EP0549692A1 - Sequences d'oligonucleotides specifiques de sequences de proteines - Google Patents

Sequences d'oligonucleotides specifiques de sequences de proteines

Info

Publication number
EP0549692A1
EP0549692A1 EP91917153A EP91917153A EP0549692A1 EP 0549692 A1 EP0549692 A1 EP 0549692A1 EP 91917153 A EP91917153 A EP 91917153A EP 91917153 A EP91917153 A EP 91917153A EP 0549692 A1 EP0549692 A1 EP 0549692A1
Authority
EP
European Patent Office
Prior art keywords
oligonucleotide
mixture
sequence
sequences
oligonucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP91917153A
Other languages
German (de)
English (en)
Other versions
EP0549692A4 (en
Inventor
Harold Martin Weintraub
Thomas Keith Blackwell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fred Hutchinson Cancer Center
Original Assignee
Fred Hutchinson Cancer Research Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fred Hutchinson Cancer Research Center filed Critical Fred Hutchinson Cancer Research Center
Publication of EP0549692A1 publication Critical patent/EP0549692A1/fr
Publication of EP0549692A4 publication Critical patent/EP0549692A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Abstract

Méthode servant à identifier des séquences d'oligomères qui se lient spécifiquement à des protéines cibles ou à d'autres fractions cibles. La technique comprend la complexation de la substance cible avec un mélange d'oligonucléotides contenant des séquences aléatoires et des séquences qui servent d'amorces pour des réactions en chaîne de polymérases dans des conditions dans lesquelles un complexe se forme avec certains éléments, mais pas tous, du mélange d'oligonucléotides. Le mélange est ensuite séparé des oligonucléotides non complexés et les éléments complexés du mélange d'oligonucléotides sont récupérés du complexe séparé et amplifiés en utilisant la réaction en chaîne de polymérase. On peut procéder à plusieurs tours de sélection en utilisant la complexation, la séparation, l'amplification et la récupération. Le mélange ou les oligonucléotides récupérés peuvent être mis en séquences et cette information peut être utilisée pour la synthèse de novo du(des) oligonucléotide(s) identifié(s). Ces oligonucléotides synthétisés peuvent contenir une ou plusieurs bases ou liaisons de squelette modifiées.
EP19910917153 1990-09-21 1991-09-18 Protein sequence-specific oligonucleotide sequences Withdrawn EP0549692A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58676990A 1990-09-21 1990-09-21
US586769 1990-09-21

Publications (2)

Publication Number Publication Date
EP0549692A1 true EP0549692A1 (fr) 1993-07-07
EP0549692A4 EP0549692A4 (en) 1993-08-25

Family

ID=24347046

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19910917153 Withdrawn EP0549692A4 (en) 1990-09-21 1991-09-18 Protein sequence-specific oligonucleotide sequences

Country Status (4)

Country Link
EP (1) EP0549692A4 (fr)
JP (1) JPH06503715A (fr)
AU (1) AU8547691A (fr)
WO (1) WO1992005285A1 (fr)

