EP0496784B1 - Pipetter - Google Patents
Pipetter Download PDFInfo
- Publication number
- EP0496784B1 EP0496784B1 EP90915595A EP90915595A EP0496784B1 EP 0496784 B1 EP0496784 B1 EP 0496784B1 EP 90915595 A EP90915595 A EP 90915595A EP 90915595 A EP90915595 A EP 90915595A EP 0496784 B1 EP0496784 B1 EP 0496784B1
- Authority
- EP
- European Patent Office
- Prior art keywords
- pipetter
- spindle
- piston
- adjusting screw
- stripper
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops
Definitions
- This invention relates to pipetters used to transfer fluid from one location to another and more particularly comprises a new and improved manually operated, hand-held pipetter.
- the principle object of the present invention is to devise such a device which is free of expensive specially machined metal parts and which may be easily assembled and disassembled so as to reduce the manufacturing costs of the device and enable the user to assemble and disassemble it conveniently for cleaning and autoclaving.
- Another important object of the present invention is to provide a pipetter which may easily and conveniently be held in one hand and which may be adjusted, locked, and actuated by the hand which holds it.
- Another important object of the present invention is to provide a variable volume pipetter which may be easily and conveniently locked to repeatedly transfer a selected volume and which will not thereafter be accidentally unlocked so as to allow the volume to be altered. Consequently, the device provides a pipetter with reliable repeatability.
- Another important object of the present invention is to provide a vernier scale in the volume adjustment assembly, which is convenient to use and easy to read and which does not interfere with the use of the device.
- the pipetter of the present invention comprises the features as delineated in claim 1. Preferred embodiments are delineated in dependent claims 2-14.
- the pipetter of the present invention has a generally tubular body 20 carrying a cap 22 at its upper end 24 and a tip holder 26 at its bottom end (see FIGS. 1 and 2).
- the body 20 houses both the mechanism for performing the pipetting action and the stripping mechanism for removing a disposable tip T from the holder.
- the orientation of the parts of the pipetter are described with respect to the normal, generally vertical position of the instrument in use.
- the cap 22 is ultrasonically welded or otherwise permanently secured to the body 20 and has a curved flange 28 which normally engages the index finger of the user's hand when the instrument is held in the operative position with the operator's thumb positioned to actuate either of the two pistons in the device, which are described more fully below.
- the normal gripping position of the pipetter is suggested in the drawings in FIG. 1.
- the attachment between the cap and the body is facilitated by the cap collar 30 which registers with the upper end 24 of the body 20 (see FIG. 4).
- the tip holder 26 at its upper end 34 has an enlarged diameter, and at its extreme upper end carries an outwardly extending flange 36 which is engaged by the lip 38 on the lower end of locknut 32.
- the cylindrical chamber 40 within the top end 34 of holder 26 carries an O-ring 42 held in place by an O-ring retainer 44 also disposed in the enlarged top end 34.
- the O-ring retainer 44 has an outwardly extending flange 46 at its top, which abuts against the flange 36 of the tip holder.
- the locknut 32 retains the subassembly comprising the O-ring retainer 44 and tip holder 26 on adjusting screw 48 that forms part of the metering assembly that is described in detail below.
- a piston 50 and guide rod 52 (hereinafter sometimes collectively called the pipetter piston 53) are coupled together at 54 and move reciprocally together within the body 20 and tip holder 26.
- the guide rod 52 at its upper end carries a stem 56 which, in turn, carries a plunger button 58 (see FIGS. 4 and 12).
- the plunger button 58 is positioned to be engaged by the thumb of the hand as suggested in FIG. 1 so as to move the pipetter piston 53 axially and downwardly in the body 20 and tip holder 26 when fluid is to be expelled from the cylindrical passage 60 of the tip holder 26 and the axial passage 61 in the disposable tip T.
- the length of the vertical stroke of the pipetter piston 53 is controlled by two separate stops mounted in the body 20.
- the upper limit of the stroke of the pipetter piston is established by the adjustable stop 70 defined by the flange 72 of a spindle cap 74 which in turn is carried by a spindle 76.
- the spindle 76 is threaded onto the adjusting screw 48 and moves axially on it when the spindle is rotated.
- the second stop 80 is established by a washer 82 which engages the shoulder 112 of the piston assembly 53.
- the spindle cap 74 and spindle 76 are separately fabricated.
- the spindle 76 at its upper end 84 has inner and outer collars 86 and 88 which define an annular space 90 between them into which the collar 92 on the lower end of the spindle cap 74 extends (see FIG. 5).
- the two parts may be ultrasonically welded together or otherwise secured together so that they are permanently fixed to one another and move as a unit on the adjusting screw 48.
- the flange 72 of the spindle cap 74 lies above the shoulder 94 of the guide rod 52 and is provided with a small opening 96 at its center through which the stem 56 extends (see FIG. 4).
- the upward travel of the pipetter piston 53 is limited by engagement of the shoulder 94 on the guide rod 52 with the flange 72.
- the position of the flange may be varied by rotating spindle 76 on the adjusting screw 48.
- the spindle 76 may be viewed within the body 20 of the pipetter through a window 100 (see FIG. 2), and the spindle carries a ring 102 with a vernier scale which cooperates with a scale 104 in the window 100.
- These calibrations are a measure of the volume of fluid to be drawn into the passage 61 in the tip T when the pipetter piston 53 is retracted, as is made clear below in connection with the description of the operation of the instrument.
