EP0232410A4 - Procede et tampon de lyse pour l'extraction d'un antigene de surface de l'hepatite b a partir de cellules de levure. - Google Patents

Procede et tampon de lyse pour l'extraction d'un antigene de surface de l'hepatite b a partir de cellules de levure.

Info

Publication number
EP0232410A4
EP0232410A4 EP19860905526 EP86905526A EP0232410A4 EP 0232410 A4 EP0232410 A4 EP 0232410A4 EP 19860905526 EP19860905526 EP 19860905526 EP 86905526 A EP86905526 A EP 86905526A EP 0232410 A4 EP0232410 A4 EP 0232410A4
Authority
EP
European Patent Office
Prior art keywords
hepatitis
extraction
buffer
yeast cells
surface antigen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19860905526
Other languages
German (de)
English (en)
Other versions
EP0232410A1 (fr
Inventor
Howard L Levine
Mark Levine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of EP0232410A1 publication Critical patent/EP0232410A1/fr
Publication of EP0232410A4 publication Critical patent/EP0232410A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/063—Lysis of microorganisms of yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP19860905526 1985-08-15 1986-08-15 Procede et tampon de lyse pour l'extraction d'un antigene de surface de l'hepatite b a partir de cellules de levure. Withdrawn EP0232410A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US76604385A 1985-08-15 1985-08-15
US766043 2001-01-19

Publications (2)

Publication Number Publication Date
EP0232410A1 EP0232410A1 (fr) 1987-08-19
EP0232410A4 true EP0232410A4 (fr) 1988-01-25

Family

ID=25075224

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19860905526 Withdrawn EP0232410A4 (fr) 1985-08-15 1986-08-15 Procede et tampon de lyse pour l'extraction d'un antigene de surface de l'hepatite b a partir de cellules de levure.

Country Status (3)

Country Link
EP (1) EP0232410A4 (fr)
JP (1) JPS63501121A (fr)
WO (1) WO1987001128A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE903626A (fr) * 1985-11-13 1986-03-03 Labofina Sa Procede pour la recuperation de polypeptides localises dans l'espace periplasmique de la levure.
RU1389060C (ru) * 1986-08-13 1993-06-30 Институт иммунологии Способ получени рекомбинатной вакцины к гепатиту В
US5026828A (en) * 1987-02-27 1991-06-25 Merck & Co., Inc. Method of purifying recombinant pres-1/S-2/S/S hepatitis B antigen from yeast
EP0280470B1 (fr) * 1987-02-27 1993-07-07 Merck & Co. Inc. Méthode pour la purification de l'antigène prés 1/S2/S de l'hépatite B à partir de levure
US5011915A (en) * 1987-10-26 1991-04-30 Merck & Co., Inc. Process for purifying recombinant hepatitis antigens
EP0314240A3 (fr) * 1987-10-26 1990-03-28 Merck & Co. Inc. Procédé de purification d'antigènes recombinants d'hépatite
CA1327851C (fr) * 1988-06-22 1994-03-15 Amy S. Christopher Imprimante reconfigurable
BE1009844A3 (fr) * 1995-12-22 1997-10-07 Genencor Int Procede de recolte d'une proteine.
KR100918098B1 (ko) * 2007-08-02 2009-09-24 대한민국 스트렙토마이세스 알부스 용균 추출물을 포함하는 백신 보좌제
CN105669826B (zh) * 2014-11-17 2021-02-26 苏州药明康德新药开发有限公司 酵母蛋白的提取试剂
JP6778867B2 (ja) * 2015-08-07 2020-11-04 国立大学法人山口大学 ポリペプチド抽出方法
CN111100795A (zh) * 2019-12-27 2020-05-05 深圳康泰生物制品股份有限公司 表达手足口病疫苗抗原的重组汉逊酵母细胞破碎缓冲液及其制备方法与应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0075395A2 (fr) * 1981-09-02 1983-03-30 Biogen, Inc. Produits montrant l'antigénéité de l'antigène E du virus de l'hépatite B et méthode de production de ces antigènes
EP0110385A2 (fr) * 1982-11-30 1984-06-13 THE UNITED STATES OF AMERICA as represented by the Secretary United States Department of Commerce Procédé de préparation de recombinants de poxvirus pour l'expression de gènes étrangers
EP0204680A2 (fr) * 1985-05-30 1986-12-10 Smithkline Biologicals S.A. Procédé d'isolement et de purification de l'antigène de surface de l'hépatite B

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3847737A (en) * 1965-03-22 1974-11-12 A Kanarek Inactivation of myxoviruses and method of preparing a vaccine therefrom
US4113712A (en) * 1976-03-08 1978-09-12 The Green Cross Corporation HBsAG Particle composed of single polypeptide subunits and the preparation procedure
FR2475572A1 (fr) * 1980-02-11 1981-08-14 Pasteur Institut Procede pour l'obtention de fragments de virus a enveloppe lipidique, en particulier d'antigenes utilisables comme vaccins, produits obtenus et applications
JPH0625069B2 (ja) * 1981-01-29 1994-04-06 ブリティッシュ・テクノロジー・グループ・リミテッド Ｂ型肝炎ワクチン製造方法
FR2549291B2 (fr) * 1982-10-29 1986-05-09 Radiotechnique Compelec Procede d'encapsulation de composants electroniques par extrusion de matiere plastique et applications a la fabrication de voyants lumineux et a l'encapsulation de circuits electroniques

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0075395A2 (fr) * 1981-09-02 1983-03-30 Biogen, Inc. Produits montrant l'antigénéité de l'antigène E du virus de l'hépatite B et méthode de production de ces antigènes
EP0110385A2 (fr) * 1982-11-30 1984-06-13 THE UNITED STATES OF AMERICA as represented by the Secretary United States Department of Commerce Procédé de préparation de recombinants de poxvirus pour l'expression de gènes étrangers
EP0204680A2 (fr) * 1985-05-30 1986-12-10 Smithkline Biologicals S.A. Procédé d'isolement et de purification de l'antigène de surface de l'hépatite B

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO8701128A1 *

Also Published As

Publication number Publication date
EP0232410A1 (fr) 1987-08-19
JPS63501121A (ja) 1988-04-28
WO1987001128A1 (fr) 1987-02-26

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH DE FR GB IT LI LU NL SE

17P Request for examination filed

Effective date: 19870813

A4 Supplementary search report drawn up and despatched

Effective date: 19880125

17Q First examination report despatched

Effective date: 19900821

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: AMGEN INC.

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 19920722

RIN1 Information on inventor provided before grant (corrected)

Inventor name: LEVINE, MARK

Inventor name: LEVINE, HOWARD, L.