EP1330649A1 - Surfaces a separateur hydrophile lie de maniere covalente a des hydrogels - Google Patents
Surfaces a separateur hydrophile lie de maniere covalente a des hydrogelsInfo
- Publication number
- EP1330649A1 EP1330649A1 EP00975986A EP00975986A EP1330649A1 EP 1330649 A1 EP1330649 A1 EP 1330649A1 EP 00975986 A EP00975986 A EP 00975986A EP 00975986 A EP00975986 A EP 00975986A EP 1330649 A1 EP1330649 A1 EP 1330649A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- hydrogel
- residue
- derivatives
- organic molecules
- carboxylic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/808—Optical sensing apparatus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
Definitions
- the hydrophilic polymer layer on the surface has the character of a hydrogel, so it is swellable and also flexible. Both properties are desirable for the function of this layer, since receptor molecules are fixed on and in it, which must be linked so flexibly that they are still able to bind analyte molecules after immobilization.
- the previously known hydrogels on biosensor surfaces therefore have the schematic structure shown in FIG. 1.
- the surface of the sensor comprises a metal layer 14, to which one or more intermediate layers 13 may be applied.
- the receptor 11 is bound to the hydrogel 12 via a short, inflexible spacer.
- the binding of receptor molecules to the hydrogel layer can either be directional or non-directional.
- the receptor molecule usually has only one residue or possibly only a few (for example less than three) residues of the same type which can be reacted with the optionally derivatized hydrogel.
- the resulting directional bond is therefore spatially precisely defined.
- the receptor molecules have to be derivatized before implementation so that they have a suitable reactive residue.
- the receptor molecule has a large number (for example at least three) of the same type of residues, which can be reacted with the possibly devated hydrogel. Since only one of these residues reacts with the hydrogel, the position of the bond cannot be predicted spatially.
- the binding site or the binding sites of the receptor is in an undefined steric arrangement to the hydrogel, which in turn impairs the accessibility of these binding sites for analyte molecules and can accordingly influence the binding kinetics of the analyte / receptor interactions.
- US Pat. No. 5,395,587 uses short- and long-chain spacers to bind biotin as a receptor molecule. However, no influence on the length of the spacer is described. In addition, only the directional connection of receptor molecules and not the non-directional connection is examined.
- the aim of the invention was to provide an object, preferably a biosensor, to which a large number of receptor molecules can be attached. Pre-treatment of the receptor molecules should preferably not be necessary.
- the length of the spacer between the hydrogel and the receptor plays a role in the binding behavior of analyte molecules in the case of undirected bonds in objects with a hydrogel layer.
- This opens up the possibility of providing objects which enable a better binding behavior to certain problem situations.
- Improved accessibility due to increased flexibility makes the binding behavior of the analyte molecules more uniform despite the non-directional binding of the receptors and the behavior of the two free species in solution is more similar.
- the invention thus relates to an object with a surface, comprising a hydrogel, obtainable by:
- FIG. 2 shows a schematic illustration of an object according to the invention. It differs from the conventional object shown in FIG. 1 in that the receptor is bound to the hydrogel via a long-chain spacer.
- the invention further relates to a method for producing an object with a functionalized hydrogel surface, comprising the steps: (i) binding organic molecules to the hydrogel, the bound organic molecules each having a chain length of at least 8 atoms and a terminal residue A, selected from amine radical, carboxylic acid group or derivatives thereof; and (ii) reacting the terminal residue A with a receptor molecule with at least three identical residues B, selected from the amine residue, carboxylic acid group, sulfonic acid residue, phosphoric acid residue, phosphonic acid residue and their derivatives.
- the invention further describes the use of an object according to the invention in the detection of analyte molecules.
- an object with a functionalized hydrogel surface comprising organic molecules bound to the hydrogel, the bound organic molecules each having a chain length of at least 8 atoms and having a terminal radical A selected from the amine radical, carboxylic acid group or their derivatives undirected attachment of a receptor molecule with at least three identical residues B, selected from amine residue, carboxylic acid group, sulfonic acid residue, phosphoric acid residue, phosphonic acid residue and their derivatives.
- FIG. 1 shows a schematic representation of a conventional object with a hydrogel surface, in which the receptors are bound to the hydrogel by short spacers.
- FIG. 2 shows a schematic representation of an object according to the invention with a hydrogel surface, in which the receptors are bound to the hydrogel by flexible, long-chain spacers.