- the lower stop 80 disposed within the axial chamber 110 in adjusting screw 48 cooperates with the shoulder 112 formed in the guide rod 52 and which faces downwardly in the pipetter body 20.
- the washer 82 that comprises the stop is yieldably held in place by a blow-out spring 114 which surrounds the reduced diameter section 116 of the guide rod extending downwardly from the shoulder 112 to the coupling 54 joining the lower end of the guide rod 52 and the piston 50.
- the blow-out spring 114 bears against the upper face of a spring retainer 120 also mounted in the chamber 110 in adjusting screw 48.
- the lower stop 80 is yieldable, as the washer 82 may be moved downwardly against the bias of the spring 114 when engaged by the shoulder 112 as the spring 114 compresses.
- the spring retainer 120 is described in further detail below in connection with the assembling and disassembling of the pipetter.
- a second coil spring 122 surrounds the piston 50 and extends between the coupling 54 and a seat 126 formed in the lower end of the O-ring retainer 44 (see FIG. 5).
- the spring 122 serves as the main spring to yieldably oppose downward actuation of the pipetter piston 53. Any downward motion of the pipetter piston 53 causes the coil spring 122 to compress (see FIG. 10), and whenever the actuating button 58 is released, the pipetter piston returns to the upper position shown in FIG. 2. During the initial movement of the pipetter piston 53, only the spring 122 is compressed, and the resistance to actuation of the pipetter piston is essentially uniform throughout the major portion of the stroke established by the stops 70 and 80 and shoulders 94 and 112.
- a stripper assembly 150 is in part mounted within the body 20 and in part lies below the body and surrounds the tip holder 26.
- the stripper assembly 150 includes a stem 152 mounted for reciprocal motion within the housing 20 along a path parallel to the axis of the pipetter piston 53 (see FIG. 4).
- the rod 152 carries an actuating button 154 at its upper end 156, which may be conveniently actuated by the thumb of the hand which grips the body 20, as is evident in FIG. 1.
- the rod 152 is supported in an opening 158 in flange 160 formed as an integral part of the body 20.
- a compression spring 162 surrounds the upper portion of the rod 152 between the flange 160 and the lower end 164 of actuating button 154 and urges the rod upwardly in the body.
- the upward travel of the rod 152 is limited by the shoulder 166 formed on the rod and which engages the flange 160.
- the downward travel of the rod 150 is limited by engagement of the flange 170 of the actuating button 154 with the edge 172 of the body 20.
- the lower end 176 of the rod 152 has a threaded stem 178 of reduced diameter, which extends through the flange 179 of a tubular stripper 181. The two are held in assembled relationship by the retaining nut 180.
- the stripper 181 is tapered slightly in a downward direction so that its lower end 182 has an inner diameter which is only slightly greater than the outer diameter of the tip holder 26.
- the inner diameter of the stripper 181 increases in an upward direction and is significantly enlarged at its upper end 184 so as to surround the locknut 32.
- the strength of the stripper 181 at its upper end is enhanced by the rib 188 connected to the flange 179 at the location where the threaded stem 178 of the rod 152 extends through the flange.
- the spindle 76 on the adjusting screw 48 varies the position of the upper stop 70 which limits the extent that the pipetter piston 53 may be retracted into the body 20. It is essential for accurate repeatability of the pipetter that any particular setting of the stop 70 be fixed when a series of liquid transfers is to be made by the device.
- the spindle may be locked against rotation on the adjusting screw by means of the lock slide 200 (see FIGS. 4, 6 and 7).
- the lock slide 200 mounted in body 20 is moved translationally in the body by means of a finger grip 202 mounted in slot 204 in the body wall 206.
- the finger grip has an upwardly extending arm 208 which extends along the outer surface of the adjusting screw 48 and into the lower end of the cylindrical spindle 76.
- the spindle 76 overlaps the upper end 210 of the arm 208 even in the uppermost position of the spindle as shown in FIG. 4.
- a ramp 212 is formed on the outer surface of the adjusting screw 48, which cooperates with a cam surface 214 on the inner face of the upper end 210 of arm 208.
- the cam surface 214 rides upwardly on the ramp 212 causing the end 210 of the arm to bear against and bind on the inner surface of the spindle 76.
- the friction contact between the arm 208 and the inner surface of the spindle prevents the spindle from rotating and, therefore, the stop 70 remains in a fixed position to insure accurate, repetitive metering of the fluid. The spindle will not be moved again until the lock slide 200 is released.
- the adjusting screw 48 is split so as to divide its lower end into two finger-like portions 219.
- Each finger portion carries an ear 220 on its outer surface that is sized to register with the openings 222 in the body wall 206.
- the finger portions 219 are squeezed together as in FIG. 9 so that they allow the ears 220 to clear the lower edge 223 of the body and snap into the openings 222.
- the spring retainer 120 is inserted into the adjusting screw to prevent its finger portions 219 from collapsing toward one another and dislodging the ears 220 from the openings 222. It will be noted particularly in FIG.
- the retainer 120 may be pulled from the adjusting screw, and the adjusting screw may then be removed from the body 20.
- the retainer 120 also locks in the blow-out spring 114 that biases the piston 53 upwardly in the assembly.
- the retainer 120 may have projections 120' at its upper end that register with slots in the adjusting screw to form a bayonet connection between the two, to prevent the retainer from accidentally falling from the assembly (see FIGS. 4 and 12).
- a portion of the spindle 76 is clearly visible through the window 100 in the body.
