EP1309854A2 - Colonne de chromatographie rapide - Google Patents

Colonne de chromatographie rapide

Info

Publication number
EP1309854A2
EP1309854A2 EP02704351A EP02704351A EP1309854A2 EP 1309854 A2 EP1309854 A2 EP 1309854A2 EP 02704351 A EP02704351 A EP 02704351A EP 02704351 A EP02704351 A EP 02704351A EP 1309854 A2 EP1309854 A2 EP 1309854A2
Authority
EP
European Patent Office
Prior art keywords
cartridge
cartridge housing
housing
chromatography
closed end
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP02704351A
Other languages
German (de)
English (en)
Inventor
Robert P. Ritacco
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Varian Inc
Original Assignee
Varian Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Varian Inc filed Critical Varian Inc
Publication of EP1309854A2 publication Critical patent/EP1309854A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6091—Cartridges
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/522—Physical parameters pressure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G01N30/6017—Fluid distributors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6034—Construction of the column joining multiple columns

Definitions

  • This invention leads to chromatographic columns and, or particularly, to process chromtography columns which have a disposable cartridge and thereby limit exposure of the operator to hazardous materials.
  • Chromatography is a well-known method of separating and analyzing mixtures of chemical substances.
  • a flow of a solvent containing a mixture causes the components of the mixture to migrate differentially in a porous, insoluble chromatographic sorbent.
  • Chromtography facilitates the purification, isolation and comparison of the components of mixtures of chemical substances.
  • a major component of a chromatography system is a chromatography column.
  • Prior art chromatography columns typically include a tube or housing which contains the chromatographic sorbent, an inlet at one end and an outlet at the opposite end.
  • the sorbent may be packed into the column under pressure.
  • a solvent containing a sample flows through the sorbent and is discharged through the outlet.
  • the column may include flow distribution devices for promoting uniform flow of the solvent through the sorbent. Examples of chromatographic columns are disclosed in U.S. Patent No. 4,582,608, issued April 15, 1986 to Ritacco, and U.S. Patent No. 4,737,292, issued April 12, 1988 to Ritacco, et al.
  • chromatography columns are used for purification of samples containing hazardous materials.
  • the hazardous materials remain in the sorbent after the purification process.
  • Prior art columns are typically filled with sorbent and are emptied by the operator after use. The operator is thus exposed to the solvent and the sorbent, as well as sorbent contaminated with potentially toxic chemicals after it has been used in a purification process. Such exposure is, of course, undesirable. The problem is particularly severe in the case of large production size chromatography columns.
  • a chromatography column assembly comprises a single-ended cartridge containing a chromatographic sorbent, the cartridge having an inlet, a column housing for receiving the cartridge, and a compression head for closing the cartridge, the compression head having an outlet.
  • a chromatography cartridge comprises a cartridge housing having an open end and a closed end, an inlet tube extending from the open end to the closed end of the cartridge housing, a flow distribution device located at the closed end of the cartridge housing and having a fluid connection to the inlet tube, and a chromatographic sorbent disposed within the interior volume of the cartridge housing.
  • the cartridge may further comprise first and second filter elements disposed at opposite ends of the chromatographic sorbent.
  • the cartridge housing may have a cylindrical geometry, and the inlet tube may be located at the center of the cartridge housing.
  • the chromatography column may further comprise one or more inflatable seals between the column housing and the cartridge.
  • a liquid may be disposed between the column housing and the cartridge.
  • the compression head may include a flow distribution device providing a fluid connection between the open end of the cartridge housing and the outlet.
  • the compression head may further include a seal for sealing the compression head to the cartridge housing and a seal for sealing the compression head to inlet tube.
  • a chromatography method comprises the steps of providing a cartridge housing containing a chromatographic sorbent, the cartridge housing having a open end and a closed end, causing a flow of a solvent through an inlet tube to the closed end of the cartridge housing, distributing the flow of the solvent from the inlet tube over the closed end of the cartridge housing, and causing a flow of the solvent through the chromatographic sorbent from the closed end to the open end of the cartridge housing.
  • the flow of solvent may be directed from the open end of the cartridge housing to an outlet.
  • FIG. 1 is a partially exploded cross-sectional diagram of a chromatography column in accordance with an embodiment of the invention.
  • FIG. 1 A chromatography column assembly 10 in accordance with an embodiment of the invention is shown in FIG. 1.
  • the chromatography column assembly 10 includes a column housing 12, a cartridge 14 positioned within column housing 12 and a compression head 16.
