EP0807247A1 - Method and apparatus for detecting fluorophore labelled substances - Google Patents

Method and apparatus for detecting fluorophore labelled substances

Info

Publication number
EP0807247A1
EP0807247A1 EP96902044A EP96902044A EP0807247A1 EP 0807247 A1 EP0807247 A1 EP 0807247A1 EP 96902044 A EP96902044 A EP 96902044A EP 96902044 A EP96902044 A EP 96902044A EP 0807247 A1 EP0807247 A1 EP 0807247A1
Authority
EP
European Patent Office
Prior art keywords
fluorophore
wavelength
laser
excitation
emitting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP96902044A
Other languages
German (de)
English (en)
French (fr)
Inventor
Bengt-Göran Andersson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Pharmacia Biotech AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Biotech AB filed Critical Pharmacia Biotech AB
Publication of EP0807247A1 publication Critical patent/EP0807247A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
EP96902044A 1995-02-01 1996-01-31 Method and apparatus for detecting fluorophore labelled substances Withdrawn EP0807247A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9500361A SE9500361D0 (sv) 1995-02-01 1995-02-01 Förfarande och anordning för detektering av fluoroformärkta substanser
SE9500361 1995-02-01
PCT/SE1996/000109 WO1996024042A1 (en) 1995-02-01 1996-01-31 Method and apparatus for detecting fluorophore labelled substances

Publications (1)

Publication Number Publication Date
EP0807247A1 true EP0807247A1 (en) 1997-11-19

Family

ID=20397051

Family Applications (1)

Application Number Title Priority Date Filing Date
EP96902044A Withdrawn EP0807247A1 (en) 1995-02-01 1996-01-31 Method and apparatus for detecting fluorophore labelled substances

Country Status (4)

Country Link
EP (1) EP0807247A1 (sv)
JP (1) JPH10513555A (sv)
SE (1) SE9500361D0 (sv)
WO (1) WO1996024042A1 (sv)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0814594B1 (en) * 1996-06-18 2004-09-08 Fuji Photo Film Co., Ltd. Image reading apparatus
JP4812393B2 (ja) 2005-03-04 2011-11-09 株式会社日立ハイテクノロジーズ 蛍光分子計測システム

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5207880A (en) * 1984-03-29 1993-05-04 The Board Of Regents Of The University Of Nebraska DNA sequencing
US4675300A (en) * 1985-09-18 1987-06-23 The Board Of Trustees Of The Leland Stanford Junior University Laser-excitation fluorescence detection electrokinetic separation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9624042A1 *

Also Published As

Publication number Publication date
JPH10513555A (ja) 1998-12-22
SE9500361D0 (sv) 1995-02-01
WO1996024042A1 (en) 1996-08-08

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19970718

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): DE FR GB

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: APBIOTECH AKTIEBOLAG

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: AMERSHAM BIOSCIENCES AB

17Q First examination report despatched

Effective date: 20030603

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20031014