EP1299166A4 - Separation de polynucleotides sur supports de separation polymeres - Google Patents

Separation de polynucleotides sur supports de separation polymeres

Info

Publication number
EP1299166A4
EP1299166A4 EP00931999A EP00931999A EP1299166A4 EP 1299166 A4 EP1299166 A4 EP 1299166A4 EP 00931999 A EP00931999 A EP 00931999A EP 00931999 A EP00931999 A EP 00931999A EP 1299166 A4 EP1299166 A4 EP 1299166A4
Authority
EP
European Patent Office
Prior art keywords
separation media
polymeric separation
polynucleotide separations
separations
polynucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00931999A
Other languages
German (de)
English (en)
Other versions
EP1299166A1 (fr
Inventor
Douglas T Gjerde
Paul D Taylor
Robert M Haefele
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precipio Inc
Original Assignee
Transgenomic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgenomic Inc filed Critical Transgenomic Inc
Publication of EP1299166A1 publication Critical patent/EP1299166A1/fr
Publication of EP1299166A4 publication Critical patent/EP1299166A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B01J20/285—Porous sorbents based on polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287—Non-polar phases; Reversed phases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/80—Aspects related to sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J2220/82—Shaped bodies, e.g. monoliths, plugs, tubes, continuous beds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Saccharide Compounds (AREA)
EP00931999A 2000-05-01 2000-05-01 Separation de polynucleotides sur supports de separation polymeres Withdrawn EP1299166A4 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2000/011795 WO2001083072A1 (fr) 2000-05-01 2000-05-01 Separation de polynucleotides sur supports de separation polymeres

Publications (2)

Publication Number Publication Date
EP1299166A1 EP1299166A1 (fr) 2003-04-09
EP1299166A4 true EP1299166A4 (fr) 2003-07-09

Family

ID=21741337

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00931999A Withdrawn EP1299166A4 (fr) 2000-05-01 2000-05-01 Separation de polynucleotides sur supports de separation polymeres

Country Status (5)

Country Link
EP (1) EP1299166A4 (fr)
JP (1) JP2003532088A (fr)
AU (1) AU777251B2 (fr)
CA (1) CA2395101A1 (fr)
WO (1) WO2001083072A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6066258A (en) 1997-12-05 2000-05-23 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
US7169298B2 (en) 2000-01-26 2007-01-30 Transgenomic, Inc. Method and apparatus for separating polynucleotides using monolithic capillary columns
US20020086291A1 (en) * 2000-11-29 2002-07-04 Hornby David P. Methods and reagents for analysis of rna structure and function
WO2005078088A1 (fr) 2004-02-12 2005-08-25 Gl Sciences Incorporated Mécanisme de séparation et de purification d'adn et ainsi de suite
US8535527B2 (en) 2008-02-19 2013-09-17 Merck Patent Gmbh Monolithic supports and methods for their production
JP2012093092A (ja) * 2009-11-05 2012-05-17 Nagoya Institute Of Technology 平板型カラム、それを用いた分離システムおよび分離方法
TW201734026A (zh) * 2015-12-28 2017-10-01 昭和電工股份有限公司 分離分析法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998048914A1 (fr) * 1997-04-25 1998-11-05 Transgenomic, Inc. Milieu chromatographique liquide ameliore utilise dans la separation de polynucleotides
WO1998056797A1 (fr) * 1997-06-10 1998-12-17 Transgenomic, Inc. Systeme et procede permettant de realiser des separations de polynucleotides grace a la chromatographie en phase liquide
WO1999007899A1 (fr) * 1997-08-05 1999-02-18 Transgenomic, Inc. Chromatographie par denaturation d'ions apparies de polynucleotide permettant de detecter des mutations

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6066258A (en) * 1997-12-05 2000-05-23 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998048914A1 (fr) * 1997-04-25 1998-11-05 Transgenomic, Inc. Milieu chromatographique liquide ameliore utilise dans la separation de polynucleotides
WO1998056797A1 (fr) * 1997-06-10 1998-12-17 Transgenomic, Inc. Systeme et procede permettant de realiser des separations de polynucleotides grace a la chromatographie en phase liquide
WO1999007899A1 (fr) * 1997-08-05 1999-02-18 Transgenomic, Inc. Chromatographie par denaturation d'ions apparies de polynucleotide permettant de detecter des mutations

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO0183072A1 *

Also Published As

Publication number Publication date
CA2395101A1 (fr) 2001-11-08
WO2001083072A1 (fr) 2001-11-08
AU4979500A (en) 2001-11-12
JP2003532088A (ja) 2003-10-28
AU777251B2 (en) 2004-10-07
EP1299166A1 (fr) 2003-04-09

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