EP1212598A2 - Procede et dispositif pour l'analyse de gaz quantitative - Google Patents

Procede et dispositif pour l'analyse de gaz quantitative

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Publication number
EP1212598A2
EP1212598A2 EP00967587A EP00967587A EP1212598A2 EP 1212598 A2 EP1212598 A2 EP 1212598A2 EP 00967587 A EP00967587 A EP 00967587A EP 00967587 A EP00967587 A EP 00967587A EP 1212598 A2 EP1212598 A2 EP 1212598A2
Authority
EP
European Patent Office
Prior art keywords
measuring
measuring chamber
radiation
sample
adapter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00967587A
Other languages
German (de)
English (en)
Inventor
Holger Müller
Udo Schmale
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP1212598A2 publication Critical patent/EP1212598A2/fr
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/497—Physical analysis of biological material of gaseous biological material, e.g. breath
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/497—Physical analysis of biological material of gaseous biological material, e.g. breath
    • G01N33/4977—Metabolic gas from microbes, cell cultures or plant tissues

Definitions

  • the invention relates to a method and a device for quantitative gas analysis with the features of the preamble of claim 1 and of the claim.
  • a gas sample is taken from a sample bottle.
  • the CO 2 concentration is then determined using gas chromatography.
  • this procedure is very labor-intensive, and errors can occur when transferring the gas sample into the gas chromatograph.
  • the atmosphere above the sample is influenced by gas sampling.
  • the sampling syringe must be decontaminated and disposed of after each sampling.
  • a gas sample is taken from the sample bottle using a closed pump system that contains a gas analyzer.
  • the atmosphere of the sample bottle is also changed.
  • the closed pump system When examining several samples, the closed pump system must be decontaminated in a technically complex manner after each measurement.
  • the sample bottle is placed in the beam path of an infrared absorption measuring unit.
  • the C0 2 concentration is determined by the attenuation of the radiation at a characteristic wavelength, for example 4.24 ⁇ m.
  • the measurement result can be falsified by condensed moisture. Shaking the bottles can contaminate the inside of the bottle, which in turn affects the measurement. Therefore quantitative measurements are only possible with high technical effort and high costs.
  • the invention is therefore based on the object of creating a generic method and a device for carrying out the method, so that gas lyses and especially quantitative measurements of gas concentrations can be carried out permanently, trouble-free and inexpensively.
  • the object is achieved according to the invention in the above-mentioned generic method in that at least one radiation source and at least one detector device are fixed to the measuring chamber in a defined orientation and that the measuring radiation emitted by the radiation source passes through the measuring chamber at least once and after exiting the measuring chamber is detected by the detector device.
  • the sample atmosphere to be measured from the sample system containing a sample which has reached the separate measuring chamber by diffusion, is passed through by the measuring radiation in the measuring chamber at least once.
  • Neither a removal of the gas sample itself nor a measuring or detection device within the measuring chamber is required.
  • the diffusion connection takes place with a seal against the ambient atmosphere, so that short-term as well as continuous, longer-lasting measurements are reliably possible without interference from moisture or dirt.
  • the establishment of the diffusion connection between the sampling atmosphere contained in a sample system and a measuring chamber via a measuring adapter that can be separated from the sensor device is not limited to a specific sample system; many types of different sample systems such as sample bottles or crimp neck bottles are suitable, but also open sample systems such as pipes and pipes the like, for a measurement in the context of the method according to the invention.
  • the procedure allows Order of different sensor devices for different measuring adapters and measuring chambers.
  • a diffusion line, a cannula or simple openings which are formed in a measuring chamber bottom of a measuring chamber fitted as a stopper in a bottle neck, can be used.
  • the measurement itself is carried out after setting the diffusion equilibrium between the gas in the measuring chamber and the sample atmosphere.
  • the method offers a simple and inexpensive way of analyzing the sample atmosphere quantitatively. Elaborate pumping devices and the like, which could be used repeatedly, but would have to be decontaminated after each use, are not required.