- FIG. 3 shows the time course of the covalent binding of lysozyme in the sensor (b) according to the invention and the comparison sensor (a) in example 1.
- FIG. 4 shows the time course of the association reaction of a single-chain fragment antibody to lysozyme in the sensor (b) according to the invention and the comparison sensor (a) of example 1.
- the effect in FIGS. 1 and 2 is removed by calculation due to the changed bulk refractive index when the analyte is added.
- the effects of changing refractive indices were measured and subtracted on inert, non-functionalized surfaces (i.e. hydrogel without functional groups and receptors).
- the objects of the invention are found, for. B. as sensors, especially biosensors, used in a wide variety of analytical measurement methods.
- suitable areas of use for the objects are in affinity-based sensors, such as surface plasmon resonance spectroscopy (SPR) and quartz scales, and in interferometric measurement methods, for example B. reflection interference contrast microscopy and reflection interference spectroscopy. They are particularly suitable for use in the SPR.
- SPR surface plasmon resonance spectroscopy
- interferometric measurement methods for example B. reflection interference contrast microscopy and reflection interference spectroscopy. They are particularly suitable for use in the SPR.
- the structure of the non-functionalized surface depends on the analytical method in which the object according to the invention is to be used and is known to the person skilled in the art (Journal of Biomedical Materials Research, 18 (953-959) (1984) and J. Chem. So ⁇ , Chem. Commun., 1990, 1526).
- non-functionalized surface is used to denote the surface of an object with the hydrogel layer before the organic molecules are attached to the residue A.
- functionalized surface is used to refer to the surface of an object with the hydrogel layer after the organic molecules have bound to the residue A.
- the object of the invention has a base surface, comprising, for. B. a glass, semiconductor or metal layer. Metal layers are preferred, especially noble metal layers such. B. of gold or silver such as. B. find use in the SPR.
- the non-functionalized object has a hydrogel layer on the surface. This layer serves to prevent unspecific adsorption, which falsifies the measurement signal.
- Hydrogels are water-swellable polymers.
- the hydrogels can be e.g. B. a polysaccharide, a derivative thereof or a swellable organic polymer such as poly ⁇ N- [tris (hydroxymethyl) methyl] acrylic acid amide ⁇ , polyvinyl alcohol or polyethylene glycol.
- Polysaccharides are preferred.
- Amino derivatives or carboxyalkyl derivatives can be mentioned as derivatives, the alkyl radical preferably having 1 to 4 carbon atoms. Examples of polysaccharides are amylose, inulin, pullulan or dextran. Pullulan or dextran and their derivatives are preferred. Dextran and its derivatives are particularly preferred. Carboxymethyldextran is preferably used.
- the hydrogel layer should be several nanometers thick when dry and swells to a thickness of approx. 100 nm in an aqueous environment, which completely covers the surface.
- the swollen polymer layer mimics the natural environment of biomolecules and is suitable for preventing denaturation and thus inactivation of the biomolecules.
- the adsorption of molecules other than the molecules to be analyzed is effectively suppressed.
- the swollen hydrogel layer is able to compensate for surface irregularities. Biomolecules are also bound in the swollen matrix and not just on the surface. This will reduce the importance of surface irregularities, which otherwise contribute to a poorly defined surface and thus to poorly quantifiable measurement results.
- Organic molecules which have a radical A are bound to this non-functionalized hydrogel layer.
- the bound organic molecules have a terminal radical A, selected from the amine radical, carboxylic acid group and their derivatives. Although primary amine residues are preferred, secondary amine residues can be combined with a can also be used.
- .carboxylic acid groups e.g. B. anhydrides and carboxylic acid halides, such as carboxylic acid chlorides, can be used.
- the terminal radical A is preferably a carboxylic acid group.
- the bound organic molecules each have a chain length of at least 8 atoms.
- the binding behavior of analyte molecules can be influenced over the length of the chain.
- the chain has 8 to 40 atoms, more preferably 10 to 20 atoms.
- the chain can be a substituted or unsubstituted, branched or unbranched hydrocarbon chain.
- the chain is preferably linear.
- these chains are preferably hydrophilic. Long alkyl chains or aromatic residues in the chain or as substituents thereon are less suitable, since these could also cause non-specific adsorption via hydrophobic interactions.
- the hydrophilic character of the spacers can be determined by the incorporation of oligoethylene oxide units (KL Prime & GM Whitesides, J. Am.
