EP0496784B1 - Pipette - Google Patents

Pipette Download PDF

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Publication number
EP0496784B1
EP0496784B1 EP90915595A EP90915595A EP0496784B1 EP 0496784 B1 EP0496784 B1 EP 0496784B1 EP 90915595 A EP90915595 A EP 90915595A EP 90915595 A EP90915595 A EP 90915595A EP 0496784 B1 EP0496784 B1 EP 0496784B1
Authority
EP
European Patent Office
Prior art keywords
pipetter
spindle
piston
adjusting screw
stripper
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP90915595A
Other languages
German (de)
English (en)
Other versions
EP0496784A1 (fr
EP0496784A4 (en
Inventor
Anthony M. Labriola
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Costar Corp
Original Assignee
Costar Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Costar Corp filed Critical Costar Corp
Publication of EP0496784A1 publication Critical patent/EP0496784A1/fr
Publication of EP0496784A4 publication Critical patent/EP0496784A4/en
Application granted granted Critical
Publication of EP0496784B1 publication Critical patent/EP0496784B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops

Definitions

  • This invention relates to pipetters used to transfer fluid from one location to another and more particularly comprises a new and improved manually operated, hand-held pipetter.
  • the principle object of the present invention is to devise such a device which is free of expensive specially machined metal parts and which may be easily assembled and disassembled so as to reduce the manufacturing costs of the device and enable the user to assemble and disassemble it conveniently for cleaning and autoclaving.
  • Another important object of the present invention is to provide a pipetter which may easily and conveniently be held in one hand and which may be adjusted, locked, and actuated by the hand which holds it.
  • Another important object of the present invention is to provide a variable volume pipetter which may be easily and conveniently locked to repeatedly transfer a selected volume and which will not thereafter be accidentally unlocked so as to allow the volume to be altered. Consequently, the device provides a pipetter with reliable repeatability.
  • Another important object of the present invention is to provide a vernier scale in the volume adjustment assembly, which is convenient to use and easy to read and which does not interfere with the use of the device.
  • the pipetter of the present invention comprises the features as delineated in claim 1. Preferred embodiments are delineated in dependent claims 2-14.
  • the pipetter of the present invention has a generally tubular body 20 carrying a cap 22 at its upper end 24 and a tip holder 26 at its bottom end (see FIGS. 1 and 2).
  • the body 20 houses both the mechanism for performing the pipetting action and the stripping mechanism for removing a disposable tip T from the holder.
  • the orientation of the parts of the pipetter are described with respect to the normal, generally vertical position of the instrument in use.
  • the cap 22 is ultrasonically welded or otherwise permanently secured to the body 20 and has a curved flange 28 which normally engages the index finger of the user's hand when the instrument is held in the operative position with the operator's thumb positioned to actuate either of the two pistons in the device, which are described more fully below.
  • the normal gripping position of the pipetter is suggested in the drawings in FIG. 1.
  • the attachment between the cap and the body is facilitated by the cap collar 30 which registers with the upper end 24 of the body 20 (see FIG. 4).
  • the tip holder 26 at its upper end 34 has an enlarged diameter, and at its extreme upper end carries an outwardly extending flange 36 which is engaged by the lip 38 on the lower end of locknut 32.
  • the cylindrical chamber 40 within the top end 34 of holder 26 carries an O-ring 42 held in place by an O-ring retainer 44 also disposed in the enlarged top end 34.
  • the O-ring retainer 44 has an outwardly extending flange 46 at its top, which abuts against the flange 36 of the tip holder.
  • the locknut 32 retains the subassembly comprising the O-ring retainer 44 and tip holder 26 on adjusting screw 48 that forms part of the metering assembly that is described in detail below.
  • a piston 50 and guide rod 52 (hereinafter sometimes collectively called the pipetter piston 53) are coupled together at 54 and move reciprocally together within the body 20 and tip holder 26.
