that imparts a primary utility to a composition or formulation when the composition or formulation is used for its intended purpose. Examples of active components include pharmaceuticals, dietary supplements, alternative medicines, and nutraceuticals. Active components can optionally be sensory compounds, agrochemicals, the active component of a consumer product formulation, or the active component of an industrial product formulation.
  • an “inactive component” means a component that is useful or potentially useful to serve in a composition or formulation for administration of an active component, but does not significantly share in the active properties of the active component or give rise to the primary utility for the composition or formulation.
  • the samples of an array comprise an active component and inactive components.
  • the active components in the samples of an array can be the same or different, while in another embodiment, the samples in an array comprise an active component as a component-in-common and inactive components.
  • sample means a mixture of an active component and one or more additional components or inactive components.
  • a sample comprises 2 or more additional components, more preferably, 3 or more additional components.
  • a sample will comprise one active component but can comprise multiple active components.
  • samples in a sample array may have one or more components-in-common.
  • a sample can be present in any container or holder or in or on any material or surface, the only requirement is that the samples be located at separate sites.
  • samples are contained in sample wells, for example, a 24, 36, 48, or 96 well plates (or filter plates) of volume 250 ⁇ l available from Millipore, Bedford, MA.
  • the sample can comprise less than about 100 milligrams of the active component, preferably, less than about 1 milligram, more preferably, less than about 100 micrograms, and even more preferably, less than 100 nanograms.
  • the sample has a total volume of about 1-200 ⁇ l, more preferably about 5-150 ⁇ l, and most preferably about 10-100 ⁇ l.
  • Samples can be liquid source or solid source samples, which include samples in the form of solids, semi-solids, films, liquids, solutions, gels, foams, pastes, ointments, triturates, suspensions, or emulsions.
  • the "physical state" of a component is initially defined by whether the component is a liquid or a solid. If a component is a solid, the physical state is further defined by the particle size and whether the component is crystalline or amorphous.
  • the physical state is further divided into: (1) whether the crystal matrix includes a co-adduct or whether the crystal matrix originally included a co-adduct, but the co-adduct was removed leaving behind a vacancy; (2) crystal habit; (3) morphology, i.e., crystal habit and size distribution; and (4) internal stracture (polymorphism).
  • the crystal matrix can include either a stoichiometric or non-stoichiometric amount of the adduct, for example, a crystallization solvent or water, i.e., a solvate or a hydrate.
  • Non-stoichiometric solvates and hydrates include inclusions or clathrates, that is, where a solvent or water is trapped at random intervals within the crystal matrix, for example, in channels.
  • a stoichiometric solvate or hydrate is where a crystal matrix includes a solvent or water at specific sites in a specific ratio. That is, the solvent or water molecule is part of the crystal matrix in a defined arrangement.
  • the physical state of a crystal matrix can change by removing a co-adduct, originally present in the crystal matrix. For example, if a solvent or water is removed from a solvate or a hydrate, a hole will be formed within the crystal matrix, thereby forming a new physical state.
  • the crystal habit is the description of the outer appearance of an individual crystal, for example, a crystal may have a cubic, tetragonal, orthorhombic, monoclinic, triclinic, rhomboidal, or hexagonal shape.
  • the processing characteristics are affected by crystal habit.
  • the internal stracture of a crystal refers to the crystalline form or polymorphism.
  • a given compound may exist as different polymorphs, that is, distinct crystalline species.
  • different polymorphs of a given compound are as different in stracture and properties as the crystals of two different compounds. Solubility, melting point, density, hardness, crystal shape, optical and electrical properties, vapor pressure, and stability, etc. all vary with the polymorphic form.
  • the component-in-common can be either an active component, such as a pharmaceutical, dietary supplement, alternative medicine, or nutraceutical, or an inactive component.
  • the component-in-common is an active component, and more preferably a pharmaceutical.
  • pharmaceutical means any substance or compound that has a therapeutic, disease preventive, diagnostic, or prophylactic effect when administered to an animal or a human.
  • pharmaceutical includes prescription drags and over the counter drugs.
  • Pharmaceuticals suitable for use in the invention include all those known or to be developed.
  • cardiovascular pharmaceuticals such as amlodipine besylate, losartan potassium, irbesartan, diltiazem hydrochloride, clopidogrel bisulfate, digoxin, abciximab, furosemide, amiodarone hydrochloride, beraprost, tocopheryl nicotinate; anti-infective components, such as amoxicillin, clavulanate potassium, azithromycin, itraconazole, acyclovir, fluconazole, terbinafine hydrochloride, erythromycin ethylsuccinate, and acetyl sulfisoxazole; psychotherapeutic components, such as sertraline hydrochloride, venlafaxine, bupropion hydrochloride, olanzapine, buspirone hydrochloride, alprazolam, methylphenidate hydrochloride, fluvoxamine
  • cardiovascular pharmaceuticals such as amlodipine besylate,
  • TAXOL paclitaxel Poor absorption due to its low water solubility.
  • NORVIR ritonavir Can undergo a polymorphic shift during shipping and storage.
  • FULVICIN griseofulvin Poor absorption due to its low water solubility.
  • Suitable veterinary pharmaceuticals include, but are not limited to, vaccines, antibiotics, growth enhancing components, and dewormers.
  • Other examples of suitable veterinary pharmaceuticals are listed in The Merck Veterinary Manual, 8th ed., Merck and Co., Inc., Rahway, NJ, 1998; (1997); The Encyclopedia of Chemical Technology, 24 Kirk-Othomer (4 th ed. at 826); and Veterinary Drugs in ECT 2nd ed., Vol 21, by A.L. Shore and R.J. Magee, American Cyanamid Co.
  • tissue (or trans-membrane) transfer analysis using the systems and methods of the present invention include dietary supplements, alternative medicines, or nutraceuticals.
  • dietary supplement means a non-caloric or insignificant-caloric substance administered to an animal or a human to provide a nutritional benefit or a non-caloric or insignificant-caloric substance administered in a food to impart the food with an aesthetic, textural, stabilizing, or nutritional benefit.
  • Dietary supplements include, but are not limited to, fat binders, such as caducean; fish oils; plant extracts, such as garlic and pepper extracts; vitamins and minerals; food additives, such as preservatives, acidulents, anticaking components, antifoaming components, antioxidants, bulking components, coloring components, curing components, dietary fibers, emulsifiers, enzymes, firming components, humectants, leavening components, lubricants, non-nutritive sweeteners, food-grade solvents, thickeners; fat substitutes, and flavor enhancers; and dietary aids, such as appetite suppressants.