  • the preferably one-time use of the measuring adapter (disposable article), which can be produced inexpensively as a mass article, prevents cross-contamination of different samples.
  • the measuring adapter can be heated with a required amount of heat.
  • the radiation source and the detector device can be defined in a defined orientation on the measuring chamber, that the measuring chamber of at least one for measuring radiation of the radiation source permeable cover is limited, and that the measuring radiation emitted by the radiation source after passage through the measuring chamber is detected by the detector device.
  • This device does not require any sensors or other measuring devices in the measuring chamber, since outside the measuring chamber a change in the measuring radiation is detected after it has passed through the measuring chamber.
  • the defined arrangement of the radiation source and the detector device on the measuring chamber ensures an exact, reproducible measurement result, it being expedient if the measuring chamber is contained in a measuring adapter which can be attached to the sample system.
  • the device it is possible to create a diffusion connection to any sample system by means of a simple, inexpensive measuring adapter, so that the respective sample atmosphere can get into the measuring chamber without taking the gas sample from the sample system.
  • the sample atmosphere is kept ready separated from the ambient atmosphere and can be measured and quantitatively analyzed by the separate sensor device arranged outside the measuring chamber.
  • the radiation source and the detector device are arranged in a sensor head which can be coupled to the measuring adapter. Thanks to the removable sensor head, many identical or different sample systems or sample atmospheres can be examined with just one sensor head.
  • the measuring adapter can permanently, e.g. B. stay in diffusion contact with the sample system for several weeks, the measurement can be carried out continuously or discontinuously with a sensor head.
  • the measuring adapter can preferably contain the measuring chamber. It is also expedient that the radiation source is contained in the measuring adapter, also together with the measuring chamber.
  • the measuring adapter has a universal connection for different sample systems.
  • Such a measuring adapter can be used as a disposable measuring adapter due to its inexpensive manufacture. Complicated decontamination of the measuring device is therefore eliminated.
  • the measuring adapter can also be used on open sample systems, e.g. Pipelines are coupled.
  • the measuring chamber can be designed differently and can be arranged in different positions with respect to the radiation source and the detector device.
  • the measuring chamber contains a first radiation-permeable cover or disk at the entrance of the measuring radiation into the measuring chamber and a second radiation-permeable cover at the exit of the measuring radiation from the measuring chamber.
  • the measuring radiation enters the measuring chamber through the first disk and leaves it after passing through the second cover in the direction of the detector device. If the first cover and the second cover on the measuring chamber are arranged approximately opposite one another, the measuring radiation can traverse the measuring chamber in a straight path, the measuring chamber in particular between see the radiation source and the detector device can be arranged.
  • the measuring radiation can also be directed by optical elements from the radiation source to the measuring chamber and / or from the measuring chamber to the detector device, so that different arrangements of the radiation source and the detector device can be selected.
  • the measuring chamber is delimited on the one hand by the transparent cover adjacent to the radiation source and the detector device and on the other hand by a measuring chamber wall reflecting the measuring radiation, so that the measuring radiation emitted by the radiation source is reflected after passing through the measuring chamber to the detector device.
  • the radiation source and the detector device can be arranged in the sensor head next to one another with an approximately parallel measurement beam exit from the radiation source and measurement beam input into the detector device.
  • the measuring chamber opens in a funnel or pyramid shape to a coupled sensor head and the measuring chamber walls reflect the measuring radiation. This results in a double reflection of the measuring radiation on the opposite funnel walls.
  • the reflecting measuring chamber wall is a lower reflection plate with openings as a diffusion connection parallel to the upper cover.
  • the radiation source and the detector device can be arranged at an angle to one another, so that the Measurement radiation is directed from the reflection plate directly to the detector device.
