EP0271153A2 - Enzyme enthaltendes Reinigungsmittel - Google Patents

Enzyme enthaltendes Reinigungsmittel Download PDF

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Publication number
EP0271153A2
EP0271153A2 EP87202385A EP87202385A EP0271153A2 EP 0271153 A2 EP0271153 A2 EP 0271153A2 EP 87202385 A EP87202385 A EP 87202385A EP 87202385 A EP87202385 A EP 87202385A EP 0271153 A2 EP0271153 A2 EP 0271153A2
Authority
EP
European Patent Office
Prior art keywords
lipase
lipases
detergent
pseudomonas
detergent composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP87202385A
Other languages
English (en)
French (fr)
Other versions
EP0271153B1 (de
EP0271153A3 (en
Inventor
Hendrik Egbert De Jong
Ton Swarthoff
David Thom
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever PLC
Unilever NV
Original Assignee
Unilever PLC
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever PLC, Unilever NV filed Critical Unilever PLC
Publication of EP0271153A2 publication Critical patent/EP0271153A2/de
Publication of EP0271153A3 publication Critical patent/EP0271153A3/en
Application granted granted Critical
Publication of EP0271153B1 publication Critical patent/EP0271153B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase

Definitions

  • the present invention relates to an enzymatic detergent composition
  • an enzymatic detergent composition comprising as detergent active material solely a nonionic synthetic detergent, and a mixture of lipolytic and proteolytic enzymes.
  • Lipolytic enzymes are well known in detergent compositions. Lipolytic enzymes have been mentioned in the prior art as possible enzymes for detergent compositions, but there is relatively little prior art directly concerned with lipases for inclusion in detergent compositions.
  • nonionic synthetic detergents in general do not negatively influence the activity of proteolytic and lipolytic enzymes, in contrast with e.g. anionic synthetic detergents, which often negatively influence the proteases and lipases.
  • lipases are basically proteins, they would be susceptible to proteolytic action by the proteases if lipases were used in conjunction with proteases. It would therefore be expected that the inclusion of proteases in a detergent composition comprising lipases and as detergent-active material solely a nonionic detergent surfactant (which does not affect either the lipase or the protease), would cause the lipase activity to be destroyed by the proteolytic action of the proteases.
  • the class of lipases to be used according to the present invention embraces those lipases which show a positive immunological cross-reaction with the antibody of the lipase produced by the microorganism Chromobacter viscosum var. lipolyticum NRRL B 3673.
  • This lipase has been described in Dutch Patent Specification 154,269 of Toyo Jozo KK, and the microorganism is available to the public from the collection of the United States Department of Agriculture, Agricultural Research Service, Northern Utilization and Development Division, Peoria, Illinois, under N o NRRL B 3673.
  • This lipase will be referred to hereinafter as "Toyo Jozo" lipase.
  • the lipases of the present invention should show a positive immunological cross-reaction with the Toyo Jozo lipase antibody, using the standard and well-known immunodiffusion procedure according to Ouchterlony (Acta. Med. Scan., 133 , pages 76-79 (1950)).
  • the preparation of the antiserum is carried out as follows: Equal volumes of 0.1 mg/ml antigen and of Freund's adjuvant (complete or incomplete) are mixed until an emulsion is obtained. Two female rabbits are injected with 2 ml samples of the emulsion according to the following scheme: day 0 : antigen in complete Freund's adjuvant day 4 : antigen in complete Freund's adjuvant day 32 : antigen in incomplete Freund's adjuvant day 60 : booster of antigen in incomplete Freund's adjuvant.
  • the serum containing the required antibody is prepared by centrifugation of clotted blood, taken on day 67.
  • the titre of the anti-Toyo Jozo-lipase antiserum is determined by the inspection of precipitation of serial dilutions of antigen and antiserum according to the Ouchterlony procedure. A 25 dilution of antiserum was the dilution that still gave a visible precipitation with an antigen concentration of 0.1 mg/ml.
  • lipases showing a positive immunological cross-­reaction with the Toyo Jozo lipase antibody as hereabove described are lipases according to the present invention. Typical examples thereof are the lipase ex Pseudomonas fluorescens IAM 1057 (available under the trade name Amano-P), the lipase ex Pseudomonas fragi FERM P 1339 (available under the trade name Amano-B), lipase ex Pseudomonas nitroreducens var. lipolyticum FERM P 1338, the lipase ex Pseudomonas sp.
  • the lipase ex Pseudomonas fluorescens IAM 1057 available under the trade name Amano-P
  • the lipase ex Pseudomonas fragi FERM P 1339 available under the trade name Amano-B
