EP0397827A1 - Procédé pour la lutte biologique contre la varroatose et dispositif pour la mise en oeuvre de ce procédé - Google Patents

Procédé pour la lutte biologique contre la varroatose et dispositif pour la mise en oeuvre de ce procédé

Info

Publication number
EP0397827A1
EP0397827A1 EP89912630A EP89912630A EP0397827A1 EP 0397827 A1 EP0397827 A1 EP 0397827A1 EP 89912630 A EP89912630 A EP 89912630A EP 89912630 A EP89912630 A EP 89912630A EP 0397827 A1 EP0397827 A1 EP 0397827A1
Authority
EP
European Patent Office
Prior art keywords
mite
attractive product
methyl
varroa jacobsoni
attractive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP89912630A
Other languages
German (de)
English (en)
French (fr)
Inventor
Gérard ARNOLD
Claude Masson
Yves Le Conte
Jérôme TROUILLER
Bertrand Chappe
Guy Ourisson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Institut National de la Recherche Agronomique INRA
Original Assignee
Centre National de la Recherche Scientifique CNRS
Institut National de la Recherche Agronomique INRA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Institut National de la Recherche Agronomique INRA filed Critical Centre National de la Recherche Scientifique CNRS
Publication of EP0397827A1 publication Critical patent/EP0397827A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K51/00—Appliances for treating beehives or parts thereof, e.g. for cleaning or disinfecting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10—Animals; Substances produced thereby or obtained therefrom
    • A01N63/14—Insects

