EP0086813A1 - Dispositif de lavage et de rincage pour plaques de tritrage ou analogues. - Google Patents

Dispositif de lavage et de rincage pour plaques de tritrage ou analogues.

Info

Publication number
EP0086813A1
EP0086813A1 EP82902633A EP82902633A EP0086813A1 EP 0086813 A1 EP0086813 A1 EP 0086813A1 EP 82902633 A EP82902633 A EP 82902633A EP 82902633 A EP82902633 A EP 82902633A EP 0086813 A1 EP0086813 A1 EP 0086813A1
Authority
EP
European Patent Office
Prior art keywords
washing
plate
nozzles
rinsing
titer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP82902633A
Other languages
German (de)
English (en)
Other versions
EP0086813B1 (fr
Inventor
Eike Bohme
Peter Rubenzer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Prolic AG
Original Assignee
Prolic AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prolic AG filed Critical Prolic AG
Publication of EP0086813A1 publication Critical patent/EP0086813A1/fr
Application granted granted Critical
Publication of EP0086813B1 publication Critical patent/EP0086813B1/fr
Expired legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/02—Cleaning by the force of jets or sprays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L13/00—Cleaning or rinsing apparatus
    • B01L13/02—Cleaning or rinsing apparatus for receptacle or instruments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates

Definitions

  • the invention relates to a washing or rinsing device for titer plates or the like. with a plurality of cavities for receiving a medium to be examined or determined, for example antigens, with feed devices with nozzles for introducing a rinsing liquid into each of the cavities and with suction and / or blow-out devices to empty liquid from the cavities and with guide devices that move the titer plate to the wash station and hold it there precisely.
  • Elisa method applied (enzyme linked itranunosorbent assay), or the various methods of this system.
  • antibodies are absorbed on the titer plate in a first manufacturing step, then the titer plate is washed, then in a further process step the addition of a residual solution containing the antigen is carried out, followed by washing , whereupon enzyme-labeled specific antibodies are added to the titer plate, then the titer plate is washed again, after which the enzyme substrate is added.
  • the actual measurement is carried out in a phonometer.
  • washing the titer plates is an essential part of
  • OMPI is part of the process, which on the one hand is relatively time-consuming and on the other hand has the disadvantage that very toxic, ie extremely disease-causing, living matter must be handled very often, which makes the manual handling of the titer plate undesirable.
  • the titer plates currently in use have 96 cavities, each of which corresponds practically to a cuvette. During the method or during the determination or determination of the antigens or the antibodies, there are samples in each cavity (for example samples from different patients). It must now be absolutely prevented that during the
  • the object of the invention is therefore to provide a washing or
  • a washing plate provided with nozzles, to which the titer plate to be rinsed is sealed by means of a guide device can be brought and on which the titer plate is held under pressure during the washing or rinsing process, each cavity of the titer plate being assigned a nozzle and an annular gap which connects the cavity to a drainage channel, and each individual cavity being sealed off from the other cavities.
  • Automatic washing machines used as washing or rinsing liquid tap water. Compressed air is used as the drying medium.
  • the gaseous medium is pulsed through the nozzles, whereby the
  • An embodiment of the invention provides that the washing plate is located in a sealed washing chamber.
  • a sensor can be arranged in the washing chamber, which checks the seal between the washing plate and the titer plates to be cleaned and possibly triggers a pre-programmed self-cleaning process for the washing plate after a leak has been detected, so that the subsequent titer plates are not contaminated.
  • Such self-cleaning can, however, also be carried out profile-specifically after passing through a predetermined number of titer plates to be cleaned. This can be done through the wash plate on the opposite side of the nozzle.
  • a device for lifting the washing plate from the titer plate can also be provided, with another one after the liquid has passed through the nozzles in the washing plate
  • the washing or rinsing machine according to the invention advantageously has guide devices which keep the titer plates free of soil, ie the titer plate is guided along its sides or along its lower side edges, so that there is no contamination on the underside of the plate. This is important for the subsequent measurement of the samples.
  • Another embodiment of the invention provides a tilting device for the titer plate, preferably in the washing area. The titer plate can be tilted together with the wash plate. The advantage of this design is that the rinsing liquid can run downwards and, under certain circumstances, no separate drying process using compressed air is necessary.
  • FIG. 1 schematically shows a diagram of a titer plate to be cleaned
  • FIG. 2 likewise schematically shows the washing or dishwashing machine according to the invention
  • FIG. 3 shows a plan view of the washing plate according to the invention
  • FIG. 3a shows a section along line II of FIG. 3 with a titer plate applied
  • FIGS. 4 to 6 show sections through the wash plate with different nozzle shapes
  • FIG. 7 shows schematically a section through a wash plate
  • FIG. 8 shows a cross section through a further exemplary embodiment a washing plate along the line II of Fig. 3
  • Fig. 9 shows a same section in a further embodiment of a washing plate with articulated titer plate. Description of preferred embodiments
  • FIG. 1 A titer plate 1 with cavities 2 is shown in FIG. 1.
  • the titer plates 1 which are in use today, there are generally eight rows, each with twelve cavities 2, which gives a total of ninety-six.
  • the washing or rinsing machine has a stacking device 3, a washing point 6 and a stacking device 4.
  • the titer plates 1 to be washed or rinsed are placed, for example, in a stack of ten in the stacking device 3, brought individually by a conveyor device which has toothed belts 7 in the direction of arrow V to the washing point below the washing plate 5 and come after rinsing into the stacking device 4.
  • the titer plates 1 come to rest on a support plate 8, which has a centering device 9, for example centering bolts, for the exact positioning of the titer plate 1.
  • the support plate 8 is raised by means of the lifting device 10, which is, for example, a pneumatic cylinder, and the titer plate 1 is pressed onto the washing plate 5.
  • the lifting device 10 which is, for example, a pneumatic cylinder
  • the washing plate 5 has a plurality of nozzles 11, the number and position of which corresponds exactly to the number and position of the cavities 2 in the titer plate 1.
  • the washing plate 5 has ninety-six nozzles 11.
  • each cavity 2 is assigned a nozzle 11, as can be seen from FIG. 3a.
  • a first operation rinsing medium for example water
  • a first operation rinsing medium for example water
  • the nozzle heads 11 'of the nozzles 11 are designed such that they have a decreasing cross-section from front to back.
  • FIGS. 1-10 Various exemplary embodiments of the nozzle heads 11 * are shown in FIGS.
  • the liquid medium supply is switched off and compressed air is pressed into the cavities 2 through the same inlets E and the same nozzles 11.
  • the humidity and the temperature of the compressed air can be adjusted or regulated.
  • the titer plate can be lowered again by means of the support plate 8 and fed to the stacking device 4 by means of the toothed belt 7.
  • the titer plate 1 is only slightly lowered from the washing plate 5, so that only a small gap is formed between the top surface 1 * of the titer plate 1 and the sealing plate 14 of the washing plate 5. If rinsing medium is pressed through the nozzles 11 again, the sealing plate 14 of the titer plate 1 is also cleaned and the top surface of the washing plate 5 is cleaned. Such a rinsing is expediently carried out before the drying cycle, i.e. before supplying compressed air as described above.
  • the titer plate 1 can now be pressed against the washing plate 5 again by means of the support plate 8, whereupon the compressed air is now supplied and the titer plate 1 is dried.
  • a sealing plate 14 made of plastic material is provided on the washing plate 5.
  • the washing plate 5 is divided into a fixed upper part 5a which carries the nozzles 11 and a movable lower part 5b.
  • the upper part 5a also contains the antechamber 16 or the feed lines 15.
  • the movable lower part 5b has bores which are concentric with the nozzles 11 and which form the annular gap 12 with the nozzles 11.
  • the nozzles 11 protrude through the bores of the lower part 5b.
  • the nozzles 11 protrude through the bores of the lower part 5b.
  • the nozzles 11 are elongated compared to the exemplary embodiments described above, and at the start of the washing process or immediately before, the lower part 5b is lowered to the lower end of the nozzles 11.
  • the titer plate 1 When pressing up, the titer plate 1 initially seals with the lower part 5b of the washing plate 5. Then
  • the lower part 5b is brought together with the titer plate 1 to the upper part 5a.
  • the nozzles 11 penetrate into the cavities 2 of the titer plate 1 and partially displace the liquid to be emptied in the direction of the drainage channels 13 until the lower part 5b lies closely against the upper part 5a.
  • the system is now closed and the rinsing process can begin.
  • the concentric bores for each annular gap 12 are designed in terms of volume so that the liquid displaced by the immersion of the nozzle 11 is received in the concentric bores (annular gap 12) and contamination is thereby avoided.
  • the volume of the cavity 2 to be rinsed or dried is reduced by the preferably cylindrical nozzle 11 and its immersion in the cavity 2. Two thirds of the nozzles 11 preferably dip into the cavities 2. The resulting higher flow speed increases the cleaning and drying efficiency.
  • the washing plate 5 can either be provided with H-shaped feed channels 15, as shown in FIG. 7, the inlet E being arranged in the middle of the connecting web, or it As shown in FIG. 1, the washing plate 5 can have one or more antechambers 16 with an inclined wall 16 ', so that the chamber cross section, seen from the inlet E for the flushing medium, decreases from row of nozzles to row of nozzles.
  • the air, water and power connections are advantageously located on the rear of the device, and built-in pressure reducing valves and pressure gauges for pressure control for the flushing medium and compressed air are provided.
  • the toothed belt 7 is advantageously driven by a DC motor.
  • the washing plate 5 is advantageously exchangeable by means of a quick-release fastener.
  • the device is controlled by a microprocessor. The times for washing, dwell and drying processes can be freely selected, and the sequence and the repetition cycles can also be freely selected.
  • Control systems are provided for checking for empty stackers, checking for available space in the stackers, checking for correct pressure conditions in the device and checking for leaks in the system.
  • the messages are given by acoustic signals and / or a corresponding LED display.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Cleaning By Liquid Or Steam (AREA)