  • the guide rod 52 at its upper end carries a stem 56 which, in turn, carries a plunger button 58 (see FIGS. 4 and 12).
  • the plunger button 58 is positioned to be engaged by the thumb of the hand as suggested in FIG. 1 so as to move the pipetter piston 53 axially and downwardly in the body 20 and tip holder 26 when fluid is to be expelled from the cylindrical passage 60 of the tip holder 26 and the axial passage 61 in the disposable tip T.
  • the length of the vertical stroke of the pipetter piston 53 is controlled by two separate stops mounted in the body 20.
  • the upper limit of the stroke of the pipetter piston is established by the adjustable stop 70 defined by the flange 72 of a spindle cap 74 which in turn is carried by a spindle 76.
  • the spindle 76 is threaded onto the adjusting screw 48 and moves axially on it when the spindle is rotated.
  • the second stop 80 is established by a washer 82 which engages the shoulder 112 of the piston assembly 53.
  • the spindle cap 74 and spindle 76 are separately fabricated.
  • the spindle 76 at its upper end 84 has inner and outer collars 86 and 88 which define an annular space 90 between them into which the collar 92 on the lower end of the spindle cap 74 extends (see FIG. 5).
  • the two parts may be ultrasonically welded together or otherwise secured together so that they are permanently fixed to one another and move as a unit on the adjusting screw 48.
  • the flange 72 of the spindle cap 74 lies above the shoulder 94 of the guide rod 52 and is provided with a small opening 96 at its center through which the stem 56 extends (see FIG. 4).
  • the upward travel of the pipetter piston 53 is limited by engagement of the shoulder 94 on the guide rod 52 with the flange 72.
  • the position of the flange may be varied by rotating spindle 76 on the adjusting screw 48.
  • the spindle 76 may be viewed within the body 20 of the pipetter through a window 100 (see FIG. 2), and the spindle carries a ring 102 with a vernier scale which cooperates with a scale 104 in the window 100.
  • These calibrations are a measure of the volume of fluid to be drawn into the passage 61 in the tip T when the pipetter piston 53 is retracted, as is made clear below in connection with the description of the operation of the instrument.
  • the lower stop 80 disposed within the axial chamber 110 in adjusting screw 48 cooperates with the shoulder 112 formed in the guide rod 52 and which faces downwardly in the pipetter body 20.
  • the washer 82 that comprises the stop is yieldably held in place by a blow-out spring 114 which surrounds the reduced diameter section 116 of the guide rod extending downwardly from the shoulder 112 to the coupling 54 joining the lower end of the guide rod 52 and the piston 50.
  • the blow-out spring 114 bears against the upper face of a spring retainer 120 also mounted in the chamber 110 in adjusting screw 48.
  • the lower stop 80 is yieldable, as the washer 82 may be moved downwardly against the bias of the spring 114 when engaged by the shoulder 112 as the spring 114 compresses.
  • the spring retainer 120 is described in further detail below in connection with the assembling and disassembling of the pipetter.
  • a second coil spring 122 surrounds the piston 50 and extends between the coupling 54 and a seat 126 formed in the lower end of the O-ring retainer 44 (see FIG. 5).
  • the spring 122 serves as the main spring to yieldably oppose downward actuation of the pipetter piston 53. Any downward motion of the pipetter piston 53 causes the coil spring 122 to compress (see FIG. 10), and whenever the actuating button 58 is released, the pipetter piston returns to the upper position shown in FIG. 2. During the initial movement of the pipetter piston 53, only the spring 122 is compressed, and the resistance to actuation of the pipetter piston is essentially uniform throughout the major portion of the stroke established by the stops 70 and 80 and shoulders 94 and 112.
  • a stripper assembly 150 is in part mounted within the body 20 and in part lies below the body and surrounds the tip holder 26.
  • the stripper assembly 150 includes a stem 152 mounted for reciprocal motion within the housing 20 along a path parallel to the axis of the pipetter piston 53 (see FIG. 4).
  • the rod 152 carries an actuating button 154 at its upper end 156, which may be conveniently actuated by the thumb of the hand which grips the body 20, as is evident in FIG. 1.
