EA201791724A1 - Способ получения гонадотропина - Google Patents

Способ получения гонадотропина

Info

Publication number
EA201791724A1
EA201791724A1 EA201791724A EA201791724A EA201791724A1 EA 201791724 A1 EA201791724 A1 EA 201791724A1 EA 201791724 A EA201791724 A EA 201791724A EA 201791724 A EA201791724 A EA 201791724A EA 201791724 A1 EA201791724 A1 EA 201791724A1
Authority
EA
Eurasian Patent Office
Prior art keywords
gonadotropin
obtaining
host cell
hcg
encodes
Prior art date
Application number
EA201791724A
Other languages
English (en)
Other versions
EA034022B1 (ru
Inventor
Даниель Плаксин
Айелет Гринхют
Original Assignee
Ферринг Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ферринг Б.В. filed Critical Ферринг Б.В.
Publication of EA201791724A1 publication Critical patent/EA201791724A1/ru
Publication of EA034022B1 publication Critical patent/EA034022B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/06—Drugs for disorders of the endocrine system of the anterior pituitary hormones, e.g. TSH, ACTH, FSH, LH, PRL, GH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99004—Beta-galactoside alpha-2,3-sialyltransferase (2.4.99.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Reproductive Health (AREA)
  • Toxicology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

Клетка-хозяин, отличающаяся тем, что она содержит интегрированную в ее геном последовательность, кодирующую α-цепь ХГЧ (хорионического гонадотропина человека), и применение клетки-хозяина для получения рекомбинантного ХГЧ.
EA201791724A 2015-04-24 2016-04-22 Способ получения гонадотропина EA034022B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15164965 2015-04-24
PCT/EP2016/059006 WO2016170113A1 (en) 2015-04-24 2016-04-22 Method of production of gonadotrophin

Publications (2)

Publication Number Publication Date
EA201791724A1 true EA201791724A1 (ru) 2018-04-30
EA034022B1 EA034022B1 (ru) 2019-12-19

Family

ID=53002568

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201791724A EA034022B1 (ru) 2015-04-24 2016-04-22 Способ получения гонадотропина

Country Status (32)