- the portion 102 viewable through the window carries a vernier scale which cooperates with the linear scale 104 on the window 100 so as to subdivide each unit of the linear scale into ten parts.
- the units are a function of the pitch of the adjusting screw and spindle, the diameter of the pipetter piston 53, etc.
- the pipetter of the present invention operates as follows: Assume a reservoir of fluid to be transferred and a plurality of wells to receive the transferred liquid are provided, and a selected volume to be transferred from the reservoir to each of the wells is known. The operator rotates the exposed portion of the spindle so as to set it to the selected position on the scale visible through the window. Assume also that a rack of disposable pipetter tips T is provided which enables the tip holder 26 to be inserted in the appropriate end of the disposable tip and frictionally engage it.
- the tip T With the pipetter piston 53 in that position, the tip T is placed in the reservoir with its lower end submerged in the liquid, and the button 58 is thereupon released allowing the pipetter piston 53 to rise under the influence of the spring 114 until the shoulder 94 of the piston engages the flange 72 as shown in FIG. 14. This action causes liquid to be drawn into the tip T in the volume set on the vernier scale.
- the pipetter is next positioned with the tip T aligned with the open end of the well W to which the liquid is to be transferred, and the operator again depresses the button 58 causing the pipetter piston to move downwardly.
- the pressure in the passage 60 in the tip holder and the passage 61 in the tip T causes the liquid in tip T to be discharged into the well.
- the operator need only press the button 154 and move the rod 152 against the bias of spring 162 until the flange 170 of the button engages the end 172 of the body 20. This action will cause the stripper 181 to move downwardly on the tip holder and cause the lower end 182 of the stripper to engage the tip T and force it off the holder 26 as suggested in FIG. 17.
- the pipetter of the present invention may very quickly and easily be adjusted to vary the volume of liquid drawn into the device upon actuation of the button 58.
- the adjustment is made simply by turning the spindle which is exposed through the window 100.
- the top stop 70 may be positioned so as to cause the pipetter piston to draw in the desired volume of liquid when the pipetter piston is depressed and then released.
- the vernier scale visible in the window enables the user to quickly set the stop to provide the desired metering. While the spindle is very easily turned, it may quickly and firmly be locked in any set position by means of the lock slide 200.
- the arm 208 By moving the slide upwardly toward the top end of the body in the slot provided in the outer wall, the arm 208 will wedge underneath the lower end of the spindle 76 and prevent it from rotating and changing the position of stop 70. This will ensure accurate repeatability of the device as it is used over and over again to transfer like quantities of liquid.
- the lock is very effective and easy to operate, it may be readily disengaged merely by sliding the lock slide downwardly by means of the finger grip 202 so that the arm 208 releases the spindle.
- Another advantage of the present invention is the elimination in the device of screw machined parts. All but a few of the parts of the device may be injection molded of a suitable plastic material. This mode of construction is reflected in reduced manufacturing costs for the device. Manufacturing costs are also minimized by the presence of only a few ultrasonically welded parts. In the construction of the pipetter described, only two ultrasonic welds are included, namely, at the attachment of the button 58 to the pipetter shaft 53 and at the connection of the stop 70 to the spindle 76.
- Another advantage of the present invention is the ease with which the device may be taken apart for cleaning or sterilization.
- the stripper may be removed from the assembly so as to expose the full length of the tip holder 26 and the coupling which joins the tip holder to the adjusting screw 48 and body 20. More particularly, removal of the stripper exposes the locknut 32 which may then be turned so as to free the flange 36 of the tip holder. And with the tip holder removed, the retainer nut 44 may be removed, which will allow the piston 50 to be withdrawn.
- blow-out spring retainer 120 from the adjusting screw 48 and the plunger button 58 from the stem 56 of the guide rod 52
- the blow-out spring 114, washer 82 and guide rod 52 may also be withdrawn from the body 20 through its lower end.
- the device may be further disassembled by squeezing the fingers 219 of the split adjusting screw 48 together to release the ears 220 from the openings 222.
Abstract
Description
- This invention relates to pipetters used to transfer fluid from one location to another and more particularly comprises a new and improved manually operated, hand-held pipetter.
- There are at the present time many single channeled pipetters on the market. These devices are widely used in clinics and laboratories to transfer liquids in very small volumes from one location to another such as from a reservoir containing larger volumes of the liquid to very small wells and plates in which tests and experiments are conducted.
- The principle object of the present invention is to devise such a device which is free of expensive specially machined metal parts and which may be easily assembled and disassembled so as to reduce the manufacturing costs of the device and enable the user to assemble and disassemble it conveniently for cleaning and autoclaving.
- Another important object of the present invention is to provide a pipetter which may easily and conveniently be held in one hand and which may be adjusted, locked, and actuated by the hand which holds it.
- Another important object of the present invention is to provide a variable volume pipetter which may be easily and conveniently locked to repeatedly transfer a selected volume and which will not thereafter be accidentally unlocked so as to allow the volume to be altered. Consequently, the device provides a pipetter with reliable repeatability.
- Another important object of the present invention is to provide a vernier scale in the volume adjustment assembly, which is convenient to use and easy to read and which does not interfere with the use of the device.
- The pipetter of the present invention comprises the features as delineated in claim 1. Preferred embodiments are delineated in dependent claims 2-14.
