EP1121447A2 - Systemes et procedes pour moduler les caracteristiques stomatiques de vegetaux - Google Patents

Systemes et procedes pour moduler les caracteristiques stomatiques de vegetaux

Info

Publication number
EP1121447A2
EP1121447A2 EP99953777A EP99953777A EP1121447A2 EP 1121447 A2 EP1121447 A2 EP 1121447A2 EP 99953777 A EP99953777 A EP 99953777A EP 99953777 A EP99953777 A EP 99953777A EP 1121447 A2 EP1121447 A2 EP 1121447A2
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
plant
plants
acid molecule
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP99953777A
Other languages
German (de)
English (en)
Inventor
Dieter Berger
Thomas Altmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Bayer Bioscience GmbH
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Planttec Biotechnologie GmbH Forschung and Entwicklung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV, Planttec Biotechnologie GmbH Forschung and Entwicklung filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority to EP99953777A priority Critical patent/EP1121447A2/fr
Publication of EP1121447A2 publication Critical patent/EP1121447A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Definitions

  • the present invention relates to recombinant DNA molecules comprising nucleic acid molecules encoding subtilisin-like serine proteases that are involved in the regulation of stomatal density in plants; wherein said nucleic acid molecules could be operably linked to regulatory elements allowing the expression of the nucleic acid molecules in plants.
  • the present invention also provides vectors comprising said recombinant DNA molecules as well as plant cells, plant tissues and plants transformed therewith.
  • the present invention further relates to the use of the aforementioned recombinant DNA molecules and vectors in plant cell and tissue culture, plant breeding and/or agriculture, in particular for the production of plants with improved traits.
  • the invention relates to a recombinant DNA molecule comprising
  • nucleic acid molecule encoding a subtilisin-like serine protease or encoding a biologically active fragment of such a protein, selected from the group consisting of
  • nucleic acid molecules encoding a protein the amino acid sequence of which is at least 65% identical to the amino acid sequence encoded by a nucleic acid molecule of any one of (a) to (c); (f) nucleic acid molecules, the nucleotide sequence of which is degenerate as a result of the genetic code to a nucleotide sequence of a nucleic acid molecule as defined in any one of (a) to (e); or
  • HVSGI SEQ ID NO: 47
  • FTV-SGT SEQ ID NO: 48
  • promoters from the two SDD1 homologs from Solanum toberosum described in Example 6 can be isolated by conventional means.
  • DNA molecules encoding an antisense RNA which is complementary to transcripts of a DNA molecule of the invention are also the subject matter of the present invention, as well as these antisense molecules.
  • complementarity does not signify that the encoded RNA has to be 100% complementary.
  • a low degree of complementarity is sufficient, as long as it is high enough in order to inhibit the expression of a protein of the invention upon expression in plant cells.
  • the transcribed RNA is preferably at least 90% and most preferably at least 95% complementary to the transcript of the nucleic acid molecule of the invention.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne l'utilisation de molécules d'acides nucléiques codant pour les sérine protéases de type de subtilisine ainsi que la modulation des gènes correspondants visant la production de plantes transgéniques possédant des caractéristiques stomatiques modifiées. L'invention concerne notamment des molécules d'ADN recombinants comprenant ces molécules d'acides nucléiques et leurs compléments; cette (ces) molécule(s) d'acides nucléiques est (sont) liée(s) de manière fonctionnelle aux éléments régulateurs de manière à permettre l'expression de cette (ces) molécule(s) d'acides nucléiques. L'invention concerne également des cellules végétales, des tissus de plantes et des plantes transformées au moyen de cette (ces) molécule(s) d'acides nucléiques; elle concerne en outre l'utilisation de ces molécules d'acides nucléiques, des molécules et des vecteurs d'ADN recombinants avec des cellules végétales et des cultures de tissus, dans la sélection de plantes et/ou dans des méthodes d'agriculture, notamment pour la production de plantes à phénotype amélioré.
EP99953777A 1998-10-12 1999-10-12 Systemes et procedes pour moduler les caracteristiques stomatiques de vegetaux Withdrawn EP1121447A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP99953777A EP1121447A2 (fr) 1998-10-12 1999-10-12 Systemes et procedes pour moduler les caracteristiques stomatiques de vegetaux

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP98119244 1998-10-12
EP98119244 1998-10-12
PCT/EP1999/007633 WO2000022144A2 (fr) 1998-10-12 1999-10-12 Systemes et procedes pour moduler les caracteristiques stomatiques de vegetaux
EP99953777A EP1121447A2 (fr) 1998-10-12 1999-10-12 Systemes et procedes pour moduler les caracteristiques stomatiques de vegetaux

Publications (1)

Publication Number Publication Date
EP1121447A2 true EP1121447A2 (fr) 2001-08-08

Family

ID=8232779

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99953777A Withdrawn EP1121447A2 (fr) 1998-10-12 1999-10-12 Systemes et procedes pour moduler les caracteristiques stomatiques de vegetaux

Country Status (8)

Country Link
EP (1) EP1121447A2 (fr)
JP (1) JP2002527070A (fr)
CN (1) CN1323347A (fr)
AR (1) AR020775A1 (fr)
AU (1) AU1035000A (fr)
BR (1) BR9914424A (fr)
CA (1) CA2346259A1 (fr)
WO (1) WO2000022144A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002083911A1 (fr) * 2001-04-12 2002-10-24 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Production de plantes a tolerance amelioree au stress de secheresse ou a transpiration augmentee
WO2007086402A1 (fr) * 2006-01-25 2007-08-02 Osaka University Facteur de régulation stomatique végétal
US9057074B2 (en) 2008-03-25 2015-06-16 Biogemma Pedicel specific promoter
JP5825574B2 (ja) * 2009-12-07 2015-12-02 国立大学法人京都大学 気孔増加剤、ポリペプチド、植物における気孔の数および／または密度の増加方法ならびに植物の収量の増加方法
CA2875236A1 (fr) * 2012-06-22 2013-12-27 Julian I. Schroeder Compositions et procedes pour medier le developpement des stomates chez les plantes en reponse au dioxyde de carbone et applications de manipulation de la tolerance a la secheresse chez les plantes
CN110656114B (zh) * 2019-10-18 2022-07-01 云南中烟工业有限责任公司 一种烟草色素合成相关的基因及其应用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0022144A3 *

Also Published As

Publication number Publication date
CN1323347A (zh) 2001-11-21
JP2002527070A (ja) 2002-08-27
AU1035000A (en) 2000-05-01
WO2000022144A3 (fr) 2000-11-23
BR9914424A (pt) 2001-06-26
WO2000022144A2 (fr) 2000-04-20
AR020775A1 (es) 2002-05-29
CA2346259A1 (fr) 2000-04-20

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