Definitions

  • the present invention relates to a biological control method against varroatosis, a serious parasitosis of the bee which is due to Varroa jacobsoni Oud., A specific ectoparasitic mite of the genus Apis.
  • This biological control process has two aspects: first that of the diagnosis of this parasitosis, and, if it is present, the trapping and destruction of the mite.
  • the present invention also relates to trapping devices for implementing this method.
  • Varroa jacobsoni finds, in the colonies of the honey bee Apis MeIlifica, the optimal climatic and trophic conditions which it needs to develop. By removing the hemolyrnph from workers and males - in adults as well as in larvae and nymphs, it causes significant disturbances in these insects, which generally lead to their early death. The bee colony is therefore very weak, and it disappears after about 4 years. In addition, the parasitized colonies are rapidly contaminated by secondary infections, in particular viral infections, which further accelerate their extinction. For example, a survey carried out by the Union of Honey Producers of France at the end of 1986 revealed that, in the Mediterranean region, all the colonies were parasitized, and that a large part of them they were in a critical situation.
  • Varroa jacobsoni The only treatments currently effective against Varroa jacobsoni are chemical treatments based on acaricide molecules.
  • the use of these products has various drawbacks: on the one hand, the mites become more or less quickly resistant to these molecules, and, on the other hand, they risk tarnishing the image of healthy products and beehives and especially honey. Although no trace of residue has ever been detected in France, it is not possible to predict the future on this subject.
  • the mode of use of these molecules can have certain drawbacks: if their duration of action is short (from a few hours to a few days), the numerous varroa mites, which are found inside the sealed cells, are not affected by the molecules and will emerge a few days later to reproduce in turn and prolong the infestation; such methods therefore have limited effectiveness. Conversely, if their duration of action is long (a few weeks), the molecules can pass into wax or honey after a certain time and contaminate them. In addition, the use of acaricides for long periods is not without presenting risks of toxicity to the bees and themselves.
  • the active principles of these kairomones are at the base of the biological control process which is the subject of the present invention and the development of which has become urgent given the fact that, as indicated above, this mite currently represents, at the level international, the most serious danger which threatens the colonies of the bee the Apis mell ifica.
  • the present invention therefore relates firstly to a method for the biological control of
  • Varroa jacobsoni characterized in that an attractive product consisting of:
  • a total hexanic extract of bee larvae in particular male larvae and worker larvae, in particular male larvae (drones), these larvae being especially bee larvae Apis mellifica ligustica; an active fraction (that is to say a fraction responsible for the attraction of Varroa jacobsoni) of a total hexane extract of bee larvae, this fraction resulting in particular from a fractionation on a silica column of l 'above-mentioned total hexane extract; at least one active ester derived from a C 1 -C 6 aliphatic alcohol, such as methanol and ethanol, and from a C 10 -C 24 carboxylic acid, with a straight branched chain, saturated or comprising one or more several cis or trans double bonds, conjugated or not, a benzene ring or a heterocycle which may be included in the chain of said acid;
  • an active fraction that is to say a fraction responsible for the attraction of Varroa
  • the attractive product is introduced into the medium where the Varroa jacobsoni mite can be present, at a temperature between 30 and 36 ° C.
  • this adjuvant being chosen in particular from organic esters which are more volatile than those indicated above and which do not have the attractive activity according to the invention.
  • the attractive product is applied in the hive, at the entrance to the hive or outside the hive, within a trap, preventing, if necessary, by means of a protective grid, the bees from entering said trap.
  • this process is advantageously combined with a heat treatment, so as to increase its efficiency.
  • methyl palmitate in particular is very active at 34 ° C, but that it is no longer active at 22 ° C; however, it turns out that 34 ° C is the average temperature of the bee brood.
  • 34 ° C is the average temperature of the bee brood.
  • the adequate temperature for its effectiveness is slightly higher than its melting point (28-30 ° C).
  • the biological control method according to the invention has two complementary aspects:
  • the beekeeper will thus be informed of the presence of the parasite and of the importance of the infestation and therefore possibly carry out a controlled application of acaricide treatment;