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US5668264A (en) * 1990-06-11 1997-09-16 Nexstar Pharmaceuticals, Inc. High affinity PDGF nucleic acid ligands
US5707796A (en) * 1990-06-11 1998-01-13 Nexstar Pharmaceuticals, Inc. Method for selecting nucleic acids on the basis of structure
US5789163A (en) * 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Enzyme linked oligonucleotide assays (ELONAS)
US6395888B1 (en) 1996-02-01 2002-05-28 Gilead Sciences, Inc. High affinity nucleic acid ligands of complement system proteins
US5674685A (en) * 1990-06-11 1997-10-07 Nexstar Pharmaceuticals, Inc. High affinity PDGF nucleic acid ligands
US5840867A (en) * 1991-02-21 1998-11-24 Gilead Sciences, Inc. Aptamer analogs specific for biomolecules
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US5747253A (en) * 1991-08-23 1998-05-05 Isis Pharmaceuticals, Inc. Combinatorial oligomer immunoabsorbant screening assay for transcription factors and other biomolecule binding
JPH09506629A (ja) * 1993-12-17 1997-06-30 キュビッチョッティ，ロジャー・エス ヌクレオチドに支配された生体分子および多分子薬物の集合並びに装置
US5723594A (en) * 1995-06-07 1998-03-03 Nexstar Pharmaceuticals, Inc. High affinity PDGF nucleic acid ligands
US6699843B2 (en) 1995-06-07 2004-03-02 Gilead Sciences, Inc. Method for treatment of tumors using nucleic acid ligands to PDGF
JP2004500109A (ja) 2000-03-22 2004-01-08 クァンタム・ドット・コーポレイション ビーズベースの核酸アッセイにおける半導体ナノクリスタルの使用方法
US20050059031A1 (en) 2000-10-06 2005-03-17 Quantum Dot Corporation Method for enhancing transport of semiconductor nanocrystals across biological membranes
EP2085781B2 (fr) 2000-10-06 2020-03-11 Life Technologies Corporation Cellules dotées d'une signature spectrale et leurs procédés de préparation et leur utilisation
KR20030033007A (ko) 2001-05-31 2003-04-26 코울터 파머수티컬, 인코포레이티드 세포독소, 약물전구체, 링커 및 이에 유용한 안정화제
US8030465B2 (en) 2001-06-29 2011-10-04 Medimolecular Pty Ltd Nucleic acid ligands to complex targets
JP2005500058A (ja) 2001-08-17 2005-01-06 ネオーズ テクノロジーズ， インコーポレイテッド シアリル化したオリゴサッカリドの化学的酵素的合成
US20040023415A1 (en) 2002-03-05 2004-02-05 Konstantin Sokolov Biospecific contrast agents
WO2003078623A1 (fr) * 2002-03-19 2003-09-25 Fujitsu Limited Molecule fonctionnelle et son procede de production
US20040110235A1 (en) 2002-07-25 2004-06-10 David Epstein Regulated aptamer therapeutics
US9303262B2 (en) 2002-09-17 2016-04-05 Archemix Llc Methods for identifying aptamer regulators
US8853376B2 (en) 2002-11-21 2014-10-07 Archemix Llc Stabilized aptamers to platelet derived growth factor and their use as oncology therapeutics
US10100316B2 (en) 2002-11-21 2018-10-16 Archemix Llc Aptamers comprising CPG motifs
US8017755B2 (en) 2003-05-23 2011-09-13 President And Fellows Of Harvard College RNA-based transcriptional regulators
US7803624B2 (en) 2003-09-30 2010-09-28 Cytyc Corporation Automated cytological sample classification
US7803931B2 (en) 2004-02-12 2010-09-28 Archemix Corp. Aptamer therapeutics useful in the treatment of complement-related disorders
DK2860251T3 (en) 2004-02-12 2018-06-06 Archemix Llc APTAPMER PHARMACEUTICALS USEFUL IN TREATMENT OF COMPLEMENT-RELATED DISEASES
US7579450B2 (en) 2004-04-26 2009-08-25 Archemix Corp. Nucleic acid ligands specific to immunoglobulin E and their use as atopic disease therapeutics
US7659091B2 (en) 2004-09-21 2010-02-09 Nourheart, Inc. Diagnostic marker
US7662571B2 (en) 2005-07-14 2010-02-16 Nourheart Inc. Mitochondrial markers of ischemia
BRPI0617546A2 (pt) 2005-09-26 2011-07-26 Medarex Inc conjugado de fÁrmaco-anticorpo, formulaÇço farmacÊutica, mÉtodo para matar uma cÉlula de tumor, mÉtodo para retardar ou interromper o crescimento de um tumor em um sujeito mamÍfero e composto
US20080008694A1 (en) 2006-07-05 2008-01-10 Elgebaly Salwa A Methods to prevent and treat diseases
EP2268664B1 (fr) 2007-12-03 2017-05-24 The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services Compositions doc1 et méthodes de traitement du cancer
US20110229498A1 (en) 2008-05-08 2011-09-22 The Johns Hopkins University Compositions and methods for modulating an immune response
US20100291706A1 (en) 2009-05-15 2010-11-18 Millipore Corporation Dye conjugates and methods of use
WO2013142255A2 (fr) 2012-03-22 2013-09-26 University Of Miami Agents de liaison multi-spécifiques
DE102014114834A1 (de) 2014-10-13 2016-04-14 Centrum Für Angewandte Nanotechnologie (Can) Gmbh Nanopartikel enthaltende Polymermizellen in nicht-wässriger Lösung, Methoden zu ihrer Herstellung und ihrer Anwendung

Citations (3)

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WO1987004170A1 (fr) * 1986-01-09 1987-07-16 Massachusetts Institute Of Technology Facteurs nucleaires associes a la regulation de la transcription
US4772691A (en) * 1985-06-05 1988-09-20 The Medical College Of Wisconsin, Inc. Chemically cleavable nucleotides
WO1991019813A1 (fr) * 1990-06-11 1991-12-26 The University Of Colorado Foundation, Inc. Ligands d'acide nucleique

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4772691A (en) * 1985-06-05 1988-09-20 The Medical College Of Wisconsin, Inc. Chemically cleavable nucleotides
WO1987004170A1 (fr) * 1986-01-09 1987-07-16 Massachusetts Institute Of Technology Facteurs nucleaires associes a la regulation de la transcription
WO1991019813A1 (fr) * 1990-06-11 1991-12-26 The University Of Colorado Foundation, Inc. Ligands d'acide nucleique

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
NUCLEIC ACIDS RESEARCH. vol. 18, no. 11, 11 June 1990, ARLINGTON, VIRGINIA US pages 3203 - 3209 H.-J. THIESEN ET AL. *
SCIENCE vol. 250, 23 November 1990, LANCASTER, PA US pages 1104 - 1110 T.K. BLACKWELL ET AL. *
See also references of WO9205285A1 *

Also Published As

Publication number Publication date
EP0549692A4 (en) 1993-08-25
WO1992005285A1 (fr) 1992-04-02
AU8547691A (en) 1992-04-15
JPH06503715A (ja) 1994-04-28

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