  • the rod 152 is supported in an opening 158 in flange 160 formed as an integral part of the body 20.
  • a compression spring 162 surrounds the upper portion of the rod 152 between the flange 160 and the lower end 164 of actuating button 154 and urges the rod upwardly in the body.
  • the upward travel of the rod 152 is limited by the shoulder 166 formed on the rod and which engages the flange 160.
  • the downward travel of the rod 150 is limited by engagement of the flange 170 of the actuating button 154 with the edge 172 of the body 20.
  • the lower end 176 of the rod 152 has a threaded stem 178 of reduced diameter, which extends through the flange 179 of a tubular stripper 181. The two are held in assembled relationship by the retaining nut 180.
  • the stripper 181 is tapered slightly in a downward direction so that its lower end 182 has an inner diameter which is only slightly greater than the outer diameter of the tip holder 26.
  • the inner diameter of the stripper 181 increases in an upward direction and is significantly enlarged at its upper end 184 so as to surround the locknut 32.
  • the strength of the stripper 181 at its upper end is enhanced by the rib 188 connected to the flange 179 at the location where the threaded stem 178 of the rod 152 extends through the flange.
  • the spindle 76 on the adjusting screw 48 varies the position of the upper stop 70 which limits the extent that the pipetter piston 53 may be retracted into the body 20. It is essential for accurate repeatability of the pipetter that any particular setting of the stop 70 be fixed when a series of liquid transfers is to be made by the device.
  • the spindle may be locked against rotation on the adjusting screw by means of the lock slide 200 (see FIGS. 4, 6 and 7).
  • the lock slide 200 mounted in body 20 is moved translationally in the body by means of a finger grip 202 mounted in slot 204 in the body wall 206.
  • the finger grip has an upwardly extending arm 208 which extends along the outer surface of the adjusting screw 48 and into the lower end of the cylindrical spindle 76.
  • the spindle 76 overlaps the upper end 210 of the arm 208 even in the uppermost position of the spindle as shown in FIG. 4.
  • a ramp 212 is formed on the outer surface of the adjusting screw 48, which cooperates with a cam surface 214 on the inner face of the upper end 210 of arm 208.
  • the cam surface 214 rides upwardly on the ramp 212 causing the end 210 of the arm to bear against and bind on the inner surface of the spindle 76.
  • the friction contact between the arm 208 and the inner surface of the spindle prevents the spindle from rotating and, therefore, the stop 70 remains in a fixed position to insure accurate, repetitive metering of the fluid. The spindle will not be moved again until the lock slide 200 is released.
  • the adjusting screw 48 is split so as to divide its lower end into two finger-like portions 219.
  • Each finger portion carries an ear 220 on its outer surface that is sized to register with the openings 222 in the body wall 206.
  • the finger portions 219 are squeezed together as in FIG. 9 so that they allow the ears 220 to clear the lower edge 223 of the body and snap into the openings 222.
  • the spring retainer 120 is inserted into the adjusting screw to prevent its finger portions 219 from collapsing toward one another and dislodging the ears 220 from the openings 222. It will be noted particularly in FIG.
  • the retainer 120 may be pulled from the adjusting screw, and the adjusting screw may then be removed from the body 20.
  • the retainer 120 also locks in the blow-out spring 114 that biases the piston 53 upwardly in the assembly.
  • the retainer 120 may have projections 120' at its upper end that register with slots in the adjusting screw to form a bayonet connection between the two, to prevent the retainer from accidentally falling from the assembly (see FIGS. 4 and 12).
  • a portion of the spindle 76 is clearly visible through the window 100 in the body.
  • the portion 102 viewable through the window carries a vernier scale which cooperates with the linear scale 104 on the window 100 so as to subdivide each unit of the linear scale into ten parts.
  • the units are a function of the pitch of the adjusting screw and spindle, the diameter of the pipetter piston 53, etc.
