Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D295/00—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms
  • C07D295/16—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms acylated on ring nitrogen atoms
  • C07D295/18—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms acylated on ring nitrogen atoms by radicals derived from carboxylic acids, or sulfur or nitrogen analogues thereof
  • C07D295/182—Radicals derived from carboxylic acids
  • C07D295/185—Radicals derived from carboxylic acids from aliphatic carboxylic acids
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K49/00—Preparations for testing in vivo
  • A61K49/04—X-ray contrast preparations
  • A61K49/0433—X-ray contrast preparations containing an organic halogenated X-ray contrast-enhancing agent
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
  • C07C237/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups
  • C07C237/28—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups having the carbon atom of at least one of the carboxamide groups bound to a carbon atom of a non-condensed six-membered aromatic ring of the carbon skeleton
  • C07C237/46—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups having the carbon atom of at least one of the carboxamide groups bound to a carbon atom of a non-condensed six-membered aromatic ring of the carbon skeleton having carbon atoms of carboxamide groups, amino groups and at least three atoms of bromine or iodine, bound to carbon atoms of the same non-condensed six-membered aromatic ring
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
  • C07C279/00—Derivatives of guanidine, i.e. compounds containing the group, the singly-bound nitrogen atoms not being part of nitro or nitroso groups
  • C07C279/04—Derivatives of guanidine, i.e. compounds containing the group, the singly-bound nitrogen atoms not being part of nitro or nitroso groups having nitrogen atoms of guanidine groups bound to acyclic carbon atoms of a carbon skeleton
  • C07C279/14—Derivatives of guanidine, i.e. compounds containing the group, the singly-bound nitrogen atoms not being part of nitro or nitroso groups having nitrogen atoms of guanidine groups bound to acyclic carbon atoms of a carbon skeleton being further substituted by carboxyl groups
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D207/00—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom
  • C07D207/02—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
  • C07D207/04—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
  • C07D207/10—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
  • C07D207/12—Oxygen or sulfur atoms
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
  • C07C2601/00—Systems containing only non-condensed rings
  • C07C2601/12—Systems containing only non-condensed rings with a six-membered ring
  • C07C2601/14—The ring being saturated

Definitions

• the present invention concerns compounds useful as contrast media for angiography, formulations thereof, and methods of using the same.
• the present invention is particularly concerned with contrast media that have anticoagulant properties.
• the present invention provides basic ionic contrast agents that have anticoagulant activity.
• a first aspect of the present invention is a compound of Formula I:
• Rl is selected from the group consisting of alkyl and alkyl substituted with hydroxy or alkoxy;
• R2, R3 and R4 are each independently selected from the group consisting of hydrogen and alkyl;
• R5 and R6 are each independently selected from the group consisting of hydrogen, alkyl and hydroxyalky, n is from 1 to 3;
• Z is -A-NHCH3, or when Rl is hydroxy or alkoxy substituted alkyl and/or when R3 is loweralkyl, Z can also be hydroxy- C2- 5 -alkylamino;
• A is:
• R7 is H or alkyl
• R8 is H, alkyl, or alkyl substituted by hydroxy or alkoxy; or together R7 and R8 form a propylene or hydroxypropylene ring; m is O or 1 ;
• R9 and RIO are each independently H or alkyl; or R9 and RIO together form C4-C8 (preferably C4) alkylene which is unsubstituted or substituted from one to three times with alkyl (e.g., methyl) or hydroxy;
• Rl 1 is amino or guanidino; o is from two to six, preferably 3 or 4; or a physiologically acceptable salt thereof.
• a second aspect of the present invention is compounds Formula II:
• Rl is selected from the group consisting of alkyl and alkyl substituted with hydroxy or alkoxy;
• R2 is selected from the group consisting of hydrogen and alkyl
• R5 and R12 are each independently selected from the group consisting of hydrogen, alkyl and hydroxyalky
• R9 and RIO are each independently H or alkyl; or R9 and RIO together form C4-C8 (preferably C5) alkylene which is unsubstituted or substituted from one to three times with alkyl (e.g., methyl) or hydroxy; Rl 1 is amino or guanidino; o is from two to six, preferably 3 or 4 or a physiologically acceptable salt thereof.
• a third aspect of the present invention is a contrast media comprising an amount of a compound of Formula I or II above effective as a contrast medium in combination with a pharmaceutically acceptable carrier.
• a fourth aspect of the present invention is the use of a compound of Formula I or II above for the preparation of a contrast media .
• a fifth aspect of the present invention is a method of visualizing an internal organ or structure of a patient which comprises administering to the patient an amount of a compound of Formula I or II above effective as a contrast medium, and then exposing said organ or structure to a diagnostic imaging treatment.
• the structure to be imaged can be a platelet or fibrin thrombus.
• Organs or structures that may be imaged by the contrast media of the present invention include blood vessels, a thrombus within a vessel, heart, brain, kidney, liver, lungs, spleen, etc. as well as portions thereof. Thus the entirety of an organ or structure need not be imaged, but only the portion thereof of diagnostic interest. Diagnostic imaging treatments used to carry out the method of the present invention may be of any type, including magnetic resonance imaging and CT, but typically are X-ray diagnostic imaging treatments.
• Alkyl as used herein is C 1-4 loweralkyl; alkoxy as used herein is C1-C4 loweralkoxy, and more preferably Cl-2 loweralkoxy.
• the lower alkyl residue Rl which where appropriate can be substituted singly or multiply, may contain from 1 to 4 carbon atoms, preferably 1 to 2 carbon atoms, in the alkyl residue. Suitable substituents include hydroxy, preferably 1-2 groups, or alkoxy of 1-2 carbon atoms, preferably one group. Preferred lower alkyl residues Rl have 1-2 carbon atoms and may be substituted by hydroxy or alkoxy, such as, e.g., methyl, ethyl, methoxymethyl, hydroxymethyl and the like.
• Suitable lower alkyl residues for R2, R3 and R4 have 1-4 carbon atoms, preferably 1-2 carbon atoms, for instance methyl, ethyl, propyl, isopropyl and the like.
• Suitable lower alkyl residues for R5 have 1-4 carbon atoms and especially have 1-2 carbon atoms, for instance, methyl or ethyl.
• This alkyl residue may optionally be substituted by hydroxy, preferably 1-2 groups; n preferably has a value of l.
• Z is straight-chain or branched hydroxy lower alkylamino
• the alkyl residue may contain 2-5 carbon atoms, and, e.g., 1-3 hydroxy substituents.
• the alkyl residue preferably contains 2-5 carbon atoms; if Z is branched, the alkyl residue preferably contains 3-5 carbon atoms.
• the hydroxy groups in Z may be present as primary or secondary hydroxy groups.
• Suitable Z residues include, e.g., 2-hydroxypropylamino, 3-hydroxypropylamino, 2-hydroxy-l, 1- dimethylethylamino, 3 -hydroxy- 1, 1-dimethylpropylamino and, preferably, 2- hy droxy ethy lamino .
• Suitable bridging amino acid residues A include those derived from an amino carboxylic acid of arbitrary configuration. Included are those residues which are equivalents of, e.g., the preferred amino acid residue of the formula
• R7 is hydrogen or lower alkyl of 1-4 carbon atoms, preferably 1- 2 carbon atoms
• R8 is hydrogen or lower alkyl of 1-4 carbon atoms which also may be straightchain or branched and which may be substituted by hydroxy, preferably 1-2 groups or lower alkoxy of 1-2 carbon atoms, preferably 1-2 groups, and wherein R7 and R8 together may form a propylene or hydroxypropylene group.
• suitable aminocarboxylic acids include glycine, alanine, valine, serine, O- methylserine, proline, hydroxyproline, leucine, isoleucine, sarcosine, beta-alanine and the like.
• alpha -amino-carboxylic acids in which R7 is hydrogen or methyl and R8 is hydrogen or lower alkyl of 1-2 carbon atoms which may be hydroxy- or methoxy-substituted.