  • the measuring adapter is designed as a stopper for a sample bottle, which can be inserted in particular into a bottle neck of the sample bottle.
  • a sample bottle is e.g. B. a standardized crimp neck bottle.
  • the length of the measuring chamber can be comparatively long, so that gases with a low absorption coefficient can be monitored quantitatively due to the long path of the measuring radiation through the measuring chamber.
  • a diffusion connection can be formed in the measuring chambers described in that the reflecting measuring chamber wall or reflection plate has at least one opening, the opening diameter being a determining factor for the time period for setting the diffusion equilibrium.
  • the sample system or the sample bottle is closed with an elastomeric closure and the diffusion connection of the measuring adapter is a cannula for penetrating the closure.
  • the size or diameter of the measuring chamber is not dependent on or limited by the size of the bottle neck. In order to achieve the equilibrium between the sample atmosphere and the gas atmosphere in the measuring chamber in the shortest possible time, it is advisable to make the diameter of the cannula as large as possible, its length as short as possible and the volume of the measuring chamber as small as possible. The optimized dimensions are determined by the kinetics of the sample to be examined. IC
  • one source can be used as a reference to compensate for the aging of the other radiation sources, since it is not used as often and the aging is therefore negligible. In principle, this is possible with any gas concentration.
  • the measuring process can be carried out after a z.
  • B. automatic switchover to the second radiation source can be continued essentially without interruption.
  • the sensor device can have at least two detector devices, so that a reference measurement can be carried out simultaneously.
  • the radiation source irradiates both detectors to the same extent (same light path), one detector providing a concentration-dependent signal in the presence of the gas concentration to be measured, while the other detector serves only as a reference and therefore does not provide a concentration-dependent signal.
  • the measuring adapter and the sensor head which are designed as separate components of the device according to the invention, are expediently brought into a defined position with respect to one another via their housing or an integrated positioning unit for carrying out a measurement and then mechanically stably connected to one another by means of a coupling device. It is advantageous if the coupling device is provided essentially on the sensor head, since in In this case, the measuring adapter is constructed more simply and can be manufactured more cost-effectively.
  • the coupling device can also be arranged exclusively on the sensor head or can be a separate component.
  • a broadband thermal emitter LEDs (light emitting diodes), diode lasers and in particular infrared emitters or UV light emitters can be provided as the radiation source.
  • the radiation-permeable cover or pane can consist of soda-lime glass, borosilicate glass, quartz glass, silicon or sapphire, calcium fluoride (CaF 2 ), barium fluoride (BaF 2 ), germanium (Ge) or zinc selenide (ZnSe).
  • sensor heads that are equipped with different sensor devices can be provided for coupling to the measuring adapter or adapters.
  • the gas concentration measurement by a sensor device in the measuring chamber is preferably carried out by means of gas-specific absorption of electromagnetic radiation.
  • the measuring adapter is designed such that the electromagnetic radiation emitted by the sensor head in the measuring chamber interacts with the diffused sample atmosphere and can then be detected by the sensor head.
  • the simple and mass-producible form of the measuring adapter ters e.g. B. as a plastic injection molded part, after a one-time type calibration of the sensor head with a measuring adapter, no further calibration for identical measuring adapters is necessary.
  • Figure 1 is a side view in section and in cal atic representation of an inventive device on a sample bottle.
  • FIG. 2 shows a sectional view of an exemplary embodiment of the device according to the invention
  • FIG. 3 shows the measuring adapter of the device in a sectional view along the plane A-A in FIG. 2;
  • FIG. 4 shows a further embodiment of the measuring adapter of the device in a sectional view along the plane A-A in FIG. 2;
  • FIG. 5 shows a sectional view of a further exemplary embodiment of the device according to the invention.
  • FIG. 6 shows the measuring adapter of the device in a sectional view along the plane B-B in FIG. 5;
  • FIG. 7 shows a top view of the device with a coupling device; 8 shows a sectional view of a further exemplary embodiment of the device according to the invention;
