EP0708782A1 - Sonde d'adn specifique du sexe pour psittacides, procedes et equipement - Google Patents
Sonde d'adn specifique du sexe pour psittacides, procedes et equipementInfo
- Publication number
- EP0708782A1 EP0708782A1 EP94922588A EP94922588A EP0708782A1 EP 0708782 A1 EP0708782 A1 EP 0708782A1 EP 94922588 A EP94922588 A EP 94922588A EP 94922588 A EP94922588 A EP 94922588A EP 0708782 A1 EP0708782 A1 EP 0708782A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- dna
- probe
- recited
- seq
- sex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241000287530 Psittaciformes Species 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims description 65
- 239000000523 sample Substances 0.000 title claims description 34
- 239000003298 DNA probe Substances 0.000 claims abstract description 31
- 241000287531 Psittacidae Species 0.000 claims description 45
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 20
- 108020004518 RNA Probes Proteins 0.000 claims description 16
- 239000003391 RNA probe Substances 0.000 claims description 16
- 108020003215 DNA Probes Proteins 0.000 claims description 14
- 210000000349 chromosome Anatomy 0.000 claims description 13
- 239000002773 nucleotide Substances 0.000 claims description 11
- 125000003729 nucleotide group Chemical group 0.000 claims description 11
- 229960002685 biotin Drugs 0.000 claims description 10
- 235000020958 biotin Nutrition 0.000 claims description 10
- 239000011616 biotin Substances 0.000 claims description 10
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 7
- 241001465754 Metazoa Species 0.000 claims description 3
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims description 3
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 2
- 229940113082 thymine Drugs 0.000 claims description 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 16
- 239000002751 oligonucleotide probe Substances 0.000 claims 16
- 238000003556 assay Methods 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 108020004414 DNA Proteins 0.000 description 130
- 239000002585 base Substances 0.000 description 61
- 108020004707 nucleic acids Proteins 0.000 description 44
- 102000039446 nucleic acids Human genes 0.000 description 44
- 150000007523 nucleic acids Chemical class 0.000 description 44
- 239000012634 fragment Substances 0.000 description 29
- 241000578498 Psittacus erithacus Species 0.000 description 23
- 210000004369 blood Anatomy 0.000 description 23
- 239000008280 blood Substances 0.000 description 23
- 241000894007 species Species 0.000 description 23
- 241000271566 Aves Species 0.000 description 22
- 239000000243 solution Substances 0.000 description 22
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 18
- 239000004677 Nylon Substances 0.000 description 13
- 229920001778 nylon Polymers 0.000 description 13
- 108091028043 Nucleic acid sequence Proteins 0.000 description 12
- 239000011780 sodium chloride Substances 0.000 description 9
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
- 210000003746 feather Anatomy 0.000 description 8
- 238000009396 hybridization Methods 0.000 description 7
- 210000000056 organ Anatomy 0.000 description 7
- 230000001850 reproductive effect Effects 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 5
- 210000003765 sex chromosome Anatomy 0.000 description 5
- 241000272517 Anseriformes Species 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 4
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 4
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000000376 autoradiography Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 241000287524 Ara ararauna Species 0.000 description 3
- 241000726096 Aratinga Species 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241000721683 Lorius garrulus Species 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
- 239000008346 aqueous phase Substances 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 230000002285 radioactive effect Effects 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 3
- 210000003462 vein Anatomy 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000726086 Amazona aestiva Species 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241001253191 Cacatua galerita Species 0.000 description 2
- 238000007399 DNA isolation Methods 0.000 description 2
- 108010067770 Endopeptidase K Proteins 0.000 description 2