• Still additional examples of compounds of the present invention include the following:
• salts of the active compound may have the general formula R ⁇ X " , where R + is the active compound as described above and X- is Cl “ , HCOO “ , H 2 citrate “ , CH 3 COO, NH 2 CH 2 COO ⁇ HCO 3 " , H 2 PO 4 ⁇ or HSO 3 ⁇
• the new compounds of this invention are useful as contrast media, and are especially suited for use in urography, angiography, bronchography, etc., for visualization of body cavities and also for computer tomography.
• the preparation and administration of new contrast media from the compounds of this invention is in accordance with known techniques.
• the contrast compound can be combined with conventional galenic adjuvants to form a composition suitable for the desired method of application.
• the compounds of this invention can be employed in mixture with conventional excipients, i.e., pharmaceutically acceptable organic or inorganic carrier substances suitable for parenteral or enteral application which do not deleteriously react with the active compounds.
• Suitable pharmaceutically acceptable carriers include but are not limited to water, salt solutions, alcohols, vegetable oils, polyethylene glycols, gelatin, lactose, amylose, magnesium stearate, viscous paraffm, perfume oil, fatty acid monoglycerides and diglycerides, pentaerythritol fatty acid esters, hydroxy methylcellulose, polyvinyl pyrrolidone, talc, etc.
• solutions preferably oily or aqueous solutions, as well as suspensions or emulsions.
• Ampoules are convenient unit dosages.
• tablets or dragees having the talc and or a carbohydrate carrier or binder or the like, the carrier preferably being lactose and/or corn starch and/or potato starch.
• a syrup or the like can be used wherein a sweetened vehicle is employed.
• Sustained release compositions can also be formulated wherein the active compound is protected with differentially degradable coatings, e.g., by microencapsulation, multiple coatings, etc.
• concentration of the new contrast media of this invention in aqueous media depends on the particular diagnostic method involved.
• concentrations and doses of the compounds of this invention e.g., for X-ray diagnoses, are concentrations of 50-400 mg of iodine per ml and doses of 10-500 ml. Concentrations of 100-350 mg of iodine per ml are especially preferred.
• DMAP means dimethylaminopyridine
• DMAc means dimethylacetamide
• BOC means t-butylcarbamate
• DMF means dimethylformamide
• nMM means N-methylmorpholine
• nMP means N-methylpiperidine
• 4MP means 4- methylpiperidine
• iBCF means isobutylchloroformate
• DIEA means diethylamine
• THF means tetrahydrof ⁇ ran
• F oc means 9-fluorenylrnethoxycarbonyl
• Lys means lysine
• AcOH means acetic acid
• AcOEt means ethyl acetate
• NMR means Nuclear
• TLC Magnetic Resonance Spectroscopy
• N Normal
• h hour
• min. minutes
• RT room temperature
• temperatures are given in degrees centigrade.
• step 2 FmocLys ⁇ oc) (1 Equiv) and nMM (1 Equiv) in THF is cooled to O°C and iBCF (1 Equiv) is added.
• step 2 after 15 minutes 4-methylpiperidine (1 Equiv) is added and the mixture is maintained at O°C for 10 minutes and then allowed to warm to room temperature for 40 minutes.
• step 3 the reaction mixture is concentrated under reduced pressure and extracted with EtOAc; washed (0.5 N KHCO 3 , 0.5 N HC1) and evaporated. TLC is performed to verify reaction components.
• step 1 the crude FmocLys(Boc)4MP is dissolved in THF and DIEA (9/1, v/v) and allowed to stand at RT for 2 h.
• step 2 the reaction mixture is concentrated under reduced pressure and triturated with ether/hexane (4/1 , v/v). TLC is performed as well as NMR and Mass Spectrometry.
• Examples 2-5 illustrate the preparation of Compound 1 above, which is illustrative of Formula I above.
• step 1 the product of Example 2 is placed in a 3-necked round bottom flask equipped with a mechanical stirrer, a dropping funnel and a condenser.