  • FIG. 10 is a top plan view of an exemplary embodiment of the measuring adapter of the device.
  • FIG. 11 is a top plan view of a further exemplary embodiment of the measuring adapter of the device.
  • FIG. 13 shows a bottom view of an exemplary embodiment of the sensor head of the device
  • 15 is a bottom view of a further embodiment of the sensor head of the device.
  • FIG 16 shows a side view in section of a further exemplary embodiment, in which the measuring adapter contains the radiation source.
  • Fig. 17 in a sectional view a further embodiment in which the measuring adapter a pipe is attached.
  • FIG. 18 shows a measurement curve of the device according to the invention for monitoring the CO 2 production of a yeast culture.
  • a solid, semi-solid, liquid or gaseous sample 1 to be examined is located in a sample bottle 2, which is sealed with an elastomeric closure, e.g. B. a septum 10, and a crimp cap 11 attached to the bottle neck 21 is firmly closed.
  • a sample atmosphere 3 forms above sample 1 within sample bottle 2.
  • An exemplary embodiment of a device for quantitative gas analysis contains a measuring adapter 4 and a sensor head 5, which is connected by a flexible cable 6 to an electronic measuring and control device 7.
  • the measuring adapter 4 has a housing 8 with which it can be fixed on the sample bottle 2, as well as a measuring chamber 9 formed in the housing 8 and a cannula 20 connected to the measuring chamber 9.
  • the cannula 20 After piercing the septum 10, establishes a connection between the interior of the sample bottle 2 and the measuring chamber 9, so that the sample atmosphere 3 can reach the measuring chamber 9 by means of diffusion.
  • the gas atmosphere within the measuring chamber 9 is thus in a diffusion equilibrium with the sample atmosphere 3 in the sample bottle 2.
  • the time ⁇ required for the establishment of the equilibrium is essentially determined by the length and the transverse Cut surface of the cannula 20 and the volume of the measuring chamber 9 determined. In order to ensure that the change in the sample composition is recorded over time, the time ⁇ must be less than the changes over time in the sample composition. This condition is taken into account in the design of the cannula 20 and the measuring chamber 9.
  • the measurement of the gas concentration in the measuring chamber 9 takes place with the aid of the sensor head 5, which has a coupling device 14 such as, for. B. has a screw connection shown in Fig. 1, which ensures a firm connection between the sensor head 5 and the measuring adapter 4.
  • the sensor head 5 contains a housing 12 with a sensor device 13, which is assigned to the measuring chamber 9 and enables continuous or quasi-continuous contactless measurement and monitoring of the concentration and composition of the gas atmosphere in the measuring chamber 9, from which conclusions can be drawn about the properties of sample 1 in the sample bottle 2 can be pulled.
  • Fig. 2 shows an embodiment of the measuring chamber 9 and the sensor device 13 of the device according to the invention.
  • the sensor device 13 contains a radiation source 16 for generating electromagnetic radiation in the relevant spectral range and a detector device 17 for detecting the residual radiation after passing through the measuring chamber 9.
  • Die Radiation source 16 and detector device 17 are constructed in such a way that preferably only the selective wavelength-specific attenuation of the radiation intensity due to the interaction with the gas molecules to be detected in the measuring chamber 9 will measure.
  • a wavelength-selecting element for. B. an optical filter.
  • the measuring chamber 9 is covered on its upper side facing the detector device 17 with a window which is permeable to the measuring radiation, in particular an optical window 15, which is sealed or fixed on the housing 8 with a seal 18, which can also be an adhesive.
  • the optical window 15 consists of a material that is transparent in the relevant spectral range.
  • the window 15 can e.g. B. consist of a piece of monocrystalline silicon and can also have an anti-reflective layer.
  • the inner walls 22 of the measuring chamber 9 are shaped and machined in such a way that reflection and continuation of the radiation emitted by the radiation source 16 to the detector device 17 is ensured.
  • the reflecting walls 22 of the measuring chamber 9 can be arranged at an angle of 45 ° to the direction of the emitted and reflected radiation 24, as shown in FIG. 2.
  • the z. B. are formed as mutually associated circumferential shoulders 23, a releasable, yet mechanically strong, stable and reproducible alignment and positioning of the sensor head 5 on the measuring adapter 4 and thus the sensor device 13 on the measuring chamber 9 is achieved. From A high quality mechanical processing of the contacting surfaces resulting precision of this coupling ensures quantitative gas concentration measurements in the measuring chamber 9 even after repeated coupling / uncoupling of the sensor head 5 to the measuring adapter 4.
  • FIG. 3 shows the measuring adapter 4 according to FIG. 2 with a conical or funnel-shaped measuring chamber 9, in which the cone angle of the walls 22 is 90 ° and which is covered by a round window 15.
  • FIG. 4 shows a further embodiment of the measuring adapter 4 according to FIG. 2 with a measuring chamber 9, rectangular in plan view on the upper side, with two plane walls 22 reflecting the incident radiation 24 to the detector device 17 in a wedge shape at a wedge angle of 90 ° to one another.
  • the measuring chamber 9 is covered by a rectangular window 15.
  • the radiation source 16 of the sensor device 13 and the detector device 17 are spaced apart from one another on an optical axis 25 and arranged opposite one another.
  • the measuring chamber 9 is tubular and is gas-tightly covered by two opposite optical windows 15. If the sensor head 5 is arranged on the measuring adapter 4, the measuring chamber 9 is between the
  • FIG. 6 shows the housing 8 of the measuring adapter 4 according to FIG. 5 with the tubular measuring chamber 9 and the two windows 15.
  • Fig. 7 shows an embodiment of the coupling device 14 as a closure part which is pivotally mounted on the housing 12 of the sensor head 5 and can be locked with a recess 19 on a pin 26 on the housing 8 of the measuring adapter 4, around the sensor head 5 on the measuring adapter 4 in a defined position keep locked.
  • the material of the windows 15 used is such that the electromagnetic radiation can fall through the windows 15 onto the detector device 17.
  • Lime-soda glass and borosilicate glass are suitable up to a wavelength range of approx. 5 ⁇ m and quartz glass up to approx. 2.5 ⁇ m.
  • silicon or sapphire up to 6.7 ⁇ m
  • Calcium fluoride (CaF 2 ), barium fluoride (BaF 2 ), germanium (Ge) or zinc selenide (ZnSe) can also be used.
  • the optical filter can also be used as a window material.
  • the windows used can be provided with an anti-reflection layer.
  • the concentration range of the gas to be detected can also be specified by the length of the radiation path 24 or the light path of the light beam in the measuring chamber 9.
  • a measuring chamber can be used in which the light path is extended by multiple reflections. For this purpose a variety of different arrangements.
  • the measuring adapter 4 is in the manner of a stopper for the sample container 2, the z. B. is a crimp bottle (see Fig. 8) formed.
  • the measuring chamber 9 is, for example, cylindrical or rectangular in cross section and is arranged in the bottle neck 21 of the crimp neck bottle.
  • the underside of the measuring chamber 9, which extends into the bottle neck 21, is closed with a cover or plate 29 reflecting the measuring radiation, in which one or more openings 33 are formed on the edge, which form a diffusion connection 33 and through which the gas to be detected can get into the measuring chamber 9 from the sample bottle 2.
  • the measuring chamber 9 is covered with a cover which is permeable to the measuring radiation, such as, for. B. an optical window 15, which is attached and sealed with a seal or with an adhesive 18.
  • a seal is placed 28 on the bottle neck 21, on which a flange of the measuring adapter 4 is placed.
  • a crimp cap 11 encompasses the bottle neck 21 and is fixed by crimping both on the lower edge of the bottle neck and on the upper side of the flange.
  • the sensor head 5 contains an approximately pot-shaped housing 12 for placement on the bottle neck 21.
  • the inside diameter of the housing wall is adapted to the crimp cap 11 in such a way that it offers a guide for the sensor head 5.
  • Inside the housing 12 is the sensor device 13 with a radiation source
  • a flexible cable 6 connects the sensor device 13 to an electronic measuring and control device 7 according to the previous example.
  • the radiation source 16 and the detector device 17 are arranged at an angle ⁇ to one another such that the measuring radiation emitted by the radiation source 16 from the reflecting plate 29 to the detector device
  • the crimp cap 11 also has an opening in the region of the recess 30.
  • a locking mechanism 32 is integrated on the inner wall of the housing, which engages to fix the sensor head 5 on the measuring adapter 4 under the edge of the bottle neck. Due to the short diffusion distances, the length of which is determined by the thickness of the plate and z. B.
  • the measuring adapter 4 can have a measuring chamber 9 with a long length, ie with a large distance between the optical disk 15 and the reflecting plate 29. By the long path of the measuring radiation 24 through the measuring chamber 9, gases with a low absorption coefficient can be monitored quantitatively.