  • suitable dietary supplements are listed in (1994) The Encyclopedia of Chemical Technology, 11 Kirk-Othomer (4 lh ed.
  • alternative medicine means a substance, preferably a natural substance, such as a herb or an herb extract or concentrate, administered to a subject or a patient for the treatment of disease or for general health or well being, wherein the substance does not require approval by the FDA.
  • suitable alternative medicines include, but are not limited to, ginkgo biloba, ginseng root, valerian root, oak bark, kava kava, echinacea, harpagophyti radix, others are listed in The Complete German Commission E Monographs: Therapeutic Guide to Herbal Medicine, Mark Blumenthal et al.
  • nutraceutical means a food or food product having both caloric value and pharmaceutical or therapeutic properties.
  • nutraceuticals include garlic, pepper, brans and fibers, and health drinks. Examples of suitable Nutraceuticals are listed in M.C. Linder, ed. Nutritional Biochemistry and Metabolism with Clinical Applications, Elsevier, New York, 1985; Pszczola et al, 1998 Food technology 52:30-37 and Shukla et ⁇ /., 1992 Cereal Foods World 37:665-666.
  • the active component when the active component is a pharmaceutical, a dietary supplement, an alternative medicine, or a nutraceutical, at least one additional component(s) is an excipient.
  • excipient means the inactive substances used to formulate pharmaceuticals as a result of processing or manufacture or used by those of skill in the art to formulate pharmaceuticals, dietary supplements, alternative medicines, and nutraceuticals for administration to animals or humans.
  • excipients are approved for or considered to be safe for human and animal administration.
  • excipients include, but are not limited to, acidulents, such as lactic acid, hydrochloric acid, and tartaric acid; solubilizing components, such as non-ionic, cationic, and anionic surfactants; absorbents, such as bentonite, cellulose, and kaolin; alkalizing components, such as diethanolamine, potassium citrate, and sodium bicarbonate; anticaking components, such as calcium phosphate tribasic, magnesium trisilicate, and talc; antimicrobial components, such as benzoic acid, sorbic acid, benzyl alcohol, benzethonium chloride, bronopol, alkyl parabens, cetrimide, phenol, phenylmercuric acetate, thimerosol, and phenoxyethanol; antioxidants, such as ascorbic acid, alpha tocopherol, propyl gallate, and sodium metabisulfite; binders, such as acacia, alginic acid, carboxymethyl cellulose
  • Excipients include those that alter the rate of absorption, bioavailability, or other pharmacokinetic properties of pharmaceuticals, dietary supplements, alternative medicines, or nutraceuticals.
  • suitable excipients such as binders and fillers are listed in Remington's Pharmaceutical Sciences, 18th Edition, ed. Alfonso Gennaro, Mack Publishing Co. Easton, PA, 1995 and Handbook of Pharmaceutical Excipients, 3rd Edition, ed. Arthur H. Kibbe, American Pharmaceutical Association, Washington D.C. 2000, both of which are incorporated herein by reference.
  • Excipients that are typically used in the formation of transdermal delivery devices, and therefore particularly useful for formulation of the samples of the present invention are penetration enhancers, adhesives and solvents. Each of these is discussed in more detail below.
  • Penetration Enhancers Various types may be used to enhance transdermal transport of drags. Penetration enhancers can be divided into chemical enhancers and mechanical enhancers, each of which is described in more detail below.
  • Chemical enhancers enhance molecular transport rates across tissues or membranes by a variety of mechanisms.
  • chemical enhancers are preferably used to decrease the barrier properties of the stratum comeum. Drag interactions include modifying the drug into a more permeable state (a prodrug), which would then be metabolized inside the body back to its original form (6-fluorouracil, hydrocortisone) (Hadgraft, 1985); or increasing drug solubilities (ethanol, propylene glycol).
  • cationic, anionic, and nonionic surfactants sodium dodecyl sulfate, polyoxamers
  • fatty acids and alcohols ethanol, oleic acid, lauric acid, liposomes
  • anticholinergic agents benzilonium bromide, oxyphenonium bromide
  • alkanones n-heptane
  • amides urea, N,N-diethyl-m- toluamide
  • fatty acid esters n-butyrate
  • organic acids citric acid
  • polyols ethylene glycol, glycerol
  • sulfoxides dimethylsulfoxide
  • terpenes cyclohexene
  • lipid permeation enhancers include interactions with the skin include enhancer partitioning into the stratum comeum, causing disruption of the lipid bilayers (azone, ethanol, lauric acid), binding and disruption of the proteins within the stratum comeum (sodium dodecyl sulfate, dimethyl sulfoxide), or hydration of the lipid bilayers (urea, benzilonium bromide).
  • Other chemical enhancers work to increase the transdermal delivery of a drug by increasing the drug solubility in its vehicle (hereinafter termed “solubility enhancers”).
  • solubility enhancers Lipid permeation enhancers, solubility enhancers, and combinations of enhancers (also termed “binary systems”) are discussed in more detail below.
  • Lipid Permeation Enhancers Chemicals which enhance permeability through lipids are known and commercially available. For example, ethanol increases the solubility of drugs up to 10, 000- fold and yield a 140-fold flux increase of estradiol, while unsaturated fatty acids increase the fluidity of lipid bilayers (Bronaugh and Maibach, editors (Marcel Dekker 1989) pp. 1-12. Examples of fatty acids which disrupt lipid bilayer include linoleic acid, capric acid, lauric acid, and neodecanoic acid, which can be in a solvent such as ethanol or propylene glycol.
  • Oleic acid was found to disorder the highly ordered SC lipid bilayers, and to possibly form a separate, oil-like phase in the intercellular domain.
  • SC Lipid bilayers disordered by unsaturated fatty acids or other bilayer disrupters may be similar in nature to fluid phase lipid bilayers.
  • a separated oil phase should have properties similar to a bulk oil phase. Much is known about transport a fluid bilayers and bulk oil phases. Specifically, diffusion coefficients in fluid phase, for example, dimyristoylphosphatidylcholine (DMPC) bilayers Clegg and Vaz In "Progress in Protein-Lipid Interactions" Watts, ed.
