EP1317961A1 - Recipient pourvu d'un bouchon - Google Patents

Recipient pourvu d'un bouchon Download PDF

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Publication number
EP1317961A1
EP1317961A1 EP02024788A EP02024788A EP1317961A1 EP 1317961 A1 EP1317961 A1 EP 1317961A1 EP 02024788 A EP02024788 A EP 02024788A EP 02024788 A EP02024788 A EP 02024788A EP 1317961 A1 EP1317961 A1 EP 1317961A1
Authority
EP
European Patent Office
Prior art keywords
vessel
lidded
softer
lid
hollow cylinder
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP02024788A
Other languages
German (de)
English (en)
Other versions
EP1317961B1 (fr
Inventor
Jürgen Löhn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Publication of EP1317961A1 publication Critical patent/EP1317961A1/fr
Application granted granted Critical
Publication of EP1317961B1 publication Critical patent/EP1317961B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric

Definitions

  • the invention relates to a lidded container made of plastic for laboratory use, especially under elevated temperature conditions, i.a. in the PCR technique.
  • Lidded vessels of the type mentioned initially have a capacity of a few milliliters or below one milliliter.
  • Known lidded vessels comprise a vessel and a lid with a lid bottom and a hollow cylinder on one side of the lid bottom, either plugged into a vessel opening or cap-like on the The end of the vessel is placed with the vessel opening to the vessel outer wall seal.
  • the lid may be formed separately from or with the vessel be connected via a hinge.
  • the lid may leak or jump up, when the pressure in the vessel increases above ambient pressure. This can be, for example when tempering samples in the lidded vessels occur.
  • PCR polymerase chain Reaction
  • the PCR involves the three steps of denaturation at 94 ° C, the Annealing at 40 to 60 ° C and DNA synthesis at 72 ° C, which often (mostly 25 up to 30 times).
  • the object of the invention is to provide a lidded container create, in which the lid better against leaks and / or jumping up due to a pressure difference between the interior of the vessel and the Environment is protected.
  • the sealing partners i. of the Hollow cylinder of the lid and the vessel inner wall or the vessel outer wall, in Area of the sealing seat with a bias to each other, to a seal to achieve.
  • the hollow cylinder and the vessel have a flexural rigidity, which ensures a simple and secure closing of the lid, without this one preventing deformation of the sealing areas.
  • the circumferential sealing areas are also very stiff radially, so that at elevated Pressure in the vessel no or hardly enhances the sealing effect Expansion of the inner sealing partner is possible.
  • the axial section of the hollow cylinder is free End of the hollow cylinder or goes the axial portion of the vessel from the end of the Vessel with the vessel opening, resulting in a particularly strong expandability at the free end of the hollow cylinder or at the end of the vessel with the vessel opening is achieved with a concomitant increase in the sealing effect and the holding force.
  • the axial portion of the lid extends to the lid bottom or the axial portion of the vessel extends at least over the entire coverage area the hollow cylinder of the attached lid.
  • the harder and softer segments axially parallel or inclined or interlocking edges to the axis.
  • the axis-parallel or inclined to the axis edges have in particular spraying Advantages. With the axis inclined or interlocking edges a constructive clawing of the segments can be achieved, which in their Manufacture of different materials can be beneficial.
  • the hollow cylinder or the vessel at least in Region of the axial section inside and / or outside a soft material layer.
  • the hollow cylinder at the free end or the vessel at the vessel opening on a sealing bead can be the sealing effect in particular Increase by increasing the surface pressure.
  • the sealing bead is softer than the harder segments.
  • the coefficient of friction between the vessel and the Lid be increased to increase the holding power.
  • the lid or the vessel at the proximal end the axial section at least one completely or partially circumferential ring section which is softer than the harder segments.
  • the at least one Ring section can the radial expandability or compressibility of the Axial section increase, especially when moving along the base of the harder Segments extends.
  • the ring portion at the base of the hollow cylinder in Lid bottom arranged and softer than the rest of the lid bottom, also around the increase radial expandability or compressibility of the axial section.
  • Radial sections which extend radially from the ring portion to the outside and also softer than the rest of the lid bottom are provided.
  • the softer regions i.e. Segments and / or the sealing bead and / or the ring section and / or the radial sections
  • the adjacent harder areas i.e., the harder ones
  • the softer regions i.e. Segments and / or the sealing bead and / or the ring section and / or the radial sections
  • a softer material than the adjacent harder areas (i.e. harder segments and / or other areas of the lid or vessel).
  • the softness is increased by using softer materials. The softness can Combined with reduced wall thickness and material usage.
  • the lid and / or the vessel is within the softer ones Areas everywhere from the same softer material and outside of it Areas everywhere made of the same harder material.
  • the lid and / or the Vessel then consist of only two components.
  • the harder material is polypropylene or polyethylene and / or the softer material silicone or a thermoplastic elastomer or an elastomer or other soft polymeric material.
  • the harder material and the softer material plastics are used which have particularly good adhesive properties with each other.
  • the lidded vessel comprises at least two plastic components with different E-modules.
