EP0787292A1 - Method and arrangement in a gel electrophoresis apparatus - Google Patents
Method and arrangement in a gel electrophoresis apparatusInfo
- Publication number
- EP0787292A1 EP0787292A1 EP95936156A EP95936156A EP0787292A1 EP 0787292 A1 EP0787292 A1 EP 0787292A1 EP 95936156 A EP95936156 A EP 95936156A EP 95936156 A EP95936156 A EP 95936156A EP 0787292 A1 EP0787292 A1 EP 0787292A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- light beam
- electrophoresis gel
- gel
- electrophoresis
- incidence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
Definitions
- the invention relates to a method and an arrangement in a gel electrophoresis apparatus having a light source whose light beam is to be introduced into an electrophoresis gel through one of its lateral edges to enable detection of at least one component which is migrating in the electrophoresis gel and which is labelled with a fluorescent substance.
- the light source e.g. the laser
- the object of the invention is to eliminate the manual fine adjustment mentioned above and instead bring about a gel electrophoresis apparatus in which the fine adjustment is done automatically. forth across said one lateral edge of the electrophoresis gel to introduce the light beam into the electrophoresis gel at different points of incidence, that the intensity of the light beam introduced at said different points of incidence is measured after that it, at least partially, has passed through the electrophoresis gel, and that the light beam is locked at the point of incidence at which the highest intensity is measured.
- the object is attained by means of the arrangement according to the invention, mainly, in that a means is adapted to sweep the light beam back and forth across said one lateral edge of the electrophoresis gel to introduce the light beam into the electrophoresis gel at different points of incidence, and that a sensor is adapted to measure the intensity of the light beam introduced at said different points of incidence after that it, at least partially, has passed through the electrophoresis gel, and to lock the light beam at the point of incidence at which the highest intensity is measured.
- FIG. 1 schematically shows a block diagram of an embodiment of an arrangement according to the invention at an electrophoresis gel, a top end view thereof being shown.
- Fig. 1 shows a top end view of a vertical electrophoresis gel 1 between glass plates 2 and 3 which are separated by means of spacers 4 and 5 which both are translucent in the embodiment shown.
- the shown gel 1 is intended for separation of a mixture of components, e.g. nucleic acids, and is, in a manner known per se, connected to an upper buffer liquid container (not shown) and a lower buffer liquid container (not shown) of an electrophoresis apparatus.
- a mixture of components e.g. nucleic acids
- the samples thereof labelled with fluorescent substances
- the sample components migrate downwards in the gel 1 under influence of an electric field.
- a light beam which is a laser beam 6 in the embodiment shown, is introduced through the translucent spacer 4, and into the gel 1 through one of its lateral edges in order to enable detection of the above-mentioned samples migrating in the gel 1 by means of detectors (not shown) .
- the laser beam 6 is generated by a laser 7 and introduced into the gel 1 by means of a mirror 8.
- a motor 9 is provided to move the mirror 8 back and forth across the lateral edge of the gel 1 as indicated by arrow 10 in order to sweep the laser beam 6 back and forth across the lateral edge of the spacer 4 and, thereby, the gel 1 as indicated by means of arrow 11 in order to introduce the laser beam 6 into the gel 1 at different points of incidence.
- the motor 9 for moving the mirror 8 is actuated by means of a control unit 12 which is provided with a starting button (not shown) .
- the laser beam 6 coming from the mirror 8 passes through the gel 1 and exits, in the embodiment shown, from the gel 1 through its other lateral edge via the translucent spacer 5.
- a sensor 13 is provided at the other lateral edge of the gel 1, which sensor 13 is adapted to measure the light-intensity of the laser beam 6 during the sweeping motion of the laser beam 6, and produce a signal which is proportional to the light-intensity, and which is supplied to the control unit 12 which in its turn is adapted to stop the motor 9 and, thereby, the mirror 8 in the position for which the sensor 13 has measured the highest light-intensity of the laser beam 6.
- the laser beam 6 is automatically set to the point of incidence for which the laser light, exiting from the gel 1, exhibits a light-intensity maximum.
- Gel electrophoresis apparatuses are also known, in which the laser beam is introduced into the gel from the laser via a spacer without any mirror.
- the motor corresponding to the motor 9 shown in the drawing has to be adapted to move the laser instead of the mirror in order to introduce the laser beam at different points of incidence across one of the lateral edges of the gel.
- this embodiment (not shown) corresponds to the embodiment shown on the drawing. Measuring or sensing the light-intensity of the laser beam 6 does not necessarily have to be carried out in the manner shown on the drawing, i.e. with the sensor 13 located at the other lateral edge of the gel 1.
- the senor 14 can e.g. be adapted to measure the intensity of the light that is scattered by the spacer 5 in case this spacer is opaque, while the sensor 15, in accordance with a further embodiment, can be adapted to measure the intensity of the light scattered in the gel 1 after that the laser beam, at least partially, has passed through the gel 1.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9403597A SE503503C2 (sv) | 1994-10-20 | 1994-10-20 | Förfarande och anordning för automatisk inställning av en ljusstråle vid en gelelektroforesapparat |
SE9403597 | 1994-10-20 | ||
PCT/SE1995/001229 WO1996012948A1 (en) | 1994-10-20 | 1995-10-19 | Method and arrangement in a gel electrophoresis apparatus |
Publications (1)
Publication Number | Publication Date |
---|---|
EP0787292A1 true EP0787292A1 (en) | 1997-08-06 |
Family
ID=20395689
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP95936156A Withdrawn EP0787292A1 (en) | 1994-10-20 | 1995-10-19 | Method and arrangement in a gel electrophoresis apparatus |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0787292A1 (sv) |
JP (1) | JPH10507830A (sv) |
SE (1) | SE503503C2 (sv) |
WO (1) | WO1996012948A1 (sv) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19948391A1 (de) * | 1999-10-07 | 2001-04-12 | Europ Lab Molekularbiolog | Elektrophorese-Einrichtung zum Analysieren von markierten Molekülen, insbesondere biologischen Molekülen |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3618605A1 (de) * | 1986-06-03 | 1987-12-10 | Europ Lab Molekularbiolog | Vorrichtung zur detektion von zur photonenemission anregbaren stoffen |
JPH0743353B2 (ja) * | 1990-05-31 | 1995-05-15 | 株式会社島津製作所 | 蛍光検出型ゲル電気泳動装置 |
-
1994
- 1994-10-20 SE SE9403597A patent/SE503503C2/sv not_active IP Right Cessation
-
1995
- 1995-10-19 EP EP95936156A patent/EP0787292A1/en not_active Withdrawn
- 1995-10-19 JP JP8513826A patent/JPH10507830A/ja not_active Ceased
- 1995-10-19 WO PCT/SE1995/001229 patent/WO1996012948A1/en not_active Application Discontinuation
Non-Patent Citations (1)
Title |
---|
See references of WO9612948A1 * |
Also Published As
Publication number | Publication date |
---|---|
SE503503C2 (sv) | 1996-06-24 |
SE9403597D0 (sv) | 1994-10-20 |
SE9403597L (sv) | 1996-04-21 |
WO1996012948A1 (en) | 1996-05-02 |
JPH10507830A (ja) | 1998-07-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 19970329 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): DE GB |
|
RAP1 | Party data changed (applicant data changed or rights of an application transferred) |
Owner name: APBIOTECH AKTIEBOLAG |
|
17Q | First examination report despatched |
Effective date: 20010412 |
|
RAP1 | Party data changed (applicant data changed or rights of an application transferred) |
Owner name: AMERSHAM BIOSCIENCES AB |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20040501 |