  • the pipetter of the present invention operates as follows: Assume a reservoir of fluid to be transferred and a plurality of wells to receive the transferred liquid are provided, and a selected volume to be transferred from the reservoir to each of the wells is known. The operator rotates the exposed portion of the spindle so as to set it to the selected position on the scale visible through the window. Assume also that a rack of disposable pipetter tips T is provided which enables the tip holder 26 to be inserted in the appropriate end of the disposable tip and frictionally engage it.
  • the tip T With the pipetter piston 53 in that position, the tip T is placed in the reservoir with its lower end submerged in the liquid, and the button 58 is thereupon released allowing the pipetter piston 53 to rise under the influence of the spring 114 until the shoulder 94 of the piston engages the flange 72 as shown in FIG. 14. This action causes liquid to be drawn into the tip T in the volume set on the vernier scale.
  • the pipetter is next positioned with the tip T aligned with the open end of the well W to which the liquid is to be transferred, and the operator again depresses the button 58 causing the pipetter piston to move downwardly.
  • the pressure in the passage 60 in the tip holder and the passage 61 in the tip T causes the liquid in tip T to be discharged into the well.
  • the operator need only press the button 154 and move the rod 152 against the bias of spring 162 until the flange 170 of the button engages the end 172 of the body 20. This action will cause the stripper 181 to move downwardly on the tip holder and cause the lower end 182 of the stripper to engage the tip T and force it off the holder 26 as suggested in FIG. 17.
  • the pipetter of the present invention may very quickly and easily be adjusted to vary the volume of liquid drawn into the device upon actuation of the button 58.
  • the adjustment is made simply by turning the spindle which is exposed through the window 100.
  • the top stop 70 may be positioned so as to cause the pipetter piston to draw in the desired volume of liquid when the pipetter piston is depressed and then released.
  • the vernier scale visible in the window enables the user to quickly set the stop to provide the desired metering. While the spindle is very easily turned, it may quickly and firmly be locked in any set position by means of the lock slide 200.
  • the arm 208 By moving the slide upwardly toward the top end of the body in the slot provided in the outer wall, the arm 208 will wedge underneath the lower end of the spindle 76 and prevent it from rotating and changing the position of stop 70. This will ensure accurate repeatability of the device as it is used over and over again to transfer like quantities of liquid.
  • the lock is very effective and easy to operate, it may be readily disengaged merely by sliding the lock slide downwardly by means of the finger grip 202 so that the arm 208 releases the spindle.
  • Another advantage of the present invention is the elimination in the device of screw machined parts. All but a few of the parts of the device may be injection molded of a suitable plastic material. This mode of construction is reflected in reduced manufacturing costs for the device. Manufacturing costs are also minimized by the presence of only a few ultrasonically welded parts. In the construction of the pipetter described, only two ultrasonic welds are included, namely, at the attachment of the button 58 to the pipetter shaft 53 and at the connection of the stop 70 to the spindle 76.
  • Another advantage of the present invention is the ease with which the device may be taken apart for cleaning or sterilization.
  • the stripper may be removed from the assembly so as to expose the full length of the tip holder 26 and the coupling which joins the tip holder to the adjusting screw 48 and body 20. More particularly, removal of the stripper exposes the locknut 32 which may then be turned so as to free the flange 36 of the tip holder. And with the tip holder removed, the retainer nut 44 may be removed, which will allow the piston 50 to be withdrawn.
  • blow-out spring retainer 120 from the adjusting screw 48 and the plunger button 58 from the stem 56 of the guide rod 52
  • the blow-out spring 114, washer 82 and guide rod 52 may also be withdrawn from the body 20 through its lower end.
  • the device may be further disassembled by squeezing the fingers 219 of the split adjusting screw 48 together to release the ears 220 from the openings 222.