  • DMPC dimyristoylphosphatidylcholine
  • the diffusion coefficient of a given solute will be greater in a fluid bilayer, such as DMPC, or a bulk oil phase than in the SC. Due to the strong size dependence of SC transport, diffusion in SC lipids is considerably slower for larger compounds, while transport in fluid DMPC bilayers and bulk oil phases is only moderately lower for larger compounds. The difference between the diffusion coefficient in the SC and those in fluid DMPC bilayers or bulk oil phases will be greater for larger solutes, and less for smaller compounds. Therefore, the enhancement ability of a bilayer disordering compound which can transform the SC lipids bilayers into a fluid bilayer phase or add a separate bulk oil phase should exhibit a size dependence, with smaller permeability enhancements for small compounds and larger enhancement for larger compounds. [0108] A comprehensive list of lipid bilayer disrupting agents is described in
  • R-X wherein R is a straight-chain alkyl of about 7 to 16 carbon atoms, a nonterminal alkenyl of about 7 to 22 carbon atoms, or/& branched-chain alkyl of from about 13 to 22 carbon atoms, and X is -OH, -COOCH 3 , -COOC,H 5 , -OCOCH 3 , -SOCH 3 , -P(CH 3 ) 2 O, COOC 2 H 4 OC 4 H 4 OH, -COOCH(CHOH) 4 CH 3 OH, -COOCH 2 CHOHCH 3 , COOCH 2 CH(OR")CH 2 OR", -(OCH 2 CH 2 ) m OH, -COOR*, or -CONR' 2 , where R' is H, -CR 3 , -C 2 H 5 , -C,H 7 or -C 2 H 4 OH; R" is -H, or a non-terminal alkyl of from
  • Another way to increase the transdermal delivery of a drag is to use chemical solubility enhancers that increase the drug solubility in its vehicle. This can be achieved either through changing drag- vehicle interaction by introducing different excipients, or through changing drug crystallinity (Flynn and Weiner, 1993).
  • Solubility enhancers include water diols, such as propylene glycol and glycerol; mono-alcohols, such as ethanol, propanol, and higher alcohols; DMSO; dimethylformamide; N,N-dimethylacetamide; 2-pyrrolidone; N-(2-hydroxyethyl) pyrrolidone, N-methylpyrrolidone, l-dodecylazacycloheptan-2-one and other n-substituted-alkyl- azacycloalkyl-2-ones .
  • water diols such as propylene glycol and glycerol
  • mono-alcohols such as ethanol, propanol, and higher alcohols
  • DMSO dimethylformamide
  • 2-pyrrolidone N-(2-hydroxyethyl) pyrrolidone
  • N-methylpyrrolidone N-methylpyrrolidone
  • a binary system for enhancing metaclopramide penetration is disclosed in UK Patent Application GB 2,153,223 A, consisting of a monovalent alcohol ester of a C8-32 aliphatic monocarboxylic acid (unsaturated and/or branched if C 18-32) or a C6-24 aliphatic monoalcohol (unsaturated and/or branched if C14- 24) and an N-cyclic compound such as 2-pyrrolidone or N-methylpyrrolidone.
  • Combinations of enhancers consisting of diethylene glycol monoethyl or monomethyl ether with propylene glycol monolaurate and methyl Iaurate are disclosed in U.S. Patent No.
  • a penetration-enhancing vehicle containing specified amounts of one or more cell-envelope disordering compounds such as oleic acid, oleyl alcohol, and glycerol esters of oleic acid; a C 2 or C 3 alkanol and an inert diluent such as water.
  • cell-envelope disordering compounds such as oleic acid, oleyl alcohol, and glycerol esters of oleic acid
  • a C 2 or C 3 alkanol such as water.
  • Other chemical enhancers not necessarily associated with binary systems, include dimethylsulfoxide (DMSO) or aqueous solutions of DMSO such as those described in U.S. Patent No. 3,551,554 to Herschler; US. Patent No. 3,711,602 to Herschler and U.S. Patent No. 3,711,606 to Herschler, and the azones (n-substimted-alkyl-azacycloalkyl-2-ones) such as noted in U
  • the present invention enables testing of the effects of a large number of enhancers on tissue barrier transport, such as transdermal transport, of a compound, pharmaceutical, or other component.
  • tissue barrier transport such as transdermal transport
  • mechanical enhancers are defined as including almost any extraneous enhancer, such as ultrasound, mechanical or osmotic pressure, electric fields (electroporation or iontophoresis) or magnetic fields.
  • Pressure gradients can also be used to enhance movement of fluids across the skin. Pressure can be applied by a vacuum or a positive pressure device. Alternatively, osmotic pressure may be used to drive transdermal transport.
  • Application of magnetic fields to the skin pretreated or in combination with other permeation enhancers can be used to transport magnetically active species across the skin.
  • polymer microspheres loaded with magnetic particles could be transported across the skin.
  • Some devices for delivery of an active component or drug across a tissue barrier typically include an adhesive.
  • the adhesive often forms the matrix in which the active component or drag is dissolved or dispersed and, of course, is meant to keep the device in intimate contact with the tissue, such as skin.
  • Compatibility of the active component or drag with an adhesive is influenced by its solubility in that adhesive. Any supersaturated conditions produced in storage or in use are generally very stable against precipitation of the active component or drug within the adhesive matrix. A high solubility is desired in the adhesive to increase the driving force for permeation through the tissue and to improve the stability of the device.
  • Several classes of adhesive are used, each of which contain many possible forms of adhesives.
  • Acrylic adhesives are available in many derivatized forms. Thus, it is often a very difficult problem to select which adhesive might be best to use with any particular drag and enhancer.
  • all ingredients to be in the device are dissolved in a solvent and cast or coated onto a plastic backing material. Evaporation of the solvent leaves a drug-containing adhesive film.
  • the present invention enables rapid and efficient testing of the effects of various types and amounts of adhesives in a sample composition or formulation.
  • Solvents for the active component, carrier, or adhesive are selected based on biocompatibility as well as the solubility of the material to be dissolved, and where appropriate, interaction with the active component or agent to be delivered. For example, the ease with which the active component or agent is dissolved in the solvent and the lack of detrimental effects of the solvent on the active component or agent to be delivered are factors to consider in selecting the solvent.
  • Aqueous solvents can be used to make matrices formed of water soluble polymers.
  • Organic solvents will typically be used to dissolve hydrophobic and some hydrophilic polymers. Preferred organic solvents are volatile or have a relatively low boiling point or can be removed under vacuum and which are acceptable for administration to humans in trace amounts, such as methylene chloride.