  • the compression head 16 is shown raised above cartridge 14 to facilitate an understanding of the structure. However, in use, compression head 16 is sealed to the upper end of cartridge 14, as described below.
  • the structure shown in FIG. 1 is particularly suited to large chromatography columns, but is not limited as to size.
  • the chromatography column preferably has a cylindrical geometry.
  • the column housing 12 includes a relatively thick and rigid base 20 and a cylindrical side wall 22.
  • the column housing 12 may, for example, be fabricated of stainless steel.
  • the column housing 12 has an open upper end 24.
  • Cartridge 14 includes a cartridge housing 30 having a cylindrical side wall 32, a bottom wall 34 that closes one end of housing 30 and an open upper end 36.
  • Cartridge housing 14 may be fabricated, for example, of stainless steel or ultra high molecular weight polyethylene and is dimensioned to provide a space 40 between side wall 22 of housing 12 and side wall 32 of cartridge housing 30.
  • each of column housing 12 and cartridge housing 30 is open on only one end and has the shape of a ba ⁇ el or drum.
  • a sintered filtered element 52, or frit, and a flow distribution device 54 are positioned at the bottom cartridge housing 30 adjacent to bottom wall 34.
  • An inlet tube 50 extends from just below the open upper end 36 of cartridge housing 30 to the flow distribution device 54.
  • Inlet tube 50 provides an inlet 48 of the cartridge 14.
  • Preferably, inlet tube 50 is centrally located in cartridge housing 30.
  • Filter element 52 is a sintered porous material having a desired porosity.
  • the flow distribution device 54 insures a relatively uniform flow of solvent into the chromatographic sorbent. Filter elements and flow distribution devices for chromatography columns are known in the art, as described, for example, in the aforementioned patent Nos. 4,582,608 and 4,737,292.
  • a support plate 56 may be positioned between bottom wall 34 of cartridge housing 30 and flow distribution device 54.
  • a chromatographic sorbent such as a silica gel, fills an interior volume 60 of cartridge housing 30 around inlet tube 50.
  • a filter element and holder 64 cover the top of the sorbent with enough space between the filter element and holder 64 and the open end 36 of the cartridge housing 30 to permit the compression head 16 to be installed as described below.
  • Filter element and holder 64 includes a central hole and seal appropriately sized to fit and seal to inlet tube 50.
  • Compression head 16 is configured to cover upper end 36 of cartridge housing 30 and to compress the sorbent within interior volume 60.
  • Compression head 16 includes a disk- shaped cover 70 having a seal 72 around its outer periphery. Cover 70 is dimensioned to fit within and seal upper end 36 of cartridge housing 30. Cover 70 further includes a central opening 74 for receiving inlet tube 50 and a seal 76 for forming a seal between cover 70 and inlet tube 50.
  • Compression head 16 further includes a flow distribution device 80 mounted to the lower surface of cover 70. The flow distribution device 80 directs solvent flow discharged through filter element and holder 64 to an outlet 82.
  • Compression head 16 further includes a structure 86 for transmitting a compression force, indicated by arrows 88, to cover 70 for compressing the sorbent in interior volume 60.
  • the cartridge 14 is placed within the column housing 12, and the compression head 16 is positioned within the upper end of cartridge 14 so as to seal the upper end 36 of cartridge housing 30.
  • a seal is formed between the outer periphery of cover 70 and the inside surface of cartridge housing 30.
  • inlet tube 50 extends through opening 74 and is sealed to cover 70.
  • Inflatable seals 90 located near the top and bottom of column housing 12, are activated, and an appropriate liquid, such as water, is placed in the space between cartridge housing 30 and column housing 12. Since liquids are nearly incompressible, this feature permits the cartridge 14 to be operated without material fatigue.
  • a sample to be purified and a solvent are introduced into the column through inlet 48.
  • the sample and the solvent flow downwardly through inlet tube 50, as indicated by arrow 92, to the lower end of cartridge 14.
  • the solvent and sample are distributed over the closed end of cartridge 14 by flow distribution device 54, as indicated by arrows 93, and then flow upwardly through the chromatographic sorbent, as indicated by arrows 94.
  • the solvent passes through filter element and holder 64 and is directed by flow distribution device 80 to outlet 82.
  • the compression head 16 is removed from cartridge 14 and cartridge 14 is removed from column housing 12, is capped and is discarded appropriately.
  • the configuration shown in FIG. 1 and described above permits the chromatography column to operate from one end and limits exposure of the operator to hazardous materials.
  • the cartridge 14 is disposable as a unit without any need to remove the sorbent from the cartridge housing 30. After a spent cartridge is removed, a new cartridge 14 can be placed in column housing 12.
  • the cartridge housing 30 has an inside diameter of 45 centimeters and is 38 centimeters long, thus providing a silica capacity of 35 kilograms for a silica density of 0.6 grams per cubic centimeter. It will be understood that these dimensions are given by way of example only and are not limiting as to the present invention. A variety of different configurations and dimensions may be utilized within the scope of the invention.