  • lipases ex Chromobacter viscosum , e.g. Chromobacter viscosum var. lipolyticum NRRL B 3673, commercially available from Toyo Jozo Co., Tagata, Japan; and further C . viscosum lipases from US Biochemical Corp., U.S.A., and Diosynth Co., The Netherlands, and lipases ex Pseudomonas gladioli .
  • the lipases of the present invention are included in the detergent composition in such an amount that the final detergent composition has a lipolytic enzyme activity of from 100 to 0.005 LU/mg, preferably 25 to 0.05 LU/mg of the composition.
  • lipases can be used in their non-purified form or in a purified form, e.g. purified with the aid of well-­known adsorption methods, such as phenyl sepharose adsorption techniques.
  • proteases used according to the present invention are the well-known detergent proteases and mixtures thereof.
  • the proteases are used in such an amount that the final detergent composition has a proteolytic activity of 0.1 to 50 GU/mg, preferably 0.5 to 30 GU/mg.
  • GU Glycine Unit; one GU is the amount of enzyme which under standard incubation conditions produces an amount of terminal NH2-groups equivalent to 1 microgr./ml glycine.
  • the detergent composition further comprises as detergent-active material solely a nonionic synthetic detergent.
  • This type of detergent is well known in the art.
  • Nonionics usually consist of a hydrophobic moiety derived from fatty alcohols, fatty acid amides and alkylphenols, and a hydrophilic moiety consisting of alkylene oxide units. Typical examples are the condensation products of alkylene oxides such as ethylene oxide, propylene oxide and butylene oxide and mixtures thereof with C8-C18 primary or secondary, branched or straight chain alcohols, C8-C18 fatty acid amides, C9-C18 alkylphenols, etc. Further suitable examples are given in M. Schick "Nonionic surfactants" (1967).
  • the composition contains from 1-50%, usually from 2-45%, and commonly from 5-30% by weight of the nonionic detergent. Mixtures of various nonionic detergents may also be used.
  • the detergent composition may furthermore include the usual detergent ingredients in the usual amounts.
  • the composition may contain from 1-60%, preferably from 5-30% by weight of one or more organic and/or inorganic builders. Furthermore, it may contain from 1-35% of a bleaching agent or a bleaching system comprising a bleaching agent and an activator therefor, e.g. sodium perborate with tetraacetyl ethylene diamine.
  • compositions may furthermore comprise lather boosters, foam depressors, anti-corrosion agents, soil-­suspending agents, sequestering agents, anti-soil redeposition agents, perfumes, dyes, solvents, stabilising agents for the enzymes and bleaching agents and so on. They may also comprise from 0.01-10% by weight of enzymes other than lipases and proteases, such as amylases, oxidases and cellulases.
  • compositions of the present invention can be formulated in any desired form, such as powders, bars, pastes, liquids, etc.
  • washing experiments were carried out in a Tergotometer under the following conditions: wash time and temperature: 10 minutes at 40°C; three rinses with cold water water hardness: 14 and 40°FH detergent composition concentration: 2 and 6 g/l number soil/wash cycles : 4 protease concentration: 20 GU/ml lipase concentration: 1 LU/ml As lipases were used: Amano-P, Diosynth and a lipase ex Pseudomonas gladioli The detergent composition used had the following formulation:
  • the reflectance of the test cloths and the residual weight percentage of fatty material on the test cloths were determined after the fourth wash cycle.
  • the reflectance was measured in a Reflectometer at 460 nm with a UV filter in the light pathway, and the fatty matter by extracting the dried test cloths with petroleum ether, and determining the amount of fatty matter from the weight loss of the test cloth.
  • Example 1 The procedure of Example 1 was repeated, but under the following conditions: detergent composition concentration: 2 g/l number of soil/wash cycles : 4 wash time and temperature: 10 minutes at 40°C, three rinses with cold water water hardness: 14°FH agitation: 100 rpm lipase: ex Pseudomonas gladioli 1 LU/ml protease: 20 GU/ml As proteases, Savinase, Alcalase, Maxatase, Maxacal, Kazuzase and Esperase were used.
  • the stability of lipases according to the present invention in the presence of proteases and varying amounts of a nonionic detergent was tested by determining the residual lipase activity with the pH-­stat. method.
  • a composition comprising 0.7 g pentasodium triphosphate, 0.7 g sodium sulphate and 0.2 sodium silicate and varying amounts of C13-C15 alcohol, condensed with 7 and 3 moles of ethylene oxide, respectively (product A and product B) was used.
  • the temperature was 30°C, the water hardness 14°FH and the pH 9.5.
  • the lipase was either lipase ex Pseudomonas gladioli , or Amano-P, at 15 LU/ml, and the protease was Savinase, at 20 GU/ml.
  • Example 1 was repeated, using various lipases. The following results were obtained:

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Detergent Compositions (AREA)
EP87202385A 1986-12-10 1987-12-02 Enzyme enthaltendes Reinigungsmittel Expired - Lifetime EP0271153B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB868629535A GB8629535D0 (en) 1986-12-10 1986-12-10 Enzymatic detergent composition
GB8629535 1986-12-10

Publications (3)

Publication Number Publication Date
EP0271153A2 true EP0271153A2 (de) 1988-06-15
EP0271153A3 EP0271153A3 (en) 1988-08-10
EP0271153B1 EP0271153B1 (de) 1990-03-14

Family

ID=10608785

Family Applications (1)

Application Number Title Priority Date Filing Date
EP87202385A Expired - Lifetime EP0271153B1 (de) 1986-12-10 1987-12-02 Enzyme enthaltendes Reinigungsmittel

Country Status (9)

Country Link
EP (1) EP0271153B1 (de)
JP (1) JPH0696717B2 (de)
AU (1) AU605806B2 (de)
BR (1) BR8706682A (de)
CA (1) CA1288368C (de)
DE (1) DE3761910D1 (de)
ES (1) ES2014465B3 (de)
GB (1) GB8629535D0 (de)
ZA (1) ZA879296B (de)

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989004361A1 (en) * 1987-11-02 1989-05-18 Novo-Nordisk A/S Enzymatic detergent composition
EP0368589A2 (de) * 1988-11-08 1990-05-16 Unilever Plc Enzymhaltige Waschmittel
US4933287A (en) * 1985-08-09 1990-06-12 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
US4950417A (en) * 1989-05-01 1990-08-21 Miles Inc. Detergent formulations containing alkaline lipase derived from Pseudomonas plantarii
WO1990009440A1 (en) * 1989-02-20 1990-08-23 Novo Nordisk A/S Enzyme containing granulate and method for production thereof
EP0407225A1 (de) * 1989-07-07 1991-01-09 Unilever Plc Enzyme und enzymhaltiges Reinigungsmittel
US5100796A (en) * 1988-02-22 1992-03-31 Synfina-Oleofina Methods for producing a new pseudomonas lipase and protease and detergent washing compositions containing same
WO1994007984A1 (en) * 1992-09-25 1994-04-14 The Procter & Gamble Company Detergent composition comprising lime soap dispersant and lipase enzymes
WO1994007985A1 (en) * 1992-09-25 1994-04-14 The Procter & Gamble Company Detergent composition comprising lime soap dispersant and lipase enzymes
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
US5733763A (en) * 1988-08-19 1998-03-31 Novo Nordisk A/S Enzyme granulate formed of an enzyme-containing core and an enzyme-containing shell
US5772786A (en) * 1993-08-13 1998-06-30 The Procter & Gamble Company Detergent composition comprising lime soap dispersant and lipase enzymes
DE19954181A1 (de) * 1999-11-10 2001-05-31 Eppendorf Geraetebau Netheler Verwendung nichtionogener Tenside zur Durchführung enzymatischer Reaktionen
CN110804506A (zh) * 2019-11-13 2020-02-18 领先生物农业股份有限公司 一种微生物毛洗涤剂及其使用方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8629536D0 (en) * 1986-12-10 1987-01-21 Unilever Plc Enzymatic detergent composition
JPH0489897A (ja) * 1990-07-30 1992-03-24 Solvay Enzyme Prod Inc アルカリ性リパーゼを含む洗剤配合物
WO2011080835A1 (ja) 2009-12-28 2011-07-07 トヨタ自動車株式会社 車両用フード構造

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1930636A1 (de) * 1968-06-24 1970-07-09 Albright & Wilson Reinigungsmittel
DE1767413A1 (de) * 1967-08-14 1971-09-09 Henkel & Cie Gmbh Als Textilwaschmittel brauchbare Tensidkombinationen sowie diese enthaltende Wasch- oder Waschhilfsmittel
EP0130064A1 (de) * 1983-06-23 1985-01-02 Novo Nordisk A/S Enzymatischer Detergens-Zusatz, ein Detergens und ein Waschverfahren
EP0205208A2 (de) * 1985-06-11 1986-12-17 Unilever N.V. Enzymhaltiges Reinigungsmittel
EP0206390A2 (de) * 1985-06-11 1986-12-30 Unilever N.V. Enzymhaltiges Reinigungsmittel
EP0214761A2 (de) * 1985-08-07 1987-03-18 Novo Nordisk A/S Enzymatischer Reinigungsmittelzusatz, Reinigungsmittel und Waschverfahren

