Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
  • G01N15/10—Investigating individual particles
  • G01N15/14—Optical investigation techniques, e.g. flow cytometry
  • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
  • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
  • G01N35/00069—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
  • G01N15/10—Investigating individual particles
  • G01N15/14—Optical investigation techniques, e.g. flow cytometry
  • G01N15/1429—Signal processing
  • G01N15/1433—Signal processing using image recognition
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
  • G01N15/10—Investigating individual particles
  • G01N15/14—Optical investigation techniques, e.g. flow cytometry
  • G01N15/1434—Optical arrangements
  • G01N2015/1438—Using two lasers in succession
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
  • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
  • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
  • G01N21/64—Fluorescence; Phosphorescence
  • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
  • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
  • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
  • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
  • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
  • G01N33/54326—Magnetic particles

Definitions

• the apparatus comprises a tracking device for tracking a position of the object to be identified in two dimensions and an identification device for identification of the tracked object.
• the objects to be identified may be stained so as to distinguish them from other objects by a difference in transmissivity at a specific range of • wavelengths.
• the object identification device comprises a coherent light source illuminating an area of the specimen and a detection device with a plurality of circular coaxial sections which are detecting the intensity distribution of a Fourier transform of light transmitted through the objects of the specimen.
• the positions of the detected target objects may subsequently be retrieved from the memory device, and the objects may be subjected to a thorough analysis in order to establish their identity.
• the large focus area which may be provided by an embodiment of the present apparatus, enables rapid scanning of even very large specimens.
• a disc is used as the solid support holding the specimen, there exist, in principle, only few limitations to the disc size.
• a disc diameter of approximately 120 mm which is equal to the diameter of a CD, is utilised.
• the effective area of a CD size disc is at least twenty times larger than the typical solid support area used in prior art apparatuses.
• suitable mechanical and optical parts for the present apparatus may be found readily available and at a very low cost due to the wide spread use of CD based media.
• the disc may be manufactured in a transparent material, such as polycarbonate, glass, etc.
• the dimensions of the aperture are, preferably, adapted to the dimensions of the target objects to be detected and located, in such a manner that the width of the aperture is approximately equal to the dimensions of the target objects and the length is approximately equal to 3-5 times the target objects' dimensions, as projected on the specimen.
• the dimensions of the aperture should be adapted to any magnification/demagnification provided in the optical path between the specimen and the aperture.
• the laser spot irradiating the specimen is, preferably, provided with a diameter approximately equal to the aperture length.
• a fluorescent marker should, preferably, posses a large quantum efficiency at one hand, and at the other hand posses a large difference between the optimum wavelength of excitation light and the optimum wavelength of emission light.
• Fluorescein or Fluorescein derivatives may be used as a fluorescent marker(s), since these substances are well known, readily available and possess large quantum efficiencies.
• optimum wavelengths for excitation light and for emission light are located at 505 nm and 516 nm, respectively, for Fluorescein, which makes it possible to excite this substance by a preferred light source, a 488 nm argon-ion gas laser.
• Deflecting means which form part of the scanning means, and comprise a stepper motor (not shown), are further provided so the disc 13 with the specimen 14 may be displaced along a radius of the disc and its circular movement. Thereby providing a scan of the entire surface area of the disc 13 with the specimen 14 by the laser spot.
• a non-linear curve comprising a number of concentric partly overlapping circular curves is formed on the disc 13 surface by the laser spot.

Landscapes

• Chemical & Material Sciences (AREA)
• Pathology (AREA)
• Immunology (AREA)
• Life Sciences & Earth Sciences (AREA)
• Analytical Chemistry (AREA)
• Biochemistry (AREA)
• General Health & Medical Sciences (AREA)
• General Physics & Mathematics (AREA)
• Health & Medical Sciences (AREA)
• Physics & Mathematics (AREA)
• Dispersion Chemistry (AREA)
• Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
• Analysing Materials By The Use Of Radiation (AREA)
• Vehicle Body Suspensions (AREA)
• Automatic Control Of Machine Tools (AREA)
• Control Of Conveyors (AREA)
• Apparatus Associated With Microorganisms And Enzymes (AREA)
• Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
• Image Analysis (AREA)
• Measurement Of Velocity Or Position Using Acoustic Or Ultrasonic Waves (AREA)

Priority Applications (1)

Applications Claiming Priority (3)

Publications (2)

Family

ID=8102629

Family Applications (1)

Country Status (8)

Families Citing this family (9)

Family Cites Families (23)

• 1999
  • 1999-09-30 AT AT99945961T patent/ATE310239T1/de active
  • 1999-09-30 WO PCT/DK1999/000515 patent/WO2000020838A1/en active IP Right Grant
  • 1999-09-30 ES ES99945961T patent/ES2253910T3/es not_active Expired - Lifetime
  • 1999-09-30 DE DE69928423T patent/DE69928423T2/de not_active Expired - Lifetime
  • 1999-09-30 DK DK99945961T patent/DK1117983T3/da active
  • 1999-09-30 US US09/806,457 patent/US8362446B1/en not_active Expired - Fee Related
  • 1999-09-30 AU AU58504/99A patent/AU5850499A/en not_active Abandoned
  • 1999-09-30 EP EP99945961A patent/EP1117983B1/de not_active Expired - Lifetime

Non-Patent Citations (1)

Also Published As

Similar Documents

Legal Events