CA2285307A1 - Milieu chromatographique liquide ameliore utilise dans la separation de polynucleotides - Google Patents

Milieu chromatographique liquide ameliore utilise dans la separation de polynucleotides Download PDF

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Publication number
CA2285307A1
CA2285307A1 CA002285307A CA2285307A CA2285307A1 CA 2285307 A1 CA2285307 A1 CA 2285307A1 CA 002285307 A CA002285307 A CA 002285307A CA 2285307 A CA2285307 A CA 2285307A CA 2285307 A1 CA2285307 A1 CA 2285307A1
Authority
CA
Canada
Prior art keywords
bead
nonporous
beads
silica
polymer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002285307A
Other languages
English (en)
Inventor
Douglas T. Gierde
Paul D. Taylor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precipio Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2285307A1 publication Critical patent/CA2285307A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/328—Polymers on the carrier being further modified
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287—Non-polar phases; Reversed phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3225—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating involving a post-treatment of the coated or impregnated product
    • B01J20/3227—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating involving a post-treatment of the coated or impregnated product by end-capping, i.e. with or after the introduction of functional or ligand groups
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds

Abstract

L'invention porte sur des billes non poreuses dont le diamètre moyen est compris entre environ 0,5 et 100 microns et qui sont appropriées pour être utilisées dans la séparation chromatographique. Ces billes comprennent des particules non poreuses qui sont recouvertes d'un polymère dont presque tous les groupes de la surface du substrat sont coiffés aux extrémités par un groupe d'hydrocarbures non polaires ou substitués. Les billes assurent une séparation efficace des polynucléotides par chromatographie polynucléotidique à compatibilité ionique.
CA002285307A 1997-04-25 1998-04-24 Milieu chromatographique liquide ameliore utilise dans la separation de polynucleotides Abandoned CA2285307A1 (fr)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US4485697P 1997-04-25 1997-04-25
US60/044,856 1997-04-25
US5545697P 1997-08-11 1997-08-11
US60/055,456 1997-08-11
US5952797P 1997-09-22 1997-09-22
US60/059,527 1997-09-22
US6212397P 1997-10-15 1997-10-15
US60/062,123 1997-10-15
US6230397P 1997-10-17 1997-10-17
US60/062,303 1997-10-17
US6361997P 1997-10-27 1997-10-27
US60/063,619 1997-10-27
US6931397P 1997-12-05 1997-12-05
US60/069,313 1997-12-05
US7799898P 1998-03-13 1998-03-13
US60/077,998 1998-03-13
US5833798A 1998-04-10 1998-04-10
US09/058,337 1998-04-10
PCT/US1998/008293 WO1998048914A1 (fr) 1997-04-25 1998-04-24 Milieu chromatographique liquide ameliore utilise dans la separation de polynucleotides

Publications (1)

Publication Number Publication Date
CA2285307A1 true CA2285307A1 (fr) 1998-11-05

Family

ID=27578183

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002285307A Abandoned CA2285307A1 (fr) 1997-04-25 1998-04-24 Milieu chromatographique liquide ameliore utilise dans la separation de polynucleotides

Country Status (5)

Country Link
EP (1) EP1027121A4 (fr)
JP (1) JP2002506425A (fr)
AU (1) AU725928B2 (fr)
CA (1) CA2285307A1 (fr)
WO (1) WO1998048914A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017190251A1 (fr) * 2016-05-06 2017-11-09 Goldcorp Inc. Composition adsorbante, son procédé de fabrication et ses utilisations

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6475388B1 (en) 1996-11-13 2002-11-05 Transgenomic, Inc. Method and system for RNA analysis by matched ion polynucleotide chromatography
US6576133B2 (en) 1996-11-13 2003-06-10 Transgenomic, Inc Method and system for RNA analysis by matched ion polynucleotide chromatography
US6177559B1 (en) 1998-04-24 2001-01-23 Transgenomic, Inc. Process for separation of polynucleotide fragments
JP2001521729A (ja) * 1997-10-30 2001-11-13 トランスジエノミツク・インコーポレーテツド ポリヌクレオチドの高分離の液体クロマトグラフィー分離方法
US6258264B1 (en) 1998-04-10 2001-07-10 Transgenomic, Inc. Non-polar media for polynucleotide separations
US6265168B1 (en) 1998-10-06 2001-07-24 Transgenomic, Inc. Apparatus and method for separating and purifying polynucleotides
EP1299166A4 (fr) * 2000-05-01 2003-07-09 Transgenomic Inc Separation de polynucleotides sur supports de separation polymeres
CA2396667A1 (fr) * 2000-05-26 2001-12-06 Benjamin L. Legendre, Jr. Appareil et procede permettant la separation et la purification de polynucleotides
US6521411B2 (en) 2000-09-28 2003-02-18 Transgenomic, Inc. Method and system for the preparation of cDNA
JP4069999B2 (ja) * 2001-03-13 2008-04-02 独立行政法人理化学研究所 ナノ被覆分子性材料
DE10120821A1 (de) * 2001-04-27 2002-10-31 Sachtleben Chemie Gmbh Mit Polyalken beschichtete, poröse Füllstoffe
WO2003031580A2 (fr) 2001-10-05 2003-04-17 Transgenomic, Inc. Procedes et compositions d'analyse de mutation par chromatographie en phase liquide
US20130165632A1 (en) * 2010-09-09 2013-06-27 Atsushi Ohnishi Method for separating water-soluble biological substances
EP2570183A1 (fr) * 2011-09-15 2013-03-20 InstrAction GmbH Sorbant comportant à sa surface une unité aliphatique pour la purification des molécules organiques
TW201726917A (zh) * 2015-12-28 2017-08-01 昭和電工股份有限公司 分離分析方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4042327A (en) * 1976-01-15 1977-08-16 Waters Associates, Inc. Ion-pairing chromatography
JPH0637510B2 (ja) * 1985-10-04 1994-05-18 東ソー株式会社 核酸の分離方法
US5015373A (en) * 1988-02-03 1991-05-14 Regents Of The University Of Minnesota High stability porous zirconium oxide spherules
CA2102425A1 (fr) * 1991-03-04 1992-09-05 Ethan S. Simon Revetement a base de polymere de butadieneacrylonitrile et materiel de garnissage de colonne de chromatographie
US5100547A (en) * 1991-03-04 1992-03-31 Monsanto Company Chromatography supports consisting of an inorganic susbstrate coated with polybutadiene with alkyl side chains
AT398973B (de) * 1992-11-18 1995-02-27 Bonn Guenther Dr Verfahren zur trennung von nukleinsäuren
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017190251A1 (fr) * 2016-05-06 2017-11-09 Goldcorp Inc. Composition adsorbante, son procédé de fabrication et ses utilisations
CN109475840A (zh) * 2016-05-06 2019-03-15 黄金企业公司 吸附剂组合物、其制备方法及其用途
RU2739923C2 (ru) * 2016-05-06 2020-12-29 Голдкорп Инк. Композиция адсорбента, способ ее получения и ее применения
RU2739923C9 (ru) * 2016-05-06 2021-03-01 Голдкорп Инк. Адсорбирующая композиция, способ ее получения и ее применения
US11759767B2 (en) 2016-05-06 2023-09-19 Goldcorp Inc. Adsorbent composition, method of making the same, and uses thereof

Also Published As

Publication number Publication date
JP2002506425A (ja) 2002-02-26
EP1027121A1 (fr) 2000-08-16
AU7157798A (en) 1998-11-24
WO1998048914A1 (fr) 1998-11-05
EP1027121A4 (fr) 2001-07-11
AU725928B2 (en) 2000-10-26

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued