EA038931B9 - Композиции и способы получения полипептидов с модифицированным профилем гликозилирования в клетках растений - Google Patents

Композиции и способы получения полипептидов с модифицированным профилем гликозилирования в клетках растений

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Publication number
EA038931B9
EA038931B9 EA201791059A EA201791059A EA038931B9 EA 038931 B9 EA038931 B9 EA 038931B9 EA 201791059 A EA201791059 A EA 201791059A EA 201791059 A EA201791059 A EA 201791059A EA 038931 B9 EA038931 B9 EA 038931B9
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Prior art keywords
glycosylation pattern
plant
compositions
methods
plant cells
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EA201791059A
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English (en)
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EA201791059A1 (ru
EA038931B1 (ru
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Одед Шосейов
Хелена Магриссо
Цви Цвирин
Амит Яари
Original Assignee
Йиссум Рисерч Дивелопмент Компани Оф Зе Хебрю Юниверсити Оф Иерусалим Лтд.
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Publication of EA201791059A1 publication Critical patent/EA201791059A1/ru
Publication of EA038931B1 publication Critical patent/EA038931B1/ru
Publication of EA038931B9 publication Critical patent/EA038931B9/ru

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    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
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    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion

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Abstract

Предложен способ модификации профиля гликозилирования полипептида, представляющего интерес, в растении или клетке растения. Указанный способ включает экспрессию в растении или клетке растения, трансформированных для экспрессии во внутриклеточном компартменте по меньшей мере одной гликозидазы, последовательности нуклеиновой кислоты, которая кодирует полипептид, представляющий интерес, таким образом, что по меньшей мере одна гликозидаза и полипептид, представляющий интерес, являются совместно локализованными во внутриклеточном компартменте растения или клетки растения, посредством чего модифицируется профиль гликозилирования полипептида, представляющего интерес, в растении или клетке растения.
EA201791059A 2014-11-20 2015-11-19 Композиции и способы получения полипептидов с модифицированным профилем гликозилирования в клетках растений EA038931B9 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462082204P 2014-11-20 2014-11-20
PCT/IL2015/051112 WO2016079739A2 (en) 2014-11-20 2015-11-19 Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells

Publications (3)

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EA201791059A1 EA201791059A1 (ru) 2018-02-28
EA038931B1 EA038931B1 (ru) 2021-11-11
EA038931B9 true EA038931B9 (ru) 2022-02-18

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US (3) US20170283822A1 (ru)
EP (1) EP3221455B1 (ru)
CN (1) CN107810271B (ru)
BR (1) BR112017010555A2 (ru)
EA (1) EA038931B9 (ru)
IL (1) IL252389B (ru)
WO (1) WO2016079739A2 (ru)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3548612A4 (en) 2016-12-01 2020-04-15 Plantform Corporation TRANSGENIC PLANT WITH REDUCED FUCOSYL TRANSFERASE AND XYLOSYL TRANSFERASE ACTIVITY
CN110092831B (zh) * 2018-01-30 2022-05-20 北京睿诚海汇健康科技有限公司 植物作为宿主在表达阿达木抗体中的应用
WO2019170899A1 (en) * 2018-03-08 2019-09-12 Targimmune Therapeutics Ag A vector for the expression of cetuximab antibody chains
MX2021001016A (es) * 2018-08-17 2021-04-19 Modern Meadow Inc Proteinas de fusion para hidroxilar aminoacidos y productos.
KR102317403B1 (ko) * 2019-01-28 2021-10-29 주식회사 바이오앱 당화된 Ag85A 단백질을 포함하는 결핵 예방용 백신 조성물 및 이의 제조방법
WO2021101351A2 (ko) * 2019-11-21 2021-05-27 (주)지플러스 생명과학 비푸코실화된 담배를 이용하여 생산한 항체 및 이의 용도
KR102461600B1 (ko) * 2020-07-21 2022-11-02 주식회사 피토맵 N-당질화 돌연변이 벼, 이의 제조방법 및 이를 이용한 단백질 생산용 벼의 제조방법
KR20240066509A (ko) * 2022-11-02 2024-05-16 주식회사 피토맵 N-당질화 돌연변이 벼를 이용한 단일클론항체 생산용 벼의 제조방법 및 이에 의해 제조된 단일클론항체

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
CA1192510A (en) 1981-05-27 1985-08-27 Lawrence E. Pelcher Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom
JPS6054684A (ja) 1983-09-05 1985-03-29 Teijin Ltd 新規ｄνａ及びハイブリツドｄνａ
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
CA1288073C (en) 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
GB8608850D0 (en) 1986-04-11 1986-05-14 Diatech Ltd Packaging system
JPS6314693A (ja) 1986-07-04 1988-01-21 Sumitomo Chem Co Ltd 植物ウイルスｒｎａベクタ−
ES2060646T3 (es) 1987-02-09 1994-12-01 Lubrizol Genetics Inc Virus rna hibrido.
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5693507A (en) 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5360726A (en) 1989-09-12 1994-11-01 Board Of Trustees Operating Michigan State University Polypeptides enabling sorting of proteins to vacuoles in plants
US5202422A (en) * 1989-10-27 1993-04-13 The Scripps Research Institute Compositions containing plant-produced glycopolypeptide multimers, multimeric proteins and method of their use
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
US5849870A (en) 1993-03-25 1998-12-15 Novartis Finance Corporation Pesticidal proteins and strains
US5496934A (en) * 1993-04-14 1996-03-05 Yissum Research Development Company Of The Hebrew University Of Jerusalem Nucleic acids encoding a cellulose binding domain
JP4052605B2 (ja) 1994-05-18 2008-02-27 タカラバイオ株式会社 α−１，３／４−フコシダーゼ遺伝子
EP0833928A1 (en) 1995-06-23 1998-04-08 Danisco Ingredients A/S (Danisco A/S) Novel beta-xylosidase, nucleotide sequence encoding it, and use thereof
IL155588A0 (en) * 2003-04-27 2003-11-23 Metabogal Ltd Methods for expression of enzymatically active recombinant lysosomal enzymes in transgenic plant root cells and vectors used thereby
US20050032211A1 (en) 1996-09-26 2005-02-10 Metabogal Ltd. Cell/tissue culturing device, system and method
JP2002524023A (ja) * 1997-08-07 2002-08-06 オーバーン・ユニバーシティ 万能葉緑体組込および発現ベクター、その形質転換植物ならびに生産物
DE19900635A1 (de) * 1999-01-11 2000-07-13 Deutsches Krebsforsch Selektion von monoklonalen Antikörpern
US6331416B1 (en) * 1999-06-10 2001-12-18 Cbd Technologies Ltd. Process of expressing and isolating recombinant proteins and recombinant protein products from plants, plant derived tissues or cultured plant cells
EP1224309A2 (en) 1999-10-21 2002-07-24 Monsanto Company Post-translational modification of recombinant proteins produced in plants
US20030159178A1 (en) 2000-02-10 2003-08-21 Peter Ulvskov Method for remodelling cell wall polysaccharide structures in plants
US6391683B1 (en) 2000-06-21 2002-05-21 Siliconware Precision Industries Co., Ltd. Flip-chip semiconductor package structure and process for fabricating the same
US7625756B2 (en) * 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US7449308B2 (en) * 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US20090203079A1 (en) * 2000-10-20 2009-08-13 Board Of Trustees Of Michigan State University Transgenic monocot plants encoding beta-glucosidase and xylanase
JP4532118B2 (ja) * 2002-03-19 2010-08-25 スティヒティング ディーンスト ランドバウクンディフ オンデルズーク 植物体内のグリカンプロセシングの最適化
AR042145A1 (es) 2002-11-27 2005-06-08 Dow Agrociences Llc Produccion de inmunoglobulinas en plantas con una fucocilacion reducida
US7855063B2 (en) 2003-04-16 2010-12-21 Yeda Research And Development Co. Ltd. Partially deglycosylated glucocerebrosidase polypeptide and crystals thereof
