EP1240329A2 - ANTIG NES D RIV S DE i STREPTOCOCCUS PNEUMONIAE /i - Google Patents
ANTIG NES D RIV S DE i STREPTOCOCCUS PNEUMONIAE /iInfo
- Publication number
- EP1240329A2 EP1240329A2 EP00977771A EP00977771A EP1240329A2 EP 1240329 A2 EP1240329 A2 EP 1240329A2 EP 00977771 A EP00977771 A EP 00977771A EP 00977771 A EP00977771 A EP 00977771A EP 1240329 A2 EP1240329 A2 EP 1240329A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- protein
- pneumoniae
- antigenic fragment
- nucleic acid
- sds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241000193998 Streptococcus pneumoniae Species 0.000 title claims abstract description 16
- 229940031000 streptococcus pneumoniae Drugs 0.000 title claims abstract description 16
- 239000000427 antigen Substances 0.000 title description 8
- 102000036639 antigens Human genes 0.000 title description 8
- 108091007433 antigens Proteins 0.000 title description 8
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 137
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 137
- 230000000890 antigenic effect Effects 0.000 claims abstract description 26
- 208000015181 infectious disease Diseases 0.000 claims abstract description 15
- 229960005486 vaccine Drugs 0.000 claims abstract description 13
- 238000003745 diagnosis Methods 0.000 claims abstract description 11
- 238000002360 preparation method Methods 0.000 claims abstract description 11
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- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims description 21
- 102000039446 nucleic acids Human genes 0.000 claims description 14
- 108020004707 nucleic acids Proteins 0.000 claims description 14
- 150000007523 nucleic acids Chemical class 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 12
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- 239000002671 adjuvant Substances 0.000 claims description 7
- 238000005571 anion exchange chromatography Methods 0.000 claims description 7
- 238000011321 prophylaxis Methods 0.000 claims description 7
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- IOUPEELXVYPCPG-UHFFFAOYSA-N Valylglycine Chemical compound CC(C)C(N)C(=O)NCC(O)=O IOUPEELXVYPCPG-UHFFFAOYSA-N 0.000 claims description 6
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- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 claims description 3
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- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 9
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- YYPNLXNMXQFMHG-GSEHKNNPSA-N (3r,5s,8s,9s,10s,13s,14s,17s)-17-acetyl-3-hydroxy-10,13-dimethyl-1,2,3,4,5,6,7,8,9,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-11-one;[2-[(3r,5s,8s,9s,10s,13s,14s,17s)-3-hydroxy-10,13-dimethyl-11-oxo-1,2,3,4,5,6,7,8,9,12,14,15,16,17-tetradecahyd Chemical compound C([C@@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H](C(=O)C)[C@@]2(C)CC1=O.C([C@@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H](C(=O)COC(=O)C)[C@@]2(C)CC1=O YYPNLXNMXQFMHG-GSEHKNNPSA-N 0.000 description 2
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- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 2
- 230000005875 antibody response Effects 0.000 description 2
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- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
- 239000007195 tryptone soya broth Substances 0.000 description 2
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- VMXLZAVIEYWCLQ-UHFFFAOYSA-N 4-(4-aminophenyl)-3-methylaniline Chemical compound CC1=CC(N)=CC=C1C1=CC=C(N)C=C1 VMXLZAVIEYWCLQ-UHFFFAOYSA-N 0.000 description 1
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- TYJOQICPGZGYDT-UHFFFAOYSA-N 4-methylsulfonylbenzenesulfonyl chloride Chemical compound CS(=O)(=O)C1=CC=C(S(Cl)(=O)=O)C=C1 TYJOQICPGZGYDT-UHFFFAOYSA-N 0.000 description 1
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- 102100026413 Branched-chain-amino-acid aminotransferase, mitochondrial Human genes 0.000 description 1
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- 241001112695 Clostridiales Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
- YQEZLKZALYSWHR-UHFFFAOYSA-N Ketamine Chemical compound C=1C=CC=C(Cl)C=1C1(NC)CCCCC1=O YQEZLKZALYSWHR-UHFFFAOYSA-N 0.000 description 1
- 206010024971 Lower respiratory tract infections Diseases 0.000 description 1
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- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 1
- 206010033078 Otitis media Diseases 0.000 description 1
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- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
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- WEXRUCMBJFQVBZ-UHFFFAOYSA-N pentobarbital Chemical compound CCCC(C)C1(CC)C(=O)NC(=O)NC1=O WEXRUCMBJFQVBZ-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
- C07K14/3156—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci from Streptococcus pneumoniae (Pneumococcus)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/16—Otologicals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56905—Protozoa
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/315—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Streptococcus (G), e.g. Enterococci
Definitions
- FIGURE 1 is a flow chart with a schematic summary of the protein purification procedure used in the present invention.