- 102100036263 Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial Human genes 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- 101001001786 Homo sapiens Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial Proteins 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 239000004141 Sodium laurylsulphate Substances 0.000 description 2
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000001949 anaesthesia Methods 0.000 description 2
- 230000037005 anaesthesia Effects 0.000 description 2
- 239000002981 blocking agent Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 229920000669 heparin Polymers 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 241000749858 Ara macao Species 0.000 description 1
- 241000749842 Aratinga solstitialis Species 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- WDPFQABQVGJEBZ-MAKOZQESSA-N Bothermon Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1.O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 WDPFQABQVGJEBZ-MAKOZQESSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241001494286 Calyptorhynchus banksii Species 0.000 description 1
- 206010053567 Coagulopathies Diseases 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 241000749936 Eolophus roseicapillus Species 0.000 description 1
- 241001504495 Erithacus Species 0.000 description 1
- 241000272496 Galliformes Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 235000000177 Indigofera tinctoria Nutrition 0.000 description 1
- 241001625930 Luria Species 0.000 description 1
- 241000721576 Melopsittacus undulatus Species 0.000 description 1
- 101100505735 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cot-2 gene Proteins 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 241000721631 Nymphicus hollandicus Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 241000722370 Pionus Species 0.000 description 1
- 241001253180 Platycercus eximius Species 0.000 description 1
- 241001250178 Probosciger aterrimus Species 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000726095 Psittacara erythrogenys Species 0.000 description 1
- 241000614968 Psittacula eupatria Species 0.000 description 1
- 241000287522 Psittacula krameri Species 0.000 description 1
- 241000579861 Psittrichas fulgidus Species 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- IYKNGWWPNRDDHK-ONEGZZNKSA-N [[5-[2,4-dioxo-5-[(e)-3-[5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoylamino]prop-1-enyl]pyrimidin-1-yl]-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O1C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)CC1N1C(=O)NC(=O)C(\C=C\CNC(=O)CCCCC2C3NC(=O)NC3CS2)=C1 IYKNGWWPNRDDHK-ONEGZZNKSA-N 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 230000002547 anomalous effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 239000003593 chromogenic compound Substances 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 210000003555 cloaca Anatomy 0.000 description 1
- 230000035602 clotting Effects 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- PGPCAHBIGRCDSV-UHFFFAOYSA-M dodecyl(trimethyl)azanium;sodium;bromide Chemical compound [Na].[Br-].CCCCCCCCCCCC[N+](C)(C)C PGPCAHBIGRCDSV-UHFFFAOYSA-M 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 229960005309 estradiol Drugs 0.000 description 1
- 229930182833 estradiol Natural products 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000003163 gonadal steroid hormone Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 229940097275 indigo Drugs 0.000 description 1
- COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- -1 phosphate ester Chemical class 0.000 description 1
- 150000003014 phosphoric acid esters Chemical class 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000020509 sex determination Effects 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229960003604 testosterone Drugs 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
- 210000004906 toe nail Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- JLEXUIVKURIPFI-UHFFFAOYSA-N tris phosphate Chemical compound OP(O)(O)=O.OCC(N)(CO)CO JLEXUIVKURIPFI-UHFFFAOYSA-N 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6879—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Nouvelles sondes d'ADN permettant de déterminer le sexe des psittacidés dans les programmes de reproduction en captivité et dans les populations à l'état sauvage. Les sondes d'ADN uniques de la présente invention sont spécifiques du sexe mais non spécifiques de l'espèce de psittacidé. Les sondes d'ADN selon l'invention sont en outre fiables, donnent des résultats en 24 heures environ et sont sans danger pour les psittacidés testés.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US93198 | 1987-09-04 | ||