Abstract

Le dispositif de lavage ou de rinçage pour plaques de titrage (1) comporte une plaque de lavage (5) munie de buses (11) en nombre égal à celui des cavités (2) de la plaque de titrage (1). Durant l'opération de lavage la plaque de titrage (1) est maintenue de manière étanche contre la plaque de lavage (5) de façon à éviter de salir les cavités adjacentes. Des canaux d'évacuation (13) sont prévus dans la plaque de lavage (5).
EP82902633A 1981-08-31 1982-08-30 Dispositif de lavage et de rincage pour plaques de tritrage ou analogues Expired EP0086813B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT0375881A AT375841B (de) 1981-08-31 1981-08-31 Wasch- bzw. spuelvorrichtung fuer titerplatten od.dgl.
AT3758/81 1981-08-31

Publications (2)

Publication Number Publication Date
EP0086813A1 true EP0086813A1 (fr) 1983-08-31
EP0086813B1 EP0086813B1 (fr) 1986-04-23

Family

ID=3555020

Family Applications (1)

Application Number Title Priority Date Filing Date
EP82902633A Expired EP0086813B1 (fr) 1981-08-31 1982-08-30 Dispositif de lavage et de rincage pour plaques de tritrage ou analogues

Country Status (6)

Country Link
US (1) US4559664A (fr)
EP (1) EP0086813B1 (fr)
JP (1) JPS58501594A (fr)
AT (1) AT375841B (fr)
DE (1) DE3270768D1 (fr)
WO (1) WO1983000819A1 (fr)

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DE8905294U1 (de) * 1989-04-26 1990-05-23 Fa. Andreas Hettich, 7200 Tuttlingen Vorrichtung zum Füllen und Entleeren von Probenröhrchen
DE4024932A1 (de) * 1990-08-06 1992-02-20 Henning Berlin Gmbh Verfahren zum waschen von traegern mit immobilisierten reaktionsprodukten oder reaktionspartnern, die bei immundiagnostischen bestimmungsverfahren gebildet oder verwendet werden, sowie vorrichtung zur durchfuehrung dieses verfahrens
US5402734A (en) * 1992-12-11 1995-04-04 Melles Griot, Inc. Optical table having debris retention structures
US5807359A (en) 1993-06-08 1998-09-15 Bemis Manufacturing Company Medical suction system
US6244311B1 (en) 1994-12-29 2001-06-12 Bemis Manufacturing Company Method and apparatus for removing and disposing of body fluids
US6358232B1 (en) 1994-12-29 2002-03-19 Bemis Manufacturing Company Method and apparatus for removing and disposing of body fluids
US5620894A (en) * 1995-06-16 1997-04-15 Glaxo Wellcome Inc. Apparatus for automated biological cell harvesting
US5730938A (en) * 1995-08-09 1998-03-24 Bio-Chem Laboratory Systems, Inc. Chemistry analyzer
DE19534521C1 (de) * 1995-09-06 1996-11-21 Atotech Deutschland Gmbh Verfahren und Vorrichtung zum Behandeln von sich in Werkstücke erstreckende Löcher oder Vertiefungen mit flüssigen Behandlungsmitteln und Anwendung des Verfahrens zur Behandlung von Leiterplatten
JP3419431B2 (ja) * 1996-08-21 2003-06-23 日本電子株式会社 生化学自動分析装置における洗浄装置
US5882597A (en) * 1997-09-22 1999-03-16 Astle; Thomas W. Bioassay plate washer having concentric aspirate and wash needles
US5951783A (en) * 1998-05-15 1999-09-14 Bio-Tek Holdings, Inc. Universal washing apparatus for microtiter plate and the like
WO2001072350A1 (fr) 2000-03-28 2001-10-04 Bemis Manufacturing Company Instrument d'aspiration medical et procedes de vidange de l'instrument
US7674248B2 (en) 2000-03-28 2010-03-09 Bemis Manufacturing Company Medical suction apparatus and methods for draining same
US7585292B2 (en) 2000-03-28 2009-09-08 Bemis Manufacturing Company Medical suction apparatus and draining of same
WO2002055134A2 (fr) 2001-01-12 2002-07-18 Bemis Mfg Co Procede et appareil destines a l'elimination de liquides organiques contenus dans un recipient
US7516749B2 (en) * 2003-04-08 2009-04-14 Neogen, Llc Methods of and apparatus for washing high-density microplates
EP1617951A4 (fr) * 2003-04-08 2006-08-02 Irm Llc Dispositifs, systemes et procedes de retrait et de distribution de matieres
ATE393870T1 (de) * 2005-01-25 2008-05-15 Gas Turbine Efficiency Ab Sondenreinigungsverfahren und -vorrichtung
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CA2769552A1 (fr) * 2009-07-31 2011-02-03 Simon Stafford Moyen pour manipulation de liquide amelioree dans une microplaque
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Also Published As

Publication number Publication date
DE3270768D1 (en) 1986-05-28
US4559664A (en) 1985-12-24
JPS58501594A (ja) 1983-09-22
AT375841B (de) 1984-09-10
ATA375881A (de) 1984-02-15
JPH032264B2 (fr) 1991-01-14
WO1983000819A1 (fr) 1983-03-17
EP0086813B1 (fr) 1986-04-23

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