EP1507919B1 - Behandlung von tierischen haarfasern mit modifizierten proteasen - Google Patents

Behandlung von tierischen haarfasern mit modifizierten proteasen Download PDF

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Publication number
EP1507919B1
EP1507919B1 EP02736309A EP02736309A EP1507919B1 EP 1507919 B1 EP1507919 B1 EP 1507919B1 EP 02736309 A EP02736309 A EP 02736309A EP 02736309 A EP02736309 A EP 02736309A EP 1507919 B1 EP1507919 B1 EP 1507919B1
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EP
European Patent Office
Prior art keywords
treatment
proteases
wool
fibre
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP02736309A
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English (en)
French (fr)
Other versions
EP1507919A1 (de
Inventor
Artur Cavaco Paulo
Carla Joana Dos Santos Marinho Da Silva
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidade do Minho
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Universidade do Minho
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Application filed by Universidade do Minho filed Critical Universidade do Minho
Publication of EP1507919A1 publication Critical patent/EP1507919A1/de
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Classifications

    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
    • D06M13/10—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing oxygen
    • D06M13/12—Aldehydes; Ketones
    • D06M13/123—Polyaldehydes; Polyketones
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
    • D06M2101/02—Natural fibres, other than mineral fibres
    • D06M2101/10—Animal fibres
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
    • D06M2101/02—Natural fibres, other than mineral fibres
    • D06M2101/10—Animal fibres
    • D06M2101/12—Keratin fibres or silk
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M2200/00—Functionality of the treatment composition and/or properties imparted to the textile material
    • D06M2200/45—Shrinking resistance, anti-felting properties

