EP1104479A2 - Verfahren zur gewinnung von proteinen aus der interstitiellen flüssigkeit von pflanzen - Google Patents
Verfahren zur gewinnung von proteinen aus der interstitiellen flüssigkeit von pflanzenInfo
- Publication number
- EP1104479A2 EP1104479A2 EP99939728A EP99939728A EP1104479A2 EP 1104479 A2 EP1104479 A2 EP 1104479A2 EP 99939728 A EP99939728 A EP 99939728A EP 99939728 A EP99939728 A EP 99939728A EP 1104479 A2 EP1104479 A2 EP 1104479A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- protein
- plant
- tissue
- interest
- buffer solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01045—Glucosylceramidase (3.2.1.45), i.e. beta-glucocerebrosidase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/57—IFN-gamma
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
- C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2422—Alpha-amylase (3.2.1.1.) from plant source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01022—Alpha-galactosidase (3.2.1.22)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/13—Immunoglobulins specific features characterized by their source of isolation or production isolated from plants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Pharmacology & Pharmacy (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Fertilizers (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13298998A | 1998-08-11 | 1998-08-11 | |
US132989 | 1998-08-11 | ||
PCT/US1999/018161 WO2000009725A2 (en) | 1998-08-11 | 1999-08-11 | Method for recovering proteins from the interstitial fluid of plant tissues |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1104479A2 true EP1104479A2 (de) | 2001-06-06 |
EP1104479B1 EP1104479B1 (de) | 2006-10-18 |
Family
ID=22456515
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP99939728A Expired - Lifetime EP1104479B1 (de) | 1998-08-11 | 1999-08-11 | Verfahren zur gewinnung von proteinen aus der interstitiellen flüssigkeit von pflanzen |
Country Status (14)
Country | Link |
---|---|
US (5) | US6284875B1 (de) |
EP (1) | EP1104479B1 (de) |
JP (1) | JP2002522559A (de) |
KR (1) | KR20010072372A (de) |
AT (1) | ATE342994T1 (de) |
AU (1) | AU759813B2 (de) |
BR (1) | BR9912875A (de) |
CA (1) | CA2349852A1 (de) |
DE (1) | DE69933677T2 (de) |
IL (1) | IL141312A0 (de) |
MX (1) | MXPA01001445A (de) |
NZ (1) | NZ510358A (de) |
WO (1) | WO2000009725A2 (de) |
ZA (1) | ZA200101100B (de) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9056894B2 (en) | 2010-10-27 | 2015-06-16 | Philip Morris Products S.A. | Methods for capturing virus like particles from plants using expanded bed chromatogrpahy |
Families Citing this family (75)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR9912875A (pt) * | 1998-08-11 | 2002-02-13 | Large Scale Biology Corp | Método para a recuperação de proteìnas a partir do fluido intersticial de tecidos vegetais |
IL147343A0 (en) * | 1999-07-21 | 2002-08-14 | Large Scale Biology Corp | Method for enhancing ran or protein production using non-native 5' untranslated sequences in recombinant viral nucleic acids |
EP1349946B1 (de) | 2000-08-25 | 2011-01-26 | BASF Plant Science GmbH | Polynukleotide, die für prenylproteasen aus pflanzen kodieren |