- These and other objects and features of the present invention will be better understood and appreciated from the following detailed description of one embodiment thereof, selected for purposes of illustration and shown in the accompanying drawing, in which:
- FIG. 1 is a perspective view of a pipetter constructed in accordance with this invention and shown as it may be held in the hand of a user;
- FIG. 2 is an enlarged front elevation view of the pipetter shown in FIG. 1;
- FIG. 3 is a top view of the pipetter;
- FIG. 4 is a longitudinal cross-sectional view of the pipetter taken along section line 4-4 in FIG. 3;
- FIG. 5 is an enlarged fragmentary cross sectional view of the pipetter taken along section line 5-5 in FIG. 4;
- FIG. 6 is a cross-sectional view of the pipetter taken along section line 6-6 in FIG. 4 and showing the spindle of the device in the locked position;
- FIG. 7 is a cross-sectional view taken along section line 7-7 in FIG. 6 but showing the spindle of the pipetter in the unlocked position;
- FIG. 8 is a cross-sectional view of the assembled pipetter taken along the section line 8-8 in FIG. 4:
- FIG. 9 is a cross-sectional view similar to FIG. 8 but showing the pipetter in the partially assembled form;
- FIGS. 10 and 11 are fragmentary cross-sectional views similar to FIG. 5 but showing the pipetter piston in the partially and fully depressed positions, respectively;
- FIG. 12 is an exploded view of the pipetter showing the major components of the pipetter and suggesting how they are assembled together; and
- FIGS. 13-17 are diagrammatic views showing the sequence of steps through which the pipetter is operated to dispenser metered quantities of liquid and to strip a tip from the tip holder.
- The pipetter of the present invention has a generally
tubular body 20 carrying acap 22 at itsupper end 24 and atip holder 26 at its bottom end (see FIGS. 1 and 2). Thebody 20 houses both the mechanism for performing the pipetting action and the stripping mechanism for removing a disposable tip T from the holder. In this description, the orientation of the parts of the pipetter are described with respect to the normal, generally vertical position of the instrument in use. - The
cap 22 is ultrasonically welded or otherwise permanently secured to thebody 20 and has acurved flange 28 which normally engages the index finger of the user's hand when the instrument is held in the operative position with the operator's thumb positioned to actuate either of the two pistons in the device, which are described more fully below. The normal gripping position of the pipetter is suggested in the drawings in FIG. 1. The attachment between the cap and the body is facilitated by thecap collar 30 which registers with theupper end 24 of the body 20 (see FIG. 4). - As is shown in FIGS. 4 and 5, the
tip holder 26 at itsupper end 34 has an enlarged diameter, and at its extreme upper end carries an outwardly extendingflange 36 which is engaged by thelip 38 on the lower end oflocknut 32. Thecylindrical chamber 40 within thetop end 34 ofholder 26 carries an O-ring 42 held in place by an O-ring retainer 44 also disposed in the enlargedtop end 34. The O-ring retainer 44 has an outwardly extendingflange 46 at its top, which abuts against theflange 36 of the tip holder. Thelocknut 32 retains the subassembly comprising the O-ring retainer 44 andtip holder 26 on adjustingscrew 48 that forms part of the metering assembly that is described in detail below. - A
piston 50 and guide rod 52 (hereinafter sometimes collectively called the pipetter piston 53) are coupled together at 54 and move reciprocally together within thebody 20 andtip holder 26. Theguide rod 52 at its upper end carries astem 56 which, in turn, carries a plunger button 58 (see FIGS. 4 and 12). Theplunger button 58 is positioned to be engaged by the thumb of the hand as suggested in FIG. 1 so as to move thepipetter piston 53 axially and downwardly in thebody 20 andtip holder 26 when fluid is to be expelled from thecylindrical passage 60 of thetip holder 26 and theaxial passage 61 in the disposable tip T. - The length of the vertical stroke of the
pipetter piston 53 is controlled by two separate stops mounted in thebody 20. The upper limit of the stroke of the pipetter piston is established by theadjustable stop 70 defined by theflange 72 of aspindle cap 74 which in turn is carried by aspindle 76. As is evident in FIGS. 4 and 5, thespindle 76 is threaded onto the adjustingscrew 48 and moves axially on it when the spindle is rotated. Thesecond stop 80 is established by awasher 82 which engages theshoulder 112 of thepiston assembly 53. - In the illustrated embodiment of this invention the
spindle cap 74 andspindle 76 are separately fabricated. Thespindle 76 at itsupper end 84 has inner andouter collars annular space 90 between them into which thecollar 92 on the lower end of thespindle cap 74 extends (see FIG. 5). The two parts may be ultrasonically welded together or otherwise secured together so that they are permanently fixed to one another and move as a unit on the adjustingscrew 48. Theflange 72 of thespindle cap 74 lies above theshoulder 94 of theguide rod 52 and is provided with asmall opening 96 at its center through which thestem 56 extends (see FIG. 4). Thus, the upward travel of thepipetter piston 53 is limited by engagement of theshoulder 94 on theguide rod 52 with theflange 72. The position of the flange may be varied by rotatingspindle 76 on the adjustingscrew 48. - As is described more fully below, the
spindle 76 may be viewed within thebody 20 of the pipetter through a window 100 (see FIG. 2), and the spindle carries aring 102 with a vernier scale which cooperates with ascale 104 in thewindow 100. These calibrations are a measure of the volume of fluid to be drawn into thepassage 61 in the tip T when thepipetter piston 53 is retracted, as is made clear below in connection with the description of the operation of the instrument. - The