Definitions

  • the cuticle layer of animal hair fibres presents a scaly structure when observed by microscopy.
  • the felting or shrinkage of these fabrics is due to the overlapping of these scales that surround the cortex (inner part of the fibre), in wet processes with high mechanical agitation.
  • the removal of the cuticle layer makes it possible to eliminate the tendency of the protein fibres of animal origin to shrink.
  • One possibility of anti-felt treatment would be the application of proteolytic treatments for the removal of the cuticle layer. This kind of treatment has been extensively studied since the beginning of the 20 th century, but without great achievements.
  • Plasma treatment is a dry process, which involves treating wool fibre material with electric gas discharges (so-called plasma).
  • plasma electric gas discharges
  • the patent JP 6341067 describes the use of water-soluble immobilized proteases for the treatment of several textile materials, where the enzyme can be recovered and reused. This patent however does not describe the use of activating agents and therefore free enzymes could be also present in solution.
  • the patent JP-A 51099196 describes a process to treat wool fabrics with alkaline proteases.
  • the patent JP-A 3213574 describes a method for the treatment of wool with transglutaminase or a solution having this enzyme.
  • the patents US 6051033 describe a method of wool or wool fibres treatment with a proteolytic enzyme and transglutaminase.
  • WO 98/27264 describes a method to reduce the shrinking of wool that consists of bringing the fibre samples into contact with a solution of peroxidase or oxidase under the adequate conditions for the enzymatic reaction with wool.
  • the patent US 6099588 relates a method to improve shrink resistance that may result in improvements in feel, appearance and felting, among others, by the application of proteolytic enzymes in an aqueous solution, after treatment with an alkaline solution containing alcohol.
  • the patent US 5.529.928 refers to a process for obtaining wool with anti-felt finishing, a soft feel and with shrink resistance using an initial chemical oxidation followed by a treatment with protease and warming.
  • the patent EP 134267 uses a similar process, treating the fibre with proteolytic enzymes in the presence of salt, after the initial oxidative treatment.
  • the patent EP 3.58386 describes a method of wool treatment that consists of a proteolytic treatment and one of, or both, an oxidative treatment (such as NaOCl) and treatment with polymer.
  • This invention relates to a new anti-felting method of treating animal hair fibres characterized by the contact of the animal hair fibres with a solution of proteases that were chemically modified by glutaraldehyde and/or ⁇ -aminopropyltriethoxysilane to increase its molecular weight in order to reduce their diffusion inside the fibre.
  • the cuticle will be the only accessible part to the proteolytic attack, which allows for the improvement of one or more wool properties, including their felting and shrinking, without damaging the fibre's interior.
  • the methodologies used to increase the molecular weight of the enzymes are based on the utilization of a soluble polymer with hydroxyl groups activated with ⁇ -aminopropyltriethoxysilane and/or glutaraldehyde.
  • the glutaraldehyde may subsequently bind to another polymer chain, forming a polymeric net, or to an available protein NH 2 group.
  • the method consists in the treatment of the protein material with a solution of modified proteolytic enzymes.
  • Commercially available proteases from Sigma (Subtilisin kind) were used.
  • the immobilisation was performed on a soluble polymer, polyvinyl alcohol (Sigma), of average molecular weight 70000-100000, using glutaraldehyde (Aldrich), ⁇ -aminopropiltrietoxisylane, and/or Borax (Sigma) and polyethylenglycol (Sigma) of 10000 of average molecular weight.
  • the polymer at 6% (w/v) solution in distilled water was dissolved with warming and stirring, activated, and then was added to a 2% (v/v) glutaraldehyde solution. This solution was kept under stirring at room temperature, for 2 hours. After this time, the solution was dialysed in 0.1 M pH 5.0 acetate buffer for 24 hours and then in 0.05 M pH 3.95 acetate buffer for 20 hours.
  • the enzymatic preparation in the desired concentration was added to the resulting solution, together with PEG (1.25%) and Borax (0.05 ⁇ g/mL) in 0.1 M pH 5.0 acetate buffer, and kept under stirring for 8 hours at room temperature. This solution was kept at 4°C until use. The immobilisation procedure did not cause any significant loss in activity.
  • Samples of pure merino wool fabric (like animal hair fibre) with about 12 cm x 12 cm (of about 3 grams, each), were placed in a recipient containing a solution of proteases being chemically modified or not, in a relation of 1/20 (w/v).
  • the treatment was performed at 37°C, for periods of time ranging from 4 to 48 hours.
  • the samples were removed from the solution, washed and air-dried. They were then subjected to tests to evaluate possible damage caused during the treatment.
  • the tendency of the fabrics to shrink was verified by washing the fabrics (11 x 6 cm) three times in distilled water containing 50 ⁇ L of a wetting agent for 60 minutes, at 50°C and 20 rpm, and the shrinkage was measured by the variation of the specimen dimensions. It was verified that only the enzymatically treated fabrics did not induce a significant shrinkage.
  • the tendency to shrink was verified by washing wool yarns three times in distilled water having 50 ⁇ L of a wetting agent for 60 minutes, at 50°C and 20 rpm, and shrinkage was quantified by the visual verification of yarn felting. It was verified that only the enzymatically treated yarns did not induce felting.

Landscapes

  • Engineering & Computer Science (AREA)
  • Textile Engineering (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
  • Cosmetics (AREA)

Claims (7)

  1. Verfahren zur Antifilzausrüstung von Tierfasem, dadurch gekennzeichnet daß man die Tierfasem mit einer Proteasenlösung, die durch Glutaraldehyd und/oder γ-Aminopropyltriethoxysilan chemisch modifiziert worden ist, in Berührung bringt, um das Molekular-Gewicht der Fasern zu steigern, sodaß die Diffusion derselben im Innern der Fasern vermindert wird.
  2. Verfahren nach Anspruch 1 bei dem das Aktivationsmittel Glutaraldehyd ist.
  3. Verfahren nach Anspruch 1 wobei die modifizierten Proteasen an lösbaren Polymer gebunden sind.
  4. Verfahren nach Anspruch 1 wobei die lösbaren Polymer in wässrigen Lösungen wie z.B. Polyvinylalkohol und/oder Polymer wie z.B. Hydroxylgruppen verwendet werden, als Träger bei der chemischen Veränderung von Proteasen.
  5. Verfahren nach Anspruch 1 wobei das betreffende proteolitische Enzym ein Proteasenenzym ist.
  6. Verfahren nach Anspruch 5 wobei die Serinprotease Subtilisin ist.
  7. Verfahren nach Anspruch 1 wobei die Menge von Protease pro Kilo Wolle, Faser oder Haar zwischen 1 bis 1000g steht.
EP02736309A 2002-05-21 2002-05-21 Behandlung von tierischen haarfasern mit modifizierten proteasen Expired - Lifetime EP1507919B1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/PT2002/000008 WO2003097927A1 (en) 2002-05-21 2002-05-21 Treatment of animal hair fibers with modified proteases