  • the softer ones Segments made of a material whose modulus of elasticity is one or more orders of magnitude is less than the modulus of elasticity of the material making up the harder segments consist.
  • the invention includes the formation of the vessel and lid each as a separate Component. Furthermore, the invention comprises vessels and lids which are separate and mutually connectable components are, for example by means of a tab that with a the components is integrally connected and a ring for connection to the outer periphery having the other component.
  • vessels and lids which are separate and mutually connectable components are, for example by means of a tab that with a the components is integrally connected and a ring for connection to the outer periphery having the other component.
  • the lid and the vessel are integrally in a one- or multi-component injection molding produced. You can then in particular, comprise a film hinge which connects the lid and vessel together. But also includes the possibility of lid and vessel over a Separating point releasably connect to each other, the separation point to close of the vessel is to be separated.
  • the lid is in its middle radius region made of an elastic plastic so as to be perforated, e.g. by means of a Needle for sampling purposes to ensure automatic sealing properties.
  • the lidded vessel according to FIGS. 1 to 3 has a vessel 1 with a cup-shaped one Floor 2, an adjacent cone section 3 and an adjacent thereto Cylinder section 4, a vessel opening 5 and a surrounding this Vessel flange 6 has.
  • the lidded vessel has a lid 7 with a lid bottom 8, which in the example has approximately the form of two identical, isosceles trapezoids, the are juxtaposed at the big baseline.
  • the vessel flange 6 is connected to the lid base 8 via two parallel hinge bands 9, which have adjacent to the vessel flange 6 side sections 10, in which they are practically not flexible.
  • the lid base 8 has at the edge of a skirt 14 which protrudes from its inside. In the region of the locking lug 13, the skirt 14 is interrupted.
  • the lid base 8 carries inside a hollow cylinder 15, the entire circumference has a distance from the enclosure 14 and protrudes beyond this.
  • the hollow cylinder 15 is alternately in the circumferential direction in harder segments 16th and softer segments 17, which extend axially over the entire Hollow cylinder 15 extend.
  • the harder segments 16 may be in their ratio be made variable to the softer segments 17 in larger areas and optimally have a ratio of 1: 1 in terms of their extent in Circumferential direction.
  • the lid bottom 8 has at the base of the hollow cylinder inside a on the inner circumference the hollow cylinder encircling ring portion 19, which also consists of a softer Material is as the segments 16.
  • the lid base 8 has on the side opposite the film hinges 9 in Distance range of the skirt 14 and the hollow cylinder 15 is a teardrop-shaped, softer area 20, which passes through the lid bottom 8.
  • the softer segments 17, the sealing bead 16, the ring portion 19 and the Drop-shaped portions 20 are made of the same softer material.
  • the remaining areas of the lid base 8 are made of the same harder material as the harder segments 16.
  • the hinges 9, the locking elements 12, 13 and the vessel 1 are also made of this material.
  • the entire lidded vessel is in the two-component injection molding of the two Materials made. After spraying the harder material are over the ring portion 19, the softer segments 17 in the gaps between the segments 16, the sealing bead 18 and the teardrop-shaped portion 20 with the softer Material filled up.
  • the lid 7 is by pressing the hollow cylinder 15 into the vessel opening. 5 closed.
  • the sealing bead 15 comes under bias to the plant at the Vessel inner wall, i. forms with this a sealing seat. In this sealing position supports the laterally over the hollow cylinder 15 projecting bottom 8 on top of the Vessel flange 6 from.
  • the hollow cylinder 15 expands due to the soft, stretchable segments 17 on.
  • the seal between the sealing bead 15 and vessel inner wall is thereby reinforced and also the of the Vessel inner wall on the sealing bead 15 exerted frictional force. This is the Cover 7 against pressing by acting on the lid bottom 8 force secured.
  • the harder material of the lid 7 'according to the invention is a PP having a modulus of elasticity of 1400 Newton / mm 2 and the conventional lid 7 "is made entirely of this material was based on a TPE with an E modulus of 6.1 Newton / mm 2 .
  • FIGS. 6 and 7 another lid 7 "'harder segments 16' '' and softer segments 17 '' 'with obliquely inclined to the axis sides, wherein the harder segments 16 '' 'to the lid bottom 8' 'and the softer segments 17' '' tapering towards the free end of the hollow cylinder 15 '' '.
  • the cover 7 IV of FIG. 8 and 9 differs from the lid 7 of FIG. 1 to 3 characterized in that at the base of the hollow cylinder 15 IV a ring portion 19 IV on the shell of the hollow cylinder 15 IV completely rotates and above the harder segments 16 IV to the top of the lid bottom 8 IV ranges.
  • the undercut for producing the ring portion 19 IV can be realized by a slotted, annular tool core, which dives through the lid 7 IV .
  • the rigidity of the cover bottom 8 V of the cover 7 V is reduced by ring sections 21 in the cover base 8 V and radial sections 22 originating therefrom and made of a softer component. This results in a reduction of the rigidity of the hollow cylinder 15 V and hereby likewise an improvement of the Aufsp Rudmony.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Closures For Containers (AREA)
  • Pressure Vessels And Lids Thereof (AREA)
  • Sealing Devices (AREA)
  • Table Devices Or Equipment (AREA)
  • Packages (AREA)
EP02024788A 2001-12-05 2002-11-07 Recipient pourvu d'un bouchon Expired - Lifetime EP1317961B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10159804A DE10159804B4 (de) 2001-12-05 2001-12-05 Deckelgefäß
DE10159804 2001-12-05

