EP0404783A1 - Immunoanalyses a l'aide d'anticorps monoclonaux dresses contre des proteines de liaison naturelles - Google Patents

Immunoanalyses a l'aide d'anticorps monoclonaux dresses contre des proteines de liaison naturelles

Info

Publication number
EP0404783A1
EP0404783A1 EP89902678A EP89902678A EP0404783A1 EP 0404783 A1 EP0404783 A1 EP 0404783A1 EP 89902678 A EP89902678 A EP 89902678A EP 89902678 A EP89902678 A EP 89902678A EP 0404783 A1 EP0404783 A1 EP 0404783A1
Authority
EP
European Patent Office
Prior art keywords
binding protein
binding
ligand
antibody
labeled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP89902678A
Other languages
German (de)
English (en)
Other versions
EP0404783A4 (en
Inventor
Charles Walter Benner
Michael Terry Largen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of EP0404783A1 publication Critical patent/EP0404783A1/fr
Publication of EP0404783A4 publication Critical patent/EP0404783A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens

Definitions

  • Monoclonal antibodies to naturally occurring binding proteins which fall within the scope of this invention include but are not limited to the following functionalities: 1) antibodies which recognize binding protein and do not interfere with binding of ligand by the binding protein; and
  • the preferred class of antibodies which bind to binding protein irrespective of whether ligand is bound and which do not interfere with subsequent binding of ligand antibodies, are expected to produce a positive result in the RISA as well as in the direct and indirect ELISA assays.
  • Antibodies presumed to bind binding protein only when ligand is not bound would be expected to be positive in the direct ELISA and RISA and would be expected to be negative in the indirect ELISA. Based upon these primary screening results antibodies can be further characterized as to their abilities to react with binding protein under the various conditions of analyte binding.
  • FBP anti-FBP-AP complex was prepared by combining equal volumes of FBP diluted 1:1000 with 50 mM sodium tetraborate, 150 mM NaCl, 1.0 mM MgCl2, 0.1 mM ZnCl2, 0.1% rabbit IgG (Sigma, used as a stabilizer), pH 9.3, and anti-FBP-AP conjugate diluted 1:200 with the same buffer.

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

On utilise des anticorps monoclonaux ou des fragments d'anticorps monoclonaux immunoréactifs spécifiques contre des protéines de liaison se produisant naturellement, afin de former un complexe anticorps monoclonaux-protéines de liaison par lequel on peut détecter un ligand dans une variété de formats d'immunoanalyses. Ces anticorps ou fragments peuvent être marqués, et lorsqu'ils ne sont pas marqués, on fait réagir l'anticorps ou le fragment non marqués avec un anticorps marqué spécifique contre l'anticorps ou le fragment non marqués. Cette approche préserve l'affinité inhérente de la protéine de liaison pour le ligand.
EP19890902678 1988-01-28 1989-01-27 Immunoassays using monoclonal antibodies directed against natural binding proteins Withdrawn EP0404783A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14950988A 1988-01-28 1988-01-28
US149509 1998-09-08

Publications (2)

Publication Number Publication Date
EP0404783A1 true EP0404783A1 (fr) 1991-01-02
EP0404783A4 EP0404783A4 (en) 1991-01-23

Family

ID=22530614

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19890902678 Withdrawn EP0404783A4 (en) 1988-01-28 1989-01-27 Immunoassays using monoclonal antibodies directed against natural binding proteins

Country Status (4)

Country Link
EP (1) EP0404783A4 (fr)
JP (1) JPH03503566A (fr)
GB (1) GB2233091B (fr)
WO (1) WO1989007271A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210017A (en) * 1990-11-19 1993-05-11 Genentech, Inc. Ligand-mediated immunofunctional hormone binding protein assay method
US6326154B1 (en) 1990-11-19 2001-12-04 Genentech, Inc. Ligand-mediated immunofunctional hormone binding protein assay method
US5593844A (en) * 1990-11-19 1997-01-14 Genentech, Inc. Ligand-mediated immunofunctional hormone binding protein assay method
US5434087A (en) * 1993-02-24 1995-07-18 Abbott Laboratories Folate immunoassay utilizing folate binding protein in a multiclonal antibody format
CN109991411B (zh) * 2017-12-29 2023-01-20 上海索昕生物科技有限公司 一种检测待测样本中目标抗体的免疫测定方法及其应用
CN112129951A (zh) * 2020-09-16 2020-12-25 武汉生之源生物科技股份有限公司 一种saa化学发光酶免疫检测酶标稀释液及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0019277A1 (fr) * 1979-05-21 1980-11-26 New York Blood Center, Inc. Procédé de détection d'un antigène dans un échantillon

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0133540A1 (fr) * 1983-08-03 1985-02-27 E.I. Du Pont De Nemours And Company Déterminations d'un récepteur employant des anticorps marqués monoclonaux et anti-idiotypiques
US4594327A (en) * 1983-11-02 1986-06-10 Syntex (U.S.A.) Inc. Assay method for whole blood samples
US4786593A (en) * 1985-04-16 1988-11-22 Wistar Institute Of Anatomy And Biology Diagnostic method for detection of neural crest disease
US4742000A (en) * 1986-05-02 1988-05-03 University Of Chicago Antibody to human progesterone receptor and diagnostic materials and methods

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0019277A1 (fr) * 1979-05-21 1980-11-26 New York Blood Center, Inc. Procédé de détection d'un antigène dans un échantillon

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO8907271A1 *

Also Published As

Publication number Publication date
GB2233091A (en) 1991-01-02
EP0404783A4 (en) 1991-01-23
JPH03503566A (ja) 1991-08-08
GB9015666D0 (en) 1990-09-26
GB2233091B (en) 1992-05-27
WO1989007271A1 (fr) 1989-08-10

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