US7629309B2 (en) * | 2002-05-29 | 2009-12-08 | Zystor Therapeutics, Inc. | Targeted therapeutic proteins |
ES2300439T3 (es) * | 2001-04-30 | 2008-06-16 | Zystor Therapeutics , Inc. | Reconocimiento subcelular de proteinas terapeuticas. |
US7560424B2 (en) | 2001-04-30 | 2009-07-14 | Zystor Therapeutics, Inc. | Targeted therapeutic proteins |
US20040005309A1 (en) * | 2002-05-29 | 2004-01-08 | Symbiontics, Inc. | Targeted therapeutic proteins |
US20030072761A1 (en) * | 2001-10-16 | 2003-04-17 | Lebowitz Jonathan | Methods and compositions for targeting proteins across the blood brain barrier |
US6817970B2 (en) * | 2002-06-17 | 2004-11-16 | Large Scale Biology Corporation | Centrifuge for extracting interstitial fluid |
US7783159B2 (en) * | 2002-06-24 | 2010-08-24 | Lg Electronics Inc. | Recording medium having data structure for managing reproduction of multiple reproduction path video data for at least a segment of a title recorded thereon and recording and reproducing methods and apparatuses |
KR20040000290A (ko) * | 2002-06-24 | 2004-01-03 | 엘지전자 주식회사 | 고밀도 광디스크의 멀티 경로 데이터 스트림 관리방법 |
EP1629090B1 (de) * | 2002-11-06 | 2014-03-05 | iBio, Inc. | Expression von fremdsequenzen in pflanzen unter verwendung eines trans-aktivierungssystems |
US7692063B2 (en) * | 2002-11-12 | 2010-04-06 | Ibio, Inc. | Production of foreign nucleic acids and polypeptides in sprout systems |
US7683238B2 (en) * | 2002-11-12 | 2010-03-23 | iBio, Inc. and Fraunhofer USA, Inc. | Production of pharmaceutically active proteins in sprouted seedlings |
ES2531125T3 (es) * | 2003-02-03 | 2015-03-10 | Ibio Inc | Sistema para la expresión de genes en plantas |
US7809775B2 (en) * | 2003-02-27 | 2010-10-05 | Lg Electronics, Inc. | Recording medium having data structure for managing playback control recorded thereon and recording and reproducing methods and apparatuses |
EP1604356A4 (de) * | 2003-02-28 | 2009-12-16 | Lg Electronics Inc | Aufzeichnungsmedium mit datenstruktur zur verwaltung der random/shuffle-wiedergabe von darauf aufgezeichneten videodaten sowie verfahren und vorrichtungen zur aufzeichnung und wiedergabe |
CN103074316B (zh) | 2003-05-22 | 2015-10-21 | 美国弗劳恩霍夫股份有限公司 | 用于表达、传递及纯化目标多肽的重组载体分子 |
US8158379B2 (en) * | 2003-10-28 | 2012-04-17 | Allosource | Methods for determining microbial contamination of allograft products |
AU2005212435B2 (en) * | 2004-02-10 | 2010-09-09 | Biomarin Pharmaceutical Inc. | Acid alpha-glucosidase and fragments thereof |
CA2555230A1 (en) | 2004-02-20 | 2005-09-09 | Fraunhofer Usa Inc. | Systems and methods for clonal expression in plants |
US20050220901A1 (en) * | 2004-03-22 | 2005-10-06 | Huttenbauer Samuel Jr | Methods of pharmaceutical separation from plants |
CA2570566A1 (en) | 2004-06-25 | 2006-01-12 | Altor Bioscience Corporation | Production of tissue factor in plants |
US20060288449A1 (en) * | 2004-10-12 | 2006-12-21 | Garger Stephen J | Process for purifying target compounds from plant sources using ceramic filtration |
WO2006130529A1 (en) * | 2005-05-31 | 2006-12-07 | Pierce Biotechnology, Inc. | Extraction of cellular components with fatty acid derivatives |
CN101378781B (zh) * | 2005-08-03 | 2013-08-07 | 美国弗劳恩霍夫股份有限公司 | 制造免疫球蛋白的组合物和方法 |
EP1984388B1 (de) * | 2006-02-13 | 2016-07-06 | iBio, Inc. | Hpv-antigene, impfstoffzusammensetzungen und zugehörige verfahren |
US8124103B2 (en) * | 2006-02-13 | 2012-02-28 | Fraunhofer Usa, Inc | Influenza antigens, vaccine compositions, and related methods |
US8277816B2 (en) * | 2006-02-13 | 2012-10-02 | Fraunhofer Usa, Inc. | Bacillus anthracis antigens, vaccine compositions, and related methods |
TW200745337A (en) * | 2006-02-24 | 2007-12-16 | Aminotech As | Modified process |
JP2010509344A (ja) * | 2006-11-13 | 2010-03-25 | ザイストール セラピューティクス, インコーポレイテッド | ポンペ病を治療するための方法 |
EP2152301A4 (de) * | 2007-04-28 | 2010-07-28 | Fraunhofer Usa Inc | Trypanosomaantigene, impfstoffzusammensetzungen und zugehörige verfahren |
US9321806B2 (en) * | 2007-05-16 | 2016-04-26 | University Of Maryland, College Park | Methods for recovery of leaf proteins |
US8404252B2 (en) * | 2007-07-11 | 2013-03-26 | Fraunhofer Usa, Inc. | Yersinia pestis antigens, vaccine compositions, and related methods |
CA2615372A1 (en) | 2007-07-13 | 2009-01-13 | Marc-Andre D'aoust | Influenza virus-like particles (vlps) comprising hemagglutinin |
US20110059130A1 (en) * | 2007-08-20 | 2011-03-10 | Fraunhofer Usa, Inc. | Prophylactic and therapeutic influenza vaccines, antigens, compositions and methods |
DK2610345T3 (en) | 2007-11-27 | 2015-12-07 | Medicago Inc | RECOMBINANT INFLUENZA VIRUS SIMULAR PARTICULARS (VLPS) MADE IN TRANSGENE PLANTS THAT EXPRESS HEMAGGLUTININ |
EP2113514A1 (de) | 2008-05-03 | 2009-11-04 | Westfälische Wilhelms-Universität Münster | Verfahren zur Erzeugung von hypoallergenen Glykoproteinen in mutierten oder transgenen Pflanzen oder Pflanzenzellen sowie mutierte oder transgene Pflanzen und Pflanzenzellen zur Erzeugung hypoallergener Glykoproteine |
BRPI0912225A2 (pt) * | 2008-05-07 | 2018-03-20 | Zystor Therapeutics, Inc. | peptídeos de direcionamento lisossomais e usos dos mesmos |
ES2545607T3 (es) | 2008-07-08 | 2015-09-14 | Medicago Inc. | Antígenos de la gripe recombinantes solubles |
WO2010037046A1 (en) | 2008-09-28 | 2010-04-01 | Fraunhofer Usa, Inc. | Humanized neuraminidase antibody and methods of use thereof |
EP3075386B1 (de) | 2009-06-17 | 2019-10-16 | BioMarin Pharmaceutical Inc. | Formulierungen für lysosomale enzyme |
MX349924B (es) | 2009-06-24 | 2017-08-21 | Medicago Inc * | Partículas similares al virus de la influenza quiméricas que comprenden hemaglutinina. |
IN2012DN02637A (de) * | 2009-09-22 | 2015-09-04 | Medicago Inc | |
CA2776144C (en) | 2009-09-29 | 2020-10-27 | Fraunhofer Usa, Inc. | Influenza hemagglutinin antibodies, compositions, and related methods |
CN103180447A (zh) | 2010-07-19 | 2013-06-26 | 加州大学评议会 | 异源蛋白质的植物基制备 |
US9155321B2 (en) | 2010-08-11 | 2015-10-13 | R.J. Reynolds Tobacco Company | Meltable smokeless tobacco composition |
US9220295B2 (en) | 2010-12-01 | 2015-12-29 | R.J. Reynolds Tobacco Company | Tobacco separation process for extracting tobacco-derived materials, and associated extraction systems |
US9204667B2 (en) | 2010-12-01 | 2015-12-08 | R.J. Reynolds Tobacco Company | Smokeless tobacco pastille and injection molding process for forming smokeless tobacco products |
US9775376B2 (en) | 2010-12-01 | 2017-10-03 | R.J. Reynolds Tobacco Company | Smokeless tobacco pastille and moulding process for forming smokeless tobacco products |
TWI620816B (zh) | 2011-03-23 | 2018-04-11 | 苜蓿股份有限公司 | 植物衍生蛋白回收方法 |
US20130118512A1 (en) | 2011-11-16 | 2013-05-16 | R.J. Reynolds Tobacco Company | Smokeless tobacco products with starch component |
US20130125904A1 (en) | 2011-11-18 | 2013-05-23 | R.J. Reynolds Tobacco Company | Smokeless tobacco product comprising pectin component |
US9546219B2 (en) | 2012-02-08 | 2017-01-17 | North Carolina State University | Treatment of allergic diseases with recombinant antibodies |
US20130206150A1 (en) | 2012-02-10 | 2013-08-15 | R.J. Reynolds Tobacco Company | Multi-layer smokeless tobacco composition |
US9339058B2 (en) | 2012-04-19 | 2016-05-17 | R. J. Reynolds Tobacco Company | Method for producing microcrystalline cellulose from tobacco and related tobacco product |
US9617297B2 (en) * | 2012-10-11 | 2017-04-11 | The Regents Of The University Of California | Apoplast wash fluid recovery for improved recombinant endoglucanase extraction in tabacco leaves |
US10357054B2 (en) | 2013-10-16 | 2019-07-23 | R.J. Reynolds Tobacco Company | Smokeless tobacco pastille |
US9375033B2 (en) | 2014-02-14 | 2016-06-28 | R.J. Reynolds Tobacco Company | Tobacco-containing gel composition |
CN104195170B (zh) * | 2014-09-16 | 2016-04-13 | 云南省农业科学院生物技术与种质资源研究所 | 一种水稻真空渗透遗传转化方法 |
US10869497B2 (en) | 2015-09-08 | 2020-12-22 | R.J. Reynolds Tobacco Company | High-pressure cold pasteurization of tobacco material |
US11612183B2 (en) | 2015-12-10 | 2023-03-28 | R.J. Reynolds Tobacco Company | Protein-enriched tobacco composition |
CA3041717A1 (en) | 2016-11-09 | 2018-05-17 | North Carolina State University | Treatment of allergic diseases with chimeric protein |
US10757964B2 (en) | 2017-07-20 | 2020-09-01 | R.J. Reynolds Tobacco Company | Purification of tobacco-derived protein compositions |
BE1025528B1 (fr) * | 2017-09-06 | 2019-04-05 | Universite De Liege Établissement Public De La Communauté Française De Belgique | Dispositif non-destructif d'echantillon pour extraction de marqueurs |
US20190307082A1 (en) | 2018-04-05 | 2019-10-10 | R.J. Reynolds Tobacco Company | Oriental tobacco production methods |
EP3801056A4 (de) | 2018-05-30 | 2022-03-16 | LeafPro, LLC. | Gereinigte proteinkonzentrate von süssen kartoffelblättern und verfahren zur rückgewinnung |
US11312753B2 (en) * | 2018-09-25 | 2022-04-26 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and systems to produce lignin-modifying enzymes and uses thereof |
US11523623B2 (en) | 2019-01-18 | 2022-12-13 | R.J. Reynolds Tobacco Company | Plant-derived protein purification |
EP3808854A1 (de) * | 2019-10-17 | 2021-04-21 | eleva GmbH | Verfahren zur gewinnung von material von pflanzenzelloberflächen |
WO2021116855A1 (en) | 2019-12-09 | 2021-06-17 | Nicoventures Trading Limited | Oral compositions and methods of manufacture |
CN111004766B (zh) * | 2019-12-30 | 2022-03-18 | 浙江大学 | 一种从植物叶片中提取纯化内质网及其蛋白的有效方法 |
CN116194461A (zh) * | 2020-08-05 | 2023-05-30 | 电化株式会社 | 目标蛋白质的回收方法及制造方法 |
EP4326097A1 (de) | 2021-04-22 | 2024-02-28 | Nicoventures Trading Limited | Orale zusammensetzungen und herstellungsverfahren |
