GB2233091A - Immunoassays using monoclonal antibodies directed against natural binding proteins - Google Patents

Immunoassays using monoclonal antibodies directed against natural binding proteins

Info

Publication number
GB2233091A
GB2233091A GB9015666A GB9015666A GB2233091A GB 2233091 A GB2233091 A GB 2233091A GB 9015666 A GB9015666 A GB 9015666A GB 9015666 A GB9015666 A GB 9015666A GB 2233091 A GB2233091 A GB 2233091A
Authority
GB
United Kingdom
Prior art keywords
binding proteins
monoclonal antibodies
unlabeled
immunoassays
directed against
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9015666A
Other versions
GB2233091B (en
GB9015666D0 (en
Inventor
Charles Walter Benner
Michael Terry Largen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of GB9015666D0 publication Critical patent/GB9015666D0/en
Publication of GB2233091A publication Critical patent/GB2233091A/en
Application granted granted Critical
Publication of GB2233091B publication Critical patent/GB2233091B/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Monoclonal antibodies or immunoreactive monoclonal antibody fragments specific for naturally occurring binding proteins are used to form a monoclonal antibody-binding protein complex by which to detect a ligand in a variety of immuoassay formats. These antibodies or fragments can be labeled or unlabeled and when they are unlabeled then the unlabeled antibody or fragment is reacted with a labeled antibody specific for the unlabeled antibody or fragment. This approach preserves the inherent affinity of the binding protein for the ligand.
GB9015666A 1988-01-28 1989-01-27 Immunoassays using monoclonal antibodies directed against natural binding proteins Expired - Lifetime GB2233091B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14950988A 1988-01-28 1988-01-28
PCT/US1989/000342 WO1989007271A1 (en) 1988-01-28 1989-01-27 Immunoassays using monoclonal antibodies directed against natural binding proteins

Publications (3)

Publication Number Publication Date
GB9015666D0 GB9015666D0 (en) 1990-09-26
GB2233091A true GB2233091A (en) 1991-01-02
GB2233091B GB2233091B (en) 1992-05-27

Family

ID=22530614

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9015666A Expired - Lifetime GB2233091B (en) 1988-01-28 1989-01-27 Immunoassays using monoclonal antibodies directed against natural binding proteins

Country Status (4)

Country Link
EP (1) EP0404783A4 (en)
JP (1) JPH03503566A (en)
GB (1) GB2233091B (en)
WO (1) WO1989007271A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6326154B1 (en) 1990-11-19 2001-12-04 Genentech, Inc. Ligand-mediated immunofunctional hormone binding protein assay method
US5210017A (en) * 1990-11-19 1993-05-11 Genentech, Inc. Ligand-mediated immunofunctional hormone binding protein assay method
US5593844A (en) * 1990-11-19 1997-01-14 Genentech, Inc. Ligand-mediated immunofunctional hormone binding protein assay method
US5434087A (en) * 1993-02-24 1995-07-18 Abbott Laboratories Folate immunoassay utilizing folate binding protein in a multiclonal antibody format
CN109991411B (en) * 2017-12-29 2023-01-20 上海索昕生物科技有限公司 Immunoassay method for detecting target antibody in sample to be detected and application thereof
CN112129951A (en) * 2020-09-16 2020-12-25 武汉生之源生物科技股份有限公司 Enzyme-labeled diluent for SAA chemiluminescence enzyme immunoassay and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0133540A1 (en) * 1983-08-03 1985-02-27 E.I. Du Pont De Nemours And Company Receptor assays using labeled monoclonal anti-idiotypic antibodies
US4594327A (en) * 1983-11-02 1986-06-10 Syntex (U.S.A.) Inc. Assay method for whole blood samples
US4742000A (en) * 1986-05-02 1988-05-03 University Of Chicago Antibody to human progesterone receptor and diagnostic materials and methods
US4786593A (en) * 1985-04-16 1988-11-22 Wistar Institute Of Anatomy And Biology Diagnostic method for detection of neural crest disease

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4495296A (en) * 1979-05-21 1985-01-22 New York Blood Center, Inc. Labeled anti-hapten antibodies and their use as a universal reagent for solid phase radio- and/or enzyme-immunoassays

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0133540A1 (en) * 1983-08-03 1985-02-27 E.I. Du Pont De Nemours And Company Receptor assays using labeled monoclonal anti-idiotypic antibodies
US4594327A (en) * 1983-11-02 1986-06-10 Syntex (U.S.A.) Inc. Assay method for whole blood samples
US4786593A (en) * 1985-04-16 1988-11-22 Wistar Institute Of Anatomy And Biology Diagnostic method for detection of neural crest disease
US4742000A (en) * 1986-05-02 1988-05-03 University Of Chicago Antibody to human progesterone receptor and diagnostic materials and methods

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Analytical Biochemistry 1985, 149, 555-565 *
Proc. Natl. Acad. Sci. USA 1980, 77(1), 157-161 *
Reviews of Infectious Diseases 1982, 4(1), 35-41 *

Also Published As

Publication number Publication date
GB2233091B (en) 1992-05-27
EP0404783A4 (en) 1991-01-23
EP0404783A1 (en) 1991-01-02
GB9015666D0 (en) 1990-09-26
JPH03503566A (en) 1991-08-08
WO1989007271A1 (en) 1989-08-10

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19950127