EP0296254B1 - Zentrifugenrotor - Google Patents

Zentrifugenrotor Download PDF

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Publication number
EP0296254B1
EP0296254B1 EP87108855A EP87108855A EP0296254B1 EP 0296254 B1 EP0296254 B1 EP 0296254B1 EP 87108855 A EP87108855 A EP 87108855A EP 87108855 A EP87108855 A EP 87108855A EP 0296254 B1 EP0296254 B1 EP 0296254B1
Authority
EP
European Patent Office
Prior art keywords
rotor
test tubes
centrifuge
spring
guide inserts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP87108855A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP0296254A1 (de
Inventor
Peter Dipl.-Ing. Bader
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf Netheler Hinz GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf Netheler Hinz GmbH filed Critical Eppendorf Netheler Hinz GmbH
Priority to DE8787108855T priority Critical patent/DE3768808D1/de
Priority to AT87108855T priority patent/ATE61742T1/de
Priority to EP87108855A priority patent/EP0296254B1/de
Priority to JP63150398A priority patent/JPS6418458A/ja
Priority to US07/209,495 priority patent/US4832679A/en
Publication of EP0296254A1 publication Critical patent/EP0296254A1/de
Application granted granted Critical
Publication of EP0296254B1 publication Critical patent/EP0296254B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes

Definitions

  • the invention relates to a centrifuge rotor with a lower part and an upper part, on which openings are provided for the use of sample vessels with an edge system, a collecting channel formed by upward deformation of the edge being provided on the outside of the lower part, and sample vessels being introduced into these openings and further There is a gap running between the upper part and lower part.
  • Such a centrifuge rotor is known from US-A-4,484,906.
  • the sample vessels are inserted into openings in a central wall part of the upper part that tapers downwards and are supported on the conical wall part with outer edge flanges arranged at the openings. Its inclination is dimensioned such that when the edge flanges are in contact, the sample vessels lie against an outer wall part of the upper part that widens downwards.
  • the gutter should take up any liquid escaping from damaged vessels.
  • the gap running around is able to absorb tolerances between the upper part and lower part, but it is shielded by an angled edge-like extension of the upper part, so that a free passage of air is impaired.
  • the lower part has receptacles for the sample vessels which run downwards and outwards in the conical direction.
  • the receptacles are open at the top and laterally to the outside, so that the sample vessels can be inserted laterally from the outside and guided laterally when the rotor is open.
  • These lateral openings formed in this way can be covered by the outer part in a surface in which the outermost wall part of the vessels is located.
  • the outer part also forms a cover for the upper end of the vessels and the lower part as the upper part.
  • This version is advantageous, but if the version consists of only two parts, the vessels must be inserted and held transversely to their longitudinal axis. The upper part then only forms a hedge. The upper and lower part must be designed to match each other. This requires time-consuming and costly milling work.
  • the load on the sealingly interlocking edges of the upper and lower parts is in the order of magnitude of up to 15,000 rpm. considerably.
  • the distribution of the support of sample vessels on the lower part and the upper part leads to uncertainties.
  • centrifuge rotor which also has open side receptacles for the use of sample vessels.
  • these receptacles are open at an angle downwards in a three-part centrifuge rotor within an insert and are then closed off by a lower part into which the insert can be inserted, which is held on the outside in an upper part with a correspondingly inclined wall part.
  • This design is not only complex, but also very difficult to use, because practically the use as an intermediate part or insert with the recesses for the sample vessels must first be held in the opposite direction until the sample vessels are inserted. After that, the holder can be fixed from above by using the lower part and only then is it reversed for use in the lower part.
  • the vessel receiving part has a conical shape internally tapering edge section with receiving openings, on the edge of which outward abutment edges of the sample vessels are placed.
  • an obliquely outward-facing wall of the receiving part is then arranged and directed with respect to the receiving openings in such a way that the inserted sample vessels, which can initially hang down, lie against this wall at operating speeds with a central section.
  • the overall arrangement is then covered and held together by an attached top part.
  • a collecting trough is provided on the lower part, which is formed by shaping the edge of the lower part upwards and inwards. It is covered by the edge of the receiving part. Therefore, it is doubtful whether this gutter does its job, because a possible breathing between a lower edge web of the vessel receiving part and the gutter of the lower part can lead to the fact that in the case of defective vessels, escaping substances are sprayed out.
  • a one-piece centrifuge rotor with a cover which can be fitted is known, the lower part of which has both the openings for receiving the vessel edges and is bent inwards with its lower edge in order to form a collecting trough.
  • the vessels are only held at the openings with their upper flanged edges, so that no secure position is created. A wall installation on the outer wall of the lower part is not possible. Vascular vibrations result.
  • the invention has for its object to provide a two-part centrifuge rotor of the type mentioned above, which is easy to manufacture, easy to use and also easy to clean, but which significantly improves the holding of sample vessels in the idle state and also at maximum circulation speeds becomes. The same also applies to changing speeds, so that increased safety is ensured even when the centrifuge rotor is only in two parts.
  • Such a spring arrangement ensures an always constant alignment of the sample vessels, so that a secure mounting and a secure alignment both at a standstill and at the highest rotational speed is available.
  • the spring arrangement prevents the sample containers from rattling when the rotor is at a standstill, and the sample containers unintentionally slide out even when the sample is easily removed.
  • the segmentation exerts equally large forces on each individual vessel.
  • the effect of the spring arrangement is improved by the formation of spring lamellae and the conical angling of the outer edge.
  • the segmentation or lamination results in a position stabilized in the circumferential direction. Due to the conical bending, a flat system is created. As a result, the spring transmits its force to the vessels over a relatively large area, so that deformations and material abrasion or wear of the spring are avoided.
  • the wall of the upper part which widens conically downwards, can expand to a certain extent under the centrifugal action, which is absorbed by the gap, then even at operating speed there is a flat contact, in particular of the guide insert on the outer wall.
  • sleeve-shaped cylindrical guide inserts are introduced into the opening, which have an outwardly flanged edge as the upper abutment and are open at the bottom and which end at a distance from the lower part, into which guide inserts sample containers can be inserted.
  • the spring arrangement lies against the inside of the guide inserts.
  • Such guide inserts are used in particular at higher speeds (12000 min -1 ) in order to relieve the load on the sample vessels, which are preferably made of plastic.
  • a spring arrangement according to the design described allows the easy replacement of any soiled or damaged sleeves after the customer has dismantled the rotor.
  • the open design of the guide inserts avoids swinging air columns, so that a cover can be omitted upwards and the spring arrangement ensures a quiet guidance regardless of the filling of inserted sample vessels.
  • An embodiment is created in which a one-part segmented spring arrangement either presses loose guide inserts or inserted sample vessels outwards against the wall of the conical upper part. It is particularly advantageous if the outer edge of a spring lamella has a concave groove which receives a cylindrical guide insert. As a result, the guide inserts are held in a certain position, excluding any tolerance.
  • the spring arrangement is advantageously a flat perforated disk between the fastening flange of the hub body on the one hand and a flange-like wall section of one of the elements which comprise the upper part and the lower part , on the other hand is integrated, the integration being carried out by means of the fastening means which is provided for fixing the flange-like sections of the upper part and the lower part.
  • the integration is advantageously carried out on an edge flange of the lower part, because then there is an increased distance from the openings for the cylindrical guide inserts, but in particular also the sample vessels.
  • the centrifuge rotor has a lower part 1 and an upper part 2, in which openings 3, 4, 5 ... are provided for the use of sample vessels or guide inserts 6, 7 for them.
  • Such guide inserts or sample vessels have outward flange-like upper flanged edges 8, 9, which are located on the opening edges of the upper part invest.
  • the latter then has a wall 10 which widens conically downwards and forms an edge contact for the guide inserts or sample vessels inserted into the openings 3 to 5.
  • a hub body 11 which has a cylindrical part 12 and a lower, outwardly directed fastening flange 13.
  • a flange-like wall section 14 of the upper part 2 and a corresponding flange-like wall section 15 of the lower part 1 are arranged at the top in step-shaped recesses 16, 17 designed according to their wall thickness and are fixed by means of fastening screw arrangements 18 guided through openings.
  • fastening screw arrangements 18 guided through openings.
  • three fastening screw arrangements offset around the circumference are provided.
  • a disk-shaped spring arrangement 21 is integrated between the fastening flange 13 and the edge flange 15 of the lower part 1, which is also shown in plan view in FIG. 2.
  • This spring arrangement is a spring washer which has slots 25, 26, 27, 28 directed radially from the outside in order to form spring plates 22, 23, 24 .... distributed uniformly around the circumference.
  • a circumferential gap 35 is arranged on the outside between upper part 2 and lower part 1.
  • the gap 35 is formed downwards by the upper retracted edge 36 of the lower part 1, which is designed to form a collecting channel 37 below the cylindrical guide inserts or sample vessels.
  • the upper edge of the gap 35 is formed by the lower edge 38 of the upper part 2, namely the lower edge of the conical wall 10, on which the vessels or guide inserts abut. This edge can be bevelled to the extent that it runs essentially axially parallel to the hub body 11.
  • the lower edge of the upper part is so far below the upper edge of the lower part that by centrifuge galkraft directly accelerated parts cannot escape.
  • the hub body 11 has a downward central opening 39, into which the shaft journal of a rotor drive can be inserted.
  • the shaft journal has a threaded opening at the top, into which a threaded pin 40 of a fastening screw 41 can be screwed, which is guided and held on the outside of the hub body 11 with a head 42 so that it cannot be lost, but can be rotated and moved in height.

Landscapes

  • Centrifugal Separators (AREA)
EP87108855A 1987-06-20 1987-06-20 Zentrifugenrotor Expired - Lifetime EP0296254B1 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE8787108855T DE3768808D1 (de) 1987-06-20 1987-06-20 Zentrifugenrotor.
AT87108855T ATE61742T1 (de) 1987-06-20 1987-06-20 Zentrifugenrotor.
EP87108855A EP0296254B1 (de) 1987-06-20 1987-06-20 Zentrifugenrotor
JP63150398A JPS6418458A (en) 1987-06-20 1988-06-20 Centrifugal separating rotor
US07/209,495 US4832679A (en) 1987-06-20 1988-06-20 Rotor for centrifuge

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP87108855A EP0296254B1 (de) 1987-06-20 1987-06-20 Zentrifugenrotor

Publications (2)

Publication Number Publication Date
EP0296254A1 EP0296254A1 (de) 1988-12-28
EP0296254B1 true EP0296254B1 (de) 1991-03-20

Family

ID=8197075

Family Applications (1)

Application Number Title Priority Date Filing Date
EP87108855A Expired - Lifetime EP0296254B1 (de) 1987-06-20 1987-06-20 Zentrifugenrotor

Country Status (5)

Country Link
US (1) US4832679A (enExample)
EP (1) EP0296254B1 (enExample)
JP (1) JPS6418458A (enExample)
AT (1) ATE61742T1 (enExample)
DE (1) DE3768808D1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4014440C1 (enExample) * 1990-05-05 1991-07-04 Heraeus Sepatech Gmbh, 3360 Osterode, De
DE9016288U1 (de) * 1990-11-30 1991-10-10 Fa. Andreas Hettich, 7200 Tuttlingen Winkelkopf für Zentrifugen
DE4233753C2 (de) * 1992-10-07 1998-01-29 Hettich Andreas Fa Vakuum-Zentrifuge
KR960700102A (ko) * 1993-01-14 1996-01-19 게셈 맬리크마다니 모하메드 초경량 복합재질로 된 원심분리기 로터(ultra-light composite centrifuge rotor)
US5362300A (en) * 1993-05-27 1994-11-08 E. I. Du Pont De Nemours And Company Shell-type centrifuge rotor
US5487719A (en) * 1994-01-14 1996-01-30 Denver Instrument Company Centrifuge rotor assembly
US5562583A (en) * 1995-09-07 1996-10-08 E. I. Du Pont De Nemours And Company Tube adapter for centrifuge shell type rotor
US5728038A (en) * 1997-04-25 1998-03-17 Beckman Instruments, Inc. Centrifuge rotor having structural stress relief
USD441873S1 (en) 1999-07-21 2001-05-08 Eppendorf Ag Rotor for a centrifuge
US6190300B1 (en) 2000-03-10 2001-02-20 Labnet International Inc. Centrifuge rotor adapted for use with centrifuge tube strips
DE102004062231B4 (de) * 2004-12-23 2012-12-13 Thermo Electron Led Gmbh Rotor für Laborzentrifugen
DE102004062232B4 (de) * 2004-12-23 2013-01-10 Thermo Electron Led Gmbh Rotor für Laborzentrifugen
JP4941877B2 (ja) * 2005-10-18 2012-05-30 日立工機株式会社 遠心分離機用ロータ及び遠心分離機
JP4862711B2 (ja) * 2007-03-20 2012-01-25 日立工機株式会社 遠心分離機用ロータ及び遠心分離機
US20100038294A1 (en) * 2008-08-12 2010-02-18 Charles Dugan Centrifugal separation apparatus
CN114591806B (zh) * 2022-03-08 2026-03-24 魏小兰 一种游离dna的提取系统

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3709429A (en) * 1970-10-19 1973-01-09 Kenzie D Mc Centrifuges
US3825178A (en) * 1971-12-17 1974-07-23 American Hospital Supply Corp Centrifuge rotor
JPS57937Y2 (enExample) * 1978-03-13 1982-01-07
JPS541069U (enExample) * 1978-05-16 1979-01-06
JPS5740112U (enExample) * 1980-08-16 1982-03-04
DE97809T1 (de) * 1982-06-09 1984-04-26 Shandon Southern Products Ltd., Runcorn, Cheshire Zentrifugierung.
US4412830A (en) * 1982-06-24 1983-11-01 Beckman Instruments, Inc. Cover for centrifuge rotor
US4449965A (en) * 1982-10-04 1984-05-22 Beckman Instruments, Inc. Shell type centrifuge rotor having controlled windage
US4484906A (en) * 1983-05-02 1984-11-27 Beckman Instruments, Inc. Shell type centrifuge rotor retaining ruptured tube sample
DE3341323C2 (de) * 1983-11-15 1987-02-19 Heraeus Separationstechnik GmbH, 3360 Osterode Laboratoriumszentrifuge
US4553955A (en) * 1984-06-01 1985-11-19 Beckman Instruments, Inc. Multi-angle adapter for fixed angle centrifuge rotor

Also Published As

Publication number Publication date
JPH0238264B2 (enExample) 1990-08-29
JPS6418458A (en) 1989-01-23
US4832679A (en) 1989-05-23
ATE61742T1 (de) 1991-04-15
DE3768808D1 (de) 1991-04-25
EP0296254A1 (de) 1988-12-28

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