US20220354785A1 (en) | 2021-04-22 | 2022-11-10 | Nicoventures Trading Limited | Oral lozenge products |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3910822A (en) * | 1974-03-14 | 1975-10-07 | Us Health | Isolation of glucocerebrosidase from human placental tissue |
JPS5322158B2 (de) * | 1974-05-02 | 1978-07-06 | ||
JPS53107479A (en) * | 1977-02-28 | 1978-09-19 | Green Cross Corp:The | Preparation of human lysozyme |
US4885248A (en) | 1984-02-15 | 1989-12-05 | Lubrizol Genetics, Inc. | Transfer vector |
US5173410A (en) | 1984-02-15 | 1992-12-22 | Lubrizol Genetics Inc. | Transfer vector |
US5466788A (en) | 1985-03-07 | 1995-11-14 | Mycogen Plant Science, Inc. | Subgenomic promoter |
CA1288073C (en) | 1985-03-07 | 1991-08-27 | Paul G. Ahlquist | Rna transformation vector |
DE3850683T2 (de) | 1987-02-09 | 1994-10-27 | Lubrizol Genetics Inc | Hybrides RNS-Virus. |
US5316931A (en) | 1988-02-26 | 1994-05-31 | Biosource Genetics Corp. | Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes |
DE4016562A1 (de) * | 1990-05-23 | 1991-11-28 | Degussa | Rohrverbindungsstuecke zur installation von rohrleitungen und verfahren zu ihrer herstellung |
US5852172A (en) * | 1991-06-13 | 1998-12-22 | University Of Waterloo | Cold tolerances in plants |
US5597569A (en) * | 1993-10-25 | 1997-01-28 | Bristol-Myers Squibb Company | Bryodin 2 a ribosome-inactivating protein isolated from the plant Bryonia dioica |
CH688988A5 (de) * | 1994-03-14 | 1998-07-15 | Ferrum Ag | Filterzentrifuge. |
US5837826A (en) * | 1995-02-27 | 1998-11-17 | Regents Of The University Of Minnesota | Protein adsorption by very dense porous zirconium oxide particles in expanded beds |
ES2124176B1 (es) * | 1996-10-07 | 1999-10-01 | Univ Valladolid | Procedimiento para la obtencion de proteinas inactivadoras de ribosomas, denominadas beetin 27 y beetin 29, de las hojas de beta vulgaris (remolacha azucarera) infectadas con el virus de la amarillez. |
BR9912875A (pt) * | 1998-08-11 | 2002-02-13 | Large Scale Biology Corp | Método para a recuperação de proteìnas a partir do fluido intersticial de tecidos vegetais |
-
1999
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See references of WO0009725A2 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9056894B2 (en) | 2010-10-27 | 2015-06-16 | Philip Morris Products S.A. | Methods for capturing virus like particles from plants using expanded bed chromatogrpahy |
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MXPA01001445A (es) | 2002-11-29 |
US20030073209A1 (en) | 2003-04-17 |
CA2349852A1 (en) | 2000-02-24 |
KR20010072372A (ko) | 2001-07-31 |
BR9912875A (pt) | 2002-02-13 |
ZA200101100B (en) | 2001-11-26 |
NZ510358A (en) | 2002-09-27 |
AU5396799A (en) | 2000-03-06 |
DE69933677T2 (de) | 2007-08-23 |
US6284875B1 (en) | 2001-09-04 |
WO2000009725A3 (en) | 2000-05-18 |
AU759813B2 (en) | 2003-05-01 |
US6617435B2 (en) | 2003-09-09 |
US20050059127A1 (en) | 2005-03-17 |
EP1104479B1 (de) | 2006-10-18 |
ATE342994T1 (de) | 2006-11-15 |
US6841659B2 (en) | 2005-01-11 |
US6441147B1 (en) | 2002-08-27 |
DE69933677D1 (de) | 2006-11-30 |
US7034128B2 (en) | 2006-04-25 |
JP2002522559A (ja) | 2002-07-23 |
WO2000009725A2 (en) | 2000-02-24 |
IL141312A0 (en) | 2002-03-10 |
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