- amine residues, carboxylic acid groups and their derivatives can also be used as substituents.
- the type or number of the substituents used in the invention must not be selected such that they impair the flexibility of the organic molecules, which is important according to the invention.
- the chain length is the number of C, N, O and S atoms from the hydrogel in the main chain to the terminal residue, which is included. If part of the terminal residue is split off when the receptor molecule is bound, the split-off atoms are not counted. When counting, only the atoms of the organic molecules and the atoms, e.g. B. are due to the derivatization of the hydrogel counted.
- reaction conditions for coupling the organic molecules to the hydrogel layer vary depending on the compound selected. Examples of these reaction conditions are described below in the preferred embodiments and examples.
- the receptor molecule is used for the specific binding of the analyte molecule. That's why it's usually a biomolecule.
- suitable receptor molecules are proteins, nucleic acids and biologically active oligosaccharides or polysaccharides. Proteins are preferred.
- the invention relates to the non-directional connection of receptors to a hydrogel surface. Consequently, the receptor molecules have at least 3, preferably at least 5, more preferably at least 10 identical residues B. The maximum number of residues B is not limited, but the receptor molecule should be soluble in the solvent used.
- the receptor molecule preferably has at most 10,000, more preferably at most 1000 residues B
- the reactivity of the residues of the same type need not be identical, but is of the same order of magnitude.
- the residues B are selected from the amine residue, carboxylic acid group, sulfonic acid residue, phosphoric acid residue, phosphonic acid residue and their derivatives Do not hinder molecules. Suitable derivatives are, for example, ester derivatives of the acids mentioned with at least one -OH group, in order to enable reaction with the radical A.
- Analyte molecules react selectively with the receptors used. They are therefore usually also biomolecules. Suitable analyte molecules are proteins, nucleic acids and biologically active oligosaccharides or polysaccharides. Proteins are preferred.
- the conditions under which the analyte molecule is covalently bound to the receptor vary depending on the system selected. Typically, the binding is carried out at room temperature and in buffered, aqueous solutions which have an analyte concentration in the range from 0.5 to 200 ⁇ g / ml and about 100 mM buffer concentration, for example a phosphate buffer, typically the duration of the reaction is 10 minutes to 2 hours
- the precious metal surface e.g. gold
- a monolayer of cysteamine in which the precious metal surface is in an aqueous for 12 to 36 h
- the polyacrylic acid is typically reacted for 15 minutes to 2 hours with an aqueous solution of a hydrogel.
- the diagram shows carboxymethyl dextran as an example of the hydrogel.
- the organic molecules are built up in two reaction steps. First, the carboxy groups are reacted with 1,2-diaminoethane before the carboxymethyldextrans is attached to the surface. After the hydrogel has been bound to the surface, the terminal carboxylic acid end group is then introduced by reacting the amino group with bromoacetic acid. Usually a 0.5 mol • I "1 to 3 mol • I " 1 bromoacetic acid solution with a pH of about 14 is used for derivatization. Dehvatisation usually takes 10 to 20 hours.
- the organic molecules can link to the hydrogel in a single reaction step.
- This functionalization can take place either before or after the attachment of the hydrogel to the surface.
- the length of the spacer is 8 atoms.
- the C, N and O atoms of the spacer consisting of -CH 2 -CO-NH-CH 2 -CH 2 -NH-CH 2 -CO-OH are counted, the O of the -C (O) OH group not being counted , because in the subsequent implementation z. B. is replaced with an amine group of the analyte.
- Another preferred embodiment is:
- the reaction with succinic anhydride can take place in a 10 2 to 10 "" 'mol f 1 succinic anhydride solution.
- the reaction time is usually 4 up to 24 h.
- Z. B. polar, aprotic solvents such as dry DMSO.
- reaction with oligoethylene oxide takes place in an aqueous
- the reaction can take 10 min take up to 2 hours.
- the sensor according to the invention has the structure described in the first exemplary embodiment.
- a sensor with a short spacer is used as a comparative example:
- Lysozyme is used as the receptor in both sensors, and a single chain fragment of a corresponding antibody (HyHel 10 antibody) serves as the ligand.
- a glass support with a gold surface is placed in a water solution of 2 10 " 2 mol I " 1 cysteaminium hydrochloride solution for 12 h.
- the support is then rinsed with ultrapure water, incubated for 5 min with 1 N NaOH and rinsed again with ultrapure water.