WO2006035442A2 (en) 2004-09-29 2006-04-06 Collplant Ltd. Collagen producing plants and methods of generating and using same
WO2006040761A2 (en) 2004-10-15 2006-04-20 Oren Halpern A system and a method for improving the captured images of digital still cameras
NZ598048A (en) * 2006-01-17 2013-05-31 Synthon Biopharmaceuticals Bv Compositions and methods for humanization and optimization of n-glycans in plants
JP5568702B2 (ja) * 2006-11-08 2014-08-13 サントル ナショナル ドゥ ラ ルシェルシュ スィヤンティフィック（セーエヌエルエス） 組換えポリペプチドの発現および翻訳後修飾の制御を標的化するための配列を有するポリペプチド
WO2008128144A2 (en) * 2007-04-15 2008-10-23 Shuang Zhang Monoclonal antibody selecting system, and making and using thereof
SI2150608T1 (en) 2007-05-07 2018-04-30 Protalix Ltd. An extensive disposable bioreactor
HUE031698T2 (en) * 2007-06-15 2017-07-28 Medicago Inc Modification of glycoprotein production in plants
WO2009066303A2 (en) 2007-11-22 2009-05-28 Ganga Raju Gokaraju New synergistic phytochemical composition for the treatment of obesity
WO2009069123A2 (en) 2007-11-26 2009-06-04 Yissum Research Development Company Of The Hebrew University Of Jerusalem Compositions comprising fibrous polypeptides and polysaccharides
EP2090648A1 (en) * 2008-02-12 2009-08-19 Institut Francais de Recherche pour l'Exploitation de la Mere(Ifremer) Production of glycosylated polypeptides in microalgae
CA2733362C (en) * 2008-08-08 2016-10-18 Universiteit Gent Cells producing glycoproteins having altered glycosylation patterns and methods and use thereof
WO2010025285A1 (en) * 2008-08-27 2010-03-04 Arizona Board Of Regents For And On Behalf Of Arizona State University A dna replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants
WO2010138971A1 (en) 2009-05-29 2010-12-02 Edenspace Systems Corporation Plant gene regulatory elements
CA2777487A1 (en) * 2009-10-30 2011-05-05 Merck Sharp & Dohme Corp. Methods for the production of recombinant proteins with improved secretion efficiencies
EP2683799B1 (en) * 2011-03-07 2016-11-02 Agrivida, Inc. Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes
US11673926B2 (en) * 2011-06-07 2023-06-13 Ibio, Inc. In vivo de-glycosylation of recombinant proteins by co-expression with PNGase F
US20140090108A1 (en) * 2012-09-18 2014-03-27 Freydoun Garabagi Vectors and Methods For Enhancing Recombinant Protein Expression in Plants
US11555198B2 (en) * 2012-11-01 2023-01-17 Cellectis Sa Method for making nicotiana plants with mutations in XylT and FucT alleles using rare-cutting endonucleases
TWI745610B (zh) * 2012-11-14 2021-11-11 美商再生元醫藥公司 重組細胞表面捕捉蛋白質
AU2014227831B2 (en) * 2013-03-15 2020-01-30 Regents Of The University Of Minnesota Engineering plant genomes using CRISPR/Cas systems
US9689016B2 (en) * 2013-12-18 2017-06-27 Caliber Biotherapeutics, Llc Method for in vivo production of deglycosylated recombinant proteins used as substrate for downstream protein glycoremodeling

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US20230340516A1 (en) 2023-10-26
CN107810271B (zh) 2021-09-28
WO2016079739A2 (en) 2016-05-26
CN107810271A (zh) 2018-03-16
WO2016079739A9 (en) 2017-02-09
EP3221455B1 (en) 2020-08-12
US20170283822A1 (en) 2017-10-05
US11697819B2 (en) 2023-07-11
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EP3221455A2 (en) 2017-09-27
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