  • solvents such as ethyl acetate, ethanol, methanol, dimethyl formamide (DMF), acetone, acetonitrile, tetrahydrofuran (THF), acetic acid, dimethyl sulfoxide (DMSO) and chloroform, and combinations thereof, also may be utilized.
  • Preferred solvents are those rated as class 3 residual solvents by the Food and Drag Administration, as published in the Federal Register vol. 62, number 85, pp. 24301-24309 (May 1997). Solvents for drugs will typically be distilled water, buffered saline, Lactated Ringer's or some other pharmaceutically acceptable carrier.
  • the high throughput screening methods of the present invention identify, for example, 1) optimal compositions or formulations comprising one or more active components and one or more inactive components for achieving desired characteristics for such compositions or formulations, 2) optimal adhesive/enhancer/excipient compositions for compatibility with an active component or drug, 2) optimal active component or drug/adhesive/enhancer/additive compositions for maximum drug flux through stratum comeum, and 3) optimal active component or drag/adhesive/enhancer/additive compositions to minimize cytotoxicity.
  • the basic requirements for sample preparation, processing, and screening are a distribution mechanism and a testing, or screening, mechanism.
  • the distribution mechanism adds components to separate sites on an array plate, such as into sample wells.
  • the distribution mechanism is automated and controlled by computer software and can vary at least one addition variable, e.g., the identity of the component(s) and or the component concentration, more preferably, two or more variables.
  • a sample such as a pharmaceutical component and excipients (e.g., enhancers and adhesives)
  • a sample such as a pharmaceutical component and excipients (e.g., enhancers and adhesives)
  • individual components can be placed into the appropriate well in the array manually.
  • a testing mechanism is preferably used to test each sample for one or more properties, such as drag concentration as a function of time.
  • the testing mechanism is automated and driven by a computer.
  • the system further comprises a processing mechanism to process the samples after component addition.
  • the samples can be processed by stirring, milling, filtering, centrifuging, emulsifying, or solvent removal (e.g., lyophilizing) and reconstituting, etc. by methods and devices well known in the art.
  • the samples are processed automatically and concurrently.
  • a preferred method of using the tissue barrier transfer device of FIG. 1 entails determining, directly or indirectly, the presence, absence or concentration of components (e.g. pharmaceuticals) that diffuse through tissue 120 into reservoir 132 of the array.
  • Such measurements may be performed by a variety of means known to those skilled in the art. For example, any know spectroscopic technique can be used to determine presence, absence or concentration of a component-in-common.
  • Suitable measurement techniques include, but are not limited to include spectroscopy, infrared spectroscopy, near infrared spectroscopy, Raman spectroscopy, NMR, X-ray diffraction, neutron diffraction, powder X-ray diffraction, radiolabeling, and radioactivity.
  • the passive permeabilities of active components e.g. a drag
  • radiolabelled compounds or drags are rotary evaporated in order to remove any solvent in which they are shipped and any tritium which had reverse exchanged into it.
  • the radiolabelled compounds or drags are then redissolved in various composition formulations, including enhancers, carriers, additives, adhesives, and/or other excipients as described infra, to a typical concentration of 1 ⁇ Chi/ml, and added to the sample wells, such as sample wells 116 of array 112 in FIG. 1. Passive permeation experiments are then performed.
  • PBS pH 7.4 phosphate buffer saline
  • phosphate concentration 0.01 M
  • NaCl concentration 0.137 M
  • Other receiver solutions may be used and are known to those skilled in the art.
  • the concentrations of radiolabelled component or drag in the sample and reservoir compartments are measured using a scintillation counter (e.g., model 2000 CA, Packard Instruments). Duplicate formulations may be used in some of the samples and/or repeated experiments may be performed to optimize reliability of measurements.
  • the passive permeability enhancements, E p is calculated relative to the passive permeability from PBS according to Eq. (1):
  • J"" (enhancer) and J * “ (PBS) are the drag fluxes from saturated solutions of enhancer and PBS, respectively.
  • the present invention includes a methods for repairing and/or correcting for microscopic defects on tissue specimens, such as skin.
  • tissue specimens such as skin.
  • apparatus or a diffusion cell used for study of transdermal delivery of active components require skin samples that are free of defect that might act as diffusional fast transport paths.
  • defects can be of several types with sizes ranging from millimeters to tens of microns.
  • Physical tears and hair follicles are just two types of defects that may compromise the interpretation of transport or diffusion data.
  • Inhomogeneous tissue segments, i.e. segments with an abnormal amount of defects will lead to inaccurate and misleading diffusion measurements, particularly when using relatively small tissue samples as in the present invention. Rapid identification of defect locations on the surface of a given tissue sample may be achieved by image analysis, preferably by high-speed micro inspection of each tissue segment using video microscopy or photomicrography.
  • diffusion data related to inhomogeneous tissue segments may be discarded to avoid inaccurate measurements.
  • associated diffusion measurements can be mathematically adjusted to account for the defects.
  • defects in a tissue specimen are repaired by feeding the defect locations to an ink jet printer that is instructed to print wax to cover these locations.
  • the print pattern is devised so as to cover the entire area of the defect with some possible overlap on to regions that are free of defects.
  • Wax print heads print molten wax that solidifies on impact with the tissue.
  • the solid wax is water-resistant and acts like a seal to ensure that the repaired region does not contribute to the diffusional flux during subsequent testing. Droplet placement preferably is such that overlap is sufficient to make a seal.
  • FIGS. 2A - 2D are schematic diagrams of an alternative high-throughput apparatus 200 and method for measuring tissue barrier transfer using a solid source sample.
  • Apparatus 200 is similar to apparatus 100 of FIG. 1, except that apparatus 200 is designed for testing solid source samples, such as compositions containing a semi-solid, such as an adhesive, a relatively flat transdermal patch, or a film-like sample.
  • Substrate plate 214 is a dense plate, such as a plastic or glass plate, that supports an array 212 of samples 216.
  • Each sample includes a combination of components, including an active component (e.g., a pharmaceutical) and at least one inactive component. Examples of suitable components are discussed above with respect to FIG. 1.
  • a first step of the method involves creating an array 212 of different composition regions (i.e., samples 216) on dense substrate 214.
  • the array may be produced in any number of ways, but one simple method is to use combinatorial dispensing equipment to make solutions of all the constituents in a convenient solvent. Suitable dispensing equipment and methods of formulating solutions or compositions are discussed above and disclosed in U.S. Patent Application Serial No. 09/540,462, which is herein incorporated by reference in its entirety.