| US9319893A | 1993-07-15 | 1993-07-15 | |
| PCT/US1994/008023 WO1995002605A1 (fr) | 1993-07-15 | 1994-07-15 | Sonde d'adn specifique du sexe pour psittacides, procedes et equipement |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP0708782A1 true EP0708782A1 (fr) | 1996-05-01 |
| EP0708782A4 EP0708782A4 (fr) | 1997-01-02 |
Family
ID=22237701
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP94922588A Withdrawn EP0708782A4 (fr) | 1993-07-15 | 1994-07-15 | Sonde d'adn specifique du sexe pour psittacides, procedes et equipement |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0708782A4 (fr) |
| AU (1) | AU7364594A (fr) |
| CA (1) | CA2167295A1 (fr) |
| WO (1) | WO1995002605A1 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPN283195A0 (en) * | 1995-05-05 | 1995-06-01 | Australian Water Technologies Pty Ltd | Method for the detection of viable cryptosporidium parvum cells |
-
1994
- 1994-07-15 CA CA002167295A patent/CA2167295A1/fr not_active Abandoned
- 1994-07-15 AU AU73645/94A patent/AU7364594A/en not_active Abandoned
- 1994-07-15 EP EP94922588A patent/EP0708782A4/fr not_active Withdrawn
- 1994-07-15 WO PCT/US1994/008023 patent/WO1995002605A1/fr not_active Application Discontinuation
Non-Patent Citations (2)
| Title |
|---|
| NUCLEIC ACIDS RESEARCH, vol. 20, no. 19, 11 October 1992, OXFORD GB, pages 5235-5236, XP002017078 C.Y.MIYAKI ET AL.: "Sex Typing of Aratinga Parrots Using the Human Minisatellite Probe 33.15." * |
| See also references of WO9502605A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU7364594A (en) | 1995-02-13 |
| CA2167295A1 (fr) | 1995-01-26 |
| EP0708782A4 (fr) | 1997-01-02 |
| WO1995002605A1 (fr) | 1995-01-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5480774A (en) | Determination of genomic sex in salmonids | |
| US5580971A (en) | Fungal detection system based on rRNA probes | |
| JPH03502640A (ja) | 歯周囲病原体検出用オリゴヌクレオチドプローブ | |
| JP2001507929A (ja) | 分子コーミングによる遺伝病の診断法および診断用キット | |
| JPH04502855A (ja) | 正常部位抑制ハイブリダイゼーシヨンおよびその使用 | |
| WO1984001174A1 (fr) | Identification de micro-organismes | |
| US5679514A (en) | Method for determining the sex of birds | |
| Longmire et al. | Gender identification in birds using microsatellite DNA fingerprint analysis | |
| US20050202476A1 (en) | Detection of microorganisms | |
| KR100441471B1 (ko) | 돼지의외피색유전자형결정방법 | |
| Tham et al. | Detection of chicken anaemia agent DNA sequences by the polymerase chain reaction | |
| AU753665B2 (en) | Method for detecting the presence of biological matters of bovine origin, and oligonucleotides for its implementation | |
| WO1991000926A1 (fr) | Quantification de bacteries au moyen d'un dosage d'hybridation d'acide nucleique | |
| JP2006500010A5 (fr) | ||
| EP0708782A1 (fr) | Sonde d'adn specifique du sexe pour psittacides, procedes et equipement | |
| WO1996022302A1 (fr) | Sondes d'adn specifiques au sexe pour perroquets, procedes et materiels associes | |
| JP2009005698A (ja) | イヌ科動物の遺伝子型決定のためのマイクロサテライト配列 | |
| EP0807689B1 (fr) | Procede de detection cellulaire | |
| JP2005532818A5 (fr) | ||
| US5728522A (en) | Oligonucleotides and nucleic acids for detection of ureaplasma urealyticum | |
| MacPherson et al. | Ribosomal RNA sequences for the specific detection of Toxoplasma gondii by hybridization assay | |
| Nekrutenko et al. | Representational difference analysis to distinguish cryptic species | |
| WO1996000234A1 (fr) | Sondes d'hybridation centromeres | |
| US5679513A (en) | Method for diagnosing ratites | |
| Medon et al. | Identification of enterotoxigenic Escherichia coli isolates with enzyme-labeled synthetic oligonucleotide probes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
| 17P | Request for examination filed |
Effective date: 19960203 |
|
| AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE |
|
| A4 | Supplementary search report drawn up and despatched |
Effective date: 19961114 |
|
| AK | Designated contracting states |
Kind code of ref document: A4 Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN |
|
| 18W | Application withdrawn |
Withdrawal date: 19980327 |