EP0824592A1 - Protease alcaline et son procede de production - Google Patents

Protease alcaline et son procede de production

Info

Publication number
EP0824592A1
EP0824592A1 EP96915439A EP96915439A EP0824592A1 EP 0824592 A1 EP0824592 A1 EP 0824592A1 EP 96915439 A EP96915439 A EP 96915439A EP 96915439 A EP96915439 A EP 96915439A EP 0824592 A1 EP0824592 A1 EP 0824592A1
Authority
EP
European Patent Office
Prior art keywords
ala
nucleic acid
gly
ser
protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP96915439A
Other languages
German (de)
English (en)
Inventor
Alan P. Sloma
Helle Outtrup
Claus Dambmann
Dorrit Anita Aaslyng
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Novozymes Inc
Original Assignee
Novo Nordisk AS
Novo Nordisk Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS, Novo Nordisk Biotech Inc filed Critical Novo Nordisk AS
Publication of EP0824592A1 publication Critical patent/EP0824592A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Cette invention, qui concerne un ou plusieurs d'acides nucléiques isolés obtenus par recombinaison, comprenant une séquence nucléotidique codant une protéase alcaline d'un poids moléculaire d'environ 34kD, que l'on peut obtenir à partir d'une souche de Bacillus sp., Groupe I, traite également de vecteurs de recombinaison et de cellules hôtes comportant ces produits de recombinaison. L'invention concerne, en outre, une technique de production de cette protéase. Elle concerne également des séquences promoteur et signal dérivées de ladite protéase alcaline ainsi que des méthodes d'utilisation de ces séquences pour l'expression de séquences nucléotidiques hétérologues codant des protéines hétérologues dans des bactéries.
EP96915439A 1995-05-03 1996-05-01 Protease alcaline et son procede de production Withdrawn EP0824592A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/434,255 US5621089A (en) 1992-05-27 1995-05-03 Nucleic acid constructs for the production of a Bacillus alkaline protease
US434255 1995-05-03
PCT/US1996/006097 WO1996034963A1 (fr) 1995-05-03 1996-05-01 Protease alcaline et son procede de production

Publications (1)

Publication Number Publication Date
EP0824592A1 true EP0824592A1 (fr) 1998-02-25

Family

ID=23723485

Family Applications (1)

Application Number Title Priority Date Filing Date
EP96915439A Withdrawn EP0824592A1 (fr) 1995-05-03 1996-05-01 Protease alcaline et son procede de production

Country Status (5)

Country Link
US (4) US5621089A (fr)
EP (1) EP0824592A1 (fr)
JP (1) JPH11504813A (fr)
AU (1) AU5721296A (fr)
WO (1) WO1996034963A1 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6455304B1 (en) * 1994-07-01 2002-09-24 The Board Of Regents Of The University Of Oklahoma Hyaluronate synthase gene and uses thereof
US7091008B1 (en) * 1994-07-01 2006-08-15 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthase genes and expression thereof in Bacillus hosts
US6416756B1 (en) * 1997-01-10 2002-07-09 Novozymes A/S Modified protease having 5 to 13 covalently coupled polymeric molecules for skin care
WO1998035026A1 (fr) * 1997-02-06 1998-08-13 Novo Nordisk A/S Conjugats polypeptide-polymere a groupes de fixation ajoutes et/ou retires
CN101113436B (zh) * 1997-10-31 2013-02-06 俄克拉何马大学董事会 透明质酸合酶基因及其应用
EP1025211B1 (fr) * 1997-10-31 2004-12-15 The Board of Regents of The University of Oklahoma Gene de la synthase de l'hyaluronan et utilisation de ce gene
US6987023B2 (en) * 1998-04-02 2006-01-17 The Board Of Regents Of The University Of Oklahoma DNA encoding hyaluronan synthase from Pasteurella multocida and methods of use
US7223571B2 (en) * 1998-04-02 2007-05-29 The Board Of Regents Of The Universtiy Of Oklahoma Targeted glycosaminoglycan polymers by polymer grafting and methods of making and using same
US20080108110A1 (en) * 1998-04-02 2008-05-08 Deangelis Paul L Targeted glycosaminoglycan polymers by polymer grafting and methods of making and using same
US20060188966A1 (en) * 1998-04-02 2006-08-24 Deangelis Paul L Natural, chimeric and hybrid glycosaminoglycan polymers and methods of making and using same
US20050191741A1 (en) * 1998-09-25 2005-09-01 Council Of Scientific And Industrial Research Microbial composition and a process useful for the neutralization of alkaline waste- waters
US7094581B2 (en) * 1998-10-26 2006-08-22 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthases and methods of making and using same
US20050287638A1 (en) * 2000-04-25 2005-12-29 Weigel Paul H Hyaluronan receptor for endocytosis, variants thereof, and methods of making and using same
AU2001291649A1 (en) * 2000-10-02 2002-04-15 Novozymes A/S Nucleic acids encoding polypeptides having proteolytic activity
US20070020737A1 (en) * 2001-12-03 2007-01-25 Pummill Philip E Hyaluronan synthases and methods of making and using same
US20060236414A1 (en) 2003-06-19 2006-10-19 Novozymes A/S Proteases and methods for producing them
KR102533305B1 (ko) * 2016-10-07 2023-05-16 가부시키가이샤 에스디에스 바이오텍크 바실루스속 세균의 배양 방법 및 유용 물질의 제조 방법
CN115260314B (zh) * 2022-05-10 2023-10-24 吉林大学 一种重组嗜热中性蛋白酶及其在降解蛋白质中的应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6055118B2 (ja) * 1982-02-08 1985-12-03 昭和電工株式会社 新規な細菌アルカリプロテア−ゼ及びその製造方法
US4652639A (en) * 1982-05-06 1987-03-24 Amgen Manufacture and expression of structural genes
US4559300A (en) * 1983-01-18 1985-12-17 Eli Lilly And Company Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
BE897479A (fr) * 1983-08-08 1983-12-01 Showa Denko Kk Protease alcaline sa preparation et son utilisation
US4801537A (en) * 1984-06-08 1989-01-31 Genex Corporation Vector for expression of polypeptides in bacilli
US4771003A (en) * 1985-10-22 1988-09-13 Genex Corporation Heat stable alkaline proteases produced by a bacillus
US4764470A (en) * 1986-02-05 1988-08-16 Genex Corporation Alkaline protease produced by a bacillus
DE3642005C1 (de) * 1986-12-09 1988-03-03 Ibsm Mehltretter Gmbh Vorrichtung zum Schneiden von folienartigen Werkstoffen
EP0493398B1 (fr) * 1989-08-25 1999-12-08 Henkel Research Corporation Enzyme proteolytique alcaline et procede de production
DK58491D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
DK58391D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
DK58591D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
DK70292D0 (da) * 1992-05-27 1992-05-27 Novo Nordisk As Nyt enzym

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9634963A1 *

Also Published As

Publication number Publication date
WO1996034963A1 (fr) 1996-11-07
JPH11504813A (ja) 1999-05-11
US5621089A (en) 1997-04-15
AU5721296A (en) 1996-11-21
US5622841A (en) 1997-04-22
US5650326A (en) 1997-07-22
US5622850A (en) 1997-04-22

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19971203

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: NOVOZYMES A/S

Owner name: NOVO NORDISK BIOTECH, INC.

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: NOVOZYMES A/S

Owner name: NOVOZYMES BIOTECH, INC.

17Q First examination report despatched

Effective date: 20011115

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20020526