AU777251B2 - Polynucleotide separations on polymeric separation media - Google Patents

Polynucleotide separations on polymeric separation media Download PDF

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Publication number
AU777251B2
AU777251B2 AU49795/00A AU4979500A AU777251B2 AU 777251 B2 AU777251 B2 AU 777251B2 AU 49795/00 A AU49795/00 A AU 49795/00A AU 4979500 A AU4979500 A AU 4979500A AU 777251 B2 AU777251 B2 AU 777251B2
Authority
AU
Australia
Prior art keywords
monolith
separation
polymeric
beads
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU49795/00A
Other languages
English (en)
Other versions
AU4979500A (en
Inventor
Douglas T. Gjerde
Robert M. Haefele
Paul D. Taylor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precipio Inc
Original Assignee
Transgenomic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgenomic Inc filed Critical Transgenomic Inc
Publication of AU4979500A publication Critical patent/AU4979500A/en
Application granted granted Critical
Publication of AU777251B2 publication Critical patent/AU777251B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B01J20/285—Porous sorbents based on polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287—Non-polar phases; Reversed phases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/80—Aspects related to sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J2220/82—Shaped bodies, e.g. monoliths, plugs, tubes, continuous beds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Saccharide Compounds (AREA)
AU49795/00A 2000-05-01 2000-05-01 Polynucleotide separations on polymeric separation media Ceased AU777251B2 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2000/011795 WO2001083072A1 (fr) 2000-05-01 2000-05-01 Separation de polynucleotides sur supports de separation polymeres

Publications (2)

Publication Number Publication Date
AU4979500A AU4979500A (en) 2001-11-12
AU777251B2 true AU777251B2 (en) 2004-10-07

Family

ID=21741337

Family Applications (1)

Application Number Title Priority Date Filing Date
AU49795/00A Ceased AU777251B2 (en) 2000-05-01 2000-05-01 Polynucleotide separations on polymeric separation media

Country Status (5)

Country Link
EP (1) EP1299166A4 (fr)
JP (1) JP2003532088A (fr)
AU (1) AU777251B2 (fr)
CA (1) CA2395101A1 (fr)
WO (1) WO2001083072A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6066258A (en) 1997-12-05 2000-05-23 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
EP1235852B1 (fr) 2000-01-26 2011-06-15 TRANSGENOMIC, Inc. Procede permettant de separer des polynucleotides a l'aide de colonnes capillaires monolithiques
US20020086291A1 (en) * 2000-11-29 2002-07-04 Hornby David P. Methods and reagents for analysis of rna structure and function
EP1719816B1 (fr) * 2004-02-12 2011-06-01 GL Sciences Incorporated Mecanisme de separation et de purification d 'adn
WO2009103399A2 (fr) * 2008-02-19 2009-08-27 Merck Patent Gmbh Supports monolithiques et leurs procédés de production
JP2012093092A (ja) * 2009-11-05 2012-05-17 Nagoya Institute Of Technology 平板型カラム、それを用いた分離システムおよび分離方法
TW201734026A (zh) * 2015-12-28 2017-10-01 昭和電工股份有限公司 分離分析法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography
US6066258A (en) * 1997-12-05 2000-05-23 Transgenomic, Inc. Polynucleotide separations on polymeric separation media

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2285307A1 (fr) * 1997-04-25 1998-11-05 Transgenomic, Inc. Milieu chromatographique liquide ameliore utilise dans la separation de polynucleotides
DE69817207T2 (de) * 1997-06-10 2004-06-17 Transgenomic, Inc., San Jose System und verfahren zur durchführung von polynukleotid-trennungen mit hilfe von flüssigchromatographie
EP1002137A4 (fr) * 1997-08-05 2003-01-02 Transgenomic Inc Chromatographie par denaturation d'ions apparies de polynucleotide permettant de detecter des mutations

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography
US6066258A (en) * 1997-12-05 2000-05-23 Transgenomic, Inc. Polynucleotide separations on polymeric separation media

Also Published As

Publication number Publication date
CA2395101A1 (fr) 2001-11-08
WO2001083072A1 (fr) 2001-11-08
EP1299166A4 (fr) 2003-07-09
AU4979500A (en) 2001-11-12
EP1299166A1 (fr) 2003-04-09
JP2003532088A (ja) 2003-10-28

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