EA200100287A1 - Высокоаффинные антитела - Google Patents
Высокоаффинные антителаInfo
- Publication number
- EA200100287A1 EA200100287A1 EA200100287A EA200100287A EA200100287A1 EA 200100287 A1 EA200100287 A1 EA 200100287A1 EA 200100287 A EA200100287 A EA 200100287A EA 200100287 A EA200100287 A EA 200100287A EA 200100287 A1 EA200100287 A1 EA 200100287A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- samples
- antibody
- incubating
- hour
- sample
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
- C07K16/3007—Carcino-embryonic Antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
- A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
- A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
- A61K47/6811—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being a protein or peptide, e.g. transferrin or bleomycin
- A61K47/6815—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Oncology (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Высокоаффинное моноклональное антитело, причем аффинность отличается следующим:(i) инкубацией первого и второго образцов антитела в лунках покрытого антигеном микротитровального планшета при концентрации, выбираемой из пределов участка линейной зависимости в калибровочной кривой, при pH 7,2 в течение 1 ч при 37°C;(ii) удалением несвязанного антитела из обоих образцов;(iii) инкубацией первого образца в ФСБ при pH 7,2 в течение 1 ч при 37°C и снижение pH у второго образца до pH 3 или ниже и инкубацией в течение 1 ч при 37°C;(iv) удалением несвязанного антитела из обоих образцов;(v) инкубацией обоих образцов с конъюгатом анти-антитела и щелочной фосфатазы в течение 1 ч при 37°C;(vi) удалением несвязанного конъюгата из обоих образцов; и(vii) добавлением к образцам субстрата PNPP, измерением поглощения образцов при 405 нм и определением количества антитела, связанного с антигеном, причем связанное количество во втором образце более чем на 50% больше такового в первом образце.Международная заявка была опубликована вместе с отчетом о международном поиске.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9818915.2A GB9818915D0 (en) | 1998-08-28 | 1998-08-28 | Antibodies |
| PCT/GB1999/002729 WO2000012556A1 (en) | 1998-08-28 | 1999-08-20 | High-affinity antibodies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EA200100287A1 true EA200100287A1 (ru) | 2001-08-27 |
Family
ID=10838074
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA200100287A EA200100287A1 (ru) | 1998-08-28 | 1999-08-20 | Высокоаффинные антитела |
Country Status (21)
| Country | Link |
|---|---|
| EP (1) | EP1107990A1 (ru) |
| JP (1) | JP2002525082A (ru) |
| KR (1) | KR20010089174A (ru) |
| CN (1) | CN1315966A (ru) |
| AU (1) | AU5435099A (ru) |
| BG (1) | BG105294A (ru) |
| BR (1) | BR9913303A (ru) |
| CA (1) | CA2340865A1 (ru) |
| EA (1) | EA200100287A1 (ru) |
| GB (1) | GB9818915D0 (ru) |
| HR (1) | HRP20010149A2 (ru) |
| HU (1) | HUP0103233A3 (ru) |
| ID (1) | ID28905A (ru) |
| IL (1) | IL141523A0 (ru) |
| MX (1) | MXPA01002248A (ru) |
| NO (1) | NO20011006D0 (ru) |
| PL (1) | PL346472A1 (ru) |
| TR (1) | TR200100643T2 (ru) |
| WO (1) | WO2000012556A1 (ru) |
| YU (1) | YU15401A (ru) |
| ZA (1) | ZA200101573B (ru) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0031284D0 (en) * | 2000-12-21 | 2001-01-31 | Ks Biomedix Ltd | High affinity antibodies |