  • the mites being eliminated by means of traps (the active molecules being used in chemical decoys), by simply being trapped, for example by an adhesive substance, or killed , for example, by a contact miticide.
  • the present invention also relates to a device for implementing the method as it has just been defined, this device being characterized in that it consists in a trap capable of attracting the Varroa jacobsoni mite by the presence of the attractive product as defined above, optionally combined with at least one adjuvant intended to increase the volatility thereof, and / or to modulate the rate of volatilization thereof, and / or at least one inert diluent, and / or at least one hormone.
  • the trap comprises a support carrying an adhesive coating in which is incorporated a quantity of attractive product, and, where appropriate, of a pesticide, an acaricide and / or a sterilizing agent, or a porous support impregnated with an amount of attractant and, if necessary, of pesticide, acaricide, and / or sterilizing agent.
  • a support carrying an adhesive coating in which is incorporated a quantity of attractive product, and, where appropriate, of a pesticide, an acaricide and / or a sterilizing agent, or a porous support impregnated with an amount of attractant and, if necessary, of pesticide, acaricide, and / or sterilizing agent.
  • an elongated support which will be placed flat on the floor of the hive, in the center thereof.
  • the trap consists of an open housing containing the attractive product, if necessary associated with at least one adjuvant as defined above, and / or with at least one pesticide, an acaricide and / or a sterilizing agent, the opening of said housing being able to be closed by a grid preventing the bees from entering said housing.
  • the devices according to the invention can also be associated with a heating means, including a heating resistor, so as to increase the efficiency of the process.
  • the main advantage of the process according to the invention is to use molecules which are generally present naturally even in the colony of bees, and which therefore do not risk polluting the various products of the hive: honey, pollen , royal jelly, propolis, wax. It is a process that is easy to use, and is suitable for both professional beekeepers and amateurs.
  • the male bee larvae Apis mellifica ligustica were removed two days before the cells were sealed. Three hundred larvae were extracted in 50 ml of hexane, for one hour, at room temperature (6 equivalent larvae (Eql) per ml).
  • carrier gas hydrogen at 4 x 10 4 Pa
  • carrier gas hydrogen 4 x 10 4 Pa.
  • the CG / SM coupling unit is composed of a Varian 3400 chromatograph equipped with a Split Splitless injector and a CW 20M column (length: 50 m, diameter: 0.32 mm), helium carrier gas and a Finigan Mat Incos 50 mass spectrometer.
  • the resulting chroma togr amm ⁇ is represented in FIG. 1 e) Identification if ication:
  • the device was placed in an enclosure thermostatically controlled at 32 ⁇ 1 ° C (relative humidity: 90 ⁇ 5%), which is the temperature corresponding to the thermal preference of the mite (Le Conte and Arnold, ApidoIogie, 16 (2), pages 155-164 (1988). of Varroa jacobsoni in the different fields was analyzed according to two complementary methods:
  • Figure 2 of the accompanying drawing shows the spatial distribution of Varroa jacobsoni females in the olfactometer.
  • methyl palmitate two of the four fields (upper left and lower right) are odorized with palmitate. methyl; the other two fields are odorless.
  • Figure 3 illustrates the olfactory responses of Varroa jacobsoni females to methyl palmitate and other compounds from extracts of male bee larvae.
  • Each field of a dynamic four-choice olfactometer was traversed by an air flow, at the speed of 0.9 ⁇ 0.1 l / h. Depending on the case, one or two of the fields were odorized, the others being odorless.
  • the bee larvae, or the various extracts and compounds whose biological efficacy was to be tested, were placed in a glass vial connected to an arm of the olfactometer.
  • the extracts were deposited on a 2 x 2 Whatman (GF / F) filter paper. cm; the other vials receiving a control filter paper of the same dimensions.
  • the chemical extracts were renewed every two experiments; previously, the olfactometer was cleaned with alcohol and rinsed with distilled water. The device was rotated 90 ° after 7 consecutive tests, to eliminate interference from other signals in the orientation of the mites.
  • Varroas used were female lees taken from a colony of bees and kept at 22 ° C for 7 to 9 days on workers in a breeding crate.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Insects & Arthropods (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP89912630A 1988-11-03 1989-10-31 Procédé pour la lutte biologique contre la varroatose et dispositif pour la mise en oeuvre de ce procédé Withdrawn EP0397827A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8814349A FR2638326B1 (fr) 1988-11-03 1988-11-03 Procede pour la lutte biologique contre la varroatose et dispositifs pour la mise en oeuvre de ce procede
FR8814349 1988-11-03