lower stop 80 disposed within theaxial chamber 110 in adjustingscrew 48 cooperates with theshoulder 112 formed in theguide rod 52 and which faces downwardly in thepipetter body 20. Thewasher 82 that comprises the stop is yieldably held in place by a blow-outspring 114 which surrounds the reduceddiameter section 116 of the guide rod extending downwardly from theshoulder 112 to thecoupling 54 joining the lower end of theguide rod 52 and thepiston 50. The blow-outspring 114 bears against the upper face of aspring retainer 120 also mounted in thechamber 110 in adjustingscrew 48. Thus, thelower stop 80 is yieldable, as thewasher 82 may be moved downwardly against the bias of thespring 114 when engaged by theshoulder 112 as thespring 114 compresses. Thespring retainer 120 is described in further detail below in connection with the assembling and disassembling of the pipetter. - A
second coil spring 122 surrounds thepiston 50 and extends between thecoupling 54 and aseat 126 formed in the lower end of the O-ring retainer 44 (see FIG. 5). Thespring 122 serves as the main spring to yieldably oppose downward actuation of thepipetter piston 53. Any downward motion of thepipetter piston 53 causes thecoil spring 122 to compress (see FIG. 10), and whenever the actuatingbutton 58 is released, the pipetter piston returns to the upper position shown in FIG. 2. During the initial movement of thepipetter piston 53, only thespring 122 is compressed, and the resistance to actuation of the pipetter piston is essentially uniform throughout the major portion of the stroke established by thestops shoulders shoulder 112 engages the yieldable stop 80 (as in FIG. 10), further forward motion of thepipetter piston 53 is permitted only by compression of the blow-outspring 114 and continued compression of the spring 122 (see FIG. 11). Thus, substantially more resistance is encountered during the blow-out function of the pipetter, and a very clear "feel" is sensed by the user when the principal portion of the stroke is completed. - A
stripper assembly 150 is in part mounted within thebody 20 and in part lies below the body and surrounds thetip holder 26. Thestripper assembly 150 includes astem 152 mounted for reciprocal motion within thehousing 20 along a path parallel to the axis of the pipetter piston 53 (see FIG. 4). Therod 152 carries anactuating button 154 at itsupper end 156, which may be conveniently actuated by the thumb of the hand which grips thebody 20, as is evident in FIG. 1. Therod 152 is supported in anopening 158 inflange 160 formed as an integral part of thebody 20. Acompression spring 162 surrounds the upper portion of therod 152 between theflange 160 and thelower end 164 of actuatingbutton 154 and urges the rod upwardly in the body. The upward travel of therod 152 is limited by theshoulder 166 formed on the rod and which engages theflange 160. The downward travel of therod 150 is limited by engagement of theflange 170 of theactuating button 154 with theedge 172 of thebody 20. - The
lower end 176 of therod 152 has a threadedstem 178 of reduced diameter, which extends through theflange 179 of atubular stripper 181. The two are held in assembled relationship by the retainingnut 180. - The
stripper 181 is tapered slightly in a downward direction so that itslower end 182 has an inner diameter which is only slightly greater than the outer diameter of thetip holder 26. The inner diameter of thestripper 181 increases in an upward direction and is significantly enlarged at itsupper end 184 so as to surround thelocknut 32. The strength of thestripper 181 at its upper end is enhanced by therib 188 connected to theflange 179 at the location where the threadedstem 178 of therod 152 extends through the flange. - It is apparent that when the
actuating button 154 is depressed, therod 152 moves downwardly and carries thestripper 181 with it. The movement of the stripper is guided by thetip holder 26 which it surrounds at its lower end. As thestripper 181 moves downwardly about thetip holder 26, the removable tip T carried on the end of thetip holder 26 will be forced off it and fall away from the pipetter. - From the foregoing description it is evident that rotation of the
spindle 76 on the adjustingscrew 48 varies the position of theupper stop 70 which limits the extent that thepipetter piston 53 may be retracted into thebody 20. It is essential for accurate repeatability of the pipetter that any particular setting of thestop 70 be fixed when a series of liquid transfers is to be made by the device. In the present invention, the spindle may be locked against rotation on the adjusting screw by means of the lock slide 200 (see FIGS. 4, 6 and 7). Thelock slide 200 mounted inbody 20 is moved translationally in the body by means of afinger grip 202 mounted inslot 204 in thebody wall 206. The finger grip has an upwardly extendingarm 208 which extends along the outer surface of the adjustingscrew 48 and into the lower end of thecylindrical spindle 76. Thespindle 76 overlaps theupper end 210 of thearm 208 even in the uppermost position of the spindle as shown in FIG. 4. - As is evident in FIG. 7, a
ramp 212 is formed on the outer surface of the adjustingscrew 48, which cooperates with acam surface 214 on the inner face of theupper end 210 ofarm 208. When thelock slide 200 is moved upwardly by actuation of thefinger grip 202, thecam surface 214 rides upwardly on theramp 212 causing theend 210 of the arm to bear against and bind on the inner surface of thespindle 76. The friction contact between thearm 208 and the inner surface of the spindle prevents the spindle from rotating and, therefore, thestop 70 remains in a fixed position to insure accurate, repetitive metering of the fluid. The spindle will not be moved again until thelock slide 200 is released. - As is shown in FIGS. 4, 8, 9 and 12, the adjusting