Publications (2)

Publication Number Publication Date
EP1317961A1 true EP1317961A1 (fr) 2003-06-11
EP1317961B1 EP1317961B1 (fr) 2004-10-20

Family

ID=7708170

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02024788A Expired - Lifetime EP1317961B1 (fr) 2001-12-05 2002-11-07 Recipient pourvu d'un bouchon

Country Status (5)

Country Link
US (1) US6783025B2 (fr)
EP (1) EP1317961B1 (fr)
AT (1) ATE279986T1 (fr)
DE (2) DE10159804B4 (fr)
ES (1) ES2227376T3 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2468404A3 (fr) * 2010-12-23 2013-01-23 Eppendorf AG Récipient à couvercle
US11040806B2 (en) 2017-12-15 2021-06-22 Husky Injection Molding Systems Ltd. Closure cap for a container
EP3978116A1 (fr) * 2020-10-01 2022-04-06 Eppendorf AG Récipient à couvercle en matière plastique et procédé de marquage d'un récipient à couvercle en matière plastique

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DE602004004955T2 (de) * 2003-08-20 2007-12-06 Sysmex Corp. Probenanalysegerät und eine Vorrichtung zur Detektion von Nukleinsäuren
US20060289552A1 (en) * 2005-06-27 2006-12-28 Danks Christopher A Closure with hinged lid
US8051998B1 (en) * 2005-06-28 2011-11-08 Csp Technologies, Inc. Product container with integral selective membrane
EP1741488A1 (fr) 2005-07-07 2007-01-10 Roche Diagnostics GmbH Recipient et procédé pour le traitement automatisé des liquides
EP1757367B1 (fr) * 2005-07-07 2010-03-31 Roche Diagnostics GmbH Récipient et procédé pour le traitement automatisé des liquides
FR2889461B1 (fr) * 2005-08-04 2007-10-26 Gilson Sas Soc Par Actions Sim Microtube de laboratoire.
DE102005058399A1 (de) * 2005-12-01 2007-06-14 Eppendorf Ag Deckelgefäß
KR100869636B1 (ko) 2006-10-20 2008-11-21 최영화 안전용기
US7749452B2 (en) * 2007-01-25 2010-07-06 Gemu Gmbh Sample and reaction container
WO2009001986A1 (fr) * 2007-06-28 2008-12-31 Choi, Young Hwa Récipient sûr
DE102008023522B3 (de) * 2008-05-15 2009-12-17 Inotech Kunststofftechnik Gmbh Vorrichtung und Verfahren zum Spenden von fließfähigen Substanzen sowie Verfahren zum Herstellen einer Vorrichtung zum Spenden von fließfähigen Substanzen
US20100281955A1 (en) * 2009-05-05 2010-11-11 Pressure Biosciences Inc. Microtube and related methods therefor
EP2485958A2 (fr) * 2009-10-09 2012-08-15 Qiagen Dispositif de fermeture et son procédé d'utilisation
US8397945B2 (en) * 2010-02-23 2013-03-19 R.J. Reynolds Tobacco Company Dispensing container
EP2633329B1 (fr) * 2010-10-29 2019-03-20 Thermo Fisher Scientific Oy Appareil et procédé permettant d'ouvrir et de fermer un récipient de réaction
US8540948B2 (en) 2010-12-23 2013-09-24 Eppendorf Ag Lidded container
JP6067577B2 (ja) 2010-12-23 2017-01-25 エッペンドルフ アクチエンゲゼルシャフトＥｐｐｅｎｄｏｒｆ ＡＧ 蓋付き容器
US8889086B2 (en) 2011-04-21 2014-11-18 Streck, Inc. Sample tube having particular utility for nucleic acid amplification
