Classifications

• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/39—Organic or inorganic per-compounds
  • C11D3/3902—Organic or inorganic per-compounds combined with specific additives
  • C11D3/3905—Bleach activators or bleach catalysts
  • C11D3/3907—Organic compounds
  • C11D3/391—Oxygen-containing compounds
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
  • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
  • B01J13/02—Making microcapsules or microballoons
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
  • C11D17/0039—Coated compositions or coated components in the compositions, (micro)capsules
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/39—Organic or inorganic per-compounds
  • C11D3/3902—Organic or inorganic per-compounds combined with specific additives
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/39—Organic or inorganic per-compounds
  • C11D3/3902—Organic or inorganic per-compounds combined with specific additives
  • C11D3/3905—Bleach activators or bleach catalysts
  • C11D3/3907—Organic compounds
  • C11D3/3917—Nitrogen-containing compounds
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/005—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08

Definitions

• the present invention relates to a method of reducing the odour of micro-organism cells.
• the invention also relates to the use of the resultant deodorised cells in a process for encapsulating a material, in which micro-organism cells are contacted with the said material, which material is in liquid form, whereby the said material is absorbed through the micro-organism cell wall and retained within the micro ⁇ organism cells.
• the invention also relates to liquids that have been encapsulated in that manner. Background to the Invention The encapsulation of materials within micro-organism cells is well known.
• EP-B-0,085,805 (Dunlop Limited) a method of encapsulation is described in which the micro-organism is contacted with an organic lipid-extending substance that is a solvent, or a micro-dispersing medium, for the material to be encapsulated, and simultaneously and/or subsequently the micro-organism is contacted with the material to be encapsulated, said material being employed as a solution or micro-dispersion in the organic lipid-extending substance, or in a further organic lipid-extending substance or in a liquid that is miscible with the first-mentioned lipid- extending substance, whereby both the organic lipid- extending substance and the material to be encapsulated are taken into and retained passively within the micro-organism.
• an organic lipid-extending substance that is a solvent, or a micro-dispersing medium
• Suitable micro-organisms include yeasts and suitable lipid- extending substances include aliphatic alcohols, esters, aromatic hydrocarbons and hydrogenated aromatic hydrocarbons.
• An example of a material that can be encapsulated is a leuco dye suitable for use in "carbonless" copy paper.
• a stated advantage of the method described in that European patent is that, in contrast to certain earlier proposals (cf. US-A-4,001,480 and FR-A-2,179,528) , use may be made of micro-organisms having a natural lipid content of less than 40 percent by weight, without the need to employ a plasmolyser.
• EP-A-0,242,135 discloses a method of producing an encapsulated material by contacting the material in liquid form with a grown intact micro-organism having a microbial lipid content of less than 40 percent by weight.
• the encapsulatable material must be capable of diffusing into the microbial cell without causing total lysation thereof and the treatment of the micro-organism is carried out in the absence of an organic lipid-extending substance as solvent or icrodispersant for the encapsulatable material and in the absence of a plasmolyser.
• the material is absorbed by the micro-organism - typically a yeast - by diffusion across the microbial cell wall and is passively retained within the micro-organism.
• encapsulatable materials including essential oils used as flavours or fragrances, leuco dyes, vitamins, detergents such as lauryl ether sul ate, food colourants, and pesticides and the like.
• EP-A-0,414,282 discloses bleach compositions, including laundry detergents, laundry bleaches and dishwashing or scouring products, that contain a perfume
• EP-A-0,414,283 discloses fabric-softening compositions that contain a perfume.
• the perfume is encapsulated in micro-organism cells according to the conventional methods, as described in US-A-4,001,480 or EP-A-0,242,135.
• micro-organism cells for encapsulation purposes
• they may have a disagreeable odour and possibly also an unpleasing colour and may therefore diminish the acceptability of the encapsulated products, or compositions containing them, to consumers.
• micro-organism cells may be at least partially deodorised by treatment with a peroxygen bleach whilst leaving the cells at least largely intact and hence suitable for encapsulation purposes.
• the present invention in one of its aspects, accordingly provides a method of reducing the odour of micro-organism cells, characterised in that the said cells are treated with a peroxygen bleach.
• the invention also provides, in another of its aspects, a process for encapsulating a material in which micro-organism cells are contacted with the said material, which material is in liquid form, whereby the said material is absorbed through the micro-organism cell wall and retained within the micro-organism cells, characterised in that the micro ⁇ organism cells are also treated with a peroxygen bleach.
• bacteria and algae may be suitable, preferred micro-organisms are fungi, especially filamentous fungi, e.g. Aspergillus niger, and more especially the yeasts.
• yeasts which may be used in the present invention are Lipomyces species, such as L . lipofer and L. star eyi , Trichosporon species, such as T. pull lans and T. c taneum, Candida species such as C. c rvata and C. utilis ,
• Kluvveromyces fragilis and Saccharomyces cerevisiae Usually, use will be made of micro-organism cells in grown form, i.e. that have been harvested from a culture medium.
• the cells should be intact, that is to say not have undergone any significant lysation, and are preferably of large size, typically with an average diameter for the cell of from 5 to 20 ⁇ m. It is also desirable that the cells should not undergo lysation before or during the encapsulation step.