Abstract

L'invention concerne un ensemble colonne de chromatographie comprenant une cartouche à une seule extrémité renfermant une substance absorbante, un logement de colonne conçu pour recevoir la cartouche et une tête de compression servant à fermer ladite cartouche. Cette cartouche comprend un logement présentant une extrémité ouverte et une extrémité fermée, un tube d'admission, qui s'étend de l'extrémité ouverte vers l'extrémité fermée du logement de la cartouche, et présente un raccordement fluidique au tube d'admission, ainsi qu'une substance absorbante déposée dans le volume intérieur du logement de la cartouche. Des éléments filtrants peuvent être placés à des extrémités opposées de la substance absorbante. La tête de compression peut comprendre un dispositif répartiteur de flux fournissant un raccordement fluidique entre l'extrémité supérieure de la cartouche et une sortie.
EP02704351A 2001-02-02 2002-02-04 Colonne de chromatographie rapide Withdrawn EP1309854A2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26633801P 2001-02-02 2001-02-02
US266338P 2001-02-02
PCT/US2002/003343 WO2002063291A2 (fr) 2001-02-02 2002-02-04 Colonne de chromatographie rapide

Publications (1)

Publication Number Publication Date
EP1309854A2 true EP1309854A2 (fr) 2003-05-14

Family

ID=23014153

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02704351A Withdrawn EP1309854A2 (fr) 2001-02-02 2002-02-04 Colonne de chromatographie rapide

Country Status (5)

Country Link
EP (1) EP1309854A2 (fr)
JP (1) JP2004518960A (fr)
CA (1) CA2404879A1 (fr)
DE (1) DE1309854T1 (fr)
WO (1) WO2002063291A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101849184B (zh) 2007-12-05 2014-07-16 全技术联合公司 用于分析样本和收集样本流份的方法和装置
SG10201400369QA (en) 2008-12-04 2014-05-29 Alltech Associates Inc Methods and apparatus for moving aliquot samples of fluid
KR20110104004A (ko) 2008-12-10 2011-09-21 올테크 어소시에이츠, 인크. 크로마토그래피 시스템용 기계 판독형 태그 리더, 크로마토그래피 시스템의 분별 수집 시스템, 및 크로마토그래피 시스템
US8314934B2 (en) 2009-09-01 2012-11-20 Alltech Associates, Inc. Methods and apparatus for analyzing samples and collecting sample fractions
KR101736250B1 (ko) * 2014-07-30 2017-05-16 인하대학교 산학협력단 판형 크로마토그래피 칼럼

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6001253A (en) * 1995-05-16 1999-12-14 Dyax Corporation Liquid chromatography column

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO02063291A3 *

Also Published As

Publication number Publication date
CA2404879A1 (fr) 2002-08-15
DE1309854T1 (de) 2003-10-30
WO2002063291A3 (fr) 2003-03-13
JP2004518960A (ja) 2004-06-24
WO2002063291A2 (fr) 2002-08-15
WO2002063291A8 (fr) 2003-07-31

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