screw 48 is split so as to divide its lower end into two finger-like portions 219. Each finger portion carries anear 220 on its outer surface that is sized to register with theopenings 222 in thebody wall 206. To mount the adjustingscrew 48 in place, thefinger portions 219 are squeezed together as in FIG. 9 so that they allow theears 220 to clear thelower edge 223 of the body and snap into theopenings 222. Once the adjustingscrew 48 is mounted in thebody 20 in that manner, thespring retainer 120 is inserted into the adjusting screw to prevent itsfinger portions 219 from collapsing toward one another and dislodging theears 220 from theopenings 222. It will be noted particularly in FIG. 12 that the lower end of theretainer 120 is tapered slightly, which causes the retainer to wedge in place within thescrew 48 and rigidly support the barrel shape of screw. To disassemble the unit, theretainer 120 may be pulled from the adjusting screw, and the adjusting screw may then be removed from thebody 20. Theretainer 120, as described above, also locks in the blow-outspring 114 that biases thepiston 53 upwardly in the assembly. Theretainer 120 may have projections 120' at its upper end that register with slots in the adjusting screw to form a bayonet connection between the two, to prevent the retainer from accidentally falling from the assembly (see FIGS. 4 and 12). - As described briefly above, a portion of the
spindle 76 is clearly visible through thewindow 100 in the body. Theportion 102 viewable through the window carries a vernier scale which cooperates with thelinear scale 104 on thewindow 100 so as to subdivide each unit of the linear scale into ten parts. The units, of course, are a function of the pitch of the adjusting screw and spindle, the diameter of thepipetter piston 53, etc. - In use, the pipetter of the present invention operates as follows:
Assume a reservoir of fluid to be transferred and a plurality of wells to receive the transferred liquid are provided, and a selected volume to be transferred from the reservoir to each of the wells is known. The operator rotates the exposed portion of the spindle so as to set it to the selected position on the scale visible through the window. Assume also that a rack of disposable pipetter tips T is provided which enables thetip holder 26 to be inserted in the appropriate end of the disposable tip and frictionally engage it. With the tip in place on theholder 26, and the setting having been made with the spindle to position thestop 70, the operator moves thelock slide 200 upwardly with thefinger grip 202 so that theupper end 210 of thearm 206 engages the inside of the spindle to hold it firmly against further rotation. The operator then depresses thebutton 58 carried by thespindle piston 53 until theshoulder 112 engages thestop 80. This position is shown in FIG. 13. Engagement of the stop by theshoulder 112 is readily sensed as significant additional resistance is encountered at that point because of the blow-outspring 114. With thepipetter piston 53 in that position, the tip T is placed in the reservoir with its lower end submerged in the liquid, and thebutton 58 is thereupon released allowing thepipetter piston 53 to rise under the influence of thespring 114 until theshoulder 94 of the piston engages theflange 72 as shown in FIG. 14. This action causes liquid to be drawn into the tip T in the volume set on the vernier scale. The pipetter is next positioned with the tip T aligned with the open end of the well W to which the liquid is to be transferred, and the operator again depresses thebutton 58 causing the pipetter piston to move downwardly. The pressure in thepassage 60 in the tip holder and thepassage 61 in the tip T causes the liquid in tip T to be discharged into the well. Essentially complete discharge occurs when theshoulder 112 again engages thestop 80 as in FIG. 15. However, to ensure total discharge of the liquid in the pipetter, the operator depresses thebutton 58 beyond that point, which causes the piston to move further downwardly, andring 82 slides downwardly and compresses the blow-outspring 114 as in FIG. 16. This action ensures the complete discharge of fluid from the pipetter. With the pipetter tip T removed from the well the operator then need merely release thebutton 58, and themain spring 122 of the metering assembly along with the blow-outspring 114 will return the pipetter piston to the fully retracted position shown in FIG. 2. If the same amount of fluid is to be transferred to yet another well, the foregoing procedure may be repeated. If the tip T is to be removed, the operator need only press thebutton 154 and move therod 152 against the bias ofspring 162 until theflange 170 of the button engages theend 172 of thebody 20. This action will cause thestripper 181 to move downwardly on the tip holder and cause thelower end 182 of the stripper to engage the tip T and force it off theholder 26 as suggested in FIG. 17. - From the foregoing description many of the advantages of the present invention will be apparent. For example, the pipetter of the present invention may very quickly and easily be adjusted to vary the volume of liquid drawn into the device upon actuation of the
button 58. The adjustment is made simply by turning the spindle which is exposed through thewindow 100. Simply by spinning thespindle 76, thetop stop 70 may be positioned so as to cause the pipetter piston to draw in the desired volume of liquid when the pipetter piston is depressed and then released. The vernier scale visible in the window enables the user to quickly set the stop to provide the desired metering. While the spindle is very easily turned, it may quickly and firmly be locked in any set position by means of thelock slide 200. By moving the slide upwardly toward the top end of the body in the slot provided in the outer wall, thearm 208 will wedge underneath the lower end of thespindle 76 and prevent it from rotating and changing the position ofstop 70. This will ensure accurate repeatability of the device as it is used over and over again to transfer like quantities of liquid. Though the lock is very effective and easy to operate, it may be readily disengaged merely by sliding the lock slide downwardly by means of thefinger grip 202 so that thearm 208 releases the spindle. - It will be appreciated that a certain amount of drag is imposed on the spindle by virtue of the contact of