• a micro-organism will normally be chosen that under the conditions of the intended use will disintegrate or undergo sufficient disruption or permit diffusion of its contents so that the encapsulated liquid will be released at the appropriate point of application.
• the micro-organism cells are treated with a peroxygen bleach, for example peracids (including so-called 'low-activity' peracids), such as peroxymonosulfuric acid, m-chloroperbenzoic acid, diperisophthalic acid and monoperphthalic acid, and their derivatives, e.g. salts such as potassium monopersulfate (“oxone”) or magnesium monoperoxyphthalate (“H48”) .
• oxone potassium monopersulfate
• H48 magnesium monoperoxyphthalate
• Compounds that release hydrogen peroxide when dissolved in water come into consideration, especially those that are commercially available; these includes such salts as perborates, notably sodium perborate, and such adducts as percarbonates.
• the preferred peroxygen bleach is hydrogen peroxide (H 2 0 2 ) .
• the peroxygen bleach attacks the amine centres in the micro-organism.
• the treatment with the peroxygen bleach should be carried out under conditions that achieve a significant reduction in, or even complete removal of, the characteristic micro-organism odour and/or colour without being so severe as to cause any significant lysation or disruption of the cell walls (which would impair the effectiveness of the micro-organism as an encapsulation material) .
• the micro-organism is treated with an aqueous solution of the peroxygen bleach, especially one containing the peroxygen bleach at a concentration of from 0.01 to 10%, more preferably from 0.02 to 5% and most preferably from 0.1 to 2%, w/v.
• the amount of peroxygen bleach is usually 0.02 to 100%, preferably 0.04 to 50% and more preferably 0.1 to 20% by weight.
• the solution of peroxygen bleach is preferably prepared in deionised water.
• the peroxygen bleach-containing treatment solution is preferably alkaline and will typically contain an alkali - usually an alkali metal or alkaline earth metal hydroxide or carbonate, preferably sodium hydroxide - at a concentration from 0 to 2.0 M, preferably from 0.01 to 1.0 M, more preferably from 0.05 to 0.5 M. Simply buffering the solution at high pH may also be considered.
• the peroxygen bleach- containing solution contains sodium silicate, preferably in an amount from 20 to 60g/l, typically from 30 to 50g/l.
• the sodium silicate is useful as a filtering aid, is a source of alkalinity, acts as a defoaming agent and provides some control over heavy metals that might decompose the peroxygen bleach.
• other silicates e.g. other alkali metal silicates, come into consideration for use with or instead of the sodium silicate, as do filtration aids, r such as Kieselguhr or Celite, and chelating agents, such as
• EDTA ethylenediamine tetraacetic acid
• STP sodium tripolyphosphate
• the primary purpose in treating the micro-organism cells with the peroxygen bleach is to reduce or eliminate any odour of the micro-organism, the treated micro-organism
• micro-organism 25 may also be referred to hereinafter as a "bleached" micro ⁇ organism; it has been found that the reduction in odour is often accompanied by a lightening of the colour of the micro-organism cell material.
• the treated micro-organism may be employed for the
• the material to be encapsulated should be in liquid form under the conditions at which encapsulation is carried out.
• Materials that are not themselves liquid under those conditions may be used in the form of a solution or micro dispersion in a suitable solvent or dispersant, usually a solvent that is immiscible with lipid that may be present in the micro-organism.
• suitable solvents include C. - C. alcohols, e.g. methanol, ethanol or isopropanol, and the solvent may be removed by evaporation after the encapsulation treatment. It is also possible, and sometimes preferred, to carry out the encapsulation process in the presence of water.
• encapsulation could be effected from a system containing both the peroxygen bleach in aqueous solution and the material to be encapsulated, provided that the material to be encapsulated were compatible with that bleach.
• the present invention is particularly advantageous in the encapsulation of bleach activators used in cleaning compositions, for example heavy duty or general purpose laundry detergent compositions, bleaching compositions, dishwashing compositions and hard-surface or other cleaning products.
• Such bleach activators are commonly susceptible to attack by moisture, leading to hydrolysis or premature perhydrol sis, the products of which are liable to damage other ingredients in the cleaning composition.
• Suitable bleach activators are disclosed in U.S. Patents No. 4,179,390 (Spadini et al.) , No. 4,412,934 (Chung et al.) and No. 4,915,854 (Mao et al.)
• the present invention is illustrated in and by the following examples.
• Example 1 fa) Yeast Treatment lOOg of baker's yeast (Saccharomyces cerevisiae) were suspended in one litre of a 0.2 molar solution of sodium hydroxide in water containing 40g of sodium silicate.
• Example 1 A number of samples of bleached yeast micro-capsules were prepared using the process described above in Example 1 (a) but with certain variations in the yeast concentration, alkalinity, the presence or absence of sodium silicate, and the concentration of hydrogen peroxide. The samples were assessed for yeast odour and for the amount of foaming produced during the hydrogen peroxide treatment. The results are summarised in the following table (in which sample 5 indicates the sample obtained by the process according to Example 1(a)).
• the cell membrane For the yeast cells to be useful for encapsulation purposes, the cell membrane must be intact. Microscopy showed that this was only the case for Samples 5 and 6.
• Sample 5 was preferred to Sample £, in that it exhibited a lower odour and lower foaming.
• compositions were sampled after certain periods of time, the samples being analyzed in order to determine how much of the bleach activator (acetyl triethyl citrate or TAED, as the case may be) remained (expressed as a percentage of the original content) .
• the analysis was effected by dissolving the sample, analyzing for peracid and comparing that result with the expected result for 100% active. The results are shown in the following table. Table 2
• the 5-week result for the bleached capsules is anomalous and is thought to be due to poor dispersion of the product; in particular, caking, which is a problem commonly experienced