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8629536D0 (en) * 1986-12-10 1987-01-21 Unilever Plc Enzymatic detergent composition

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1767413A1 (de) * 1967-08-14 1971-09-09 Henkel & Cie Gmbh Als Textilwaschmittel brauchbare Tensidkombinationen sowie diese enthaltende Wasch- oder Waschhilfsmittel
DE1930636A1 (de) * 1968-06-24 1970-07-09 Albright & Wilson Reinigungsmittel
EP0130064A1 (de) * 1983-06-23 1985-01-02 Novo Nordisk A/S Enzymatischer Detergens-Zusatz, ein Detergens und ein Waschverfahren
EP0205208A2 (de) * 1985-06-11 1986-12-17 Unilever N.V. Enzymhaltiges Reinigungsmittel
EP0206390A2 (de) * 1985-06-11 1986-12-30 Unilever N.V. Enzymhaltiges Reinigungsmittel
EP0214761A2 (de) * 1985-08-07 1987-03-18 Novo Nordisk A/S Enzymatischer Reinigungsmittelzusatz, Reinigungsmittel und Waschverfahren

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4933287A (en) * 1985-08-09 1990-06-12 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
WO1989004361A1 (en) * 1987-11-02 1989-05-18 Novo-Nordisk A/S Enzymatic detergent composition
US5100796A (en) * 1988-02-22 1992-03-31 Synfina-Oleofina Methods for producing a new pseudomonas lipase and protease and detergent washing compositions containing same
US5733763A (en) * 1988-08-19 1998-03-31 Novo Nordisk A/S Enzyme granulate formed of an enzyme-containing core and an enzyme-containing shell
EP0368589A3 (de) * 1988-11-08 1990-10-31 Unilever Plc Enzymhaltige Waschmittel
EP0368589A2 (de) * 1988-11-08 1990-05-16 Unilever Plc Enzymhaltige Waschmittel
WO1990009440A1 (en) * 1989-02-20 1990-08-23 Novo Nordisk A/S Enzyme containing granulate and method for production thereof
US4950417A (en) * 1989-05-01 1990-08-21 Miles Inc. Detergent formulations containing alkaline lipase derived from Pseudomonas plantarii
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
EP0407225A1 (de) * 1989-07-07 1991-01-09 Unilever Plc Enzyme und enzymhaltiges Reinigungsmittel
WO1991000910A1 (en) * 1989-07-07 1991-01-24 Unilever Plc Enzymes and enzymatic detergent compositions
WO1994007985A1 (en) * 1992-09-25 1994-04-14 The Procter & Gamble Company Detergent composition comprising lime soap dispersant and lipase enzymes
WO1994007984A1 (en) * 1992-09-25 1994-04-14 The Procter & Gamble Company Detergent composition comprising lime soap dispersant and lipase enzymes
US5772786A (en) * 1993-08-13 1998-06-30 The Procter & Gamble Company Detergent composition comprising lime soap dispersant and lipase enzymes
DE19954181A1 (de) * 1999-11-10 2001-05-31 Eppendorf Geraetebau Netheler Verwendung nichtionogener Tenside zur Durchführung enzymatischer Reaktionen
CN110804506A (zh) * 2019-11-13 2020-02-18 领先生物农业股份有限公司 一种微生物毛洗涤剂及其使用方法
CN110804506B (zh) * 2019-11-13 2021-06-01 领先生物农业股份有限公司 一种微生物毛洗涤剂及其使用方法

Also Published As

Publication number Publication date
EP0271153B1 (de) 1990-03-14
AU605806B2 (en) 1991-01-24
ES2014465B3 (es) 1990-07-16
ZA879296B (en) 1989-08-30
BR8706682A (pt) 1988-07-19
JPS63161084A (ja) 1988-07-04
EP0271153A3 (en) 1988-08-10
CA1288368C (en) 1991-09-03
AU8222687A (en) 1988-06-16
GB8629535D0 (en) 1987-01-21
DE3761910D1 (de) 1990-04-19
JPH0696717B2 (ja) 1994-11-30

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