stop 70 carried by the spindle with theshoulder 94 at the upper end of the pipetter piston. That drag is sufficient to prevent the spindle from spinning freely on the adjusting screw. If a major change is to be made in the setting of the stop requiring that the spindle be rotated through many revolutions on the screw, the operator may eliminate the drag by slightly depressing thebutton 58 to separate the pipetter piston from the stop. The spindle may then very easily and quickly be spun through a substantial number of turns to make a gross adjustment. - Another advantage of the present invention is the elimination in the device of screw machined parts. All but a few of the parts of the device may be injection molded of a suitable plastic material. This mode of construction is reflected in reduced manufacturing costs for the device. Manufacturing costs are also minimized by the presence of only a few ultrasonically welded parts. In the construction of the pipetter described, only two ultrasonic welds are included, namely, at the attachment of the
button 58 to thepipetter shaft 53 and at the connection of thestop 70 to thespindle 76. - Another advantage of the present invention is the ease with which the device may be taken apart for cleaning or sterilization. Merely by removing retaining
nut 180 which connects the end of therod 152 to thestripper 181, the stripper may be removed from the assembly so as to expose the full length of thetip holder 26 and the coupling which joins the tip holder to the adjustingscrew 48 andbody 20. More particularly, removal of the stripper exposes thelocknut 32 which may then be turned so as to free theflange 36 of the tip holder. And with the tip holder removed, theretainer nut 44 may be removed, which will allow thepiston 50 to be withdrawn. This is particularly convenient for the user who may wish to autoclave thepiston 50 if, for example, fluid has accidentally entered thepassage 60 in the tip holder from thepassage 61 in the tip T. By removing the blow-outspring retainer 120 from the adjustingscrew 48 and theplunger button 58 from thestem 56 of theguide rod 52, the blow-outspring 114,washer 82 and guiderod 52 may also be withdrawn from thebody 20 through its lower end. The device may be further disassembled by squeezing thefingers 219 of thesplit adjusting screw 48 together to release theears 220 from theopenings 222. - It is not intended that the scope of this invention be limited to the single embodiment illustrated and described. Rather, it is intended that the scope of this invention be determined by the appended claims and their equivalents.
Claims (14)
- A pipetter comprising:
an elongated tubular body having an axis and a cylinder defining a discharge volume;
a tubular adjusting screw mounted coaxially within, and rigidly attached to, the body, the adjusting screw having an external screw thread on a portion thereof;
a manually-rotatable, tubular spindle fitting over the adjusting screw and having an internal screw thread which mates with the adjusting screw external screw thread so that the spindle can be adjusted axially with respect to the adjusting screw;
a piston extending through the adjusting screw and the spindle into the cylinder, the piston being axially movable towards the spindle during an intake stroke and axially movable towards the adjusting screw during a discharge stroke; and
a stop connected to the spindle for limiting the axial movement of the piston during the intake stroke. - a pipetter as defined in Claim 1 further comprising an axial scale on an unthreaded portion of the adjusting screw and a circular scale on an end of the spindle which fits over the adjusting screw, the circular scale cooperating with the axial scale so that the spindle is repeatably adjustable relative to the adjusting screw in fractional screw thread increments.
- A pipetter as defined in Claim 2 wherein the body has a window therein so that the axial scale and the circular scale are viewable.
- A pipetter as defined in Claim 3 wherein the spindle is engageable through the window for manual rotation.
- A pipetter as defined in Claim 1 wherein the piston has a radial shoulder contained within the spindle and the stop comprises a cap connected on one spindle end, the cap having a hole therethrough and the piston extending through the hole so that the shoulder contacts the cap and limits movement of the piston during the intake stroke.
- A pipetter as defined in Claim 1 further comprising a compression coil spring fitting over the piston and located between the piston and the adjusting screw, the spring urging the piston towards the spindle.
- A pipetter as defined in claim 6 wherein a second stop is mounted in the body in the path of the pipetter piston for resisting movement of the piston in a downward direction beyond a preselected position and a second spring is mounted in the body to engage the second stop for yieldably holding the second stop in the preselected position but allowing the piston to move beyond that position.
- A pipetter as defined in Claim 1 wherein the adjusting screw is removably attached to the body by means of a plurality of flexible ears, which ears fit into slots in the body.
- A pipetter as defined in claim 1 wherein the body has an upper and a lower end and wherein the pipetter further comprises:
a tip holder attached to the lower end of the body and having an opening therethrough axially aligned with the body axis, the tip holder having a lower end for engaging and carrying removable tips;
an elongated stripper surrounding a portion of the tip holder and movable axially thereon for removing a tip mounted on the lower end of the tip holder; and
an actuating rod mounted in the body and attached to the stripper for imparting motion to the stripper parallel to the body axis so as to operate the stripper. - A pipetter as defined in claim 9 wherein the actuating rod is removably coupled to the stripper outside the body.
- A pipetter as defined in claim 9 wherein a coupling removably connects the tip holder to the adjusting screw, and the stripper encloses the coupling.
- A pipetter as defined in claim 1 wherein manually operable means are mounted in the body for releasably engaging the spindle for preventing the spindle from rotating.
- A pipetter as defined in claim 12 wherein the spindle engaging means is a slide which frictionally engages the spindle so as to prevent rotation thereof.