  • the measuring adapter 9 is a simple and inexpensive to produce injection molded part with a glued-on optical window, which consists for example of silicon, which can be provided with an anti-reflection layer.
  • measuring chamber 9 in the measuring adapter 4 which is designed as a plug, is round instead of channel-shaped, no positioning device is required, since all parts are arranged symmetrically to one another (FIG. 11).
  • the measuring adapter 9 can be integrated directly into a rubber seal 34, which z. B. surrounds the circumference like a sleeve or which is an applied coating with a sealing effect, so that an additional attachment of a seal is omitted (Fig. 12).
  • the sensor device 13 shown in a bottom view in FIG. 13 contains a detector 17 and a radiation source 16.
  • a detector 17 and two radiation sources 16 and 16 ⁇ are integrated in the sensor device 13.
  • one radiation source 16 is used as the measuring source and the other radiation source 16 x is used as reference source to compensate for the aging of the measuring source at certain time intervals.
  • the radiation sources 16 and 16 ⁇ are arranged symmetrically to the detector 17, so that with both radiation sources 16 and 16 ⁇ the same light path to the detector 17 is given.
  • the sensor device 13 can also include two detectors 17 and 17 and a radiation source 16 (FIG. 15). One detector is used to measure the relevant gas concentration and the other detector is used as a reference.
  • the selection of the radiation receiver or detector devices 17 and one or more radiation sources 16 can be used to achieve selective, quantitative detection of a specific gas or also a plurality of gases.
  • the selectivity of the radiation receivers can be guaranteed by the selection of certain interference filters.
  • the interference filter can, for example, only be translucent at certain wavelengths, such as e.g. B. at 4.24 microns for carbon dioxide (C0 2 ), at 3.4 microns for hydrocarbons, at
  • the interference filters can also be arranged in front of one or more radiation sources.
  • radiation sources such.
  • B. broadband thermal emitters LEDs (light emitting diodes), diode lasers, infrared emitters or UV light emitters can be used.
  • the sensor head (5) only contains the detector device (17), for example a single detector, double detector, double detector with beam splitter, and the contact pins (40), while the radiation source (16) is located in the measuring adapter.
  • the contact surfaces (36) After touching place the sensor head on the measuring adapter (4) the contact surfaces (36) so that at least one radiation source in the measuring adapter is put into operation.
  • the radiation source (16) is integrated directly into the measuring adapter (4).
  • the measuring chamber (9) is provided with an optically transparent disc (15) and sealing compound (35).
  • the radiation source is contacted with cables (38) on the contact surfaces (36) via a solder joint (37).
  • the sample atmosphere can diffuse into the measuring chamber via diffusion connections (41).
  • Bushings (39) are provided in the measuring adapter for the cables (38).
  • the sensor head (5) contains a detector device (17). This can be, for example, a single detector, a double detector or the like.
  • the sensor head is connected to measuring electronics via a flexible cable (6).
  • the radiation source (16) is put into operation via the contact pins (40) after the sensor head (5) has been put on.
  • Fig. 17 shows an embodiment in which the measuring adapter (4) is attached to a pipe (43). This makes it possible for the sample atmosphere in the pipeline to be measured without the sensor device coming into contact with it. In addition, several measuring points can be examined in succession with one sensor head.
  • the measuring adapter can be replaced at any time since it is designed as an inexpensive single-use item.
  • Several measuring adapters (4) can be connected to a pipe (43) via a connection (42). This connection can be designed, for example, as a screw thread or snap device.
  • the sensor head from FIG. 1 can be plugged onto the measuring adapter for measuring the sample atmosphere (3). 18 shows the measurement curve which was recorded with a device according to the invention.
  • Candida parapsilosis Continuous monitoring of the growth of a yeast culture, Candida parapsilosis, was carried out with the developed measuring system. The C ⁇ 2 concentration was continuously recorded. In parallel, the same yeast culture was monitored in a second flask with a photometer for half an hour.
  • the growth can also other microorganisms such as
  • Salmonella typhymurium or E. coli bacteria are monitored. Generally with this development is the presence of microorganisms that e.g. Produce and detect carbon dioxide.
  • the device can also be used to monitor an internal atmosphere of a system from the outside. It does not matter whether the system is a closed circuit or z.
  • B. is a pipe through which a gas flows.

