CA2395101A1 - Separation de polynucleotides sur supports de separation polymeres - Google Patents

Separation de polynucleotides sur supports de separation polymeres Download PDF

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Publication number
CA2395101A1
CA2395101A1 CA002395101A CA2395101A CA2395101A1 CA 2395101 A1 CA2395101 A1 CA 2395101A1 CA 002395101 A CA002395101 A CA 002395101A CA 2395101 A CA2395101 A CA 2395101A CA 2395101 A1 CA2395101 A1 CA 2395101A1
Authority
CA
Canada
Prior art keywords
acetate
separation
beads
column
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002395101A
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English (en)
Inventor
Douglas T. Gjerde
Paul D. Taylor
Robert M. Haefele
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precipio Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2395101A1 publication Critical patent/CA2395101A1/fr
Abandoned legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B01J20/285—Porous sorbents based on polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287—Non-polar phases; Reversed phases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/80—Aspects related to sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J2220/82—Shaped bodies, e.g. monoliths, plugs, tubes, continuous beds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Saccharide Compounds (AREA)

Abstract

Les supports de séparation polymères non polaires, tels que les billes ou les blocs, conviennent à la séparation chromatographique de mélanges de polynucléotides lorsque la surface desdits supports est substituée ou non par un groupe hydrocarboné comportant de un à 1 000 000 atomes de carbone et lorsque ladite surface est sensiblement exempte de toute contamination aux cations multivalents. Les supports polymères permettent, pour leur part, une séparation polynucléotidique efficace par chromatographie MIPC (Matched Ion Polynucleotide Chromatography). L'invention concerne également des procédés de conservation et de stockage desdits supports polymères, lesquels consistent à traiter lesdits supports avec des agents de liaison des cations multivalents.
CA002395101A 2000-05-01 2000-05-01 Separation de polynucleotides sur supports de separation polymeres Abandoned CA2395101A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2000/011795 WO2001083072A1 (fr) 2000-05-01 2000-05-01 Separation de polynucleotides sur supports de separation polymeres

Publications (1)

Publication Number Publication Date
CA2395101A1 true CA2395101A1 (fr) 2001-11-08

Family

ID=21741337

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002395101A Abandoned CA2395101A1 (fr) 2000-05-01 2000-05-01 Separation de polynucleotides sur supports de separation polymeres

Country Status (5)

Country Link
EP (1) EP1299166A4 (fr)
JP (1) JP2003532088A (fr)
AU (1) AU777251B2 (fr)
CA (1) CA2395101A1 (fr)
WO (1) WO2001083072A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6066258A (en) 1997-12-05 2000-05-23 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
US7169298B2 (en) 2000-01-26 2007-01-30 Transgenomic, Inc. Method and apparatus for separating polynucleotides using monolithic capillary columns
US20020086291A1 (en) * 2000-11-29 2002-07-04 Hornby David P. Methods and reagents for analysis of rna structure and function
JP4597870B2 (ja) 2004-02-12 2010-12-15 ジーエルサイエンス株式会社 Ｄｎａなどの分離精製機構
WO2009103399A2 (fr) * 2008-02-19 2009-08-27 Merck Patent Gmbh Supports monolithiques et leurs procédés de production
JP2012093092A (ja) * 2009-11-05 2012-05-17 Nagoya Institute Of Technology 平板型カラム、それを用いた分離システムおよび分離方法
TW201734026A (zh) * 2015-12-28 2017-10-01 昭和電工股份有限公司 分離分析法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography
US6066258A (en) * 1997-12-05 2000-05-23 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
AU725928B2 (en) * 1997-04-25 2000-10-26 Transgenomic, Inc. Improved liquid chromatographic media for polynucleotide separation
CA2288603A1 (fr) * 1997-06-10 1998-12-17 Robert M. Haefele Systeme et procede permettant de realiser des separations de polynucleotides grace a la chromatographie en phase liquide
AU750394B2 (en) * 1997-08-05 2002-07-18 Transgenomic, Inc. Denaturing multi ion polynucleotide chromatography for detecting mutations

Also Published As

Publication number Publication date
JP2003532088A (ja) 2003-10-28
WO2001083072A1 (fr) 2001-11-08
EP1299166A1 (fr) 2003-04-09
EP1299166A4 (fr) 2003-07-09
AU777251B2 (en) 2004-10-07
AU4979500A (en) 2001-11-12

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued