EP0820348B1 - Testkarte für analysen - Google Patents

Testkarte für analysen Download PDF

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Publication number
EP0820348B1
EP0820348B1 EP97901736A EP97901736A EP0820348B1 EP 0820348 B1 EP0820348 B1 EP 0820348B1 EP 97901736 A EP97901736 A EP 97901736A EP 97901736 A EP97901736 A EP 97901736A EP 0820348 B1 EP0820348 B1 EP 0820348B1
Authority
EP
European Patent Office
Prior art keywords
chambers
partition
card
card according
cavities
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP97901736A
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English (en)
French (fr)
Other versions
EP0820348A1 (de
Inventor
Bernard Jean-Marie Limon
Fabienne Marquis-Weible
Philippe Renaud
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Publication of EP0820348A1 publication Critical patent/EP0820348A1/de
Application granted granted Critical
Publication of EP0820348B1 publication Critical patent/EP0820348B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Definitions

  • the present invention relates to the treatment of a analysis card.
  • analysis is meant any process or process allowing to identify, separate, isolate, determine, detect, or quantify, a material, a product, a substance, or compound, referred to as generic "analyte", from a sample or sample to be analyzed, previously diluted or not with any suitable medium, for example a solvent.
  • the analyte sought may be chemical, biochemical, or still biological, for example in the latter case a antigen or antibody.
  • analysis card any device, module, or system, internally arranged for carry out the different processes or reactions necessary for the identification, separation, detection or quantification of the analyte, different treatments, in particular within said card, or manipulation of the analysis card, for example automatically.
  • Such an analysis card well known to those skilled in the art, constitutes a closed assembly vis-à-vis the outside or its immediate environment, with the exception of course of all passages or means equivalent, allowing in particular and initially introduce the sample or sample to be analyzed.
  • a such analysis card contains the reagent (s), physico-chemical, chemical, biochemical, or organic, distributed and maintained in the card, according to the flow of the sample to be analyzed, and the reaction processes or reactions to perform for complete the analysis.
  • An analysis card such that envisaged by the present invention, constituting in general a disposable device or assembly, i.e. thrown away or destroyed after use, understands or incorporates within it a plurality of chambers, arranged in series and / or in parallel, one of which, for example the last, is notably an optical measurement chamber.
  • These analysis cards can be produced or produced according to all appropriate techniques, for example in one or several parts assembled (for example welding) together to others, made for example by molding one or several identical or different plastics.
  • room is meant in the claims and the description of any enclosure, or passage, ensuring reception and / or circulation of any liquid, fluid, or gas present in the analysis card.
  • the present invention relates to a card particular analysis, allowing a setting operation in communication of at least two rooms, arranged in an analysis card, and initially isolated one by compared to the other, this operation being carried out at distance and no action or mechanical contact with the card analysis.
  • the present invention lies in cooperation of two essential means, namely, on the one hand a light beam, in particular coherent, having at least one predetermined wavelength ⁇ , and a power predetermined P, obtained from a light source, in particular laser, and on the other hand of a structure or arrangement of an analysis card, in which are provided at least two isolated rooms, one with respect to to the other, and to the outside; but this structure is specifically adapted to the implementation of this light beam, to establish at least one passage between at least two chambers of said card.
  • the two rooms are separated from each other by a partition, perforable, arranged within the card, in absorbent material, in particular plastic, absorbing light energy from the light beam above, to transform it into thermal energy likely to eliminate at least locally said material ; and two cavities are formed on the side and on the other side of the bulkhead, and are in communication with or included in the two rooms respectively; and an window in transparent material at least for the wavelength ⁇ is arranged opposite said partition, and delimits with the latter one of said cavities, called the incidence of a light ray.
  • the light beam used according to the invention can be convergent, parallel, or divergent, the essential condition being the density of power of the light beam striking the partition penetrable.
  • suitable guiding means can be provided before and / or after the beam, to satisfy the essential condition mentioned above.
  • the method of using the card according to the present invention allows therefore its manipulation, in particular by automatic track, driving, in relation to the beam luminous, in particular laser, at the perforation of any partition, arranged within said card, and this in preserving the rest or the integrity of the latter.
  • a such a process therefore appears particularly suitable for analysis devices today, working for a large part, if not all, automatically.
  • the analysis card 1 shown is intended for cooperate with or adapted to a laser light source 4, emitting a coherent light or light beam 5, having a predetermined wavelength ⁇ , and a power predetermined light P.
  • the analysis card 1 has a general shape substantially flattened, and includes, so assembled, for example by gluing, a flat body 14, by example polystyrene or polycarbonate wafer, previously engraved or imprinted, as described below, between two external walls, or films 15 and 16, for example in transparent plastic vis-à-vis the beam luminous.
  • films 15 and 16 can be in polypropylene, silicon or germanium.
  • two chambers 2 and 3 are obtained and included in the analysis card 1, in being, on the one hand isolated each with respect to outside the map, and on the other hand isolated at the start one over the other.
  • the two rooms 2 and 3 are separated from each other by a partition 7 that can be pierced by laser light from source 4, absorbent material, in particular plastic material, absorbing light energy of wavelength ⁇ from the laser source, to transform it into thermal energy or calorific likely to eliminate or make disappear at least locally or occasionally the material of the partition 7.
  • Two cavities 8 and 9 are provided on either side and on the other side of the partition 7, and are respectively in communication with the two rooms 2 and 3.
  • the incidence of the radius bright 12 illuminating the partition 7 is at the same time substantially perpendicular to the plane of the map 1 analysis, and corresponds to the reference direction 6 of the light beam 5 emitted by the source 4.
  • the axis of the light ray 12 illuminating the partition 7 crosses the latter substantially at its center, in providing a border on either side of the hole perforating 7a, unaffected by the light energy of the laser ray.
  • the light ray 12 illuminating the partition 7 is converging at a point focal 13 arranged in the center or within the partition 7.
  • the light ray 12 can also be, or parallel, or divergent, for the purposes of the illumination of partition 7, according to the distribution of light energy, desired in the impact zone of the light ray 12.
  • the embodiment shown in Figures 3 to 5 differs from that shown in Figures 1 to 2, by the fact that the analysis card 1 includes "n", in the occurrence 3 chambers 2, 3, 17, arranged in series, but can also be arranged in parallel, separated two to two by "n-1", in this case two partitions perforables 7 and 18, "n” being an integer, and one bedrooms 2, 3 and 17, which can in particular be a bedroom optical measurement. So as to allow a process automatic with a single laser light beam, the perforable partitions 7 and 18 are distributed in the card 1, so that two partitions cannot be aligned with a single light beam incident 12. As previously indicated, the card of analysis of Figures 3 to 5 contains a liquid filling chamber 3, which will travel towards the chamber 2, after perforation of the partition 7, as shown in Figure 4, then to room 17, after perforation of the partition 18, as partially shown in Figure 5.
  • the generator 20 emits a light at 800 nm, with guide light at 670 nm.
  • the power of the light source thus constituted is 700 mW, for a power actually used of the order 300 mW.
  • the optical fiber 21 used is a fiber multimode with a core diameter of 200 ⁇ m.
  • a lens 30 makes it possible to focus the light energy on the center 13, located on or in each partition 7 or 18.
  • the present invention can be implemented according to different modes of execution; especially one rooms, connected according to this invention, can itself communicate with the atmosphere or the environment in which the analysis card is located.