  • the formulated solutions are contained in the wells of a microtiter plate similar to substrate plate 114 (of FIG. 1) that includes a sample array 112 of sample wells 116 and separable dense bottom plate 214 rather than base 118.
  • the solvent is then evaporated and each of the samples in the wells is allowed to dry to leave a film at the bottom. This evaporation process mimics the manufacturing process used to make various tissue transfer devices, such as transdermal patches.
  • the upper plate may then be removed to yield the array shown in FIG. 2A.
  • the samples 216 can be any shape, and preferably are generally round in shape as shown in FIG. 2A.
  • the plates of this format can be used to assess the stability of the compositions or formulations, such as drug/adhesive/enhancer solutions, toward precipitation of the active component, such as a pharmaceutical or drag.
  • Optical examination of each of the films will reveal if precipitation has occurred, since the precipitates may cause increased light scattering when the sample is illuminated.
  • Crystallization can also be optically detected by observing birefringence of crystals (for compounds that are birefringent).
  • Alternative means maybe used when the film is already sufficiently opaque to preclude the scattering method.
  • One such method is second harmonic generation (SHG) which easily detects the presence of crystals in the film.
  • SHG second harmonic generation
  • the next step of the present method is to prepare a tissue specimen 220 that is to be used in the study.
  • a specimen 220 such as a specimen of stratum comeum, may be conventionally prepared or obtained as described above. It is most convenient, however, that the sample specimen 220 should be sufficiently large to cover whatever plate format is used for the study. For example, it should be sufficiently large to cover a 96 well microtiter plate.
  • a separate tissue sample is prepared for each plate 214 of the study. The tissue is then placed on plate 214 so as to cover each of the sample regions, as shown in the FIG. 2B. Care is taken to insure that no air pockets are present under tissue 220.
  • One approach is to lay tissue 220 down on plate 214 starting at one edge and gently proceeding across the surface of the plate. The air is expelled ahead of the tissue/plate contact line.
  • the region of tissue 220 above each sample region may now be physically sectioned or isolated into segments 224 from neighboring regions to ensure that lateral diffusion does not occur between adjacent samples. As described above, this can be done in any number of ways, such as mechanical scribing or cutting, laser cutting or crimping along cuts 222.
  • Each of the tissue segments 224 on each plate 214 may now be imaged and characterized by video microscopy. Automated image recognition can be used to identify and record those tissue segments that are damaged or otherwise inhomogeneous. As described above, damaged or inhomogeneous tissue segments 224 may be replaced, repaired or ignored.
  • tissue 220 may be imaged and replaced or repaired prior to sectioning.
  • the tissue 220 is sectioned and/or imaged before placing tissue segments 224 over samples 216.
  • a next step in the present method is to place a reservoir plate 230, similar to reservoir plate 130 of FIG. 1 or an open-bottomed titer plate, over the tissue segments 224 as shown.
  • Reservoir plate 230 includes a number of hollow reservoirs 232. When plate 230 is secured in place, each reservoir 232 aligns over a sample and tissue such that a tissue segment 224 separates each sample from reservoir 232.
  • Reservoir plate 230 secures to substrate plate 214 using clamps, screws, fasteners, or any other suitable attachment means. Plates 230 and 214 preferably secure together with sufficient pressure so as to create a liquid tight seal around reservoirs 232.
  • Each reservoir is filled with a reservoir medium, preferably a liquid or solution, such as a saline solution, to receive sample compounds that diffuse across tissue segments 224 to reservoir 232.
  • the reservoir medium is approximately 2% BSA solution in PBS.
  • Incubation of the apparatus 200 with automated periodic sampling and makeup of the reservoir 232 solution is used to assess the permeability of the active component for all the samples of the combinatorial study.
  • the embodiments of the invention described herein are directed to movement of compounds across a tissue, the systems and methods of the present invention • are suitable for studying movement of compounds across any membrane or other barrier.
  • apparatus 300 relates to a method of high-throughput screening of active component flux through a tissue specimen, such as the stratum comeum, recognizing that such flux is determined, at least in part, by the permeability of the active component (such as a pharmaceutical or drug) within the tissue in the presence of an enhancer.
  • the permeability is generally governed by at least two factors: the solubility of the active component within the tissue (such as the stratum comeum) and the diffusivity of the active component within the tissue specimen. These two factors, solubility and diffusivity, are measured independently as a method of indirectly assessing the flux through the tissue specimen.
  • an array 312 of wells 316 containing samples (e.g. solutions 338) of different compositions of active components and inactive components (e.g., pharmaceutical/adhesive/enhancer/additive) is constructed.
  • samples e.g. solutions 338
  • inactive components e.g., pharmaceutical/adhesive/enhancer/additive
  • tissue segments 340 e.g. stratum comeum, are added to each well.
  • a tissue segment is placed on or over each well 316 (similar to the arrangement shown in FIGS. 1, 2C and 2D) such that each segment is in contact with a sample solution 338.
  • the rate at which a component (e.g., a drug, or pharmaceutical) is taken up into the tissue sample may be measured by extracting the tissue 340 from similarly prepared wells 316 at different times and measuring the presence, absence, or concentration of the component. Measuring the concentration after times sufficiently long so that the amount dissolved is not changing with time can assess solubility, or the equilibrium concentration of the component within the tissue 340. The product of the rate and solubility is proportional to the permeability of the component.
  • Diffusion cell 400 includes a sink plate 410, a source plate 430, and a tissue specimen 420 disposed between sink plate 410 and source plate 430.
  • Sink plate 410 includes a sink well 412 for holding reservoir medium as described above with respect to FIG. 1.
  • Sink well 410 is shown as having a cylindrical shape with an open end, however it may be rectangular, hexagonal, spherical, elliptical, or any other shape.
  • Sink plate 410 includes at least one access port 416 along an edge of sink well 412 that fluidly communicates with sink well 412.
  • Sink plate 410 also preferably includes a surface feature 414 configured to mate with source plate 430 and form a tight seal with tissue specimen 420.
  • tissue specimen 420 is skin tissue, but may be any tissue or membrane as described above with respect to tissue specimen 120 of FIG. 1.
  • Tissue specimen 420 is cut, formed or otherwise dimensioned to cover sink well 412 and surface feature 414. Tissue specimen 420 is placed such that it preferably does not completely cover access port 416.
  • source plate 430 includes a source reservoir, or well 432 that has open ends and aligns with sink well 412 when source plate 430 is placed on tissue specimen 420.