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• Chemical & Material Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Organic Chemistry (AREA)
• Engineering & Computer Science (AREA)
• Wood Science & Technology (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Oil, Petroleum & Natural Gas (AREA)
• Inorganic Chemistry (AREA)
• Health & Medical Sciences (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Genetics & Genomics (AREA)
• Biotechnology (AREA)
• Zoology (AREA)
• Medicinal Chemistry (AREA)
• Biochemistry (AREA)
• Tropical Medicine & Parasitology (AREA)
• Biomedical Technology (AREA)
• Sustainable Development (AREA)
• Microbiology (AREA)
• Dispersion Chemistry (AREA)
• Virology (AREA)
• General Engineering & Computer Science (AREA)
• General Health & Medical Sciences (AREA)
• Detergent Compositions (AREA)
• Manufacturing Of Micro-Capsules (AREA)
• Micro-Organisms Or Cultivation Processes Thereof (AREA)
• Food Preservation Except Freezing, Refrigeration, And Drying (AREA)

Priority Applications (1)

Applications Claiming Priority (4)

Publications (2)

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Family Applications (1)

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• 1992
  • 1992-11-19 TR TR01115/92A patent/TR27620A/tr unknown
  • 1992-12-01 CA CA 2124791 patent/CA2124791C/en not_active Expired - Fee Related
  • 1992-12-01 EP EP93900776A patent/EP0672113A1/en not_active Withdrawn
  • 1992-12-01 JP JP51095993A patent/JP3222136B2/ja not_active Expired - Fee Related
  • 1992-12-01 WO PCT/US1992/010391 patent/WO1993011869A1/en not_active Application Discontinuation
  • 1992-12-09 MA MA23022A patent/MA22732A1/fr unknown
  • 1992-12-11 MX MX9207184A patent/MX9207184A/es unknown
  • 1992-12-12 CN CN 92114897 patent/CN1074481A/zh active Pending

Patent Citations (4)

Non-Patent Citations (2)

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