Claims (9)

  1. Testkarte für Analysen (1), in welcher zwei Kammern (2, 3) ausgespart sind, die voneinander getrennt sind, dadurch gekennzeichnet, daß die zwei Kammern durch eine perforierbare Trennwand (7) voneinander getrennt sind, wobei die Trennwand im Inneren der Testkarte angeordnet ist und aus insbesondere einem Kunststoffmaterial besteht und Lichtenergie von zumindest einer bestimmten Wellenlänge λ absorbiert und diese in eine thermische Energie umwandelt, welche ausreicht, zumindest stellenweise das Material zu entfernen, daß ferner zwei Aushöhlungen (8, 9) auf beiden Seiten der Trennwand (7) ausgespart sind, die mit den zwei Kammern (2, 3) in Verbindung stehen oder in diesen enthalten sind, daß außerdem gegenüberliegend zur Trennwand (7) ein Fenster (10, 11) aus einem zumindest für die Wellenlänge λ durchlässigem Material angeordnet ist, und daß mit. der Trennwand einer der Aushöhlungen (8) abgegrenzt ist, in welche ein Lichtstrahl (12) einfallen kann.
  2. Testkarte nach Anspruch 1, dadurch gekennzeichnet, daß ein weiteres Fenster (11) aus einem zumindest für die Wellenlänge λ durchlässigem Material gegenüberliegend zur Trennwand (7) zusammen mit der letzteren die andere Aushöhlung (9) abgrenzt, aus welcher ein Lichtstrahl austreten kann.
  3. Testkarte nach Anspruch 1, dadurch gekennzeichnet, daß sie einen Körper (14) aufweist, in welchen die zwei Kammern (2, 3) und die zwei Aushöhlungen (8, 9) eingeprägt oder eingraviert sind, und daß zumindest eine äußere Wand (15, 16) die Kammern und die Aushöhlungen nach außen abschließt.
  4. Testkarte nach Anspruch 3, mit einer im wesentlichen abgeflachten Form, dadurch gekennzeichnet, daß sie zwischen zwei äußeren Wänden (15, 16) einen flachen Körper (14) aufweist.
  5. Testkarte nach Anspruch 3, dadurch gekennzeichnet, daß eine der Kammern (2) und die ihr entsprechende Aushöhlung (8) durch eine der äußeren Wände (15) geschlossen sind, und daß die andere Kammer (3) und die ihr entsprechende andere Aushöhlung (9) durch die andere äußere Wand (16) geschlossen sind.
  6. Testkarte nach Anspruch 3, dadurch gekennzeichnet, daß die perforierbare Trennwand (7) in dem flachen Körper (14) ausgebildet ist, dessen diese aufbauendes Material Lichtenergie der Wellenlänge λ absorbiert.
  7. Testkarte nach Anspruch 3, dadurch gekennzeichnet, daß das Fenster (10) in der äußeren Wand (15) ausgebildet ist, dessen diese aufbauendes Material für die Wellenlänge λ durchlässig ist.
  8. Testkarte nach Anspruch 1, dadurch gekennzeichnet, daß sie n Kammern (2, 3, 17) aufweist, die in Serie und/oder parallel angeordnet sind, und von denen jeweils zwei durch n-1 perforierbare Trennwände (7, 18) getrennt sind, wobei "n" eine ganze Zahl bedeutet, und daß eine der Kammern insbesondere eine optische Meßkammer ist, wobei die perforierbaren Trennwände auf der Meßkarte derart verteilt sind, daß nicht zwei Trennwände mit ein und demselben einfallenden Lichtstrahl (12) in Ausrichtung stehen.
  9. Einsatzbereite Testkarte für Analysen nach Anspruch 1, wobei mindestens eine Kammer (3) mit einer Flüssigkeit gefüllt ist.
EP97901736A 1996-02-12 1997-02-12 Testkarte für analysen Expired - Lifetime EP0820348B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9601984 1996-02-12
FR9601984A FR2744803B1 (fr) 1996-02-12 1996-02-12 Procede et dispositif de traitement d'une carte d'analyse
PCT/IB1997/000112 WO1997028899A1 (fr) 1996-02-12 1997-02-12 Carte d'analyse

Publications (2)

Publication Number Publication Date
EP0820348A1 EP0820348A1 (de) 1998-01-28
EP0820348B1 true EP0820348B1 (de) 2002-01-09

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EP97901736A Expired - Lifetime EP0820348B1 (de) 1996-02-12 1997-02-12 Testkarte für analysen

Country Status (7)

Country Link
US (1) US5869002A (de)
EP (1) EP0820348B1 (de)
AT (1) ATE211657T1 (de)
CA (1) CA2218415C (de)
DE (1) DE69709499T2 (de)
FR (1) FR2744803B1 (de)
WO (1) WO1997028899A1 (de)

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US7981600B2 (en) * 2003-04-17 2011-07-19 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene
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US8834792B2 (en) 2009-11-13 2014-09-16 3M Innovative Properties Company Systems for processing sample processing devices
USD638951S1 (en) 2009-11-13 2011-05-31 3M Innovative Properties Company Sample processing disk cover
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KR101963721B1 (ko) 2011-05-18 2019-03-29 디아소린 에스.피.에이. 샘플 처리 장치 상의 밸빙을 위한 시스템 및 방법
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Also Published As

Publication number Publication date
DE69709499T2 (de) 2002-09-26
CA2218415A1 (fr) 1997-08-14
CA2218415C (fr) 2006-05-30
WO1997028899A1 (fr) 1997-08-14
DE69709499D1 (de) 2002-02-14
FR2744803A1 (fr) 1997-08-14
ATE211657T1 (de) 2002-01-15
EP0820348A1 (de) 1998-01-28
US5869002A (en) 1999-02-09
FR2744803B1 (fr) 1998-03-13

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