  • a passage 436 also passes through source plate 430 and is approximately adjacent to, but not in communication with, source well 432. Passage 436 is configured to align with access port 416 to provide access to the reservoir medium in sink well 412 without removing source plate 430.
  • source plate 430 also preferably includes a surface feature 434 that is configured and dimensioned to mate with surface feature 414 of sink plate 410 and form a seal with tissue specimen 420 around the perimeter of sink well 412 and source well 432.
  • surface feature 414 is a convex ring extending from the surface sink plate 420 around the open perimeter of sink well 412; and surface feature 434 is a concave ring formed in source plate 430 configured to mate with surface feature 414.
  • a number diffusion cells 400 are attached or formed together to create an array of diffusion cells similar to array 112 of FIG. 1.
  • Exemplary uses of the apparatus of FIGS. 4A - 4C are the same as those described above with respect to FIG. 1, except that access port 416 and passage 436 allow addition or removal of reservoir medium from sink well 412 without removing source plate 430 or tissue 420.
  • the reservoir medium used in diffusion cell 400 is a liquid or solution.
  • the placement of reservoir medium and sample could be reversed as in FIG. 1; for example, reservoir medium could be placed above tissue specimen 420 in source well 432 and sample could be held in sink well 412. In such an embodiment, sample may be added or removed through passage 436 and access port 416.
  • FIGS. 5 A and 5B show a schematic drawing of an apparatus 500 for use in adding or filling a sample 530 into a sample well 522 in a sample array, such as sample array 112 shown in FIG. 1, wherein the occurrence of air pockets or bubbles between the sample 530 and a tissue 524 is avoided.
  • the tissue 524 is located between a sample well 522, which is located in a substrate plate, such as substrate plate 114 shown in FIG. 1, and a reservoir 526, which is located in a reservoir plate, such as reservoir plate 130 shown in FIG. 1.
  • a feed canula 510 punctures a base membrane 520 which covers one side a the sample well 522 to be filled with sample 530.
  • sample feed source 514 is extended into sample well 522 until it is in contact with tissue 524.
  • Sample 530 is then fed through sample feed source 512, and as sample 530 begins to fill sample well 522, air is forced out of sample well 522 through air evacuation space 512 in feed canula 510.
  • sample feed source 512 and feed canula 510 are completely withdrawn from base membrane 520 and sample well 522.
  • sample feed source 514 retracts at a rate that is synchronized with the fill rate for sample 530 into sample well 522 such that at all times during the filling process, the outlet of sample feed source 514 is inside extruded sample 530 in sample well 522.
  • both sample feed source 512 and feed canula 510 are completely withdrawn from base membrane 520 and sample well 522.
  • base membrane 520 is a rubber membrane.
  • the filling method of the present invention can be performed by hand or using automated dispensing means, wherein sample wells in a sample array are filled using automated dispensing equipment that is capable of dispensing the same or different samples to multiple sample wells in one or more sample arrays in a fast, accurate, and controlled approach.
  • Sample 530 dispensed in accordance with the filling method of the present invention is preferably a liquid source sample.
  • FIG. 6 shows an exploded view, schematic diagram of a preferred embodiment of a high-throughput apparatus 600 for measuring tissue barrier transport in an array of solid source samples 630 according to the present invention.
  • Apparatus 600 comprises a base plate 610 supporting a spacer plate 620, an array of solid source samples 630, a tissue specimen 640, a reservoir plate 650 having an array of donor reservoirs 654, and a clamping means, such as shoulder screws 660 with threads 662.
  • base plate 610 is made aluminum, and spacer plate 620 and reservoir plate 650 are made of clear plastic or polycarbonate.
  • Base plate 610 has screw holes 612 which are drilled to mate with threads 662 on shoulder screws 660, such that when screws 660 are fed threw the apparatus into screw holes 612 and tightened, the apparatus is clamped together. When the apparatus is clamped together, a seal is formed between reservoir plate 650 and tissue specimen 640.
  • base plate 610 further comprises an array of guide marks 614, which can be any array formation, such as 2x2, 4x4, 6x6, and 8x12, which are used to help align various components of apparatus 600 during assembly.
  • screw holes 622 and screw holes 652 located around the edges of spacer plate 620 and reservoir plate 650, respectively, but preferably, the number of screw holes 622 and screw holes 652 is at least 4, and more preferably between 4 and 8. In a preferred embodiment, there is at least a screw hole at each comer of both spacer plate 620 and reservoir plate 650.
  • apparatus 600 further comprises a top plate located above reservoir plate 650, which is made out of the same material as base plate 610
  • Apparatus 600 is assembled by first placing spacer plate 620 on top of base plate 610 and aligning screw holes 622 in spacer plate 620 with screw holes 612 in base plate
  • An array of solid source samples 630 is created on spacer plate 620 in a pattern corresponding to the pattern of donor reservoirs 654 in reservoir plate 650, and guide marks
  • base plate 610 are used to ensure that each sample 630 is placed such that it aligns with a donor reservoir 654 in top plate 650.
  • the size of samples 630 are commensurate with the size of donor reservoirs 654.
  • Each sample 630 includes a combination of components, including an active component (e.g., a pharmaceutical) and at least one inactive component. Examples of suitable components are discussed above with respect to FIG. 1.
  • an active component e.g., a pharmaceutical
  • inactive component examples of suitable components are discussed above with respect to FIG. 1.
  • a sheet of tissue specimen 640 is placed over the array of samples 630 in a manner which avoids formation of air pockets between tissue specimen 640 and samples 630.
  • reservoir plate 650 having an array of donor reservoirs 654 is placed over the skin such that screw holes 652 on top plate 650 align with the corresponding screw holes 622 of spacer plate 620.
  • the resulting assembled apparatus 600 is then clamped together by sliding shoulder screws 660 with threads 622 through aligned screw holes 652 of assembled apparatus 600, and each shoulder screw 660 is tightened so as to form a seal between reservoir plate 650 and tissue specimen 640.
  • a shoulder screw 660 should be used in at least each of the four comers of the assembled apparatus 600.
  • a reservoir medium is added to donor reservoirs 654 of assembled apparatus
  • FIG. 7 shows a compressed view, schematic diagram of a high-throughput apparams 700 for measuring tissue barrier transport in an array of solid source samples according to the present invention.
  • Apparams 700 is the similar to apparatus 600, except the array of donor reservoirs 754 is an 8x12 array for a total of 96, wherein each reservoir is no more than 6mm in diameter.