- An incubation solution is obtained by mixing an aqueous 5 10 _2 mol r 1 polyacrylic acid solution (molecular weight 30,000) with solutions of 3, 19 mg EDC or 4.1 15 mg NHS, each made in 1 ml ultrapure water.
- the carrier is incubated for 1 h in this solution and then rinsed with ultrapure water
- the hydrogel dextran is first devatized according to the following method. 10.00 g (0.062 mol repetition unit). Dextran is dissolved in 50 ml of ultrapure water with 0.99 g NaOH (0.025 mol) and 1.71 g bromoacetic acid (0.012 mol) and added for 24 hours Stirred at room temperature Then the mixture is dialyzed against distilled water for 3 days, the water being changed at least five times. For further reaction, the dialyzed mixture is mixed with 2.40 g of ethyl (3-dimethylamino-propyl) carbodimide (0.0125 mol) and 1.44 g of N-hydroxysuccinimide are added and the mixture is stirred for 12 hours at room temperature.
- the mixture is then dialyzed again against distilled water for 3 days, the water being changed at least five times. 90% of the water is then removed using a rotary evaporator at reduced pressure and the polymer is introduced into the 10 times the volume of methanol.
- the yield is 9.06 g (81.5%)
- the aminodextran obtained is then dissolved in water, so that a 10% by weight solution results.
- the carrier is placed in this solution for 30 minutes and then rinsed again with plenty of ultrapure water.
- the carrier is then placed in a solution of 1 mol • 1 ⁇ 1 bromoacetic acid and 2 mol • 1 "1 NaOH for 12 h.
- the hydrogel is 10 min in 10 mM 4 '- (2-hydroxyethyl) -1 - piperazinethanesulfonic acid buffer (hepespuffer) and 150 mM NaCl at pH 7.4 in the presence of 77 mg-ml ' 1 EDC and 12 mg -ml '1 NHS enabled.
- a 0.1 mg-ml "1 solution of lysozyme in acetate buffer at pH 4.7 is then contacted with the hydrogel in order to covalently bind the lysozyme to the hydrogel by reaction between the free amino groups of the protein and activated carboxyl groups of the hydrogel.
- the functionalized sensor is installed in an SPR device.
- the SPR device used is a self-made with ⁇ / 2 ⁇ construction (analogous to E.
- FIG. 3 shows the time course of the covalent binding of lysozyme in comparative example (a) and in the sensor (b) according to the invention.
- the shift of the minimum to higher angles serves as an indicator of the increase in the layer thickness.
- an analogous behavior is observed when the analyte is bound.
- a single chain fragment of a lysozyme antibody is used.
- the association reaction is completed after about 15 minutes, while in the comparison sensor it takes about 60 minutes to reach saturation (see FIG. 4).
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- Health & Medical Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
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- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
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- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
L'invention concerne un objet ayant une surface comprenant un hydrogel, que l'on obtient : 1) par liaison de molécules organiques à l'hydrogel, les molécules organiques liées ayant chacune une longueur de chaîne d'au moins 8 atomes et un reste terminal choisi parmi reste amine, groupe acide carboxylique et leurs dérivés ; 2) puis par réaction des restes terminaux avec une molécule réceptrice munie d'au moins trois restes similaires choisis parmi reste amine, groupe acide carboxylique, reste acide sulfonique, reste acide phosphorique, reste acide phosphonique et leurs dérivés.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/EP2000/010810 WO2002037107A1 (fr) | 2000-11-02 | 2000-11-02 | Surfaces a separateur hydrophile lie de maniere covalente a des hydrogels |
Publications (1)
Publication Number | Publication Date |
---|---|
EP1330649A1 true EP1330649A1 (fr) | 2003-07-30 |
Family
ID=8164148
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP00975986A Withdrawn EP1330649A1 (fr) | 2000-11-02 | 2000-11-02 | Surfaces a separateur hydrophile lie de maniere covalente a des hydrogels |
