EP1027121A4 - Milieu chromatographique liquide ameliore utilise dans la separation de polynucleotides - Google Patents

Milieu chromatographique liquide ameliore utilise dans la separation de polynucleotides

Info

Publication number
EP1027121A4
EP1027121A4 EP98918701A EP98918701A EP1027121A4 EP 1027121 A4 EP1027121 A4 EP 1027121A4 EP 98918701 A EP98918701 A EP 98918701A EP 98918701 A EP98918701 A EP 98918701A EP 1027121 A4 EP1027121 A4 EP 1027121A4
Authority
EP
European Patent Office
Prior art keywords
liquid chromatographic
improved liquid
chromatographic media
polynucleotide separation
polynucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98918701A
Other languages
German (de)
English (en)
Other versions
EP1027121A1 (fr
Inventor
Douglas T Gjerde
Paul D Taylor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precipio Inc
Original Assignee
Transgenomic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgenomic Inc filed Critical Transgenomic Inc
Publication of EP1027121A1 publication Critical patent/EP1027121A1/fr
Publication of EP1027121A4 publication Critical patent/EP1027121A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/328—Polymers on the carrier being further modified
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287—Non-polar phases; Reversed phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3225—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating involving a post-treatment of the coated or impregnated product
    • B01J20/3227—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating involving a post-treatment of the coated or impregnated product by end-capping, i.e. with or after the introduction of functional or ligand groups
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
EP98918701A 1997-04-25 1998-04-24 Milieu chromatographique liquide ameliore utilise dans la separation de polynucleotides Withdrawn EP1027121A4 (fr)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US4485697P 1997-04-25 1997-04-25
US44856P 1997-04-25
US5545697P 1997-08-11 1997-08-11
US55456P 1997-08-11
US5952797P 1997-09-22 1997-09-22
US59527P 1997-09-22
US6212397P 1997-10-15 1997-10-15
US62123P 1997-10-15
US6230397P 1997-10-17 1997-10-17
US62303P 1997-10-17
US6361997P 1997-10-27 1997-10-27
US63619P 1997-10-27
US6931397P 1997-12-05 1997-12-05
US69313P 1997-12-05
US7799898P 1998-03-13 1998-03-13
US77998P 1998-03-13
US5833798A 1998-04-10 1998-04-10
US58337 1998-04-10
PCT/US1998/008293 WO1998048914A1 (fr) 1997-04-25 1998-04-24 Milieu chromatographique liquide ameliore utilise dans la separation de polynucleotides

Publications (2)

Publication Number Publication Date
EP1027121A1 EP1027121A1 (fr) 2000-08-16
EP1027121A4 true EP1027121A4 (fr) 2001-07-11

Family

ID=27578183

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98918701A Withdrawn EP1027121A4 (fr) 1997-04-25 1998-04-24 Milieu chromatographique liquide ameliore utilise dans la separation de polynucleotides

Country Status (5)

Country Link
EP (1) EP1027121A4 (fr)
JP (1) JP2002506425A (fr)
AU (1) AU725928B2 (fr)
CA (1) CA2285307A1 (fr)
WO (1) WO1998048914A1 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6475388B1 (en) 1996-11-13 2002-11-05 Transgenomic, Inc. Method and system for RNA analysis by matched ion polynucleotide chromatography
US6576133B2 (en) 1996-11-13 2003-06-10 Transgenomic, Inc Method and system for RNA analysis by matched ion polynucleotide chromatography
US6177559B1 (en) 1998-04-24 2001-01-23 Transgenomic, Inc. Process for separation of polynucleotide fragments
JP2001521729A (ja) * 1997-10-30 2001-11-13 トランスジエノミツク・インコーポレーテツド ポリヌクレオチドの高分離の液体クロマトグラフィー分離方法
US6258264B1 (en) 1998-04-10 2001-07-10 Transgenomic, Inc. Non-polar media for polynucleotide separations
US6265168B1 (en) 1998-10-06 2001-07-24 Transgenomic, Inc. Apparatus and method for separating and purifying polynucleotides
CA2395101A1 (fr) * 2000-05-01 2001-11-08 Transgenomic, Inc. Separation de polynucleotides sur supports de separation polymeres
JP2003534798A (ja) * 2000-05-26 2003-11-25 トランスジエノミツク・インコーポレーテツド ポリヌクレオチドを分離しそして精製するための装置及び方法
US6521411B2 (en) 2000-09-28 2003-02-18 Transgenomic, Inc. Method and system for the preparation of cDNA
JP4069999B2 (ja) * 2001-03-13 2008-04-02 独立行政法人理化学研究所 ナノ被覆分子性材料
DE10120821A1 (de) * 2001-04-27 2002-10-31 Sachtleben Chemie Gmbh Mit Polyalken beschichtete, poröse Füllstoffe
WO2003031580A2 (fr) 2001-10-05 2003-04-17 Transgenomic, Inc. Procedes et compositions d'analyse de mutation par chromatographie en phase liquide
CN103097887A (zh) * 2010-09-09 2013-05-08 株式会社大赛璐 用于分离水溶性生物物质的方法
EP2570183A1 (fr) * 2011-09-15 2013-03-20 InstrAction GmbH Sorbant comportant à sa surface une unité aliphatique pour la purification des molécules organiques
TW201726917A (zh) * 2015-12-28 2017-08-01 昭和電工股份有限公司 分離分析方法
CN109475840A (zh) * 2016-05-06 2019-03-15 黄金企业公司 吸附剂组合物、其制备方法及其用途

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4042327A (en) * 1976-01-15 1977-08-16 Waters Associates, Inc. Ion-pairing chromatography
US5015373A (en) * 1988-02-03 1991-05-14 Regents Of The University Of Minnesota High stability porous zirconium oxide spherules
US5100547A (en) * 1991-03-04 1992-03-31 Monsanto Company Chromatography supports consisting of an inorganic susbstrate coated with polybutadiene with alkyl side chains
WO1992015385A1 (fr) * 1991-03-04 1992-09-17 Simon Ethan S Revetement polymere en butadiene acrylonitrile et materiau de remplissage chromatographique
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0637510B2 (ja) * 1985-10-04 1994-05-18 東ソー株式会社 核酸の分離方法
AT398973B (de) * 1992-11-18 1995-02-27 Bonn Guenther Dr Verfahren zur trennung von nukleinsäuren

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4042327A (en) * 1976-01-15 1977-08-16 Waters Associates, Inc. Ion-pairing chromatography
US5015373A (en) * 1988-02-03 1991-05-14 Regents Of The University Of Minnesota High stability porous zirconium oxide spherules
US5100547A (en) * 1991-03-04 1992-03-31 Monsanto Company Chromatography supports consisting of an inorganic susbstrate coated with polybutadiene with alkyl side chains
WO1992015385A1 (fr) * 1991-03-04 1992-09-17 Simon Ethan S Revetement polymere en butadiene acrylonitrile et materiau de remplissage chromatographique
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ERIKSSON S ET AL: "SEPARATION OF DNA RESTRICTION FRAGMENTS BY ION-PAIR CHROMATOGRAPHY", JOURNAL OF CHROMATOGRAPHY,NL,ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, vol. 359, January 1986 (1986-01-01), pages 265 - 274, XP002910334, ISSN: 0021-9673 *
See also references of WO9848914A1 *

Also Published As

Publication number Publication date
JP2002506425A (ja) 2002-02-26
CA2285307A1 (fr) 1998-11-05
AU7157798A (en) 1998-11-24
EP1027121A1 (fr) 2000-08-16
AU725928B2 (en) 2000-10-26
WO1998048914A1 (fr) 1998-11-05

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