  • Apparatus 700 comprises a base plate 710 supporting a spacer plate 720, an array of solid source samples (such as samples 630 shown in FIG. 6), a tissue specimen (such as tissue specimen 640 shown in FIG. 6), a reservoir plate 750 having an array of donor reservoirs 754, and a clamping means, such as shoulder screws 760.
  • FIG. 8 shows a compressed view, schematic diagram of a high-throughput apparatus 800 for measuring tissue barrier transport in an array of solid source samples according to the present invention.
  • Apparams 800 is the similar to apparatus 600 and apparams 700, except the array of donor reservoirs 854 is a 16x24 array for a total of 384 donor reservoirs 854 wherein each reservoir is no more than 3 mm in diameter.
  • Apparatus 800 comprises a base plate 810 supporting a spacer plate 820, an array of solid source samples (such as samples 630 shown in FIG. 6), a tissue specimen (such as tissue specimen 640 shown in FIG. 6), a reservoir plate 850 having an array of donor reservoirs 854, and a clamping means, such as shoulder screws 860.
  • FIG. 9 is an exploded view of a another transdermal assay apparatus according to another embodiment of the invention.
  • the transdermal assay apparatus 900 is similar to the apparatuses 600, 700, and 800 described above, as the apparatus 900 is also used for measuring the transport of a sample through a tissue barrier or specimen.
  • the sample is preferably a solid source sample, but alternatively may be any other suitable sample.
  • Transdermal assay apparatus 900 comprises first and second members 902 and
  • the first and second members are preferably made from stainless steel, coated appropriately to obtain the desired chemical resistance. These members could also be made from a clear plastic, such as polycarbonate, other plastics, or glass.
  • the first member 902 has opposing sides 905 and 922. These opposing sides are preferably substantially planar surfaces that are substantially parallel to one another.
  • One side 905 includes one or more sample surfaces 906 configured to receive one or more samples 908 thereon.
  • the sample surfaces 906 are preferably circular and arranged in an array, as shown.
  • the second member 904 also has opposing sides 910 and 914 that are also preferably substantially planar surfaces that are substantially parallel to one another.
  • the second member 904 defines one or more reservoirs 912 therein. The size of each reservoir 912 is similar to that described above in relation to FIGS. 7-8.
  • Each reservoir has an opening 934 on the side 910 of the second member 904. In a preferred embodiment, each opening 934 is substantially the same size as each sample surface 906.
  • each sample surface 906 is configured to substantially align with a corresponding opening 934 in a direction substantially perpendicular to the sample surface 906. Accordingly, in use, each sample 908 of an array of samples is configured to substantially align with a corresponding opening 934 of an array of openings.
  • the reservoirs 912 extend entirely through the second member 904 from the surface 910 to the opposing side 914, in a similar manner to that described above.
  • the transdermal assay apparatus 900 also preferably comprises a third member
  • the third member 916 is preferably made from aluminum, but could also be made from a number of other materials, such as steels, brass, plastics, ceramics or the like, that can be manufactured into a part that provides the desired geometry and dimensional stability.
  • the third member 916 has opposing sides 918 and 920 that are also preferably substantially planar surfaces that are substantially parallel to one another.
  • the third member 916 preferably includes a cavity 924 therein for receiving a magnet 926.
  • the magnet 926 is permanently formed in the third member 916, bonded to the third member, or the third member 916 itself is the magnet 926, i.e., the third member is made from a magnetic material.
  • the magnet 926 may be a permanent magnet, electromagnet, or the like. [0177]
  • the magnet 926 forms one portion of a magnetic clamp used to clamp first
  • the other portion of the magnetic clamp preferably comprises one or more inserts 928 embedded in the second member 904.
  • these inserts 928 are made from a ferrous material that is attracted to the magnet 926.
  • the inserts 928 are magnetic inserts that are attracted to the magnet 926, i.e., the magnetic inserts and the magnet 926 are arranged to have opposite polarities facing one another.
  • These magnetic inserts are preferably permanent magnets, but alternatively may be electromagnets.
  • the first and third members are made from a ferrous or magnetic material (such as 430 stainless steel). These magnetic members are then attracted to hold by the magnet in the third member to provide the desired clamping force.
  • the third member also preferably includes at least one, but preferably two or more, alignment posts 930 configured to mate with complementary alignment holes 932 in the first member 902.
  • the alignment posts are preferably made from a ferrous material to aid the attraction between the magnet 926 and the inserts 928, and to hold the magnet 926 in place in the cavity 924.
  • the magnetic clamp may take on various other forms.
  • the magnetic clamp may comprise one or more magnets and/or ferrous inserts disposed in, on, or near the first member 902, second member 904, and/or third member 916.
  • these members may be fully or partially made from ferrous and/or magnetic materials.
  • the strength of the magnet 926, or the gap between the magnet(s) and insert(s) or magnetic material(s) is preferably chosen to provide the required clamping force between the members.
  • the magnet 926 can be replaced by a stronger or weaker magnet to alter the resulting clamping force and to optimize the sealing among the members of the apparatus 900.
  • the magnet 926 is bonded to the third member
  • a sample 908 is arranged on each sample surface 906 of the first member 902, such that each sample surface 906 will align with a corresponding opening 934.
  • the size of the samples 908 are commensurate with the size of each opening 934.
  • Each sample 908 includes a combination of components, including an active component (e.g., a pharmaceutical) and at least one inactive component. Examples of suitable components are discussed above with respect to FIG. 1.
  • the second member 902 is then placed onto the third member 916 such that the alignment posts 930 mate with the alignment holes 932. This aligns the second member 902 in a predetermined position on the third member 916.
  • a sheet of tissue specimen 936 is placed over the samples 908 in a manner which avoids formation of air pockets between the tissue specimen 936 and the samples 908.
  • the second member 904 is then placed over the tissue specimen 936, such that the tissue specimen 936 is exposed to each reservoir's opening 934.
  • This magnetic attraction between the magnet 926 and inserts 928 also creates a clamping force that clamps the first, second, and third members together, thereby clamping the tissue specimen between each sample 908 and each opening 934.
  • FIG. 10 is an exploded view of a yet another transdermal assay apparatus 1000 according to yet another ernbodiment of the invention.
  • the transdermal assay apparatus 1000 is similar to the transdermal assay apparatus 900 (FIG. 9) described above.
  • Transdermal assay apparatus 1000 includes a sample plate and flexible magnet, as described below.
  • the sample is preferably a solid source sample, but alternatively may be any other suitable sample.