US9737891B2 (en) 2011-06-01 2017-08-22 Streck, Inc. Rapid thermocycler system for rapid amplification of nucleic acids and related methods
US20130309147A1 (en) * 2012-05-16 2013-11-21 Gene Era Biotech Co. Ltd. Sample tube with improved seal
US9932632B2 (en) 2012-08-10 2018-04-03 Streck, Inc. Real-time optical system for polymerase chain reaction
US9533803B1 (en) * 2013-06-04 2017-01-03 Csp Technologies, Inc. Container having a child resistant closure with a small profile
US10322856B2 (en) * 2013-06-04 2019-06-18 Csp Technologies, Inc. Container having a child resistant closure with a small profile
US9802196B2 (en) * 2013-03-13 2017-10-31 Alphagem Bio Inc. Ergonomic numbered connector to hold tubes with improved cap
DE102013209881A1 (de) 2013-05-28 2014-12-04 Leica Microsystems Cms Gmbh Lasermikrodissektionssystem mit Visualisierungseinrichtung, Visualisierungseinrichtung für Lasermikrodissektionssystem und Verfahren zur Lasermikrodissektion
DE102013209880A1 (de) 2013-05-28 2014-12-04 Leica Microsystems Cms Gmbh Verfahren zur Lasermikrodissektion und Lasermikrodissektionssystem
EP3014251A1 (fr) 2013-06-28 2016-05-04 Streck Inc. Dispositifs pour réaction en chaîne de polymérase en temps réel
WO2015094983A1 (fr) * 2013-12-20 2015-06-25 Retro Brands, Llc Distributeur de liquide à vapoter
EP3113882A1 (fr) 2014-03-04 2017-01-11 Streck Inc. Tube à échantillon amélioré à pointe transparente ayant une utilité particulière pour l'amplification d'acides nucléiques
EP2965816B1 (fr) 2014-07-09 2019-09-25 Eppendorf Ag Récipient réactionnel doté d'un couvercle
USD745113S1 (en) * 2014-09-23 2015-12-08 Farshad Fahim Air vent device
DE102015103634A1 (de) 2015-03-12 2016-09-29 Qiagen Gmbh Behälter zum Trocknen und Aufbereiten biologischer Proben
US20180133712A1 (en) 2015-05-26 2018-05-17 Københavns Universitet (Ku) Enzyme activity assay system and devices
US10889416B2 (en) 2017-04-24 2021-01-12 Csp Technologies, Inc. Child resistant container and method of opening same
US10961030B2 (en) 2017-04-24 2021-03-30 Csp Technologies, Inc. Slidably openable child resistant container
US10137447B1 (en) * 2017-05-17 2018-11-27 Biotix, Inc. Ergonomic fluid handling tubes
USD882113S1 (en) 2017-11-30 2020-04-21 Biotix, Inc. Fluid handling tube
USD881410S1 (en) 2018-01-19 2020-04-14 Biotix, Inc. Fluid handling tube
CA3140050C (fr) * 2019-05-17 2023-12-19 Solution Products, Llc Contenants pour leurres
US11454331B2 (en) * 2019-12-18 2022-09-27 Form Create Llc Valve cap assembly
CN112849730B (zh) * 2021-02-08 2024-08-13 江苏硕世生物科技股份有限公司 一种新型软盖、硬盖组合结构
USD1023958S1 (en) * 2022-03-31 2024-04-23 Alpha/Omega Energy Solutions, LLC Safety plug
USD1021801S1 (en) * 2022-03-31 2024-04-09 Alpha/Omega Energy Solutions, LLC Safety plug