Publications (2)

Publication Number Publication Date
EP1507919A1 EP1507919A1 (de) 2005-02-23
EP1507919B1 true EP1507919B1 (de) 2008-03-26

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EP02736309A Expired - Lifetime EP1507919B1 (de) 2002-05-21 2002-05-21 Behandlung von tierischen haarfasern mit modifizierten proteasen

Country Status (6)

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US (1) US20060121595A1 (de)
EP (1) EP1507919B1 (de)
AT (1) ATE390506T1 (de)
AU (1) AU2002309359B2 (de)
DE (1) DE60225850T2 (de)
WO (1) WO2003097927A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI119700B (fi) * 2002-12-16 2009-02-13 Suedwolle Gmbh & Co Kg Villan teollinen biotekninen viimeistysmenetelmä ja menetelmällä valmistettu villatekstiili
PT104124B (pt) * 2008-07-04 2011-10-14 Univ Do Minho Enzima proteolítica geneticamente modificada para o tratamento de fibras de animais de forma a aumentar a resistência das mesmas ao encolhimento, respectivo processo de obtenção e aplicação
CN102243169B (zh) * 2011-04-25 2012-11-14 上海嘉麟杰纺织品股份有限公司 一种检测羊毛损伤程度的方法
CN102978956A (zh) * 2012-10-31 2013-03-20 江南大学 一种羊毛织物复合酶浸轧-室温打卷堆置防毡缩整理工艺
CN103046385B (zh) * 2012-12-24 2014-08-27 江阴兴吴呢绒科技有限公司 一种粗纺可机洗纯羊毛面料的制造工艺
US9222216B2 (en) 2014-04-09 2015-12-29 University Of Calcutta Methods for enzymatic treatment of wool
CN108149417B (zh) * 2018-02-23 2020-06-09 上海嘉芮实业有限公司 一种羊毛针织成衣的缩绒方法及包括该方法的羊毛强缩绒针织物的制作工艺及其制品

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH538003A (fr) * 1968-03-29 1973-01-31 Anvar Procédé pour l'obtention d'articles textiles porteurs d'enzymes
JPH06341067A (ja) * 1993-04-12 1994-12-13 Osaka Prefecture 繊維構造物の加工処理剤及び加工処理方法
TR199501643A2 (tr) * 1994-12-21 1996-07-21 Nova Nordisk As Yünün enzimlerle tedavisi icin yöntem.
TR200003419T2 (tr) * 1998-05-20 2001-03-21 Novo Nordisk Biochem North America, Inc. Yünün enzimatik işleme tabi tutulması için yöntem
US6051033A (en) * 1998-05-20 2000-04-18 Novo Nordisk Brochem North America Inc. Method for enzymatic treatment of wool
US6099588A (en) * 1999-02-23 2000-08-08 Novo Nordisk Biochem North America, Inc. Method for treatment of wool

Also Published As

Publication number Publication date
DE60225850D1 (de) 2008-05-08
ATE390506T1 (de) 2008-04-15
AU2002309359B2 (en) 2009-01-08
US20060121595A1 (en) 2006-06-08
DE60225850T2 (de) 2009-02-05
AU2002309359A1 (en) 2003-12-02
EP1507919A1 (de) 2005-02-23
WO2003097927A1 (en) 2003-11-27

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