Abstract

Pipette portative actionnée manuellement, ayant un volume variable régulé par une tige rotative (76) et une vis de réglage (48), lesquelles régulent la position d'une butée (70), laquelle limite l'extrémité supérieure de la course du piston (53) de la pipette. L'extrémité inférieure de la course est régulée par une butée (80) mobile, poussée jusqu'à une première position par un ressort (114) de souffle, lequel peut être maîtrisé par une force supplémentaire appliquée au piston (53) de la pipette lorsque le contact se produit avec la butée (80) inférieure. Ladite pipette comprend également un éjecteur (181), lequel entoure le support (26) de la pointe et lequel est actionné par une tige (152) mobile dans le corps (20) de la pipette, le long d'un chemin parallèle au chemin du piston (53) de la pipette. Le piston (53) de la pipette et la tige (181) de l'éjecteur sont commandés par des actuateurs (58, 154) séparés, permettant un contact facile avec le pouce de la main maintenant le corps (20) du dispositif.

Claims (14)

  1. Pipette comprenant un corps tubulaire allongé ayant un axe et un cylindre délimitant un volume de décharge, une vis tubulaire de réglage montée coaxialement à l'intérieur de et fixée étroitement au corps, la vis de réglage comportant sur une partie un filetage, une broche tubulaire pouvant être entraînée en rotation à la main, s'ajustant sur la vis de réglage et ayant un taraudage qui correspond au filetage de la vis de réglage de façon que la broche puisse être ajustée axialement par rapport à la vis de réglage, un piston disposé dans la vis de réglage et la broche à l'intérieur du cylindre, le piston étant déplaçable axialement vers la broche pendant une course d'admission et déplaçable axialement vers la vis de réglage pendant une course de décharge, ainsi qu'une butée reliée à la broche pour limiter le mouvement axial du piston pendant la course d'admission.
  2. Pipette selon la revendication 1, comprenant par ailleurs une graduation axiale sur une partie non filetée de la vis de réglage et une graduation circulaire sur une extrémité de la broche qui s'ajuste sur la vis de réglage, la graduation circulaire coopérant avec la graduation axiale de manière que la broche soit réglable de manière répétitive par rapport à la vis de réglage en incréments de fractions de filet de vis.
  3. Pipette selon la revendication 2, dans laquelle le corps comporte une fenêtre de manière que la graduation axiale et la graduation circulaire soient visibles.
  4. Pipette selon la revendication 3, dans laquelle la broche peut être atteinte par la fenêtre pour pouvoir être entraînée en rotation à la main.
  5. Pipette selon la revendication 1, dans laquelle le piston comporte un épaulement radial situé dans la broche et la butée consiste en un capuchon relié à une extrémité de la broche, le capuchon étant traversé par un trou et le piston passant par le trou de façon que l'épaulement entre en contact avec le capuchon et limite le mouvement du piston pendant la course d'admission.
  6. Pipette selon la revendication 1, comprenant par ailleurs un ressort hélicoïdal de compression placé sur le piston et situé entre le piston et la vis de réglage, le ressort repoussant le piston vers le broche.
  7. Pipette selon la revendication 6, dans laquelle une seconde butée est montée dans le corps sur le trajet du piston de la pipette afin de résister au déplacement du piston en direction vers le bas au-delà d'une position présélectionnée et un second ressort est monté dans le corps de manière qu'il s'applique contre la seconde butée pour retenir, tout en s'affaissant, la seconde butée à la position présélectionnée, mais en permettant au piston de se déplacer au-delà de cette position.
  8. Pipette selon la revendication 1, dans laquelle la vis de réglage est montée de manière amovible sur le corps au moyen de plusieurs pattes flexibles, lesdites pattes se logeant dans des rainures du corps.
  9. Pipette selon la revendication 1, dans laquelle le corps comporte une extrémité supérieure et une extrémité inférieure et dans laquelle la pipette comprend par ailleurs un support d'embout fixé à l'extrémité inférieure du corps et comportant intérieurement un passage qui est aligné axialement sur l'axe du corps, le support d'embout comportant une extrémité inférieure destinée à être emboîtée avec et à supporter des embouts amovibles, un éjecteur allongé entourant une partie du support d'embout et étant mobile axialement sur ce support pour enlever un embout monté sur l'extrémité inférieure du support d'embout et une tige d'actionnement montée dans le corps et fixée à l'éjecteur pour imposer un mouvement à l'éjecteur parallèlement à l'axe du corps de façon à actionner l'éjecteur.
  10. Pipette selon la revendication 9, dans laquelle la tige d'actionnement est reliée de manière amovible à l'éjecteur à l'extérieur du corps.
  11. Pipette selon la revendication 9, dans laquelle un raccord relie de manière amovible le support d'embout et la vis de réglage, l'éjecteur enveloppant le raccord.
  12. Pipette selon la revendication 1, dans laquelle un moyen s'actionnant à la main est monté dans le corps pour s'enclencher de manière libérable avec la broche afin d'empêcher la broche de tourner.
  13. Pipette selon la revendication 12, dans laquelle le moyen s'enclenchant sur la broche est un patin qui s'applique avec frottement contre la broche pour l'empêcher de tourner.
  14. Pipette selon la revendication 1, dans laquelle la vis de réglage est réalisée de manière à être compressible radialement pour être introduite dans le corps et des moyens sont montés de manière amovible dans la vis de réglage pour empêcher la vis d'être comprimée radialement après que la vis de réglage a été introduite dans le corps.
EP90915595A 1989-10-20 1990-10-10 Pipette Expired - Lifetime EP0496784B1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US42440889A 1989-10-20 1989-10-20
US424408 1989-10-20
PCT/US1990/005800 WO1991005609A1 (fr) 1989-10-20 1990-10-10 Pipette

Publications (3)

Publication Number Publication Date
EP0496784A1 EP0496784A1 (fr) 1992-08-05
EP0496784A4 EP0496784A4 (en) 1992-09-09
EP0496784B1 true EP0496784B1 (fr) 1994-04-27

Family

ID=23682526

Family Applications (1)