Publications (1)

Publication Number Publication Date
EP0397827A1 true EP0397827A1 (fr) 1990-11-22

Family

ID=9371535

Family Applications (1)

Application Number Title Priority Date Filing Date
EP89912630A Withdrawn EP0397827A1 (fr) 1988-11-03 1989-10-31 Procédé pour la lutte biologique contre la varroatose et dispositif pour la mise en oeuvre de ce procédé

Country Status (10)

Country Link
US (1) US5135758A (US06272168-20010807-M00014.png)
EP (1) EP0397827A1 (US06272168-20010807-M00014.png)
JP (1) JPH03502104A (US06272168-20010807-M00014.png)
BG (1) BG51146A3 (US06272168-20010807-M00014.png)
DK (1) DK158190D0 (US06272168-20010807-M00014.png)
ES (1) ES2018415A6 (US06272168-20010807-M00014.png)
FR (1) FR2638326B1 (US06272168-20010807-M00014.png)
GR (1) GR1000417B (US06272168-20010807-M00014.png)
HU (1) HUT54291A (US06272168-20010807-M00014.png)
WO (1) WO1990004922A1 (US06272168-20010807-M00014.png)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2665610B1 (fr) * 1990-08-10 1994-03-11 Centre Nal Recherc Scientifique Pheromone anti-oviposition comprenant au moins un alkyl ester d'acide gras, et son application a la lutte contre les insectes ravageurs.
FR2672467B1 (fr) * 1991-02-11 1993-05-21 Robert Jean Edouard Composition acaricide utilisable contre la varroatose des abeilles et dispositif la renfermant.
WO1993000812A1 (fr) * 1991-07-02 1993-01-21 Institut National De La Recherche Agronomique (I.N.R.A.) Compositions anti-oviposition comprenant un acide gras et/ou un alkyl ester d'acide gras et eventuellement un monoterpene
FR2678483A1 (fr) * 1991-07-02 1993-01-08 Agronomique Inst Nat Rech Pheromone anti-oviposition comprenant au moins un acide gras et/ou un alkyl ester d'acide gras, et son application a la lutte contre les lepidopteres tortricides.
FR2720897A1 (fr) * 1994-06-14 1995-12-15 Atochem Elf Sa Compositions insecticides à base d'undécylénate de méthyle.
US6595828B2 (en) 2001-02-02 2003-07-22 The Regents Of The University Of California Synthetic bee pollen foraging pheromone and uses thereof
ITUD20020247A1 (it) * 2002-11-26 2004-05-27 Univ Degli Studi Udine Procedimento per controllare e condizionare il comportamento dell'acaro varroa destructor in un alveare, o in un altro ambiente in cui si riproducono le api.
CA2838705A1 (en) 2011-06-06 2012-12-13 John I. Haas, Inc. Compositions and methods for controlling a honey bee parasitic mite infestation
EP2695517B1 (en) * 2012-08-09 2019-02-06 Interbran Nature GmbH Pheromone composition for treating Varroa mite infestation
BR112015016129A2 (pt) 2013-01-07 2017-07-11 Haas Inc John I tira ondulada para uso na redução de uma infestação por ácaro parasita da abelha melífera; tira ondulada preparada pela imersão de uma tira absorvente em uma composição líquida; embalagem de alumínio preparada pela imersão de uma tira absorvente ondulada em uma composição líquida; colmeia feita pelo homem; produto da abelha melífera; método de redução de uma infestação por ácaro parasita da abelha melífera em uma colmeia de abelhas melíferas; e kit para o tratamento ou prevenção de uma infestação por ácaro parasita
RU2600707C2 (ru) * 2013-02-26 2016-10-27 Государственное научное учреждение Всероссийский научно-исследовательский институт экспериментальной ветеринарии имени Я.Р. Коваленко (ВИЭВ) Россельхозакадемии Способ оздоровления семей пчел от варрооза
DE202013007841U1 (de) * 2013-09-04 2013-11-26 Bayer Animal Health Gmbh Vorrichtung zur Untersuchung des Befalls von Honigbienenvölkern mit Varroamilben
CA2944363A1 (en) 2014-03-24 2015-10-01 Simon Fraser University Honey bee mite disruptive compounds and methods of application
FR3066884B1 (fr) * 2017-06-06 2020-12-18 Agronomique Inst Nat Rech Procede de selection de colonies d’abeilles presentant le caractere vsh et composition et kit pour sa mise en œuvre
CA3043388A1 (en) 2018-05-14 2019-11-14 John I. Hass, Inc. Compositions and methods for controlling a honey bee parasitic mite infestation
DE102020111422A1 (de) 2020-04-27 2021-10-28 Technische Universität München, Körperschaft des öffentlichen Rechts Parasitenschutzvorrichtung für Bienenstöcke und Parasitenschutzverfahren

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1105069A (fr) * 1953-07-20 1955-11-28 Boehringer Sohn Ingelheim Perfectionnements apportés aux produits et procédés pour combattre les charançons et les bostrychidés
DE2719722C3 (de) * 1977-05-03 1982-04-22 Vsesojuznyj nauČno-issledovatel'skij institut veterinarnoj sanitarii, Moskva Bekämpfung von Ektoparasiten der Bienen
DE3538688A1 (de) * 1985-10-31 1987-05-07 Bayer Ag Verfahren zur bekaempfung von parasitosen bei bienen
BE905621A (fr) * 1986-10-17 1987-04-17 Sanders Probel Biotechnology S Methode et dispositif de piegeage de parasites des abeilles, et substances destinees a cette methode et a ce dispositif.