- FIGURE 6 is a histogram showing pulmonary clearance following immunisation with S. pneumoniae protein of 34 kDa.
- the lungs were removed following lavage, placed in 2mL sterile PBS and homogenised.
- the lung homogenate was assessed by plating 10-fold serial dilutions onto blood agar for CFU determination. Results are presented only for the proteins which showed significant degrees of pulmonary clearance from the lungs.
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Abstract
L'invention se rapporte à une protéine antigénique dérivée de Streptococcus pneumoniae. Cette protéine antigénique s'avère utile s'agissant de la préparation de vaccins et du diagnostic de l'infection par Streptococcus pneumoniae.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9928678 | 1999-12-03 | ||
GBGB9928678.3A GB9928678D0 (en) | 1999-12-03 | 1999-12-03 | Streptococcus pneumoniae antigens |
PCT/GB2000/004602 WO2001040472A2 (fr) | 1999-12-03 | 2000-12-01 | Antigènes dérivés de streptococcus pneumoniae |
Publications (1)
Publication Number | Publication Date |
---|---|
EP1240329A2 true EP1240329A2 (fr) | 2002-09-18 |
Family
ID=10865707
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP00977771A Withdrawn EP1240329A2 (fr) | 1999-12-03 | 2000-12-01 | ANTIG NES D RIV S DE i STREPTOCOCCUS PNEUMONIAE /i |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1240329A2 (fr) |
JP (1) | JP2003515341A (fr) |
AU (1) | AU1540501A (fr) |
CA (1) | CA2392895A1 (fr) |
GB (1) | GB9928678D0 (fr) |
WO (1) | WO2001040472A2 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2261241A1 (fr) | 2001-04-16 | 2010-12-15 | Wyeth Holdings Corporation | Cadres de lecture ouverts de streptococcus pneumoniae codant pour des antigenes polypeptidiques, et leurs utilisations |
WO2011112906A2 (fr) | 2010-03-12 | 2011-09-15 | Children's Medical Center Corporation | Nouveaux immunogènes et procédés pour la découverte et le dépistage de ceux-ci |
ES2959258T3 (es) | 2013-02-07 | 2024-02-22 | Childrens Medical Center | Antígenos proteicos que proporcionan protección contra la colonización y/o la enfermedad neumocócicas |
BR112021004193A2 (pt) | 2018-09-12 | 2021-05-25 | Affinivax, Inc. | vacinas pneumocócicas multivalentes |
Family Cites Families (1)
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US5474905A (en) * | 1993-11-24 | 1995-12-12 | Research Corporation Technologies | Antibodies specific for streptococcus pneumoniae hemin/hemoglobin-binding antigens |
-
1999
- 1999-12-03 GB GBGB9928678.3A patent/GB9928678D0/en not_active Ceased
-
2000
- 2000-12-01 EP EP00977771A patent/EP1240329A2/fr not_active Withdrawn
- 2000-12-01 JP JP2001542537A patent/JP2003515341A/ja active Pending
- 2000-12-01 CA CA002392895A patent/CA2392895A1/fr not_active Abandoned
- 2000-12-01 AU AU15405/01A patent/AU1540501A/en not_active Abandoned
- 2000-12-01 WO PCT/GB2000/004602 patent/WO2001040472A2/fr not_active Application Discontinuation
Non-Patent Citations (1)
Title |
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See references of WO0140472A2 * |
Also Published As
Publication number | Publication date |
---|---|
WO2001040472A3 (fr) | 2002-03-14 |
JP2003515341A (ja) | 2003-05-07 |
WO2001040472A2 (fr) | 2001-06-07 |
AU1540501A (en) | 2001-06-12 |
GB9928678D0 (en) | 2000-02-02 |
CA2392895A1 (fr) | 2001-06-07 |
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