| GB0112844D0 (en) * | 2001-05-25 | 2001-07-18 | Psimei Pharmaceuticals Plc | Neutron capture therapy |
| AU2003256266A1 (en) * | 2002-06-12 | 2003-12-31 | Genencor International, Inc. | Methods and compositions for milieu-dependent binding of a targeted agent to a target |
| US20030232401A1 (en) * | 2002-06-12 | 2003-12-18 | Pugia Michael J. | Bacterial test method by glycated label binding |
| WO2005058236A2 (en) * | 2003-12-12 | 2005-06-30 | Genencor International, Inc. | Cab molecules |
| CN111848790B (zh) * | 2020-08-07 | 2022-02-22 | 上海交通大学 | 一种牛源抗金黄色葡萄球菌的单链抗体及其制备和应用 |
| CN112724255A (zh) * | 2021-01-28 | 2021-04-30 | 成都金昆生物科技有限公司 | 靶向癌胚抗原的小分子抗体 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5081235A (en) * | 1989-07-26 | 1992-01-14 | City Of Hope | Chimeric anti-cea antibody |
| GB9014932D0 (en) * | 1990-07-05 | 1990-08-22 | Celltech Ltd | Recombinant dna product and method |
-
1998
- 1998-08-28 GB GBGB9818915.2A patent/GB9818915D0/en not_active Ceased
-
1999
- 1999-08-20 CN CN99810367A patent/CN1315966A/zh active Pending
- 1999-08-20 BR BR9913303-2A patent/BR9913303A/pt not_active IP Right Cessation
- 1999-08-20 WO PCT/GB1999/002729 patent/WO2000012556A1/en not_active Application Discontinuation
- 1999-08-20 JP JP2000571062A patent/JP2002525082A/ja active Pending
- 1999-08-20 IL IL14152399A patent/IL141523A0/xx unknown
- 1999-08-20 CA CA002340865A patent/CA2340865A1/en not_active Abandoned
- 1999-08-20 YU YU15401A patent/YU15401A/sh unknown
- 1999-08-20 ID IDW20010714A patent/ID28905A/id unknown
- 1999-08-20 EP EP99940355A patent/EP1107990A1/en not_active Withdrawn
- 1999-08-20 MX MXPA01002248A patent/MXPA01002248A/es unknown
- 1999-08-20 HU HU0103233A patent/HUP0103233A3/hu unknown
- 1999-08-20 PL PL99346472A patent/PL346472A1/xx not_active Application Discontinuation
- 1999-08-20 KR KR1020017002434A patent/KR20010089174A/ko not_active Application Discontinuation
- 1999-08-20 TR TR2001/00643T patent/TR200100643T2/xx unknown
- 1999-08-20 EA EA200100287A patent/EA200100287A1/ru unknown
- 1999-08-20 AU AU54350/99A patent/AU5435099A/en not_active Abandoned
-
2001
- 2001-02-26 BG BG105294A patent/BG105294A/bg unknown
- 2001-02-26 ZA ZA200101573A patent/ZA200101573B/en unknown
- 2001-02-27 NO NO20011006A patent/NO20011006D0/no not_active Application Discontinuation
- 2001-02-28 HR HR20010149A patent/HRP20010149A2/hr not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| CN1315966A (zh) | 2001-10-03 |
| HUP0103233A3 (en) | 2003-10-28 |
| BG105294A (bg) | 2001-12-29 |
| TR200100643T2 (tr) | 2001-07-23 |
| NO20011006L (no) | 2001-02-27 |
| HRP20010149A2 (en) | 2002-02-28 |
| IL141523A0 (en) | 2002-03-10 |
| JP2002525082A (ja) | 2002-08-13 |
| EP1107990A1 (en) | 2001-06-20 |
| YU15401A (sh) | 2003-07-07 |
| NO20011006D0 (no) | 2001-02-27 |
| ZA200101573B (en) | 2002-02-26 |
| MXPA01002248A (es) | 2002-05-08 |
| PL346472A1 (en) | 2002-02-11 |
| BR9913303A (pt) | 2001-10-09 |
| AU5435099A (en) | 2000-03-21 |
| CA2340865A1 (en) | 2000-03-09 |
| WO2000012556A1 (en) | 2000-03-09 |
| KR20010089174A (ko) | 2001-09-29 |
| ID28905A (id) | 2001-07-12 |
| GB9818915D0 (en) | 1998-10-21 |
| HUP0103233A2 (hu) | 2001-12-28 |
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