• step 2 thionyl chloride (16 Equiv.) is rapidly added to the flask via the dropping funnel. The solution is heated to 80-85°C with an oil bath for three hours. In step 3, check for product by thin layer chromatography (100% ethyl acetate).
• step 4 the condenser is replaced with a distilling arm. Excess thionyl chloride is removed by distillation under house vacuum (oil bath temperature 50°C).
• step 5 THF is added and distilled. The addition/distillation is repeated twice.
• step 6 the product (AcHexCl) is extracted as an oil with ethyl acetate, washing with saturated NaHCO 3 and NaCl. The product is dried over Na 2 SO 4 , filtered and concentrated under reduced pressure.
• step 1 the product of Example 3 (AcHexCl) and sodium carbonate (1 Equiv) is placed in a round bottom flask equipped with a magnetic stir bar. Anhydrous DMAc is added and stirred under nitrogen for five minutes.
• step 2 the product of Example 1 is added (41% w/v in DMAc, 1.5 Equiv.) via a syringe to the flask. The contents are stirred overnight under nitrogen at room temperature.
• step 3 the reaction mixture is filtered through celite and the DMAc is removed by distillation under high vacuum.
• step 4 methanol is added to dissolve the oil and then water is added.
• the reaction solution is passed through an IRA-120H resin and the eluent collected.
• the resin is washed with water/methanol (1 :1, v/v) and the eluent washes combined.
• the solution is then used for the acidic deprotection of the compound.
• Example 4 The product of Example 4 is acidified to pH 1.0 in methanol/water solution with concentrated H 2 SO 4 .
• the mixture is then heated with an oil bath to reflux for 2 hours and the product concentrated.
• the solution is subjected to TLC with EtOAc/Methanol/AcOH, 10:5:1 v/v/v.
• the filtrate is then placed on an exchange column (Amberlite IRA-68 weakly basic anion exchange resin on top of IRA-458 strongly basic anion exchange resin) and the filtrate collected and the column washed with deionized water.
• the filtrate is concentrated under reduced pressure and then under high vacuum with the flask warmed at 50°C in a water bath overnight.
• Lys(Boc)4MP prepared as described in Example 1 (1.5 Equiv.) is added to a sodium carbonate solution of the acid chloride under nitrogen and stirred overnight at room temperature. The reaction mixture is filtered, concentrated, and extracted to yield the protected amide intermediate illustrated below.
• Compound 1 and Compound 10 as described above are prepared as a formulation as follows: 320 mg Iodine/ml (420 mM of compound 1 and 845 mM of compound 10) is pH adjusted to 7.4 to 8.8 with NaOH or HC1 in an aqueous solution consisting of 10 mM tromethane (Tris), 0.10 mg/ml edetate calcium disodium, 19 mM NaCl, and 0.3 mM Calcium chloride, dihydrate.
• Tris 10 mM tromethane
• edetate calcium disodium 19 mM NaCl
• 0.3 mM Calcium chloride dihydrate.
• the usual dose for left coronary is 2-14 mL (typically 8 mL) of the formulation described above, and the usual dose for right coronary arteriography is 1 to 10 mL (typically 5 mL) of the formulation described above.
• the doses may be repeated as necessary. Doses up to a total of 150 mL are suitable.
• the usual dose in a single injection is 35-45 mL (typically 45 mL) and repeated as necessary.
• the total dose for combined selective coronary arteriography and left ventriculography should not exceed 250 mL.
• the usual single adult dose for aorto-iliac runoff studies is 20 to 80 mL (typically 45 mL).
• the usual single adult dose for the common iliac, the external iliac and the femoral arteries is 10-50 mL (typically 30 mL). These doses may be repeated as necessary.
• the usual single adult dose is 20 mL (range 15-30 mL) repeated as necessary.
• the total procedural dose should not exceed 250 mL.
• the usual dose for injections into the aort is 25 to 50 mL; the usual dose for injection into the celiac artery is 40 mL; the usual dose for injection into the superior mesenteric artery is 20 to 40 mL; the usual dose for injection into the inferior mesenteric artery is 8 to 15 mL. These doses may be repeated as necessary. The total dose should not exceed 250 mL.

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