Application Number Title Priority Date Filing Date
EP90915595A Expired - Lifetime EP0496784B1 (fr) 1989-10-20 1990-10-10 Pipette

Country Status (4)

Country Link
EP (1) EP0496784B1 (fr)
JP (1) JPH05501222A (fr)
DE (1) DE69008540T2 (fr)
WO (1) WO1991005609A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2826722A1 (fr) 2013-07-19 2015-01-21 Mettler-Toledo AG Appareil de dosage manuel pour des poudres et des pâtes

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US5435355A (en) * 1992-10-09 1995-07-25 Xiao; Yong Conical floating type fuel filling device
DE9415847U1 (de) * 1994-09-30 1994-12-08 Ritter Ralf Repetierpipette
US5983733A (en) * 1996-11-15 1999-11-16 Hamilton Company Manual pipette
JP3473409B2 (ja) * 1998-06-11 2003-12-02 松下電器産業株式会社 分注装置
US6428750B1 (en) * 2000-02-17 2002-08-06 Rainin Instrument, Llc Volume adjustable manual pipette with quick set volume adjustment
FI20002241A0 (fi) * 2000-10-11 2000-10-11 Labsystems Oy Säädettävä pipetti
FIU20010274U0 (fi) * 2001-07-06 2001-07-06 Biohit Oyj Imulaite
ATE480330T1 (de) 2001-10-16 2010-09-15 Matrix Technologies Corp Hand-pipettiervorrichtung
DE102004003434B4 (de) * 2004-01-21 2006-06-08 Eppendorf Ag Pipettiervorrichtung mit einer Verdrängungseinrichtung und einer damit lösbar verbundenen Antriebseinrichtung
FI20050340A0 (fi) 2005-04-01 2005-04-01 Thermo Electron Oy Kalibroitava pipetti
DE102005041183B3 (de) * 2005-08-31 2007-01-04 Eppendorf Ag Pipettiervorrichtung
JP5657861B2 (ja) * 2008-12-30 2015-01-21 光陽化成有限会社 マイクロピペット装置
WO2016002019A1 (fr) * 2014-07-01 2016-01-07 株式会社安川電機 Dispositif de transport de liquide et procédé de transport de liquide
EP3112026B1 (fr) 2015-06-30 2018-03-14 Sartorius Biohit Liquid Handling Oy Pipette avec un mécanisme de retrait de pointe, procédé pour retirer une pointe et procédé de pipettage
JP7315625B2 (ja) * 2020-07-14 2023-07-26 エッペンドルフ エスイー 一体型プランジャを備えたピペット・チップとともに使用するためのピペット
FR3128889B1 (fr) * 2021-11-09 2023-09-29 Gilson Sas Dispositif de prelevement a deplacement positif, conçu ameliorer la prehension du piston d’un systeme piston-capillaire

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AU6276773A (en) * 1972-11-21 1975-05-22 Excalibur Lab Pty Ltd Pipette
US4054062A (en) * 1976-05-03 1977-10-18 Oxford Laboratories Inc. Hand-held micropipettor with improved accuracy of liquid volumes transferred
FI57540C (fi) * 1977-02-17 1980-09-10 Suovaniemi Finnpipette Anordning foer att loesgoera och avlaegsna en engaongsspets pao en pipett
FI57543C (fi) * 1978-06-02 1980-09-10 Suovaniemi Finnpipette Volymreglerbar pipett
FI56937C (fi) * 1978-08-04 1980-05-12 Suovaniemi Finnpipette Anordning foer att avlaegsna en engaongsspetsbehaollare fraon en pipett
JP2554666B2 (ja) * 1987-09-18 1996-11-13 株式会社ニチリョー 可変式ピペット

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2826722A1 (fr) 2013-07-19 2015-01-21 Mettler-Toledo AG Appareil de dosage manuel pour des poudres et des pâtes
US9517849B2 (en) 2013-07-19 2016-12-13 Mettler-Toledo Gmbh Handheld dosage-dispensing instrument for powderous or pasteous dosage material

Also Published As

Publication number Publication date
EP0496784A1 (fr) 1992-08-05
WO1991005609A1 (fr) 1991-05-02
JPH05501222A (ja) 1993-03-11
DE69008540D1 (en) 1994-06-01
DE69008540T2 (de) 1994-12-15
EP0496784A4 (en) 1992-09-09

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