- A pipetter as defined in claim 1 wherein the adjusting screw is constructed to be compressible radially for insertion into the body and means are removably mounted in the adjusting screw to prevent the screw from being compressed radially after the adjusting screw is inserted in the body.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US42440889A | 1989-10-20 | 1989-10-20 | |
US424408 | 1989-10-20 | ||
PCT/US1990/005800 WO1991005609A1 (en) | 1989-10-20 | 1990-10-10 | Pipetter |
Publications (3)
Publication Number | Publication Date |
---|---|
EP0496784A1 EP0496784A1 (en) | 1992-08-05 |
EP0496784A4 EP0496784A4 (en) | 1992-09-09 |
EP0496784B1 true EP0496784B1 (en) | 1994-04-27 |
Family
ID=23682526
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP90915595A Expired - Lifetime EP0496784B1 (en) | 1989-10-20 | 1990-10-10 | Pipetter |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0496784B1 (en) |
JP (1) | JPH05501222A (en) |
DE (1) | DE69008540T2 (en) |
WO (1) | WO1991005609A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2826722A1 (en) | 2013-07-19 | 2015-01-21 | Mettler-Toledo AG | Handheld dosing device for powders and pastes |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5435355A (en) * | 1992-10-09 | 1995-07-25 | Xiao; Yong | Conical floating type fuel filling device |
DE9415847U1 (en) * | 1994-09-30 | 1994-12-08 | Ritter Ralf | Repeater pipette |
US5983733A (en) * | 1996-11-15 | 1999-11-16 | Hamilton Company | Manual pipette |
JP3473409B2 (en) * | 1998-06-11 | 2003-12-02 | 松下電器産業株式会社 | Dispensing device |
US6428750B1 (en) * | 2000-02-17 | 2002-08-06 | Rainin Instrument, Llc | Volume adjustable manual pipette with quick set volume adjustment |
FI20002241A0 (en) | 2000-10-11 | 2000-10-11 | Labsystems Oy | Adjustable pipette |
FIU20010274U0 (en) * | 2001-07-06 | 2001-07-06 | Biohit Oyj | The suction device |
JP4148894B2 (en) | 2001-10-16 | 2008-09-10 | マトリックス・テクノロジイズ・コーポレーション | Hand-held pipette |
DE102004003434B4 (en) * | 2004-01-21 | 2006-06-08 | Eppendorf Ag | Pipetting device with a displacement device and a drive device detachably connected thereto |
FI20050340A0 (en) * | 2005-04-01 | 2005-04-01 | Thermo Electron Oy | Calibratable pipette |
DE102005041183B3 (en) * | 2005-08-31 | 2007-01-04 | Eppendorf Ag | Two-part pipetting system for metering liquids, has lower spigot which is secured in its holder by snap hooks with catches and released by wedge system actuated by movement of unlocking ring |
JP5657861B2 (en) * | 2008-12-30 | 2015-01-21 | 光陽化成有限会社 | Micropipette device |
WO2016002019A1 (en) * | 2014-07-01 | 2016-01-07 | 株式会社安川電機 | Liquid transport system and liquid transport method |
EP3112026B1 (en) | 2015-06-30 | 2018-03-14 | Sartorius Biohit Liquid Handling Oy | Pipette with a tip removal mechanism, a method for removing a tip, and a method for pipetting |
JP7315625B2 (en) * | 2020-07-14 | 2023-07-26 | エッペンドルフ エスイー | Pipettes for use with pipette tips with integral plungers |
FR3128889B1 (en) * | 2021-11-09 | 2023-09-29 | Gilson Sas | POSITIVE DISPLACEMENT SAMPLING DEVICE, DESIGNED TO IMPROVE THE GRIP OF THE PISTON OF A PISTON-CAPILLARY SYSTEM |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU6276773A (en) * | 1972-11-21 | 1975-05-22 | Excalibur Lab Pty Ltd | Pipette |
US4054062A (en) * | 1976-05-03 | 1977-10-18 | Oxford Laboratories Inc. | Hand-held micropipettor with improved accuracy of liquid volumes transferred |
FI57540C (en) * | 1977-02-17 | 1980-09-10 | Suovaniemi Finnpipette | ANORDNING FOER ATT LOESGOERA OCH AVLAEGSNA EN ENGAONGSSPETS PAO EN PIPETT |
FI57543C (en) * | 1978-06-02 | 1980-09-10 | Suovaniemi Finnpipette | VOLUMREGLERBAR PIPETT |
FI56937C (en) * | 1978-08-04 | 1980-05-12 | Suovaniemi Finnpipette | PIPETT ANORDNING FOER ATT AVLAEGSNA EN ENGAONGSSPETSBEHAOLLARE FRAON EN PIPETT |
JP2554666B2 (en) * | 1987-09-18 | 1996-11-13 | 株式会社ニチリョー | Variable pipette |
-
1990
- 1990-10-10 JP JP51457790A patent/JPH05501222A/en active Pending
- 1990-10-10 WO PCT/US1990/005800 patent/WO1991005609A1/en active IP Right Grant
- 1990-10-10 DE DE69008540T patent/DE69008540T2/en not_active Expired - Fee Related
- 1990-10-10 EP EP90915595A patent/EP0496784B1/en not_active Expired - Lifetime
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2826722A1 (en) | 2013-07-19 | 2015-01-21 | Mettler-Toledo AG | Handheld dosing device for powders and pastes |
US9517849B2 (en) | 2013-07-19 | 2016-12-13 | Mettler-Toledo Gmbh | Handheld dosage-dispensing instrument for powderous or pasteous dosage material |
Also Published As
Publication number | Publication date |
---|---|
DE69008540T2 (en) | 1994-12-15 |
DE69008540D1 (en) | 1994-06-01 |
WO1991005609A1 (en) | 1991-05-02 |
JPH05501222A (en) | 1993-03-11 |
EP0496784A4 (en) | 1992-09-09 |
EP0496784A1 (en) | 1992-08-05 |
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