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Abstract

L'invention concerne un dispositif et un procédé pour l'analyse de gaz qualitative. Une analyse de gaz d'une atmosphère échantillon est réalisée au moyen d'un dispositif de détection . A cet effet, une communication de diffusion est établie entre l'atmosphère échantillon contenue dans un système à échantillon et une chambre de mesure, et l'analyse de gaz de l'atmosphère échantillon diffusée dans la chambre de mesure est réalisée au moyen du dispositif de détection. La tête de détection (5) peut être raccordée à l'adaptateur de mesure (4), et la source de rayonnement (16) et l'ensemble détecteur (17) sont fixés sur la chambre de mesure (9) selon une orientation définie. Le rayonnement de mesure (24) émis par la source de rayonnement (16) traverse la chambre de mesure (9) au moins une fois et est détecté par l'ensemble détecteur (17) après être sorti de ladite chambre de mesure (9).
EP00967587A 1999-09-15 2000-09-15 Procede et dispositif pour l'analyse de gaz quantitative Withdrawn EP1212598A2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19944260A DE19944260C2 (de) 1999-09-15 1999-09-15 Verfahren und Vorrichtung zur quantitativen Gasanalyse
DE19944260 1999-09-15
PCT/DE2000/003254 WO2001020294A2 (fr) 1999-09-15 2000-09-15 Procede et dispositif pour l'analyse de gaz quantitative

Publications (1)

Publication Number Publication Date
EP1212598A2 true EP1212598A2 (fr) 2002-06-12

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Family Applications (1)

Application Number Title Priority Date Filing Date
EP00967587A Withdrawn EP1212598A2 (fr) 1999-09-15 2000-09-15 Procede et dispositif pour l'analyse de gaz quantitative

Country Status (5)

Country Link
US (1) US6903823B1 (fr)
EP (1) EP1212598A2 (fr)
CA (1) CA2384786A1 (fr)
DE (1) DE19944260C2 (fr)
WO (1) WO2001020294A2 (fr)

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DE19944260A1 (de) 2001-04-12
CA2384786A1 (fr) 2001-03-22
US6903823B1 (en) 2005-06-07
WO2001020294A2 (fr) 2001-03-22
DE19944260C2 (de) 2001-10-04

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