EP0351717A3 - Plasmide zur Erzeugung von alpha-Amylase in Bacillus - Google Patents

Plasmide zur Erzeugung von alpha-Amylase in Bacillus Download PDF

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Publication number
EP0351717A3
EP0351717A3 EP89112826A EP89112826A EP0351717A3 EP 0351717 A3 EP0351717 A3 EP 0351717A3 EP 89112826 A EP89112826 A EP 89112826A EP 89112826 A EP89112826 A EP 89112826A EP 0351717 A3 EP0351717 A3 EP 0351717A3
Authority
EP
European Patent Office
Prior art keywords
bacillus
amylase
plasmid
alpha
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP89112826A
Other languages
English (en)
French (fr)
Other versions
EP0351717A2 (de
Inventor
Jungo Okada
Urs HÄNGGI
Harald Dr. Berger
Konrad Dr. Gamon
Martina Markgraf
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Henkel AG and Co KGaA
Original Assignee
Henkel AG and Co KGaA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henkel AG and Co KGaA filed Critical Henkel AG and Co KGaA
Publication of EP0351717A2 publication Critical patent/EP0351717A2/de
Publication of EP0351717A3 publication Critical patent/EP0351717A3/de
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

In Bacillus exprimierbares Bacillus Plasmid, enthaltend einen Promotor für die Expression von Proteinen und in funktions­ fähiger Weise damit verknüpft das Strukturgen eines Enzyms, dadurch gekennzeichnet, daß es einen Protease-Promotor ver­ knüpft mit einem Amylase-Strukturgen enthält.
EP89112826A 1988-07-21 1989-07-13 Plasmide zur Erzeugung von alpha-Amylase in Bacillus Withdrawn EP0351717A3 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3824827 1988-07-21
DE3824827A DE3824827A1 (de) 1988-07-21 1988-07-21 Plasmide zur erzeugung von alpha-amylase in bacillus

Publications (2)

Publication Number Publication Date
EP0351717A2 EP0351717A2 (de) 1990-01-24
EP0351717A3 true EP0351717A3 (de) 1990-07-25

Family

ID=6359255

Family Applications (1)

Application Number Title Priority Date Filing Date
EP89112826A Withdrawn EP0351717A3 (de) 1988-07-21 1989-07-13 Plasmide zur Erzeugung von alpha-Amylase in Bacillus

Country Status (6)

Country Link
EP (1) EP0351717A3 (de)
JP (1) JPH0276587A (de)
KR (1) KR900001851A (de)
DE (1) DE3824827A1 (de)
DK (1) DK360789A (de)
FI (1) FI893503A (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3928046A1 (de) * 1989-08-25 1991-02-28 Henkel Kgaa Hybridplasmide zur erzeugung von subtilisin carlsberg in bacillus
GB2279955B (en) 1993-07-15 1998-02-18 Solvay Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof
GB0511602D0 (en) 2005-06-07 2005-07-13 Tmo Biotec Ltd Microorganisms
CA2660486A1 (en) 2006-09-28 2008-04-03 Tmo Renewables Limited Thermophilic microorganisms for ethanol production
GB0715751D0 (en) * 2007-08-13 2007-09-19 Tmo Renewables Ltd Thermophilic micro-organisms for ethanol production
GB0820262D0 (en) 2008-11-05 2008-12-10 Tmo Renewables Ltd Microorganisms

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2171703A (en) * 1985-01-18 1986-09-03 Agency Ind Science Techn Expression-secretion vector
EP0205371A1 (de) * 1985-05-22 1986-12-17 Centre National De La Recherche Scientifique (Cnrs) Expressionsvektoren für alpha-Amylase in Bacillus subtilis, erhaltene Stämme und Verfahren zur Herstellung von alpha-Amylase
EP0214435A2 (de) * 1985-08-03 1987-03-18 Henkel Kommanditgesellschaft auf Aktien Alkalische Protease, Verfahren zur Herstellung von Hybridvektoren und genetisch transformierte Mikroorganismen
EP0227260A1 (de) * 1985-10-25 1987-07-01 Omnigene Inc Erhöhung der Expression von Genen in Mikroorganismen

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2171703A (en) * 1985-01-18 1986-09-03 Agency Ind Science Techn Expression-secretion vector
EP0205371A1 (de) * 1985-05-22 1986-12-17 Centre National De La Recherche Scientifique (Cnrs) Expressionsvektoren für alpha-Amylase in Bacillus subtilis, erhaltene Stämme und Verfahren zur Herstellung von alpha-Amylase
EP0214435A2 (de) * 1985-08-03 1987-03-18 Henkel Kommanditgesellschaft auf Aktien Alkalische Protease, Verfahren zur Herstellung von Hybridvektoren und genetisch transformierte Mikroorganismen
EP0227260A1 (de) * 1985-10-25 1987-07-01 Omnigene Inc Erhöhung der Expression von Genen in Mikroorganismen

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ABSTRACTS OF PAPERS AMERICAN CHEMICAL SOCIETY, Band 194, Nr. 0, 1987, Seite MBTD118, Washington D.C., USA; ALAN P. SCOMA et al.: "Development of a unique and efficient secretion vector in bacillus-subtilis". *
GENE, Band 23, 1983, Seiten 267-276, Amsterdam, NL; STEPHEN A. ORTLEPP et al.: "Molecular cloning in Bacillus subtilis of a Bacillus licheniformis gene encoding a thermostable alpha amylase". *

Also Published As

Publication number Publication date
FI893503A0 (fi) 1989-07-20
KR900001851A (ko) 1990-02-27
DK360789A (da) 1990-01-22
FI893503A (fi) 1990-01-22
DK360789D0 (da) 1989-07-20
EP0351717A2 (de) 1990-01-24
DE3824827A1 (de) 1990-02-01
JPH0276587A (ja) 1990-03-15

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