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DE19645892A1 (de) * 1996-11-07 1998-05-14 Eppendorf Geraetebau Netheler Deckelgefäß
US5779074A (en) * 1994-07-26 1998-07-14 Becton, Dickinson And Company Combination stopper-shield closure

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DE29706611U1 (de) * 1997-04-12 1997-07-03 Drägerwerk AG, 23558 Lübeck Dichtung für eine Verschlußkappe
JP2001219949A (ja) * 2000-02-10 2001-08-14 Sekisui Chem Co Ltd 真空検体採取容器用栓体
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US5103990A (en) * 1990-10-29 1992-04-14 Hoover Universal, Inc. Closure for single service beverage container
US5513768A (en) * 1992-07-20 1996-05-07 Smith; James C. Sealing cap for containers
WO1995020527A1 (fr) * 1994-01-28 1995-08-03 Innervision, Inc. Bouchon de fermeture etanche pour recipients
US5779074A (en) * 1994-07-26 1998-07-14 Becton, Dickinson And Company Combination stopper-shield closure
DE19645892A1 (de) * 1996-11-07 1998-05-14 Eppendorf Geraetebau Netheler Deckelgefäß

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2468404A3 (fr) * 2010-12-23 2013-01-23 Eppendorf AG Récipient à couvercle
US11040806B2 (en) 2017-12-15 2021-06-22 Husky Injection Molding Systems Ltd. Closure cap for a container
EP3978116A1 (fr) * 2020-10-01 2022-04-06 Eppendorf AG Récipient à couvercle en matière plastique et procédé de marquage d'un récipient à couvercle en matière plastique
WO2022069360A1 (fr) * 2020-10-01 2022-04-07 Eppendorf Ag Récipient à couvercle en matière plastique, et procédé de marquage d'un récipient à couvercle en matière plastique

Also Published As

Publication number Publication date
US6783025B2 (en) 2004-08-31
ES2227376T3 (es) 2005-04-01
DE10159804A1 (de) 2003-06-26
DE50201344D1 (de) 2004-11-25
EP1317961B1 (fr) 2004-10-20
US20030102323A1 (en) 2003-06-05
ATE279986T1 (de) 2004-11-15
DE10159804B4 (de) 2004-09-16

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