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9004922A1 *

Also Published As

Publication number Publication date
GR1000417B (el) 1992-06-30
HU896917D0 (US06272168-20010807-M00014.png) 1990-12-28
BG51146A3 (en) 1993-02-15
HUT54291A (en) 1991-02-28
GR890100725A (en) 1990-12-31
DK158190A (da) 1990-06-29
JPH03502104A (ja) 1991-05-16
WO1990004922A1 (fr) 1990-05-17
FR2638326A1 (fr) 1990-05-04
FR2638326B1 (fr) 1991-01-25
ES2018415A6 (es) 1991-04-01
DK158190D0 (da) 1990-06-29
US5135758A (en) 1992-08-04

Similar Documents

Publication Publication Date Title
WO1990004922A1 (fr) Procede pour la lutte biologique contre la varroatose et dispositif pour la mise en ×uvre de ce procede
Landolt et al. Spotted wing drosophila, Drosophila suzukii (Diptera: Drosophilidae), trapped with combinations of wines and vinegars
Szendrei et al. Identification and field evaluation of attractants for the cranberry weevil, Anthonomus musculus Say
El-Sayed et al. Apple volatiles synergize the response of codling moth to pear ester
Kubik et al. Residues of captan (contact) and difenoconazole (systemic) fungicides in bee products from an apple orchard
US9326507B2 (en) Pheromone composition for treating Varroa mite infestation
Favaro et al. Impact of chronic exposure to two neonicotinoids on honey bee antennal responses to flower volatiles and pheromonal compounds
Akantetou et al. Evaluation du potentiel insecticide de l’huile essentielle de Ocimum canum Sims sur Aphis gossypii Glover (Homoptera: Aphididae) au Togo
Dekebo et al. Olfactory responses of Aethina tumida Murray (Coleoptera: Nitidulidae) to some major volatile compounds from hive materials and workers of Apis mellifera
FR3035775A1 (fr) Composition attractive pour la bruche de la feverole
AU2005232292B2 (en) Pheromone attractants for the green mirid
Kraft et al. Could plant extracts have enabled hominins to acquire honey before the control of fire?
FR3017510A1 (fr) Composition phytosanitaire
CA2002071A1 (fr) Procede pour la lutte biologique contre la varroatose et dispositifs pour la mise en oeuvre de ce procede
Shelly Do females of Bactrocera dorsalis (Hendel)(Diptera: Tephritidae) gain an indirect benefit by mating with methyl eugenol–fed males? Assessing the possibility that sons of methyl eugenol–fed fathers show enhanced lure responsiveness and mating success
EP3874948B1 (fr) Composition attractive pour les mâles et les femelles bruches de la féverole
EP0495955A1 (fr) Pheromone anti-oviposition comprenant au moins un alkyl ester d'acide gras, et son application a la lutte contre les insectes ravageurs
Hock et al. Behavioral responses of plum curculio (Coleoptera: Curculionidae) to different enantiomer concentrations and blends of the synthetic aggregation pheromone grandisoic acid
Shelly et al. Exposure to ginger root oil decreases capture of male Mediterranean fruit flies (Diptera: Tephritidae) in trimedlure-baited traps
Katsoyannos et al. Effect of citrus peel substances on male Mediterranean fruit fly behaviour.
EP3791722A1 (fr) Composition repulsive et utilisation
US20060165743A1 (en) Method and device to control and condition the behavior of the varroa destructor mite in a beehive, or in other environment where bees reproduce
CS201021B2 (en) Bait for insects
Attia et al. Persistence of toxicity in four natural extracts controlling Tetranychus urticae as affected by Tween® 20 supply
Koczur et al. Defensive secretion of Eurycantha calcarata-chemical composition and method of collection

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19900626

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH DE FR GB IT LI LU NL SE

17Q First examination report despatched

Effective date: 19920923

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 19940503