Country Link
US (1) US10464984B2 (ru)
EP (1) EP3286209B1 (ru)
JP (1) JP6964519B2 (ru)
KR (1) KR102658666B1 (ru)
CN (2) CN107532172A (ru)
AU (1) AU2016251314B2 (ru)
BR (1) BR112017016895A2 (ru)
CA (1) CA2975449A1 (ru)
CL (1) CL2017002661A1 (ru)
CO (1) CO2017011964A2 (ru)
DK (1) DK3286209T3 (ru)
EA (1) EA034022B1 (ru)
ES (1) ES2837111T3 (ru)
HK (1) HK1246325A1 (ru)
HR (1) HRP20201945T1 (ru)
HU (1) HUE051879T2 (ru)
IL (1) IL253694B (ru)
LT (1) LT3286209T (ru)
MA (1) MA41949B1 (ru)
MD (1) MD3286209T2 (ru)
MX (1) MX2017010884A (ru)
MY (1) MY185823A (ru)
PH (1) PH12017501585A1 (ru)
PT (1) PT3286209T (ru)
RS (1) RS61163B1 (ru)
SA (1) SA517390029B1 (ru)
SG (1) SG11201706832PA (ru)
SI (1) SI3286209T1 (ru)
TN (1) TN2017000328A1 (ru)
UA (1) UA124187C2 (ru)
WO (1) WO2016170113A1 (ru)
ZA (1) ZA201707136B (ru)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JOP20200034A1 (ar) 2017-09-01 2020-02-13 Ferring Bv تركيبة للتنشيط المتحكم فيه للمبيض
CN108264549A (zh) * 2018-03-29 2018-07-10 北京伟杰信生物科技有限公司 重组绒促性素rhCG的制备方法及应用
JP2021522268A (ja) 2018-04-30 2021-08-30 フェリング ベスローテン フェンノートシャップ 制御された卵巣刺激のための組成物
CN110812366B (zh) * 2019-11-18 2023-11-17 珠海丽凡达生物技术有限公司 一种可用于激素补充的mRNA药物及其制备方法
CN113337562B (zh) * 2021-05-24 2022-06-03 宁波人健药业集团股份有限公司 使用CHO细胞高效发酵生产rhCG的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6855544B1 (en) 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
WO2001058493A1 (en) * 2000-02-11 2001-08-16 Maxygen Aps Conjugates of follicle stimulating hormones
US20030036181A1 (en) 2000-06-30 2003-02-20 Okkels Jens Sigurd Peptide extended glycosylated polypeptides
CN1194016C (zh) * 2000-12-29 2005-03-23 申庆祥 新的人绒毛膜促性腺激素－促黄体素融合蛋白及其制法和用途
CN101250531B (zh) * 2006-11-27 2013-04-24 株式会社Lg生命科学 一核苷酸序列、包含该序列的表达载体、该载体转化的动物细胞及利用该细胞生产人fsh的方法
TWI532495B (zh) 2009-10-05 2016-05-11 菲瑞茵國際中心股份有限公司 藥學製劑
US8326547B2 (en) * 2009-10-07 2012-12-04 Nanjingjinsirui Science & Technology Biology Corp. Method of sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm
EP2516664B1 (en) * 2009-12-21 2015-07-22 Pharmathene Inc. Recombinant butyrylcholinesterases and truncates thereof
CA2868391A1 (en) * 2012-04-02 2013-10-10 Stephane Bancel Polynucleotides comprising n1-methyl-pseudouridine and methods for preparing the same
WO2013151664A1 (en) 2012-04-02 2013-10-10 modeRNA Therapeutics Modified polynucleotides for the production of proteins
CN103539862B (zh) * 2013-11-01 2015-04-01 广州联康生物科技有限公司 一种长效重组促卵泡激素及其应用

Also Published As

Publication number Publication date
ZA201707136B (en) 2022-03-30
HUE051879T2 (hu) 2021-03-29
UA124187C2 (uk) 2021-08-04
IL253694A0 (en) 2017-09-28
MD3286209T2 (ro) 2021-04-30
PH12017501585A1 (en) 2018-02-05
SI3286209T1 (sl) 2021-01-29
US20180127477A1 (en) 2018-05-10
ES2837111T3 (es) 2021-06-29
AU2016251314B2 (en) 2019-12-05
MX2017010884A (es) 2017-12-07
BR112017016895A2 (pt) 2018-03-27
MA41949A (fr) 2018-02-28
TN2017000328A1 (en) 2019-01-16
CN107532172A (zh) 2018-01-02
LT3286209T (lt) 2021-02-25
CL2017002661A1 (es) 2018-03-23
AU2016251314A1 (en) 2017-08-17
IL253694B (en) 2021-09-30
MY185823A (en) 2021-06-11
SG11201706832PA (en) 2017-09-28
KR102658666B1 (ko) 2024-04-17
US10464984B2 (en) 2019-11-05
SA517390029B1 (ar) 2021-04-04
HK1246325A1 (zh) 2018-09-07
EP3286209A1 (en) 2018-02-28
RS61163B1 (sr) 2021-01-29
EA034022B1 (ru) 2019-12-19
JP6964519B2 (ja) 2021-11-10
WO2016170113A1 (en) 2016-10-27
CN114107315A (zh) 2022-03-01
MA41949B1 (fr) 2020-12-31
PT3286209T (pt) 2020-12-04
CO2017011964A2 (es) 2018-03-09
HRP20201945T1 (hr) 2021-02-05
EP3286209B1 (en) 2020-11-25
DK3286209T3 (da) 2020-12-21
KR20170138390A (ko) 2017-12-15
CA2975449A1 (en) 2016-10-27
JP2018512885A (ja) 2018-05-24

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