  • Transdermal assay apparatus 1000 comprises a first member 1010 and a second member 1002. These members are preferably made from stainless steel, coated appropriately to obtain the desired chemical resistance. These members could also be made from a clear plastic, such as polycarbonate, other plastics, or glass. These members also preferably, have opposing planar surfaces and are substantially parallel to one another.
  • the second member 1002 defines one or more reservoirs 1014 therein, each having an opening, as described above in relation to FIG. 9.
  • a flexible magnet 1008 is disposed between the first and second plates.
  • a sample array, preferably disposed on a sample plate 1006 is disposed between the flexible magnet 1008 and the second plate.
  • a tissue sample 1004 is disposed between the sample plate 1006 and the second plate 1002.
  • the sample plate 1006 includes one or more sample surfaces 1012 configured to receive one or more samples thereon.
  • the sample surfaces 1012 are preferably circular and arranged in an array, as shown.
  • each sample surface 1012 is configured to substantially align with a corresponding opening of each reservoir 1014 in a direction substantially perpendicular to the sample surface 1012.
  • each sample of an array of samples is configured to substantially align with a corresponding opening of an array of openings.
  • the reservoirs 1014 extend entirely through the second member 1002, in a similar manner to that described above.
  • the flexible magnet 1008 forms one portion of a magnetic clamp used to clamp the first 1010 and second 1002 members together.
  • the second plate 1002 is preferably made from a ferrous material that is attracted to the flexible magnet 1008.
  • ferrous inserts are placed in the second member 1002.
  • the transdermal assay apparatus 1000 is used as described above.
  • Example [0190] The following Example further illustrates the method and arrays of the present invention. It is to be understood that the present invention is not limited to the specific details of the Example provided below.
  • Example 1 Nicotine Permeation Across Human Cadaver Skin
  • Human cadaver skin epidermis was prepared by first separating skin from the underlying fat and then separating the epidermis by heat treatment at 60°C for 90 seconds using standard techniques.
  • a NICODERM CQ ® brand nicotine Step 1 (21 mg/24 hours) transdermal patch (sold by Glaxo SmithKline, Research Triangle Park, NC USA) was punched into 5/16" diameter circles, keeping the backing and release liners on the resulting punched samples until such were deposited in the test apparatus.
  • each plate in the apparams was a rectangular shape having dimensions of 5.030" (127.76 mm) by 3.365" (85.48 mm).
  • the apparatus was assembled by first placing a 1/8" (3.175 mm) thick clear polycarbonate spacer plate 620 on top of an aluminum base plate 610 and aligning screw holes 622 in the spacer plate with screw holes 612 in the base plate. Thereafter, a 4x4 sample array was created on spacer plate 620, as described in Table 2 below:
  • Punched samples were placed on spacer plate 620 in the 4x4 array of Table 2 one at a time, and the location of each array sample was selected using guide marks 614 on base plate 610 to ensure that each array sample was placed such that it aligned with a donor reservoir 654 in reservoir plate 650.
  • the release liner on each sample was removed to expose the drag reservoir/adhesive of the sample.
  • the resulting assembled apparatus 600 was clamped together by sliding a shoulder screw 660 with threads 622 through aligned screw holes 652 at each of the four comers of the assembled apparatus, and tightening each shoulder screw 660 so as to form a seal between reservoir plate 650 and the skin.
  • the screw holes 612 on base plate 610 had a 10-24 tap, ranging between 0.250" (6.35 mm) and 0.188 (4.775 mm), which gripped threads 662 of screws 660 as the screws were tightened, thereby clamping the apparatus together.
  • PBS Dulbecco's Phosphate Buffered Saline
  • each donor reservoir 654 in the array resulted in four (4) 50 ml test aliquots that were diluted as set forth above, except that the test aliquot taken at 3 hours for the B3 donor reservoir was diluted to 950 ml rather than 500 ml.
  • the nicotine content in each test aliquot was then determined by HPLC analysis .

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Abstract

L'invention concerne un appareil (600) de dosage transdermique comprenant un premier (610), un deuxième (650) et un troisième élément (640). Le premier élément (610) présente une ou plusieurs surfaces d'échantillon, étant chacune configurée pour recevoir un échantillon (630). Le deuxième élément (650) définit un ou plusieurs réservoirs (654), comprenant chacun une ouverture sur une surface du deuxième élément. Chaque surface d'échantillon est sensiblement de même dimension que chaque ouverture. L'appareil de dosage transdermique comprend également un dispositif de blocage magnétique configuré pour bloquer un spécimen de tissu entre la surface d'échantillon et l'ouverture. Le dispositif de blocage magnétique comprend, de préférence, un aimant avec une force sélectionnée en fonction de la force de blocage nécessaire entre le premier élément et le deuxième élément. L'invention concerne également un procédé d'utilisation d'un appareil de dosage transdermique.
EP03777948A 2002-10-28 2003-10-28 Dosage transdermique a l'aide d'un dispositif de blocage magnetique Withdrawn EP1556501A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/282,505 US6852526B2 (en) 2000-07-14 2002-10-28 Transdermal assay with magnetic clamp
US282505 2002-10-28
PCT/US2003/034262 WO2004040006A1 (fr) 2002-10-28 2003-10-28 Dosage transdermique a l'aide d'un dispositif de blocage magnetique

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EP1556501A1 true EP1556501A1 (fr) 2005-07-27
EP1556501A4 EP1556501A4 (fr) 2009-04-08

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DE102005057031B4 (de) * 2005-11-25 2011-04-21 Innovent E.V. Technologieentwicklung Vorrichtung für Permeations-oder Stoffdurchgangsuntersuchungen
EP2205967B1 (fr) * 2007-10-17 2013-05-01 Syneron Medical Ltd. Vérification de la vitesse de dissolution
JP2011133417A (ja) * 2009-12-25 2011-07-07 Lvmc Inc 活性酸素種濃度測定用センサーを用いた皮膚化粧料用抗酸化物質の簡便なスクリーニング方法
JP6418711B2 (ja) * 2014-05-19 2018-12-05 株式会社イントロテック 透過性試験装置
FR3100334B1 (fr) * 2019-09-03 2021-07-30 Fabre Pierre Dermo Cosmetique Dispositif pour la détermination automatisée du passage percutané et de la métabolisation d’une substance

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WO2004040006A1 (fr) 2004-05-13
AU2003286736A1 (en) 2004-05-25
EP1556501A4 (fr) 2009-04-08

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