Country Status (4)
Country | Link |
---|---|
US (1) | US7229840B1 (fr) |
EP (1) | EP1330649A1 (fr) |
AU (1) | AU2001213911A1 (fr) |
WO (1) | WO2002037107A1 (fr) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE366418T1 (de) | 1996-04-25 | 2007-07-15 | Bioarray Solutions Ltd | Licht-regulierte, elektrokinetische zusammensetzung von partikeln an oberflächen |
US9709559B2 (en) | 2000-06-21 | 2017-07-18 | Bioarray Solutions, Ltd. | Multianalyte molecular analysis using application-specific random particle arrays |
DE60117556T2 (de) | 2000-06-21 | 2006-11-02 | Bioarray Solutions Ltd. | Multianalytische molekularanalyse durch verwendung anwendungsspezifischer zufallspartikelarrays |
US7262063B2 (en) | 2001-06-21 | 2007-08-28 | Bio Array Solutions, Ltd. | Directed assembly of functional heterostructures |
US20040002073A1 (en) | 2001-10-15 | 2004-01-01 | Li Alice Xiang | Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection |
AU2003298655A1 (en) | 2002-11-15 | 2004-06-15 | Bioarray Solutions, Ltd. | Analysis, secure access to, and transmission of array images |
US7927796B2 (en) | 2003-09-18 | 2011-04-19 | Bioarray Solutions, Ltd. | Number coding for identification of subtypes of coded types of solid phase carriers |
PT1664722E (pt) | 2003-09-22 | 2011-12-28 | Bioarray Solutions Ltd | Polielectrólito imobilizado à superfície com grupos funcionais múltiplos capazes de se ligarem covalentemente às biomoléculas |
US7563569B2 (en) | 2003-10-28 | 2009-07-21 | Michael Seul | Optimization of gene expression analysis using immobilized capture probes |
NZ547494A (en) * | 2003-10-28 | 2008-05-30 | Bioarray Solutions Ltd | Gel-shell beads with absorbed or bound biomolecules |
ES2533876T3 (es) | 2003-10-29 | 2015-04-15 | Bioarray Solutions Ltd | Análisis multiplexado de ácidos nucleicos mediante fragmentación de ADN bicatenario |
US7848889B2 (en) | 2004-08-02 | 2010-12-07 | Bioarray Solutions, Ltd. | Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification |
US8486629B2 (en) | 2005-06-01 | 2013-07-16 | Bioarray Solutions, Ltd. | Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation |
US20070191848A1 (en) * | 2006-02-01 | 2007-08-16 | Zimmer Technology, Inc. | Hydrogel bone plate spacer |
JP5772612B2 (ja) * | 2011-01-27 | 2015-09-02 | コニカミノルタ株式会社 | 表面プラズモン励起増強蛍光分光法を利用する蛍光測定装置用センサチップを用いたアッセイ方法、およびアッセイ用キット |
EP2713877B1 (fr) | 2011-05-23 | 2019-07-31 | Roche Diabetes Care GmbH | Dispositif de détection pour détecter un analyte |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE462454B (sv) * | 1988-11-10 | 1990-06-25 | Pharmacia Ab | Maetyta foer anvaendning i biosensorer |
WO1992003732A2 (fr) | 1990-08-28 | 1992-03-05 | Bioprobe International, Inc. | Compositions et procedes pour obtenir une liaison amelioree dans des analyses biologiques |
DE4310141A1 (de) * | 1993-03-29 | 1994-10-06 | Boehringer Mannheim Gmbh | Homobidentale trifunktionelle Linker |
US5395587A (en) * | 1993-07-06 | 1995-03-07 | Smithkline Beecham Corporation | Surface plasmon resonance detector having collector for eluted ligate |
DE19817180C2 (de) * | 1998-04-17 | 2000-04-27 | Biotul Bio Instr Gmbh | Biosensor mit modifizierter Edelmetalloberfläche und Verfahren zu seiner Herstellung |
DE19916638C2 (de) | 1999-04-13 | 2002-11-07 | Jandratek Gmbh | Oberflächen zur Verbesserung des Bindungsverhaltens von Analytmolekülen auf der Basis kovalent gebundener hydrophiler Spacer an Hydrogelen |
-
2000
- 2000-11-02 WO PCT/EP2000/010810 patent/WO2002037107A1/fr not_active Application Discontinuation
- 2000-11-02 AU AU2001213911A patent/AU2001213911A1/en not_active Abandoned
- 2000-11-02 EP EP00975986A patent/EP1330649A1/fr not_active Withdrawn
- 2000-11-02 US US10/415,306 patent/US7229840B1/en not_active Expired - Fee Related
Non-Patent Citations (1)
Title |
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See references of WO0237107A1 * |
Also Published As
Publication number | Publication date |
---|---|
US7229840B1 (en) | 2007-06-12 |
AU2001213911A1 (en) | 2002-05-15 |
WO2002037107A1 (fr) | 2002-05-10 |
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